Abstract:
:The hepatic metabolism of deuteriated D-methionine has been studied in the intact, anesthetized rat using 2H NMR spectroscopy. The rate of formation of the principal labeled metabolite, [methyl-2H3]sarcosine, from the D-[methyl-2H3]methionine precursor was found to be as rapid as the rate observed previously in NMR studies of the hepatic metabolism of L-methionine. Similarly, rates of clearance of labeled methionine from the liver, formation of N-trimethyl-labeled metabolites, and labeling of the HDO pool were all found to be similar to the rates observed in the L-methionine studies. In contrast, all of these metabolic transformations are strongly inhibited by pretreatment of the rats with sodium benzoate, an inhibitor of D-amino acid oxidase. In vivo 2H NMR studies of sodium benzoate treated rats given L-[methyl-2H3]-methionine exhibit a much more rapid formation of [methyl-2H3]sarcosine than rats given the D enantiomer, consistent with the expectation that the sodium benzoate does not interfere with either the formation of S-adenosylmethionine or the subsequent transmethylation of glycine. However, the rates of methionine clearance and formation of deuteriated water are markedly reduced in this study relative to rats receiving the labeled D- or L-methionine without sodium benzoate pretreatment. These results indicate that subsequent to the initial oxidative deamination of the labeled D-methionine, the reamination to give L-methionine is rapid compared with the further degradation of the alpha-keto acid. Thus, the results are consistent with a dominant contribution of the glycine/sarcosine shuttle to the metabolism of excess D- or L-methionine.
journal_name
Biochemistryjournal_title
Biochemistryauthors
London RE,Gabel SAdoi
10.1021/bi00420a042subject
Has Abstractpub_date
1988-10-04 00:00:00pages
7864-9issue
20eissn
0006-2960issn
1520-4995journal_volume
27pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The isolated cytochrome (cyt) b(6)f complex from spinach is inhibited by Cu(2+) with a K(D) of about 1 microM at pH 7.6 in the presence of 1.6 microM decyl-plastoquinol (C(10)-PQH(2)) as a substrate. Inhibition was competitive with respect to C(10)-PQH(2) but noncompetitive with respect to horse heart cyt c or plastoc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991974a
更新日期:2000-03-28 00:00:00
abstract::Human liver alanine aminopeptidase is inhibited by L-amino acids having hydrophobic side chains such as Phe, Tyr, Trp, Met, and Leu. Blocking of the amino group or the carboxyl group greatly reduces the inhibitory capacity of the amino acid. Kinetic studies demonstrate that inhibition of hydrolysis of the substrate L-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00685a027
更新日期:1975-07-15 00:00:00
abstract::Femtosecond spectroscopy was performed on CO-liganded (fully reduced and mixed-valence states) and O(2)-liganded quinol oxidase bd from Escherichia coli. Substantial polarization effects, unprecedented for optical studies of heme proteins, were observed in the CO photodissociation spectra, implying interactions betwee...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0158019
更新日期:2002-02-05 00:00:00
abstract::Numerous studies have concluded that the interaction of the amyloid β-peptide (Aβ) and cellular membranes contributes to the toxicity and cell death observed in the progression of Alzheimer's disease. Aggregated Aβ species disrupt membranes, leading to physical instability and ion leakage. Further, the presence of Aβ ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400562x
更新日期:2013-07-23 00:00:00
abstract::Rhodanese bound to bacterial ribosomes as peptidyl-tRNA can be folded into an enzymatically active conformation by generating C-terminal extensions of the wild-type enzyme. Rhodanese was synthesized by coupled transcription/translation in a cell-free Escherichia coli system from plasmids containing the coding sequence...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00044a003
更新日期:1995-11-07 00:00:00
abstract::Vanadate can often bind to phosphoryl transfer enzymes to form a trigonal-bipyramidal structure at the active site. The enzyme-vanadate dissociation constants in these enzymes are much lower than those for phosphate. Therefore, enzyme-bound vanadate moieties are often considered as transition state analogues. To test ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi016097z
更新日期:2002-05-07 00:00:00
abstract::We investigated how the intrinsic sequence-dependent properties probed via the phosphate linkages (BI ↔ BII equilibrium) influence the preferred shape of free DNA, and how this affects the nucleosome formation. First, this exploits NMR solution studies of four B-DNA dodecamers that together cover 39 base pairs of the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500504y
更新日期:2014-09-09 00:00:00
abstract::T4 RNA ligase catalyzes the ATP-dependent addition of a single 2'-deoxyribonucleoside 3',5'-bisphosphate to the 3'-hydroxyl of an oligodeoxyribonucleotide. The bisphosphate is joined to the deoxyoligomer by a 3' leads to 5' phosphodiester bond and the product, which is terminated by a 3'-phosphate, is one nucleotide l...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00617a004
更新日期:1978-11-28 00:00:00
abstract::The kinetics of tert-butyl isocyanide binding to the heme protein horseradish peroxidase (HRP) at 22 degrees C was examined on all time scales, from minutes to picoseconds, in aqueous borate buffer at pH 9.08. Unlike myoglobin (Mb) or hemoglobin, HRP shows two bimolecular ligand binding processes. For comparison, bind...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9518149
更新日期:1996-02-06 00:00:00
abstract::Myeloperoxidase (MPO) structural analysis has suggested that halides and pseudohalides bind to the distal binding site and serve as substrates or inhibitors, while others have concluded that there are two separate sites. Here, evidence for two distinct binding sites for halides comes from the bell-shaped effects obser...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0609725
更新日期:2007-01-16 00:00:00
abstract::One way to improve the economic feasibility of biomass conversion is to enhance the catalytic efficiency of cellulases through protein engineering. This requires that high-resolution structures of cellulases be available. Here we present the structure of E2cd, the catalytic domain of the thermophilic endocellulase E2 ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00089a006
更新日期:1993-09-28 00:00:00
abstract::Human DNA is continuously damaged by exogenous and endogenous genotoxic insults. To counteract DNA damage and ensure the completion of DNA replication, cells possess specialized DNA polymerases (Pols) that bypass a variety of DNA lesions. Human DNA polymerase kappa (hPolkappa) is a member of the Y-family of DNA Pols a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900153t
更新日期:2009-05-26 00:00:00
abstract::The antimicrobial peptide MSI-78 serves as a model system for studying interactions of bioactive peptides with membranes. Using a series of MSI-78 peptides that incorporate l-4,4,4-trifluoroethylglycine, a small and sensitive (19)F nuclear magnetic resonance probe, we investigated how the local structure and dynamics ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200639c
更新日期:2011-07-12 00:00:00
abstract::The folding kinetics of a 16-residue beta-hairpin (trpzip4) and five mutants were studied by a laser-induced temperature-jump infrared method. Our results indicate that mutations which affect the strength of the hydrophobic cluster lead to a decrease in the thermal stability of the beta-hairpin, as a result of increas...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052039s
更新日期:2006-02-28 00:00:00
abstract::The prothrombinase complex assembles through reversible interactions between factor Xa, factor Va and acidic phospholipid-containing membranes in the presence of calcium ions. This complex catalyses the conversion of prothrombin to thrombin through two proteolytic steps. We have used prethrombin 2 as a substrate analo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9623993
更新日期:1997-03-18 00:00:00
abstract::The two distinct domains of flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase, EC 1.1.2.3) are connected by a typical hinge peptide. To probe the importance of the structural integrity of the hinge region for efficient intraprotein electron transfer, three mutant enzymes have been constructed: H delta 3 [Sharp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi950457z
更新日期:1996-01-23 00:00:00
abstract::The micellar properties of mixed, bovine gangliosides and purified galactosyl-N-acetylacetylgalactosaminyl (N-acetylneuraminyl) galactosylglucosylceramide were studied by gel filtration, equilibrium dialysis, and band and boundary centrifugation in sucrose gradients. The dissociation of micelles is very slow (days) in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00573a028
更新日期:1979-03-20 00:00:00
abstract::The serum high density lipoproteins (HDL) of normolipemic dogs (beagles) were isolated in the density range of p 1.063 to 1.21 g/ml, and characterized in terms of composition and physical properties (flotation and diffusion coefficients, partial specific volume, molecular weight, electrophoretic mobility, ultraviolet ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00654a022
更新日期:1976-05-04 00:00:00
abstract::An affinity labeling technique has been developed to identify the folate-methotrexate transporter of Leishmania donovani promastigotes using "activated" derivatives of the ligands. These "activated" derivatives were synthesized by incubating folate and methotrexate with a 10-fold excess of 1-ethyl-3-[3-(dimethylamino)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00443a023
更新日期:1989-08-22 00:00:00
abstract::Protein arginine methyltransferases (PRMTs) catalyze the transfer of methyl groups from S-adenosylmethionine (SAM) to the guanidinium group of arginine residues in a number of important cell signaling proteins. PRMT1 is the founding member of this family, and its activity appears to be dysregulated in heart disease an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi102022e
更新日期:2011-04-26 00:00:00
abstract::X-ray absorption spectroscopy has been used to investigate binding of selenohomocysteine to cobalamin-independent (MetE) and cobalamin-dependent (MetH) methionine synthase enzymes of Escherichia coli. We have shown previously [Peariso et al. (1998) J. Am. Chem. Soc. 120, 8410-8416] that the Zn sites in both enzymes sh...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001711c
更新日期:2001-01-30 00:00:00
abstract::Fluorescence recovery after photobleaching was used to measure the diffusion coefficient of green fluorescent protein (GFP, 27 kDa) in Escherichia coli in the presence or absence of four coexpressed proteins: cytoplasmic maltose binding protein (42 kDa), tau-40 (45 kDa), alpha-synuclein (14 kDa), or calmodulin (17 kDa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9004107
更新日期:2009-06-16 00:00:00
abstract::Cathepsin C, or dipeptidyl peptidase I, is a lysosomal cysteine protease of the papain family that catalyzes the sequential removal of dipeptides from the free N-termini of proteins and peptides. Using the dipeptide substrate Ser-Tyr-AMC, cathepsin C was characterized in both steady-state and pre-steady-state kinetic ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi8007627
更新日期:2008-08-19 00:00:00
abstract::The structure of an analogue of the yeast tRNAPhe T Psi C stem-loop has been determined by NMR spectroscopy and restrained molecular dynamics. The molecule contained the highly conserved modification ribothymidine at its naturally occurring position. The ribothymidine-modified T Psi C stem-loop is the product of the m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990118w
更新日期:1999-07-06 00:00:00
abstract::Acetohydroxyacid synthase (AHAS, EC 4.1.3.18) catalyzes the first step in the biosynthesis of the branched-chain amino acids. In bacteria, the enzyme has a large subunit containing the catalytic machinery and a small subunit with a regulatory role. In eucaryotes, the evidence for a regulatory subunit is largely indire...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi983013m
更新日期:1999-04-20 00:00:00
abstract::Detailed analysis of factors governing high affinity antibody-antigen interactions yields important insight into molecular recognition and facilitates the design of functional antibody libraries. Here we describe comprehensive mutagenesis of the light chain complementarity determining regions (CDRs) of HIV-1 antibody ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100293q
更新日期:2010-07-06 00:00:00
abstract::Doxorubicin (DOX) is a chemotherapeutic that is used in the treatment of a wide variety of cancers. However, it causes cardiotoxicity partly because of the formation of reactive oxygen species. CYP2J2 is a human cytochrome P450 that is strongly expressed in cardiomyocytes. It converts arachidonic acid (AA) into four d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01025
更新日期:2017-12-26 00:00:00
abstract::Ethylene and its analogues acetylene, carbon monoxide, and propylene inhibited the rate of oxidation of indole-3-acetic acid by peroxidase. Annulment of this effect by addition of superoxide dismutase showed that inhibition occurred only in the presence of the superoxide anion radical (O2-.). Kinetic and spectral data...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00276a019
更新日期:1983-03-29 00:00:00
abstract::NLRP4 is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family of cytosolic receptors and a member of an inflammation signaling cascade. Here, we present the crystal structure of the NLRP4 pyrin domain (PYD) at 2.3 Å resolution. The NLRP4 PYD is a member of the death domain (DD) superfamily ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3007059
更新日期:2012-09-18 00:00:00
abstract::The unfolding and refolding of alpha-helical proteins has been extensively studied, demonstrating formation of intermediate structures which retain the native-like alpha-helix but lack the tertiary structure. Studies on the folding of proteins consisting primarily of beta-sheet are interesting since, unlike the alpha-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952766v
更新日期:1996-09-03 00:00:00