High-pressure 31P NMR study of dipalmitoylphosphatidylcholine bilayers.

abstract：:Barbiturates inhibit GLUT-1 mediated hexose transport both in vivo [Gjedde & Rasmussen (1980) J. Neurochem. 35, 1382-1387; Otsuka et al. (1991) Am. J. Physiol. 261, R265-R275] and in vitro [Honkanen et al. (1995) Biochemistry 34, 535-544]. In the present study, the mechanism by which barbiturates inhibit GLUT-1 mediat...

journal_title：Biochemistry

authors： el-Barbary A,Fenstermacher JD,Haspel HC

更新日期：1996-12-03 00:00:00

abstract：:We used CD and UV resonance Raman spectroscopy to study the impact of alcohols on the conformational equilibria and relative Gibbs free energy landscapes along the Ramachandran Psi-coordinate of a mainly poly-Ala peptide, AP with an AAAAA(AAARA)(3)A sequence. 2,2,2-Trifluoroethanol (TFE) most stabilizes the alpha-heli...

journal_title：Biochemistry

authors： Xiong K,Asher SA

更新日期：2010-04-20 00:00:00

abstract：:The active site structures of human Q31 granzyme A, murine granzymes (A, B, C, D, E, and F), and human granzymes (A, B, and 3) isolated from cytotoxic T lymphocytes (CTL) were studied with peptide thioester substrates, peptide chloromethyl ketone, and isocoumarin inhibitors. Human Q31, murine, and human granzyme A hyd...

journal_title：Biochemistry

authors： Odake S,Kam CM,Narasimhan L,Poe M,Blake JT,Krahenbuhl O,Tschopp J,Powers JC

更新日期：1991-02-26 00:00:00

abstract：:Among the major obstacles to pharmacological and structural studies of integral membrane proteins (MPs) are their natural scarcity and the difficulty in overproducing them in their native form. MPs can be overexpressed in the non-native state as inclusion bodies, but inducing them to achieve their functional three-dim...

journal_title：Biochemistry

authors： Pocanschi CL,Dahmane T,Gohon Y,Rappaport F,Apell HJ,Kleinschmidt JH,Popot JL

更新日期：2006-11-28 00:00:00

abstract：:The transfer of ganglioside GM1 from micelles to membranes and between different membrane populations has been examined by using a pyrene fatty acid derivative of the ganglioside. The transfer of gangliosides from micelles to membranes depends on the physical state as well as the molecular composition of the acceptor ...

journal_title：Biochemistry

authors： Masserini M,Freire E

更新日期：1987-01-13 00:00:00

abstract：:We have characterized by EPR the interaction of the Em,7 = -50 mV [4Fe-4S] cluster of Escherichia coli DMSO reductase (DmsABC) with a menaquinol (MQH2) binding site. Potentiometric titrations indicate that in DmsAB(C102S)C, the Em,7 = -50 mV [4Fe-4S] cluster is replaced by an Em,7 = +260 mV [3Fe-4S] cluster. The Q-poo...

journal_title：Biochemistry

authors： Rothery RA,Weiner JH

更新日期：1996-03-12 00:00:00

abstract：:Leucine aminopeptidase (LAP) is a protease requiring two divalent metal cations per subunit for activity. Zn2+, Mg2+, and Co2+ metal-substituted forms of LAP have been prepared and investigated kinetically. Substitution of metal into the two binding sites independently resulted in the preparation of Zn2+Zn2+, Mg2+Zn2+...

journal_title：Biochemistry

authors： Allen MP,Yamada AH,Carpenter FH

更新日期：1983-08-02 00:00:00

abstract：:Biogenesis of pyrroloquinoline quinone (PQQ) in Klebsiella pneumoniae requires the expression of six genes (pqqA-F). One of these genes (pqqE) encodes a 43 kDa protein (PqqE) that plays a role in the initial steps in PQQ formation [Veletrop, J. S., et al. (1995) J. Bacteriol. 177, 5088-5098]. PqqE contains two highly ...

journal_title：Biochemistry

authors： Wecksler SR,Stoll S,Tran H,Magnusson OT,Wu SP,King D,Britt RD,Klinman JP

更新日期：2009-10-27 00:00:00

abstract：:4-Hydroxybenzoyl-CoA reductase (4-HBCR) is a member of the xanthine oxidase (XO) family of molybdenum cofactor containing enzymes and catalyzes the irreversible removal of a phenolic hydroxy group by reduction, yielding benzoyl-CoA and water. In this work the effects of various activity modulating compounds were chara...

journal_title：Biochemistry

authors： Johannes J,Unciuleac MC,Friedrich T,Warkentin E,Ermler U,Boll M

更新日期：2008-04-29 00:00:00

abstract：:The complex kinetic behavior commonly observed in protein folding studies suggests that a heterogeneous population of molecules exists in solution and that a number of discrete steps are involved in the conversion of unfolded molecules to the fully native form. A central issue in protein folding is whether any of thes...

journal_title：Biochemistry

authors： Walkenhorst WF,Green SM,Roder H

更新日期：1997-05-13 00:00:00

abstract：:The luminescence properties (fluorescence and phosphorescence) of luliberin have been investigated at liquid-nitrogen temperature (77 K) in 50% ethylene glycolaqueous buffer at various pH's. Calculation of the energy-transfer efficiency between Trp and Tyr residues leads to an evaluation of the distance separating the...

journal_title：Biochemistry

authors： Marche P,Montenay-Garestier T,Fromageot P,Hélène C

更新日期：1976-12-28 00:00:00

abstract：:To maintain daily cycles, the circadian clock must tightly regulate the rhythms of thousands of mRNAs and proteins with the correct period, phase, and amplitude to ultimately drive the wide range of rhythmic biological processes. Recent genomic approaches have revolutionized our view of circadian gene expression and h...

journal_title：Biochemistry

authors： Kojima S,Green CB

更新日期：2015-01-20 00:00:00

abstract：:S-Adenosylmethionine (AdoMet) synthetase catalyzes the only known route of biosynthesis of the primary in vivo alkylating agent. Inhibitors of this enzyme could provide useful modifiers of biological methylation and polyamine biosynthetic processes. The AdoMet synthetase catalyzed reaction converts ATP and L-methionin...

journal_title：Biochemistry

authors： Reczkowski RS,Markham GD

更新日期：1999-07-13 00:00:00

abstract：:Photoactive yellow proteins (PYPs) make up a diverse class of blue-light-absorbing bacterial photoreceptors. Electronic excitation of the p-coumaric acid chromophore covalently bound within PYP results in triphasic quenching kinetics; however, the molecular basis of this behavior remains unresolved. Here we explore th...

journal_title：Biochemistry

authors： Mix LT,Carroll EC,Morozov D,Pan J,Gordon WR,Philip A,Fuzell J,Kumauchi M,van Stokkum I,Groenhof G,Hoff WD,Larsen DS

更新日期：2018-03-20 00:00:00

abstract：:The sensitivity of the fluorescence energy of 2-p-toluidinylnaphthalene-6-sulfonate (TNS) to apparent solvent polarity is considerably greater in methanol-water mixtures and lower primary alcohols than in higher primary alcohols or solvents containing no hydroxyl groups. It is suggested that the behavior of TNS in low...

journal_title：Biochemistry

authors： Greene FC

更新日期：1975-02-25 00:00:00

abstract：:Human S100B(beta beta) is a small intracellular EF-hand calcium-binding protein that consists of two noncovalently associated 91-residue beta monomers. The three-dimensional structures of S100B reveal the dimer interface consists of four alpha-helices (I, I' and IV, IV') packed in an X-type bundle. In this study, guan...

journal_title：Biochemistry

authors： Ferguson PL,Shaw GS

更新日期：2002-03-19 00:00:00

abstract：:Trifluoperazine (TFP) was shown to interact with the cyanogen bromide fragment 9 (CB9) (residues 84-135) of rabbit skeletal troponin C and with a synthetic peptide representing the N-terminal region of CB9. The phenothiazine did not affect the calcium binding property of CB9 as observed by proton magnetic resonance an...

journal_title：Biochemistry

authors： Gariépy J,Hodges RS

更新日期：1983-03-29 00:00:00

abstract：:Neuroglobin (Ngb) is a recently discovered vertebrate heme protein that is expressed in the brain and can reversibly bind oxygen. Mammalian Ngb is involved in neuroprotection under oxidative stress conditions, such as ischemia and reperfusion. We previously demonstrated that human ferric Ngb binds to the alpha subunit...

journal_title：Biochemistry

authors： Watanabe S,Wakasugi K

更新日期：2008-05-13 00:00:00

abstract：:Several enzymatic and chemical reagents were used to probe the secondary structure of Saccharomyces cerevisiae nuclear RNase P RNA in the presence and absence of its protein components. Double-stranded regions were detected with RNase V1 and single-stranded regions with RNase ONE (Escherichia coli RNase I). Nucleotide...

journal_title：Biochemistry

authors： Tranguch AJ,Kindelberger DW,Rohlman CE,Lee JY,Engelke DR

更新日期：1994-02-22 00:00:00

abstract：:The central importance of chorismate enzymes in bacteria, fungi, parasites, and plants combined with their absence in mammals makes them attractive targets for antimicrobials and herbicides. Two of these enzymes, anthranilate synthase (AS) and aminodeoxychorismate synthase (ADCS), are structurally and mechanistically ...

journal_title：Biochemistry

authors： Culbertson JE,Chung Dh,Ziebart KT,Espiritu E,Toney MD

更新日期：2015-04-14 00:00:00

abstract：:The O-linked protein glycosylation pathway in Neisseria gonorrhoeae is responsible for the synthesis of a complex oligosaccharide on undecaprenyl diphosphate and subsequent en bloc transfer of the glycan to serine residues of select periplasmic proteins. Protein glycosylation (pgl) genes have been annotated on the bas...

journal_title：Biochemistry

authors： Hartley MD,Morrison MJ,Aas FE,Børud B,Koomey M,Imperiali B

更新日期：2011-06-07 00:00:00

abstract：:Glutamate semialdehyde aminomutase is a recognized target for selective herbicides and antibacterial agents because it provides the aminolevulinate from which tetrapyrroles are synthesized in plants and bacteria but not in animals. The reactions of the enzyme with R- and S-enantiomers of a novel compound, diaminopropy...

journal_title：Biochemistry

authors： Contestabile R,Jenn T,Akhtar M,Gani D,John RA

更新日期：2000-03-21 00:00:00

abstract：:X-ray absorption spectroscopy has been used to investigate binding of selenohomocysteine to cobalamin-independent (MetE) and cobalamin-dependent (MetH) methionine synthase enzymes of Escherichia coli. We have shown previously [Peariso et al. (1998) J. Am. Chem. Soc. 120, 8410-8416] that the Zn sites in both enzymes sh...

journal_title：Biochemistry

authors： Peariso K,Zhou ZS,Smith AE,Matthews RG,Penner-Hahn JE

更新日期：2001-01-30 00:00:00

abstract：:Despite the high degree of sequence similarity in their basic-helix-loop-helix (BHLH) domains, MASH-1 and MyoD are involved in different biological processes. In order to define possible differences between the DNA binding specificities of these two proteins, we investigated the DNA binding properties of MASH-1 by cir...

journal_title：Biochemistry

authors： Meierhan D,el-Ariss C,Neuenschwander M,Sieber M,Stackhouse JF,Allemann RK

更新日期：1995-09-05 00:00:00

abstract：:The oxidation of alcohol to aldehyde by horse liver alcohol dehydrogenase (LADH) requires the transfer of a hydride ion from the alcohol substrate to the cofactor nicotinamide adenine dinucleotide (NAD). A quantum mechanical tunneling contribution to this hydride transfer step has been demonstrated in a number of LADH...

journal_title：Biochemistry

authors： Colby TD,Bahnson BJ,Chin JK,Klinman JP,Goldstein BM

更新日期：1998-06-30 00:00:00

abstract：:The bleomycins (BLMs), tallysomycins (TLMs), phleomycin, and zorbamycin (ZBM) are members of the BLM family of glycopeptide-derived antitumor antibiotics. The BLM-producing Streptomyces verticillus ATCC15003 and the TLM-producing Streptoalloteichus hindustanus E465-94 ATCC31158 both possess at least two self-resistanc...

journal_title：Biochemistry

authors： Rudolf JD,Bigelow L,Chang C,Cuff ME,Lohman JR,Chang CY,Ma M,Yang D,Clancy S,Babnigg G,Joachimiak A,Phillips GN Jr,Shen B

更新日期：2015-11-17 00:00:00

abstract：:Polynucleosomes with poly(dG-m5dC).poly(dG-m5dC) have been reconstituted, and well-defined nucleosome core particles from these have been prepared. Upon addition of MgCl2 to the levels used to induce the B to Z transition in this highly methylated DNA, significant changes in the circular dichroism spectrum are observe...

journal_title：Biochemistry

authors： Ausio J,Zhou G,van Holde K

更新日期：1987-09-08 00:00:00

abstract：:Saccharomyces cerevisiae myristoylCoA:protein N-myristoyltransferase (Nmt1p) is an essential enzyme that catalyzes the transfer of myristic acid (C14:0) from myristoylCoA to the N-terminus of cellular proteins with a variety of functions. Nmts from an assortment of species display remarkable in vivo specificity for th...

journal_title：Biochemistry

authors： Bhatnagar RS,Schall OF,Jackson-Machelski E,Sikorski JA,Devadas B,Gokel GW,Gordon JI

更新日期：1997-06-03 00:00:00

abstract：:LL-23 is a natural peptide corresponding to the 23 N-terminal amino acid residues of human host defense cathelicidin LL-37. LL-23 demonstrated, compared to LL-37, a conserved ability to induce the chemokine MCP-1 in human peripheral blood mononuclear cells, a lack of ability to suppress induction of the pro-inflammato...

journal_title：Biochemistry

authors： Wang G,Elliott M,Cogen AL,Ezell EL,Gallo RL,Hancock RE

更新日期：2012-01-17 00:00:00

abstract：:Rhodopsin is the photoreceptor in vertebrate rod cells responsible for vision at low light intensities. The photoreactive chromophore in rhodopsin is 11-cis-retinal bound to the protein via a protonated Schiff base with Glu113 as the counterion. We have used the observed 13C NMR chemical shifts of the conjugated retin...

journal_title：Biochemistry

authors： Han M,Smith SO

更新日期：1995-01-31 00:00:00