当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Pyrroloquinoline quinone biogenesis: demonstration that PqqE from Klebsiella pneumoniae is a radical S-adenosyl-L-methionine enzyme.

Pyrroloquinoline quinone biogenesis: demonstration that PqqE from Klebsiella pneumoniae is a radical S-adenosyl-L-methionine enzyme.

Abstract：

:Biogenesis of pyrroloquinoline quinone (PQQ) in Klebsiella pneumoniae requires the expression of six genes (pqqA-F). One of these genes (pqqE) encodes a 43 kDa protein (PqqE) that plays a role in the initial steps in PQQ formation [Veletrop, J. S., et al. (1995) J. Bacteriol. 177, 5088-5098]. PqqE contains two highly conserved cysteine motifs at the N- and C-termini, with the N-terminal motif comprised of a CX(3)CX(2)C consensus sequence that is unique to a family of proteins known as radical S-adenosyl-l-methionine (SAM) enzymes [Sofia, H. J., et al. (2001) Nucleic Acids Res. 29, 1097-1106]. PqqE from K. pneumoniae was cloned into Escherichia coli and expressed as the native protein and with an N-terminal His(6) tag. Anaerobic expression and purification of the His(6)-tagged PqqE results in an enzyme with a brownish-red hue indicative of Fe-S cluster formation. Spectroscopic and physical analyses indicate that PqqE contains a mixture of Fe-S clusters, with the predominant form of the enzyme containing two [4Fe-4S] clusters. PqqE isolated anaerobically yields an active enzyme capable of cleaving SAM to methionine and 5'-deoxyadenosine in an uncoupled reaction (k(obs) = 0.011 +/- 0.001 min(-1)). In this reaction, the 5'-deoxyadenosyl radical either abstracts a hydrogen atom from a solvent accessible position in the enzyme or obtains a proton and electron from buffer. The putative PQQ substrate PqqA has not yet been shown to be modified by PqqE, implying that PqqA must be modified before becoming the substrate for PqqE and/or that another protein in the biosynthetic pathway is critical for the initial steps in PQQ biogenesis.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Wecksler SR,Stoll S,Tran H,Magnusson OT,Wu SP,King D,Britt RD,Klinman JP

doi

10.1021/bi900918b

subject

Has Abstract

pub_date

2009-10-27 00:00:00

pages

10151-61

issue

42

eissn

0006-2960

issn

1520-4995

journal_volume

48

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Life and Work of Stress Granules and Processing Bodies: New Insights into Their Formation and Function.

    abstract：:The dynamic formation of stress granules (SGs), processing bodies (PBs), and related RNA organelles regulates diverse cellular processes, including the coordination of functionally connected messengers, the translational regulation at the synapse, and the control of viruses and retrotransposons. Recent studies have sh...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00025

    authors： Perez-Pepe M,Fernández-Alvarez AJ,Boccaccio GL

    更新日期：2018-05-01 00:00:00

  • Antiplatelet "hybrid" peptides analogous to receptor recognition domains on gamma and alpha chains of human fibrinogen.

    abstract：:Platelet receptor recognition domains are located on the gamma and alpha chains of human fibrinogen. The former encompasses residues 400-411 [Kloczewiak, M., Timmons, S., Lukas, T. J., & Hawiger, J. (1984) Biochemistry 23, 1767], and the latter is present in two loci on the alpha chain (alpha 95-97 and alpha 572-574) ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00433a026

    authors： Timmons S,Bednarek MA,Kloczewiak M,Hawiger J

    更新日期：1989-04-04 00:00:00

  • A multifunctional protein possessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotide transformylase, and aminoimidazole ribonucleotide synthetase activities in de novo purine biosynthesis.

    abstract：:Three activities on the pathway of purine biosynthesis de novo in chicken liver, namely, glycinamide ribonucleotide synthetase, glycinamide ribonucleotide transformylase, and aminoimidazole ribonucleotide synthetase, have been found to reside on the same polypeptide chain. Three diverse purification schemes, utilizing...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00346a006

    authors： Daubner SC,Schrimsher JL,Schendel FJ,Young M,Henikoff S,Patterson D,Stubbe J,Benkovic SJ

    更新日期：1985-12-03 00:00:00

  • Aggregation behaviour and Zn2+ binding properties of secretin.

    abstract：:Dynamic light scattering measurements were carried out on secretin in aqueous solution (2 mM; pH 5.0). The results indicated that the molecule exists as a fairly compact hexamer under these solution conditions. Secondary structural properties of the secretin hexameric complex were evaluated using CD and NMR spectrosco...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980701x

    authors： Carpenter KA,Schiller PW

    更新日期：1998-12-01 00:00:00

  • Histidine residues at the N- and C-termini of alpha-helices: perturbed pKas and protein stability.

    abstract：:A single histidine residue has been placed at either the N-terminus or the C-terminus of each of the two alpha-helices of barnase. The pKa of that histidine residue in each of the four mutants has been determined by 1H NMR. The pKas of the two residues at the C-terminus are, on average, 0.5 unit higher, and those of t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00123a006

    authors： Sancho J,Serrano L,Fersht AR

    更新日期：1992-03-03 00:00:00

  • Glycosylinositol phospholipid anchors of the scrapie and cellular prion proteins contain sialic acid.

    abstract：:The only identified component of the scrapie prion is PrPSc, a glycosylinositol phospholipid (GPI)-linked protein that is derived from the cellular isoform (PrPC) by an as yet unknown posttranslational event. Analysis of the PrPSc GPI has revealed six different glycoforms, three of which are unprecedented. Two of the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00136a600

    authors： Stahl N,Baldwin MA,Hecker R,Pan KM,Burlingame AL,Prusiner SB

    更新日期：1992-06-02 00:00:00

  • Role of glycosylation on the secretion and biological activity of erythropoietin.

    abstract：:The erythropoietin (EPO) molecule contains four carbohydrate chains. Three contain N-linkages to asparagines at positions 24, 38, and 83, and one contains an O-linkage to a serine at position 126. We constructed human EPO variants that eliminated the three N-glycosylation sites by replacing the asparagines with glutam...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00156a003

    authors： Delorme E,Lorenzini T,Giffin J,Martin F,Jacobsen F,Boone T,Elliott S

    更新日期：1992-10-20 00:00:00

  • Competition between Fibrillation and Induction of Vesicle Fusion for the Membrane-Associated 40-Residue β-Amyloid Peptides.

    abstract：:Disruption of the cell membrane by the β-amyloid (Aβ) peptides has been considered as a main mechanism of Alzheimer's disease. The peptide-to-lipid molar ratio (P:L) varies over a broad range biologically. We report here that two of the previously observed Aβ evolution pathways, fibrillation and induction of vesicle f...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00321

    authors： Akinlolu RD,Nam M,Qiang W

    更新日期：2015-06-09 00:00:00

  • A test of the linear extrapolation of unfolding free energy changes over an extended denaturant concentration range.

    abstract：:Guanidine hydrochloride (GdnHCl) and thermally induced unfolding measurements on the oxidized form of Escherichia coli thioredoxin at pH 7 were combined for the purpose of assessing the functional dependence of unfolding free energy changes on denaturant concentration over an extended GdnHCl concentration range. Conve...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00135a022

    authors： Santoro MM,Bolen DW

    更新日期：1992-05-26 00:00:00

  • Two disaccharides and trimethylamine N-oxide affect Abeta aggregation differently, but all attenuate oligomer-induced membrane permeability.

    abstract：:Interaction between aggregates of amyloid beta protein (Abeta) and membranes has been hypothesized by many to be a key event in the mechanism of neurotoxicity associated with Alzheimer's disease (AD). Proposed membrane-related mechanisms of neurotoxicity include ion channel formation, membrane disruption, changes in m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9006397

    authors： Qi W,Zhang A,Good TA,Fernandez EJ

    更新日期：2009-09-22 00:00:00

  • Investigation of complexes formed between gene 32 protein from bacteriophage T4 and heavy-atom-modified single-stranded polynucleotides using optical detection of magnetic resonance.

    abstract：:Optical detection of triplet-state magnetic resonance (ODMR) is employed to study the complexes formed between gene 32 protein (GP32), a single-stranded DNA-binding protein from bacteriophage T4, and the heavy-atom-derivatized polynucleotides poly(5-HgU) and poly(5-BrU). The triplet-state properties of some of the try...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00368a005

    authors： Khamis MI,Maki AH

    更新日期：1986-10-07 00:00:00

  • Short sequences of non-proline residues can adopt the polyproline II helical conformation.

    abstract：:The left-handed polyproline II (P(II)) helix is a structure that has been given a great deal of attention lately because of its role in a wide variety of physiologically important processes and potential significance in protein unfolded states. Recent work by several authors has shown that residues besides proline can...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049922v

    authors： Chellgren BW,Creamer TP

    更新日期：2004-05-18 00:00:00

  • Delta T 14/Delta D 15 Azotobacter vinelandii ferredoxin I: creation of a new CysXXCysXXCys motif that ligates a [4Fe-4S] cluster.

    abstract：:In clostridial-type ferredoxins, each of the two [4Fe-4S]2+/+ clusters receives three of its four ligands from a CysXXCysXXCys motif. Azotobacter vinelandii ferredoxin I (AvFdI) is a seven-iron ferredoxin that contains one [4Fe-4S]2+/+ cluster and one [3Fe-4S]+/0 cluster. During the evolution of the 7Fe azotobacter-ty...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9810499

    authors： Kemper MA,Gao-Sheridan HS,Shen B,Duff JL,Tilley GJ,Armstrong FA,Burgess BK

    更新日期：1998-09-15 00:00:00

  • Selection of a carbohydrate-binding domain with a helix-loop-helix structure.

    abstract：:We obtained a novel carbohydrate-binding peptide having a helix-loop-helix scaffold from a random peptide library. The helix-loop-helix peptide library randomized at five amino acid residues was displayed on the major coat protein of a filamentous phage. Affinity selection with a ganglioside, Galbeta1-3GalNAcbeta1-4(N...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8000837

    authors： Matsubara T,Iida M,Tsumuraya T,Fujii I,Sato T

    更新日期：2008-07-01 00:00:00

  • Solution structure of a DNA dodecamer containing the anti-neoplastic agent arabinosylcytosine: combined use of NMR, restrained molecular dynamics, and full relaxation matrix refinement.

    abstract：:The effect of araC incorporation into the dodecamer duplex [d(CGCGAATT) (araC)d(GCG)]2 was examined by comparing its nuclear magnetic resonance (NMR)-determined solution structure with that of the control duplex d[(CGCGAATTCGCG)]2. 1H and 31P resonances in both duplexes were assigned using a combination of 2-D 1H NMR ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：

    authors： Schweitzer BI,Mikita T,Kellogg GW,Gardner KH,Beardsley GP

    更新日期：1994-09-27 00:00:00

  • Interaction of lipoprotein lipase with phospholipid vesicles: effect on protein and lipid structure.

    abstract：:The interaction of lipoprotein lipase (LpL) and a nonhydrolyzable phosphatidylcholine, 1,2-ditetradecyl-rac-glycero-3-phosphocholine (C14-ether-PC), has been studied by several physical methods. Analysis of the circular dichroic spectrum of LpL gave the following fractional conformation: 35% alpha-helix, 30% beta-plea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00352a020

    authors： McLean LR,Larsen WJ,Jackson RL

    更新日期：1986-02-25 00:00:00

  • Improvement by benzoquinones of the quantum yield of photoactivation of photosynthetic oxygen evolution: direct evidence for the two-quantum mechanism.

    abstract：:Effects of eight differently substituted 1,4-benzoquinones (BQs) on the quantum yield of photoactivation of oxygen evolution (reconstitution of the Mn cluster) were examined with wheat photosystem II (PSII) membranes depleted of the Mn cluster by treatment with 1.0 mM NH2OH. Illumination with 10 flashes at 0.25-s inte...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00117a032

    authors： Miyao-Tokutomi M,Inoue Y

    更新日期：1992-01-21 00:00:00

  • Binding of adenosine diphosphoribosyltransferase to the termini and internal regions of linear DNAs.

    abstract：:Binding mechanisms of ADPR-transferase to restricted double-stranded DNA fragments of SV40 and pBR322 DNA were determined by nuclease protection techniques. Top and bottom strands of double-stranded DNA were identified by specific labeling with 32P. Protection against specific exonucleases identified binding of ADPR-t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00439a050

    authors： Sastry SS,Buki KG,Kun E

    更新日期：1989-06-27 00:00:00

  • Replacement of a conserved proline and the alkaline conformational change in iso-2-cytochrome c.

    abstract：:Although point mutations usually lead to minor localized changes in protein structure, replacement of conserved Pro-76 with Gly in iso-2-cytochrome c induces a major conformational change. The change in structure results from mutation-induced depression of the pK for transition to an alkaline conformation with altered...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00451a043

    authors： Nall BT,Zuniga EH,White TB,Wood LC,Ramdas L

    更新日期：1989-12-12 00:00:00

  • Potassium flux through gramicidin ion channels is augmented in vesicles comprised of plasmenylcholine: correlations between gramicidin conformation and function in chemically distinct host bilayer matrices.

    abstract：:The functional role of distinct phospholipid subclasses and molecular species in modulating gramicidin-mediated K+ flux was characterized through quantification of changes in the fluorescence intensity of ion specific fluorescent probes encapsulated inside vesicles comprised of individual molecular species of plasmeny...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00022a008

    authors： Chen X,Gross RW

    更新日期：1995-06-06 00:00:00

  • Chloroquine transport in Plasmodium falciparum. 1. Influx and efflux kinetics for live trophozoite parasites using a novel fluorescent chloroquine probe.

    abstract：:Several models for how amino acid substitutions in the Plasmodium falciparum chloroquine resistance transporter (PfCRT) confer resistance to chloroquine (CQ) and other antimalarial drugs have been proposed. Distinguishing between these models requires detailed analysis of high-resolution CQ transport data that is unfo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi901034r

    authors： Cabrera M,Natarajan J,Paguio MF,Wolf C,Urbach JS,Roepe PD

    更新日期：2009-10-13 00:00:00

  • Mechanistic studies of two dioxygenases in the methionine salvage pathway of Klebsiella pneumoniae.

    abstract：:Two dioxygenases (ARD and ARD') were cloned from Klebsiella pneumoniae that catalyze different oxidative decomposition reactions of an advanced aci-reductone intermediate, CH(3)SCH(2)CH(2)COCH(OH)=CH(OH) (I), in the methionine salvage pathway. The two enzymes are remarkable in that they have the same polypeptide seque...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010110y

    authors： Dai Y,Pochapsky TC,Abeles RH

    更新日期：2001-05-29 00:00:00

  • C5 cytosine methylation at CpG sites enhances sequence selectivity of mitomycin C-DNA bonding.

    abstract：:We have established that UvrABC nuclease is equally efficient in cutting mitomycin C (MC)-DNA monoadducts formed at different sequences and that the degree of UvrABC cutting represents the extent of drug-DNA bonding. Using this method we determined the effect of C5 cytosine methylation on the DNA monoalkylation by MC ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991307h

    authors： Li VS,Reed M,Zheng Y,Kohn H,Tang M

    更新日期：2000-03-14 00:00:00

  • NMR characterization of substrate binding in the phthalate dioxygenase system.

    abstract：:The paramagnetic enhancements in the NMR relaxation rates for the fluorine in fluorophthalates have been used to determine the position of the phthalate with respect to the mononuclear metal ion in native and metal-substituted derivatives of phthalate dioxygenase (PDO). These studies show directly that the substrate i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990431y

    authors： Tierney DL,Gassner GT,Luchinat C,Bertini I,Ballou DP,Penner-Hahn JE

    更新日期：1999-08-24 00:00:00

  • Elucidation of the structural basis for the slow reactivity of thrombin with plasminogen activator inhibitor-1.

    abstract：:Plasminogen activator inhibitor-1 (PAI-1) is a serine protease inhibitor of the serpin superfamily which rapidly inactivates tissue plasminogen activator (tPA), but reacts with thrombin at a much slower rate. Based on the previous mutagenesis studies and the X-ray crystal structure of the thrombin E192Q-bovine pancrea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9808518

    authors： Rezaie AR

    更新日期：1998-09-22 00:00:00

  • Functional properties of the histidine-aspartate ion pair of flavocytochrome b2 (L-lactate dehydrogenase): substitution of Asp282 with asparagine.

    abstract：:The FMN prosthetic group of flavocytochrome b2 or L-lactate dehydrogenase oxidizes lactate to pyruvate. The reducing equivalents are then transferred one by one, intramolecularly, to heme b2 and then to external acceptors. Substrate oxidation is thought to begin with abstraction of the substrate alpha-hydrogen as a pr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9529519

    authors： Gondry M,Lederer F

    更新日期：1996-07-02 00:00:00

  • Mammalian folylpoly-gamma-glutamate synthetase. 1. Purification and general properties of the hog liver enzyme.

    abstract：:Folylpolyglutamate synthetase was purified 30,000-150,000-fold from hog liver. Purification required the use of protease inhibitors, and the protein was purified to homogeneity in two forms. Both forms of the enzyme were monomers of Mr 62,000 and had similar specific activities. The specific activity of the homogeneou...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00376a024

    authors： Cichowicz DJ,Shane B

    更新日期：1987-01-27 00:00:00

  • Strongly altered receptor binding properties in PP and NPY chimeras are accompanied by changes in structure and membrane binding.

    abstract：:Neuropeptide Y (NPY) and the pancreatic polypeptide (PP) are members of the neuropeptide Y family of hormones. They bind to the Y receptors with very different affinities: Whereas PP is highly selective for the Y(4) receptor, NPY displays highest affinites for Y(1), Y(2), and Y(5) receptor subtypes. Introducing the NP...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0501232

    authors： Lerch M,Kamimori H,Folkers G,Aguilar MI,Beck-Sickinger AG,Zerbe O

    更新日期：2005-06-28 00:00:00

  • Hydrolysis of dihydrouridine and related compounds.

    abstract：:Dihydrouridine is absent from the tRNA of almost all hyperthermophiles and most Archaea but is ubiquitous in the tRNA of Eubacteria and Eukaryotes. In order to investigate whether this could be due to instability, the rate of ring opening of dihydrouridine was measured between 25 and 120 degrees C. The dihydrouridine ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi951577+

    authors： House CH,Miller SL

    更新日期：1996-01-09 00:00:00

  • Comparative 2D NMR studies of human insulin and des-pentapeptide insulin: sequential resonance assignment and implications for protein dynamics and receptor recognition.

    abstract：:The solution structure and dynamics of human insulin are investigated by 2D 1H NMR spectroscopy in reference to a previously analyzed analogue, des-pentapeptide(B26-B30) insulin (DPI; Hua, Q.X., & Weiss, M.A. (1990) Biochemistry 29, 10545-10555). This spectroscopic comparison is of interest since (i) the structure of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00236a025

    authors： Hua QX,Weiss MA

    更新日期：1991-06-04 00:00:00