Abstract:
:The only identified component of the scrapie prion is PrPSc, a glycosylinositol phospholipid (GPI)-linked protein that is derived from the cellular isoform (PrPC) by an as yet unknown posttranslational event. Analysis of the PrPSc GPI has revealed six different glycoforms, three of which are unprecedented. Two of the glycoforms contain N-acetylneuraminic acid, which has not been previously reported as a component of any GPI. The largest form of the GPI is proposed to have a glycan core consisting of Man alpha-Man alpha-Man-(NeuAc-Gal-GalNAc-)Man-GlcN-Ino. Identical PrPSc GPI structures were found for two distinct isolates or "strains" of prions which specify different incubation times, neuropathology, and PrPSc distribution in brains of Syrian hamsters. Limited analysis of the PrPC GPI reveals that it also has sialylated glycoforms, arguing that the presence of this monosaccharide does not distinguish PrPC from PrPSc.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Stahl N,Baldwin MA,Hecker R,Pan KM,Burlingame AL,Prusiner SBdoi
10.1021/bi00136a600subject
Has Abstractpub_date
1992-06-02 00:00:00pages
5043-53issue
21eissn
0006-2960issn
1520-4995journal_volume
31pub_type
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