Abstract:
:The sensitivity of the fluorescence energy of 2-p-toluidinylnaphthalene-6-sulfonate (TNS) to apparent solvent polarity is considerably greater in methanol-water mixtures and lower primary alcohols than in higher primary alcohols or solvents containing no hydroxyl groups. It is suggested that the behavior of TNS in lower alcohols and their mixtures with water is an anomalous result of the combination of general polarity influences and chanes in the nature of specific interactions of the probe with hydroxyl groups of such solvents. Upon conversion of the sulfonate group of TNS to sulfonamido, the biphasic behavior observed in alcohols is eliminated, and fluorescent probes are obtained which have more nearly equal sensitivites to polarity in the alcohols and nonhydroxyl solvents. The sulfonamido fluorescent probes are more sensitive to general environmental polarity than TNS, and are superior as probes of environmental polarity.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Greene FCdoi
10.1021/bi00675a016subject
Has Abstractpub_date
1975-02-25 00:00:00pages
747-53issue
4eissn
0006-2960issn
1520-4995journal_volume
14pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Human ferrochelatase (EC 4.99.1.1) catalyzes the insertion ferrous iron into protoporphyrin IX as the last step in heme biosynthesis, an essential process to most organisms given the vast intracellular functions of heme. Even with multiple ferrochelatase structures available, the exact mechanism for iron insertion int...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300704c
更新日期:2012-09-11 00:00:00
abstract::RNA repeat expansions cause a host of incurable, genetically defined diseases. The most common class of RNA repeats consists of trinucleotide repeats. These long, repeating transcripts fold into hairpins containing 1 × 1 internal loops that can mediate disease via a variety of mechanism(s) in which RNA is the central ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00252
更新日期:2017-07-11 00:00:00
abstract::The gene coding for the enzyme 2,3-dihydroxybenzoate-AMP ligase (2,3DHB-AMP ligase), responsible for activating 2,3-dihydroxybenzoic acid in the biosynthesis of the siderophore enterobactin, has been subcloned into the multicopy plasmid pKK223-3 and overproduced in a strain of Escherichia coli. The protein is an alpha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00443a008
更新日期:1989-08-22 00:00:00
abstract::Near-infrared FT-Raman spectroscopy can be used to measure the vibrations of the bacteriorhodopsin (bR) chromophore without the disadvantage of conventional visible resonance Raman spectroscopy, where the visible excitation drives the bR photoreactions. We utilized this technique to investigate the light-dark adaptati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00060a020
更新日期:1993-03-09 00:00:00
abstract::Apolipoprotein E (apoE) isoforms are key determinants of susceptibility to late-onset Alzheimer's disease (AD). The epsilon 4 and epsilon 2 alleles have been associated with increased and decreased risk for AD, respectively. We have generated and characterized transgenic mice in which the human apoE2 gene is expressed...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015971l
更新日期:2002-07-30 00:00:00
abstract::The specificity of subtilisin BPN' toward substrates with large hydrophobic P4 residues has been improved by single amino acid replacements at positions 104 and 107. Mutations were designed to (i) increase the size of the P4 binding pocket by replacing Ile107, which is at the bottom of the S4 pocket, by Val, Ala, and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00056a001
更新日期:1993-02-09 00:00:00
abstract::The tertiary structure of apolipoprotein (apo) A-I and the contributions of structural domains to the properties of the protein molecule are not well defined. We used a series of engineered human and mouse apoA-I molecules in a range of physical-biochemical measurements to address this issue. Circular dichroism measur...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi702332b
更新日期:2008-02-19 00:00:00
abstract::Membrane vesicles from Mycobacterium phlei contain carrier proteins for proline, glutamine, and glutamic acid. The transport of proline is Na+ dependent and required substrate oxidation. A proline carrier protein was solubilized from the membrane vesicles by treatment with cholate and Triton X-100. Electron microscopi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00578a015
更新日期:1979-05-29 00:00:00
abstract::In an attempt to understand the mechanism by which transcription factors compete with histone octamers for cognate binding sites in chromatin, the effect of the histone binding protein nucleoplasmin on the binding of TFIIIA to nucleosomal 5S rRNA genes was tested. In this study, it was shown that, despite the previous...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972723+
更新日期:1998-02-03 00:00:00
abstract::Calmodulin (CaM) is a small acidic protein essential to calcium-mediated signal transduction. Conformational change driven by calcium binding controls its selective activation of myriad target proteins. In most well characterized cases, both homologous domains of CaM interact with a target protein. However, physiologi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0023091
更新日期:2001-01-30 00:00:00
abstract::Self-assembly of beta-sheet domains resulting in the formation of pathogenic, fibrillar protein aggregates (amyloids) is a characteristic feature of various medical disorders. These include neurodegenerative diseases, such as Alzheimer's, Huntington's, and Creutzfeldt-Jacob's. A significant problem in studying such ag...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990510+
更新日期:1999-06-29 00:00:00
abstract::We have investigated the occurrence of cyclic electron flow in intact spinach leaves. In particular, we have tested the hypothesis that cyclic flow requires the presence of supercomplexes in the thylakoid membrane or other strong associations between proteins. Using biochemical approaches, we found no evidence of the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061439s
更新日期:2006-11-14 00:00:00
abstract::In order to further characterize the binding of the hybrid molecule NetOPC [bis(pyrrolecarboxamide)-oxazolopyridocarbazole conjugate] to double-stranded DNA, we have performed a molecular modeling study to investigate the binding modes of the complexes possibly formed between NetOPC and synthetic polynucleotides poly ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00172a018
更新日期:1994-02-15 00:00:00
abstract::We identified amino acid residues important for activity of sod2, the Na+/H+ antiporter of Schizosaccharomyces pombe. We mutated all eight His residues of sod2 into Arg. Only His367-->Arg affected function and resulted in complete inability of sod2 to allow growth of S. pombe in LiCl-containing medium. Mutant S. pombe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9801457
更新日期:1998-06-09 00:00:00
abstract::ATP, its nonhydrolyzable analogue, AMP-PNP, and albumin were found to promote the dissociation of rhodopsin kinase from rod outer segments (ROS) containing photoactivated-rhodopsin (Rho*). These features were embodied in a protocol for the recovery of rhodopsin kinase from incubations containing ROS which had been sub...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952480q
更新日期:1996-05-14 00:00:00
abstract::Perturbations within the transactivation and carboxy-terminal domains of HIV-1 Rev were examined for effects on Rev responsive element (RRE) binding activities in vitro and biological activity in vivo. Binding affinities, specificities, and multimerization of the transactivation mutants M10 and Rev/Rex M10-16 on the R...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00085a028
更新日期:1993-08-31 00:00:00
abstract::Quantum tunneling and protein dynamics have emerged as important components of enzyme function. This review focuses on soybean lipoxygenase-1, to illustrate how the properties of enzymatic C-H bond activation link protein motions to the fundamental bond making-breaking processes. ...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi301504m
更新日期:2013-03-26 00:00:00
abstract::The RcnR metalloregulator represses the transcription of the Co(II) and Ni(II) exporter, RcnAB. Previous studies have shown that Co(II) and Ni(II) bind to RcnR in six-coordinate sites, resulting in derepression. Here, the roles of His60, His64, and His67 in specific metal recognition are examined. His60 and His64 corr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300886q
更新日期:2013-01-08 00:00:00
abstract::The effects of nonylphenol and 3,5-dibutyl-4-hydroxytoluene (BHT) on the activity of the (Ca2(+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum have been studied. At high concentrations, both inhibit the ATPase activity of the ATPase either in native lipid or in bilayers of dioleoylphosphatidylcholine but, at ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00464a028
更新日期:1990-03-27 00:00:00
abstract::The combination of UV/visible/near-IR variable-temperature magnetic circular dichroism (VTMCD) and EPR spectroscopies has been used to investigate the spin states and axial ligation of the heme group in oxidized, reduced, and CO-bound reduced forms of the Rhodospirillum rubrum CO oxidation transcriptional activator pr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991303c
更新日期:1999-09-28 00:00:00
abstract::Electron paramagnetic resonance (EPR) imaging utilizing stable nitroxyl radicals is a promising technique for measuring free radical distribution, metabolism, and tissue oxygenation in organs and tissues [Kuppusamy, P., Chzhan, M., Vij, K., Shteynbuk, M., Lefer, D. J., Giannella, E., & Zweier, J. L. (1994) Proc. Natl....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952857s
更新日期:1996-06-04 00:00:00
abstract::The folding of the two-domain bacterial chaperone PapD has been studied to develop an understanding of the relationship between individual domain folding and the formation of domain-domain interactions. PapD contains six phenylalanine residues, four in the N-terminal domain and two in the C-terminal domain. To examine...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048614u
更新日期:2004-11-02 00:00:00
abstract::In Gram-negative bacteria, the first step of lipid A biosynthesis is catalyzed by UDP-N-acetylglucosamine acyltransferase (LpxA) through the transfer of a R-3-hydroxyacyl chain from the acyl carrier protein (ACP) to the 3-hydroxyl group of UDP-GlcNAc. Previous studies suggest that LpxA is a critical determinant of the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00720
更新日期:2015-09-29 00:00:00
abstract::The first committed step of guanine nucleotide biosynthesis is the oxidation of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) catalyzed by IMP dehydrogenase. The reaction involves the reduction of NAD(+) with the formation of a covalent enzyme intermediate (E-XMP). Hydrolysis of E-XMP requires th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048342v
更新日期:2005-09-06 00:00:00
abstract::Sarcosine oxidase from Corynebacterium sp. P-1 binds 2 mol of tetrahydrofolate/mol of enzyme (KD = 8.8 microM). The same stoichiometry is observed with tetrahydropteroyltetraglutamate (KD = 15.4 microM). Binding is also observed with pteroyltetraglutamate and with 5-formyltetrahydrofolate. In the case of the pteroylmo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00397a029
更新日期:1987-11-17 00:00:00
abstract::Blastochloris tepida is a newly described thermophilic purple bacterium containing bacteriochlorophyll b. Using purified light-harvesting 1 reaction center (LH1-RC) core complexes from Blc. tepida, we compared the biochemical, spectroscopic, and thermal denaturation properties of these complexes with those of its meso...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00192
更新日期:2020-06-30 00:00:00
abstract::Engineering proteins to withstand a broad range of conditions continues to be a coveted objective, holding the potential to advance biomedicine, industry, and our understanding of disease. One way of achieving this goal lies in elucidating the underlying interactions that define protein stability. It has been shown th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0600143
更新日期:2006-03-07 00:00:00
abstract::IIIGlc is an 18.1-kDa signal-transducing phosphocarrier protein of the phosphoenolpyruvate:glycose phosphotransferase system (PTS) of Escherichia coli. Virtually complete (98%) backbone 1H, 15N, and 13C nuclear magnetic resonance (NMR) signal assignments were determined by using a battery of triple-resonance three-dim...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00105a032
更新日期:1991-10-15 00:00:00
abstract::Recent evidence adds support to a traditional concept according to which the eukaryotic nucleus is organized into functional domains by scaffold or matrix attachment regions (S/MARs). These regions have previously been predicted to have a high potential for stress-induced duplex destabilization (SIDD). Here we report ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026496+
更新日期:2003-01-14 00:00:00
abstract::The effects of pH on the kinetics of association and dissociation of chymotrypsin and the dipeptidyl trifluoromethyl ketone (TFK) N-acetyl-L-leucyl-L-phenylalanyltrifluoromethane (1) were examined through the pH range 4-9.5. The pH dependence of the association rate (kon) is similar to that of kcat/Km for ester and pe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00449a017
更新日期:1989-11-14 00:00:00