当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Transmembrane topology of mammalian planar cell polarity protein Vangl1.

Transmembrane topology of mammalian planar cell polarity protein Vangl1.

Abstract：

:Vangl1 and Vangl2 are membrane proteins that play an important role in neurogenesis, and Vangl1/Vangl2 mutations cause neural tube defects in mice and humans. At the cellular level, Vangl proteins regulate the establishment of planar cell polarity (PCP), a process requiring membrane assembly of asymmetrically distributed multiprotein complexes that transmit polarity information to neighboring cells. The membrane topology of Vangl proteins and the protein segments required for structural and functional aspects of multiprotein membrane PCP complexes is unknown. We have used epitope tagging and immunofluorescence to establish the secondary structure of Vangl proteins, including the number, position, and polarity of transmembrane domains. Antigenic hemagglutinin A (HA) peptides (YYDVPDYS) were inserted in predicted intra- or extracellular loops of Vangl1 at positions 18, 64, 139, 178, 213, and 314, and individual mutant variants were stably expressed at the membrane of MDCK polarized cells. The membrane topology of the exofacial HA tag was determined by a combination of immunofluorescence in intact (extracellular epitopes) and permeabilized (intracellular epitopes) cells and by surface labeling. Results indicate that Vangl proteins have a four-transmembrane domain structure with the N-terminal portion (HA18 and HA64) and the large C-terminal portion (HA314) of the protein being intracellular. Topology mapping and hydropathy profiling show that the loop delineated by TMD1-2 (HA139) and TMD3-4 (HA213) is extracellular while the segment separating predicted TMD2-3 (HA178) is intracellular. This secondary structure reveals a compact membrane-associated portion with very short predicted extra- and intracellular loops, while the protein harbors a large intracellular domain.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Iliescu A,Gravel M,Horth C,Apuzzo S,Gros P

doi

10.1021/bi101767a

subject

Has Abstract

pub_date

2011-03-29 00:00:00

pages

2274-82

issue

12

eissn

0006-2960

issn

1520-4995

journal_volume

50

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Important role of the proline residue in the signal sequence that directs the secretion of human lysozyme in Saccharomyces cerevisiae.

    abstract：:To elucidate the role of the proline residue in the engineered signal sequence that directs the secretion of human lysozyme in Saccharomyces cerevisiae, we have remodeled an idealized signal sequence L8 = Met-Arg-(Leu)8-Pro-Leu-Ala-Ala-Leu-Gly [Yamamoto, Y., Taniyama, Y., Kikuchi, M., & Ikehara, M. (1987) Biochem. Bio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00432a054

    authors： Yamamoto Y,Taniyama Y,Kikuchi M

    更新日期：1989-03-21 00:00:00

  • Pharmacological and functional comparison of the polo-like kinase family: insight into inhibitor and substrate specificity.

    abstract：:PLK1 (polo-like kinase 1) is a key mitotic kinase and a therapeutic target in the treatment of proliferative diseases. Here we investigate the relative substrate specificity and pharmacological relatedness of PLK1, -2, -3, and -4 that together comprise a conserved family of Ser/Thr kinases (PLK family). We report cons...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7008745

    authors： Johnson EF,Stewart KD,Woods KW,Giranda VL,Luo Y

    更新日期：2007-08-21 00:00:00

  • An isoleucine to leucine mutation that switches the cofactor requirement of the EcoRV restriction endonuclease from magnesium to manganese.

    abstract：:The EcoRV restriction endonuclease cleaves DNA at its recognition sequence more readily with Mg2+ as the cofactor than with Mn2+ but, at noncognate sequences that differ from the EcoRV site by one base pair, Mn2+ gives higher rates than Mg2+. A mutant of EcoRV, in which an isoleucine near the active site was replaced ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9523926

    authors： Vipond IB,Moon BJ,Halford SE

    更新日期：1996-02-13 00:00:00

  • Conformational changes in human hepatitis C virus NS3 protease upon binding of product-based inhibitors.

    abstract：:One of the most promising approaches to anti-hepatitis C virus drug discovery is the development of inhibitors of the virally encoded protease NS3. This chymotrypsin-like serine protease is essential for the maturation of the viral polyprotein, and processing requires complex formation between NS3 and its cofactor NS4...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991220w

    authors： Bianchi E,Orrù S,Dal Piaz F,Ingenito R,Casbarra A,Biasiol G,Koch U,Pucci P,Pessi A

    更新日期：1999-10-19 00:00:00

  • Prevention of Clostridium sordellii lethal toxin-induced apoptotic cell death by tauroursodeoxycholic acid.

    abstract：:Virulent strains of Clostridium sordellii cause gangrenous myonecrosis in humans. The released lethal toxin (TcsL) and hemorrhagic toxin (TcsH) are regarded as the major virulence factors. TcsL inactivates low molecular weight GTP-binding proteins of the Rho/Ras subfamilies by monoglucosylation. In cultured cell lines...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900964c

    authors： Schulz F,Just I,Genth H

    更新日期：2009-09-29 00:00:00

  • MutY DNA glycosylase: base release and intermediate complex formation.

    abstract：:MutY protein, a DNA glycosylase found in Escherichia coli, recognizes dA:dG, dA:8-oxodG, and dA:dC mismatches in duplex DNA, excising the adenine moiety. We have investigated the mechanism of action of MutY, addressing several points of disagreement raised by previous studies of this enzyme. MutY forms a covalent inte...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981066y

    authors： Zharkov DO,Grollman AP

    更新日期：1998-09-08 00:00:00

  • Molecular cloning of testicular 20 alpha-hydroxysteroid dehydrogenase: identity with aldose reductase.

    abstract：:Complementary DNA (cDNA) clones encoding bovine testicular 20 alpha-hydroxysteroid dehydrogenase (20 alpha-HSD) have been isolated from a bovine testicular lambda gt11 library using polyclonal antibodies against 20 alpha-HSD and DNA probe hybridization. Nucleotide sequencing of three independently isolated clones was ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00057a003

    authors： Warren JC,Murdock GL,Ma Y,Goodman SR,Zimmer WE

    更新日期：1993-02-16 00:00:00

  • Nitrogen-15 NMR spectroscopy of the catalytic-triad histidine of a serine protease in peptide boronic acid inhibitor complexes.

    abstract：:15N NMR spectroscopy was used to examine the active-site histidyl residue of alpha-lytic protease in peptide boronic acid inhibitor complexes. Two distinct types of complexes were observed: (1) Boronic acids that are analogues of substrates form complexes in which the active-site imidazole ring is protonated and both ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00420a018

    authors： Bachovchin WW,Wong WY,Farr-Jones S,Shenvi AB,Kettner CA

    更新日期：1988-10-04 00:00:00

  • Functional groups at the catalytic site of BF1 adenosinetriphosphatase from Escherichia coli.

    abstract：:The rates of inactivation of BF1 adenosinetriphosphatase (BF1-ATPase) from Escherichia coli by 7-chloro-4-nitro-2,1,3-benzoxadiazole, 1-fluoro-2,4-dinitrobenzene, 2,4,6-trinitrobenzenesulfonate, phenylglyoxal, and N,N'-dicyclohexylcarbodiimide have been measured in the presence and absence of various concentrations of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00531a011

    authors： Ting LP,Wang JH

    更新日期：1982-01-19 00:00:00

  • In vitro reconstitution of the first steps of anatoxin-a biosynthesis in Oscillatoria PCC 6506: from free L-proline to acyl carrier protein bound dehydroproline.

    abstract：:Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins in cyanobacteria and proposed a biosynthetic scheme starting from L-proline and involving three polyketide synthases for which the starte...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9018785

    authors： Méjean A,Mann S,Vassiliadis G,Lombard B,Loew D,Ploux O

    更新日期：2010-01-12 00:00:00

  • Role of conserved active site residues in catalysis by phospholipase B1 from Cryptococcus neoformans.

    abstract：:Phospholipase B1 (PLB1), secreted by the pathogenic yeast Cryptococcus neoformans, has an established role in virulence. Although the mechanism of its phospholipase B, lysophospholipase, and lysophospholipase transacylase activities is unknown, it possesses lipase, subtilisin protease aspartate, and phospholipase moti...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7009508

    authors： Jones PM,Turner KM,Djordjevic JT,Sorrell TC,Wright LC,George AM

    更新日期：2007-09-04 00:00:00

  • Distinct reactions catalyzed by bacterial and yeast trans-aconitate methyltransferases.

    abstract：:The trans-aconitate methyltransferase from the bacterium Escherichia coli catalyzes the monomethyl esterification of trans-aconitate and related compounds. Using two-dimensional (1)H/(13)C nuclear magnetic resonance spectroscopy, we show that the methylation is specific to one of the three carboxyl groups and further ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0022902

    authors： Cai H,Strouse J,Dumlao D,Jung ME,Clarke S

    更新日期：2001-02-20 00:00:00

  • 1H nuclear magnetic resonance double resonance study of oxytocin in aqueous solution.

    abstract：:Peptide NH resonances in the 250 MHZ 1H nuclear magnetic resonance (NMR) spectrum of oxytocin in H2O were assigned to specific amino acid residues by the "underwater decoupling" technique (i.e., decoupling from corresponding CalphaH resonances, which are buried beneath the intense water peak). These experiments confir...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00650a025

    authors： Glickson JD,Rowan R,Pitner TP,Dadok J,Bothner-By AA,Walter R

    更新日期：1976-03-09 00:00:00

  • The 2.15 A crystal structure of Mycobacterium tuberculosis chorismate mutase reveals an unexpected gene duplication and suggests a role in host-pathogen interactions.

    abstract：:Chorismate mutase catalyzes the first committed step toward the biosynthesis of the aromatic amino acids, phenylalanine and tyrosine. While this biosynthetic pathway exists exclusively in the cell cytoplasm, the Mycobacterium tuberculosis enzyme has been shown to be secreted into the extracellular medium. The secretor...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0606445

    authors： Qamra R,Prakash P,Aruna B,Hasnain SE,Mande SC

    更新日期：2006-06-13 00:00:00

  • Circadian genomics reveal a role for post-transcriptional regulation in mammals.

    abstract：:To maintain daily cycles, the circadian clock must tightly regulate the rhythms of thousands of mRNAs and proteins with the correct period, phase, and amplitude to ultimately drive the wide range of rhythmic biological processes. Recent genomic approaches have revolutionized our view of circadian gene expression and h...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi500707c

    authors： Kojima S,Green CB

    更新日期：2015-01-20 00:00:00

  • Identification of amino acids important for binding of Clostridium perfringens epsilon toxin to host cells and to HAVCR1.

    abstract：:Clostridium perfringens epsilon toxin belongs to the aerolysin-like family of pore-forming toxins and is one of the most potent bacterial toxins known. The epsilon toxin causes fatal enterotoxemia in sheep, goats, and possibly humans. Evidence indicates that the toxin binds to protein receptors including hepatitis A v...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300690a

    authors： Ivie SE,McClain MS

    更新日期：2012-09-25 00:00:00

  • Interactions of poly(N epsilon, N epsilon, N epsilon-trimethyllysine) and poly(N delta, N delta, N delta-trimethylornithine) with polynucleotides: salt dissociation and thermal denaturation.

    abstract：:The interaction of poly(N epsilon, N epsilon, N epsilon-trimethyl-L-lysine) ([Lys(Me3)]n) and poly(N delta, N delta, N delta-trimethyl-L-ornithine) ([Orn(Me3)]n) with polynucleotides was studied by thermal denaturation, viscosity, and dissociation by salt. The methylated polymers decrease the viscosity of DNA in propo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00555a020

    authors： Granados EN,Bello J

    更新日期：1980-07-08 00:00:00

  • G Protein betagamma dimer formation: Gbeta and Ggamma differentially determine efficiency of in vitro dimer formation.

    abstract：:The Gbeta and Ggamma subunit of the heterotrimeric G proteins form a functional dimer that is stable once assembled in vivo or in vitro. The requirements, mechanism, and specificity of dimer formation are still incompletely understood, but represent important biochemical processes involved in the specificity of cellul...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0504254

    authors： Dingus J,Wells CA,Campbell L,Cleator JH,Robinson K,Hildebrandt JD

    更新日期：2005-09-06 00:00:00

  • N-terminal disordered domain of Saccharomyces cerevisiae Hop1 protein is dispensable for DNA binding, bridging, and synapsis of double-stranded DNA molecules but is necessary for spore formation.

    abstract：:The cytological architecture of the synaptonemal complex (SC), a meiosis-specific proteinaceous structure, is evolutionarily conserved among eukaryotes. However, little is known about the biochemical properties of SC components or the mechanisms underlying their roles in meiotic chromosome synapsis and recombination. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi4005528

    authors： Khan K,Madhavan TP,Kshirsagar R,Boosi KN,Sadhale P,Muniyappa K

    更新日期：2013-08-06 00:00:00

  • Catalytically active cross-species heterodimers of thymidylate synthase.

    abstract：:Thymidylate synthase (TS) is a highly conserved homodimeric enzyme with two active sites, each of which contains amino acid residues from both subunits. We show that the conservation at the subunit interface between Escherichia coli TS and Lactobacillus casei TS is sufficient to permit the formation of a cross-species...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00090a002

    authors： Greene PJ,Maley F,Pedersen-Lane J,Santi DV

    更新日期：1993-10-05 00:00:00

  • Electrostatic Control of Isoform Selective Inhibitor Binding in Nitric Oxide Synthase.

    abstract：:Development of potent and isoform selective nitric oxide synthase (NOS) inhibitors is challenging because of the structural similarity in the heme active sites. One amino acid difference between NOS isoforms, Asp597 in rat neuronal NOS (nNOS) versus Asn368 in bovine endothelial NOS (eNOS), has been identified as the s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00261

    authors： Li H,Wang HY,Kang S,Silverman RB,Poulos TL

    更新日期：2016-07-05 00:00:00

  • Reduction precedes cytidylyl transfer without substrate channeling in distinct active sites of the bifunctional CDP-ribitol synthase from Haemophilus influenzae.

    abstract：:CDP-ribitol synthase is a bifunctional reductase and cytidylyltransferase that catalyzes the transformation of D-ribulose 5-phosphate, NADPH, and CTP to CDP-ribitol, a repeating unit present in the virulence-associated polysaccharide capsules of Haemophilus influenzae types a and b [Follens, A., et al. (1999) J. Bacte...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002745n

    authors： Zolli M,Kobric DJ,Brown ED

    更新日期：2001-04-24 00:00:00

  • Divergence of structure and function in the haloacid dehalogenase enzyme superfamily: Bacteroides thetaiotaomicron BT2127 is an inorganic pyrophosphatase.

    abstract：:The explosion of protein sequence information requires that current strategies for function assignment evolve to complement experimental approaches with computationally based function prediction. This necessitates the development of strategies based on the identification of sequence markers in the form of specificity ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201181q

    authors： Huang H,Patskovsky Y,Toro R,Farelli JD,Pandya C,Almo SC,Allen KN,Dunaway-Mariano D

    更新日期：2011-10-18 00:00:00

  • Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.

    abstract：:Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00005a017

    authors： Driessen AJ,van den Hooven HW,Kuiper W,van de Kamp M,Sahl HG,Konings RN,Konings WN

    更新日期：1995-02-07 00:00:00

  • Effects of recombinant protein expression on green fluorescent protein diffusion in Escherichia coli.

    abstract：:Fluorescence recovery after photobleaching was used to measure the diffusion coefficient of green fluorescent protein (GFP, 27 kDa) in Escherichia coli in the presence or absence of four coexpressed proteins: cytoplasmic maltose binding protein (42 kDa), tau-40 (45 kDa), alpha-synuclein (14 kDa), or calmodulin (17 kDa...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9004107

    authors： Slade KM,Baker R,Chua M,Thompson NL,Pielak GJ

    更新日期：2009-06-16 00:00:00

  • A protein arginine N-methyltransferase 1 (PRMT1) and 2 heteromeric interaction increases PRMT1 enzymatic activity.

    abstract：:Protein arginine N-methyltransferases (PRMTs) act in signaling pathways and gene expression by methylating arginine residues within target proteins. PRMT1 is responsible for most cellular arginine methylation activity and can work independently or in collaboration with other PRMTs. In this study, we demonstrate a dire...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200644c

    authors： Pak ML,Lakowski TM,Thomas D,Vhuiyan MI,Hüsecken K,Frankel A

    更新日期：2011-09-27 00:00:00

  • Mutations affecting the activity of toxic shock syndrome toxin-1.

    abstract：:Toxic shock syndrome toxin-1 (TSST-1), the potent staphylococcal exoprotein linked to most cases of the toxic shock syndrome, is a V beta-restricted T-cell mitogen (a so-called "superantigen"). TSST-ovine (TSST-O) is a natural variant of TSST-1, and is produced by certain ovine mastitis-associated strains of Staphyloc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00209a016

    authors： Deresiewicz RL,Woo J,Chan M,Finberg RW,Kasper DL

    更新日期：1994-11-01 00:00:00

  • Binding of hemoglobin to red cell membranes with eosin-5-maleimide-labeled band 3: analysis of centrifugation and fluorescence data.

    abstract：:We have studied the binding of hemoglobin to the red cell membrane by centrifugation and fluorescence methods. The intact red cell was labeled with eosin-5-maleimide (EM), which specifically reacts with lysine 430 of band 3. Even though this residue is not part of the cytoplasmic domain of band 3 (cdb3) associated wit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi012007e

    authors： Demehin AA,Abugo OO,Jayakumar R,Lakowicz JR,Rifkind JM

    更新日期：2002-07-09 00:00:00

  • Antiapoptotic signaling by the insulin receptor in Chinese hamster ovary cells.

    abstract：:We have sought to determine whether insulin can promote cell survival and protect Chinese hamster ovary (CHO) cells from apoptosis induced by serum starvation. Low concentrations of insulin were antiapoptotic for cells overexpressing wild-type insulin receptors but not in cells transfected with kinase-defective insuli...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9805947

    authors： Lee-Kwon W,Park D,Baskar PV,Kole S,Bernier M

    更新日期：1998-11-10 00:00:00

  • Isolation and characterization of an anticoagulant protein homologous to botrocetin from the venom of Bothrops jararaca.

    abstract：:We previously isolated a unique anticoagulant protein named IX/X-bp (factor IX/factor X-binding protein) from the venom of the habu snake Trimeresurus flavoviridis. We recently determined its primary structure and found that this protein had a structure homologous to the carbohydrate-recognition domains of C-type lect...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00078a012

    authors： Sekiya F,Atoda H,Morita T

    更新日期：1993-07-13 00:00:00