Oxy intermediates of homoprotocatechuate 2,3-dioxygenase: facile electron transfer between substrates.

abstract：:Amyloid formation by immunoglobulin light chain (LC) proteins is associated with amyloid light chain (AL) amyloidosis. Destabilization of the native state of the variable domain of the LC (VL) is known to be one of the critical factors in promoting the formation of amyloid fibrils. However, determining the key residue...

journal_title：Biochemistry

authors： Kobayashi Y,Tsutsumi H,Abe T,Ikeda K,Tashiro Y,Unzai S,Kamikubo H,Kataoka M,Hiroaki H,Hamada D

更新日期：2014-08-12 00:00:00

abstract：:ROS-GC represents a membrane guanylate cyclase subfamily whose distinctive feature is that it transduces diverse intracellularly generated Ca(2+) signals into the production of the second messenger cyclic GMP. An intriguing feature of the first subfamily member, ROS-GC1, is that it is both stimulated and inhibited by ...

journal_title：Biochemistry

authors： Kumar VD,Vijay-Kumar S,Krishnan A,Duda T,Sharma RK

更新日期：1999-09-28 00:00:00

abstract：:The substrate specificity of the human enzyme glutathione reductase was changed from its natural substrate glutathione to trypanothione [N1,N8-bis(glutathionyl)spermidine] by site-directed mutagenesis of two residues. The glutathione analogue, trypanothione, is the natural substrate for trypanothione reductase, an enz...

journal_title：Biochemistry

authors： Bradley M,Bücheler US,Walsh CT

更新日期：1991-06-25 00:00:00

abstract：:Superoxide reductase (SOR) is an Fe protein that catalyzes the reduction of superoxide to give H(2)O(2). Recently, the mutation of the Glu47 residue into alanine (E47A) in the active site of SOR from Desulfoarculus baarsii has allowed the stabilization of an iron-peroxo species when quickly reacted with H(2)O(2) [Math...

journal_title：Biochemistry

authors： Horner O,Mouesca JM,Oddou JL,Jeandey C,Nivière V,Mattioli TA,Mathé C,Fontecave M,Maldivi P,Bonville P,Halfen JA,Latour JM

更新日期：2004-07-13 00:00:00

abstract：:Seipin is a transmembrane protein that resides in the endoplasmic reticulum and concentrates at junctions between the ER and cytosolic lipid droplets. Mutations in the human seipin gene, including the missense mutation A212P, lead to congenital generalized lipodystrophy (CGL), characterized by the lack of normal adipo...

journal_title：Biochemistry

authors： Binns D,Lee S,Hilton CL,Jiang QX,Goodman JM

更新日期：2010-12-21 00:00:00

abstract：:A cell-free system for synthesizing protein from wheat germ was used to translate the messenger RNA extracted from 16-day embryonic chick calvaria. A part of the product had properties similar to collagenous peptides and served as a substrate for prolyl hydroxylase, an enzyme specific for collagen. The level of potass...

journal_title：Biochemistry

authors： Benveniste K,Wilczek J,Ruggieri A,Stern R

更新日期：1976-02-24 00:00:00

abstract：:There is no complete understanding of how serine protease inhibitors of the serpin family inhibit their target enzymes. Structural and biochemical studies have suggested that serpins utilize a mechanism that is distinct from the standard mechanism of inhibition proposed for most small protein protease inhibitors. Prot...

journal_title：Biochemistry

authors： Plotnick MI,Mayne L,Schechter NM,Rubin H

更新日期：1996-06-11 00:00:00

abstract：:8-OxoGua (8-oxo-7,8-dihydroguanine) is produced in nucleic acids as well as in nucleotide pools of cells, by reactive oxygen species normally formed during cellular metabolic processes. MutT protein of Escherichia coli specifically degrades 8-oxoGua-containing deoxyribo- and ribonucleoside triphosphates to correspondi...

journal_title：Biochemistry

authors： Ito R,Hayakawa H,Sekiguchi M,Ishibashi T

更新日期：2005-05-03 00:00:00

abstract：:A major biochemical goal is the ability to mimic nature in engineering highly specific protein-protein interactions (PPIs). We previously devised a computational interactome screen to identify eight peptides that form four heterospecific dimers despite 32 potential off-targets. To expand the speed and utility of our a...

journal_title：Biochemistry

authors： Crooks RO,Lathbridge A,Panek AS,Mason JM

更新日期：2017-03-21 00:00:00

abstract：:The interactions of myelin basic protein and peptides derived from it with detergent micelles of lysophosphatidylglycerol, lysophosphatidylserine, palmitoyllysophosphatidic acid, and sodium lauryl sulfate, and with mixed micelles of the neutral detergent dodecylphosphocholine and the negatively charged detergent palmi...

journal_title：Biochemistry

authors： Mendz GL,Brown LR,Martenson RE

更新日期：1990-03-06 00:00:00

abstract：:A metabolite of vitamin D has been isolated in pure form from incubation of 25-hydroxyvitamin D3 with embryonic chick calvarial cells that had been grown on Cytodex 1 microcarrier beads. The isolation involved dichloromethane extraction of the cells and incubation medium, followed by Sephadex LH-20 column chromatograp...

journal_title：Biochemistry

authors： Turner RT,Howard GA,Puzas JE,Baylink DJ,Knapp DR

更新日期：1983-03-01 00:00:00

abstract：:Fluorescence lifetimes and fluorescence anisotropy decays of the voltage-sensitive styryl-pyridinium dye RH421 have been measured in the presence of dimyristoylphosphatidylcholine vesicles, the water soluble enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco), and Na+,K(+)-ATPase-containing membrane fragm...

journal_title：Biochemistry

authors： Visser NV,van Hoek A,Visser AJ,Frank J,Apell HJ,Clarke RJ

更新日期：1995-09-19 00:00:00

abstract：:With excitation at 406.7 nm, we have observed the resonance Raman enhancement of the bound v(N-O) stretch at approximately 1623 cm-1 in nitrosylhemoglobin A and nitrosylmyoglobin, indicating the existence of a charge-transfer transition underlying the strong Soret band. The v(Fe-NO) stretch at 551 cm-1 has also been d...

journal_title：Biochemistry

authors： Tsubaki M,Yu NT

更新日期：1982-03-16 00:00:00

abstract：:The stereochemical problem involving a pro-pro-prochiral phosphorus center, the hydrolysis of adenosine 5'-monophosphate to adenosine and inorganic phosphate catalyzed by the venom 5'-nucleotidase, has been studied by use of chiral [16O, 17O, 18O]thiophosphates (Psi). (Rp)- and (Sp)-[alpha-18O1]Adenosine 5'-thiophosph...

journal_title：Biochemistry

authors： Tsai MD

更新日期：1980-11-11 00:00:00

abstract：:G-protein-coupled receptors (GPCRs) have evolved as highly specialized cellular machinery that can dictate biological outcomes in response to diverse stimuli. Specifically, they induce multiple pathway responses upon structural perturbations induced at local protein sites. GPCRs utilize a concurrent strategy involving...

journal_title：Biochemistry

authors： Jatana N,Aswin SK,Rathore S,Thukral L

更新日期：2020-01-28 00:00:00

abstract：:We obtained a novel carbohydrate-binding peptide having a helix-loop-helix scaffold from a random peptide library. The helix-loop-helix peptide library randomized at five amino acid residues was displayed on the major coat protein of a filamentous phage. Affinity selection with a ganglioside, Galbeta1-3GalNAcbeta1-4(N...

journal_title：Biochemistry

authors： Matsubara T,Iida M,Tsumuraya T,Fujii I,Sato T

更新日期：2008-07-01 00:00:00

abstract：:S-Protein/vitronectin is a serum glycoprotein that inhibits the lytic activity of the membrane attack complex of complement, i.e., of the complex including the proteins C5b, C6, C7, C8, and C9n. We show that intact S-protein/vitronectin or its cyanogen bromide generated fragments also inhibit the hemolysis mediated by...

journal_title：Biochemistry

authors： Tschopp J,Masson D,Schäfer S,Peitsch M,Preissner KT

更新日期：1988-05-31 00:00:00

abstract：:VanX, one of the five proteins required for the vancomycin-resistant phenotype in clinically pathogenic Enterococci, is a zinc-containing d-Ala-d-Ala dipeptidase. To identify potential zinc ligands and begin defining the active site residues, we have mutated the 2 cysteine, 5 histidine, and 4 of the 28 aspartate and g...

journal_title：Biochemistry

authors： McCafferty DG,Lessard IA,Walsh CT

更新日期：1997-08-26 00:00:00

abstract：:The gene product 61 primase protein from bacteriophage T4 was expressed as an intein fusion and purified to homogeneity. The primase binds one zinc ion, which is coordinated by four cysteine residues to form a zinc ribbon motif. Factors that influence the rate of priming were investigated, and a physiologically releva...

journal_title：Biochemistry

authors： Valentine AM,Ishmael FT,Shier VK,Benkovic SJ

更新日期：2001-12-18 00:00:00

abstract：:Nhp6A is an abundant non-histone chromatin-associated protein in Saccharomyces cerevisiae that contains a minor groove DNA binding motif called the HMG box. In this report, we show that Nhp6Ap binds to cisplatin intrastrand cross-links on duplex DNA with a 40-fold greater affinity than to unmodified DNA with the same ...

journal_title：Biochemistry

authors： Wong B,Masse JE,Yen YM,Giannikopoulos P,Feigon J,Johnson RC

更新日期：2002-04-30 00:00:00

abstract：:Iron K-edge X-ray spectroscopy (XANES and EXAFS) was used to study iron coordination in frozen solutions of soybean lipoxygenase-1 (SLO). The intensity of the 1s-->3d pre-edge transition of native iron(II) lipoxygenase is greater than what was found for six-coordinate high-spin iron(II) model complexes, but comparable...

journal_title：Biochemistry

authors： Scarrow RC,Trimitsis MG,Buck CP,Grove GN,Cowling RA,Nelson MJ

更新日期：1994-12-20 00:00:00

abstract：:A new series of peptide inhibitors of human Factor VIIa (FVIIa) has been identified and affinity matured from naive and partially randomized peptide phage libraries selected against the immobilized tissue factor x Factor VIIa (TF x FVIIa) complex. These "A-series" peptides contain a single disulfide bond and a 13-resi...

journal_title：Biochemistry

authors： Dennis MS,Roberge M,Quan C,Lazarus RA

更新日期：2001-08-14 00:00:00

abstract：:The effect of Mg2+ on the disposition of myosin cross-bridges was studied on myofibrils and synthetic myosin and rod filaments by employing chymotryptic digestion and chemical cross-linking methods. In the presence of low Mg2+ concentrations (0.1 mM), the proteolytic susceptibility at the heavy meromyosin/light meromy...

journal_title：Biochemistry

authors： Reisler E,Liu J,Cheung P

更新日期：1983-10-11 00:00:00

abstract：:The transporter associated with antigen processing (TAP) is a prototype of an asymmetric ATP-binding cassette (ABC) transporter, which uses ATP binding and hydrolysis to translocate peptides from the cytosol to the lumen of the endoplasmic reticulum (ER). Here, we review molecular details of peptide binding and ATP bi...

journal_title：Biochemistry

authors： Hinz A,Tampé R

更新日期：2012-06-26 00:00:00

abstract：:RecA-catalyzed DNA recombination is initiated by a mandatory, high-energy form of DNA in RecA-nucleoprotein filaments, where bases are highly unstacked and the backbone is highly unwound. Interestingly, only the energetics consequent to adenosine triphosphate (ATP) binding, rather than its hydrolysis, seems sufficient...

journal_title：Biochemistry

authors： Ramreddy T,Sen S,Rao BJ,Krishnamoorthy G

更新日期：2003-10-21 00:00:00

abstract：:We have used a fluorescent analogue of ATP, mantATP [2'(3')-O-(N-methylanthraniloyl)-adenosine 5'-triphosphate; Hiratsuka T. (1983) Biochim. Biophys. Acta 742, 496-508], and made a detailed kinetic study of the interaction of mantATP and mantADP with S1 and acto-S1. We have shown that these analogues behave like ATP a...

journal_title：Biochemistry

authors： Woodward SK,Eccleston JF,Geeves MA

更新日期：1991-01-15 00:00:00

abstract：:Propeptide processing occurs in specific compartments of the secretory pathway, but how these processing-competent organelles are generated from their processing-incompetent precursor compartments is unknown. To dissect the process biochemically, we have developed a novel cell-free system reconstituting the production...

journal_title：Biochemistry

authors： Andresen JM,Moore HP

更新日期：2001-10-30 00:00:00

abstract：:The DNA binding domain of the yeast transcription factor Mbp1 is a winged helix-turn-helix structure, with an extended DNA binding site involving C-terminal "tail" residues. The thermodynamics of the interaction of the DNA binding domain with its target DNA sequence have been determined using fluorescence anisotropy a...

journal_title：Biochemistry

authors： Deleeuw L,Tchernatynskaia AV,Lane AN

更新日期：2008-06-17 00:00:00

abstract：:hOgg1 and FPG are the primary DNA repair enzymes responsible for removing the major guanine (G) oxidative product, namely, 7,8-dihydro-8-oxoguanine (OG), in humans and bacteria, respectively. While natural G adopts the anti conformation and forms a Watson-Crick pair with cytosine (C), OG can also adopt the syn conform...

journal_title：Biochemistry

authors： Sowlati-Hashjin S,Wetmore SD

更新日期：2018-02-20 00:00:00

abstract：:The biosynthesis of ansamycin antibiotics, including rifamycin B, involves the synthesis of an aromatic precursor, 3-amino-5-hydroxybenzoic acid (AHBA), which serves as starter for the assembly of the antibiotics' polyketide backbone. The terminal enzyme of AHBA formation, AHBA synthase, is a dimeric, pyridoxal 5'-pho...

journal_title：Biochemistry

authors： Eads JC,Beeby M,Scapin G,Yu TW,Floss HG

更新日期：1999-08-03 00:00:00