Interactions of myelin basic protein with mixed dodecylphosphocholine/palmitoyllysophosphatidic acid micelles.

abstract：:The class C serine beta-lactamase of Enterobacter cloacae P99 was inhibited by a series of aryl methylphosphonate monoester monoanions. The effectiveness of these inhibitors was promoted by an acylamido substituent on the methyl group and a good leaving group at phosphorus. The former preference suggests that noncoval...

journal_title：Biochemistry

authors： Rahil J,Pratt RF

更新日期：1992-06-30 00:00:00

abstract：:l-Aspartate oxidase, encoded by the nadB gene, is the first enzyme in the de novo synthesis of NAD+ in bacteria. This FAD-dependent enzyme catalyzes the oxidation of l-aspartate to generate iminoaspartate and reduced flavin. Distinct from most amino acid oxidases, it can use either molecular oxygen or fumarate to reox...

journal_title：Biochemistry

authors： Chow C,Hegde S,Blanchard JS

更新日期：2017-08-08 00:00:00

abstract：:Escherichia coli cAMP receptor protein (CRP) controls more than 20 genes. There are significant differences in the promoter regions in these genes. Thus, an elucidation of the mechanism of CRP action requires knowledge about the mode of selectivity in these promoters. An earlier study [Heyduk, T., & Lee, J. C. (1990) ...

journal_title：Biochemistry

authors： Pyles EA,Lee JC

更新日期：1996-01-30 00:00:00

abstract：:The effects of several divalent cations, various polybasic amines, and lysozyme on the rate of polymerization of 8S clathrin to the 300S coat structure have been evaluated by turbidimetric procedures. Ca2+ and Mn2+ strongly enhance the rate of polymerization. Only spermine among the naturally occurring polybasic amine...

journal_title：Biochemistry

authors： Nandi PK,Van Jaarsveld PP,Lippoldt RE,Edelhoch H

更新日期：1981-11-10 00:00:00

abstract：:Enzymes of halophilic organisms contain unusual peptide motifs that are absent from their mesophilic counterparts. The functions of these halophile-specific peptides are largely unknown. Here we have identified an unusual peptide that is unique to several halophile archaeal cysteinyl-tRNA synthetases (CysRS), which ca...

journal_title：Biochemistry

authors： Evilia C,Hou YM

更新日期：2006-06-06 00:00:00

abstract：:Unfractionated Escherichia coli tRNA has been aminoacylated with lysine and preferentially acetylated at the epsilon-amino nitrogen of lysine by reaction with N-acetoxysuccinimide. After treatment with peptidyl-tRNA hydrolase, 90% of the aminoacylated tRNA molecules were Nepsilon-acetyl-Lys-tRNA. Post-ribosomal supern...

journal_title：Biochemistry

authors： Johnson AE,Woodward WR,Herbert E,Menninger JR

更新日期：1976-02-10 00:00:00

abstract：:A gentle and rapid isolation procedure is described yielding fractions containing better than 95% pure OmpF porin of Escherichia coli BE with different amounts of bound lipopolysaccharide (LPS). The procedure employs continuous free-flow electrophoresis (FFE) in the presence of detergent above its critical micelle con...

journal_title：Biochemistry

authors： Holzenburg A,Engel A,Kessler R,Manz HJ,Lustig A,Aebi U

更新日期：1989-05-16 00:00:00

abstract：:Ultraviolet irradiation of DNA results in various pyrimidine modifications. We studied the excision of an ultraviolet thymine photoproduct by Escherichia coli endonuclease III and by a preparation of human WI-38 cells. These enzymes cleave UV-irradiated DNA at apyrimidinic sites formed by glycosylic removal of the pho...

journal_title：Biochemistry

authors： Ganguly T,Weems KM,Duker NJ

更新日期：1990-08-07 00:00:00

abstract：:NMR spectroscopy is particularly adept at site-specifically monitoring dynamic processes in proteins, such as protein folding, domain movements, ligand binding, and side-chain rotations. By coupling the favorable spectroscopic properties of highly dynamic side-chain methyl groups with transverse-relaxation-optimized s...

journal_title：Biochemistry

authors： Boswell ZK,Latham MP

更新日期：2019-01-22 00:00:00

abstract：:The structural and functional roles of two conserved active site residues, Arg-97 and Arg-132, in chicken muscle adenylate kinase (AK) were evaluated by site-directed mutagenesis in conjunction with one- and two-dimensional proton nuclear magnetic resonance (NMR), kinetics, and guanidine hydrochloride-induced denatura...

journal_title：Biochemistry

authors： Dahnke T,Shi Z,Yan H,Jiang RT,Tsai MD

更新日期：1992-07-14 00:00:00

abstract：:Caldesmon from chicken gizzard muscle has been examined for its ability to interact with caltropin using affinity chromatography and the fluorescent probe acrylodan. The action of caltropin on the inhibitory effect of caldesmon on actomyosin ATPase was also studied. Like calmodulin, caltropin could release the inhibit...

journal_title：Biochemistry

authors： Mani RS,McCubbin WD,Kay CM

更新日期：1992-12-01 00:00:00

abstract：:Intermolecular triplex DNA is stabilized by metal cations and polyamines which reduce repulsion between the negatively charged phosphates of the three nucleic acid strands. We use a quantitative chemical-probing assay involving protection of duplex guanines in a homopyrimidine.homopurine (Py.Pu) sequence from dimethyl...

journal_title：Biochemistry

authors： Potaman VN,Sinden RR

更新日期：1995-11-14 00:00:00

abstract：:Beta(2)-microglobulin (beta(2)m) forms amyloid fibrils that deposit in the musculo-skeletal system in patients undergoing long-term hemodialysis. How beta(2)m self-assembles in vivo is not understood, since the monomeric wild-type protein is incapable of forming fibrils in isolation in vitro at neutral pH, while elong...

journal_title：Biochemistry

authors： Myers SL,Jones S,Jahn TR,Morten IJ,Tennent GA,Hewitt EW,Radford SE

更新日期：2006-02-21 00:00:00

abstract：:Pseudomonas aeruginosa PA4872 was identified by sequence analysis as a structurally and functionally novel member of the PEP mutase/isocitrate lyase superfamily and therefore targeted for investigation. Substrate screens ruled out overlap with known catalytic functions of superfamily members. The crystal structure of ...

journal_title：Biochemistry

authors： Narayanan BC,Niu W,Han Y,Zou J,Mariano PS,Dunaway-Mariano D,Herzberg O

更新日期：2008-01-08 00:00:00

abstract：:Conventional isoforms of protein kinase C (PKC) are activated when their two membrane-targeting modules, the C1 and C2 domains, bind the second messengers diacylglycerol (DG) and Ca2+, respectively. This study investigates the mechanism of Ca2+-induced binding of PKC betaII to anionic membranes mediated by the C2 doma...

journal_title：Biochemistry

authors： Nalefski EA,Newton AC

更新日期：2001-11-06 00:00:00

abstract：:The blood-clotting protein fibrinogen is composed of three subunits, designated A alpha, B beta, and gamma, which are encoded by a family of related genes. As part of the acute-phase response, expression of the fibrinogen genes is coordinately regulated in the liver by glucocorticoids. To understand the factors underl...

journal_title：Biochemistry

authors： Roberts LR,Nichols LA,Holland LJ

更新日期：1993-11-02 00:00:00

abstract：:Long-range intraprotein interactions give rise to many important protein behaviors. Understanding how energy is transduced through protein structures to either transmit a signal or elicit conformational changes is therefore a current challenge in structural biology. In an effort to understand such linkages, multiple V...

journal_title：Biochemistry

authors： Clarkson MW,Gilmore SA,Edgell MH,Lee AL

更新日期：2006-06-27 00:00:00

abstract：:The UV endonucleases [endodeoxyribonuclease (pyrimidine dimer), EC 3.1.25.1] from Micrococcus luteus and bacteriophage T4 possess two catalytic activities specific for the site of cyclobutane pyrimidine dimers in UV-irradiated DNA: a DNA glycosylase that cleaves the 5'-glycosyl bond of the dimerized pyrimidines and an...

journal_title：Biochemistry

authors： Liuzzi M,Weinfeld M,Paterson MC

更新日期：1987-06-16 00:00:00

abstract：:The 1H resonances of the high-potential [4Fe-4S]2+ ferredoxin from Chromatium vinosum have been assigned through conventional sequential methodology applied to 2D NMR spectra. Almost 80% of the residues were identified using standard 2D COSY, HOHAHA, and NOESY pulse sequences. These residues correspond to four segment...

journal_title：Biochemistry

authors： Gaillard J,Albrand JP,Moulis JM,Wemmer DE

更新日期：1992-06-23 00:00:00

abstract：:Evidence for the NADH-modulated formation of a complex between alpha-glycerol-3-phosphate dehydrogenase and l-lactate dehydrogenase was reported [Yong, H., Thomas, G. A., and Peticolas, W. L. (1993) Biochemistry 32, 11124-11131]. This NADH-modulated association suggested a mechanism of potentially great importance to ...

journal_title：Biochemistry

authors： Lehoux EA,Baker SM,Kovina MV,Hays FA,Spivey HO

更新日期：2003-05-27 00:00:00

abstract：:The serotonin transporter (SERT) is a member of a highly homologous family of proteins responsible for the reuptake of biogenic amines from the synaptic cleft. We took advantage of native restriction sites in SERT to construct a chimeric transporter containing a small (34 amino acid) region of the norepinephrine trans...

journal_title：Biochemistry

authors： Stephan MM,Chen MA,Penado KM,Rudnick G

更新日期：1997-02-11 00:00:00

abstract：:In the presence of Ca2+ and calmodulin (CM), purified smooth muscle myosin light chain kinase (MLCKase) was found to undergo autophosphorylation at a rate that was about 200-fold slower than its catalytic activity. Up to 1.7 mol of phosphate were incorporated per mole of kinase. Lower levels of incorporation could be ...

journal_title：Biochemistry

authors： Sobieszek A

更新日期：1995-09-19 00:00:00

abstract：:Cations often deform the structure of regulatory proteins to affect a functional response, but for other protein functions a more passive effect is desired. For instance, it is shown here that in the conductance of Na+ by the gramicidin channel there appears to be no significant structural deformation of either the si...

journal_title：Biochemistry

authors： Tian F,Lee KC,Hu W,Cross TA

更新日期：1996-09-17 00:00:00

abstract：:Human lysosomal enzymes acid-beta-glucosidase (GCase) and acid-alpha-galactosidase (alpha-Gal A) hydrolyze the sphingolipids glucosyl- and globotriaosylceramide, respectively, and mutations in these enzymes lead to the lipid metabolism disorders Gaucher and Fabry disease, respectively. We have investigated the structu...

journal_title：Biochemistry

authors： Lieberman RL,D'aquino JA,Ringe D,Petsko GA

更新日期：2009-06-09 00:00:00

abstract：:The reduced hydroxylase component (MMOH) of soluble methane monooxygenase (MMO) from Methylosinus trichosporium OB3b reacts with O2 and CH4 to produce CH3OH and H2O in a single-turnover reaction. Transient kinetic analysis of this reaction has revealed at least five and probably six intermediates during the turnover [...

journal_title：Biochemistry

authors： Nesheim JC,Lipscomb JD

更新日期：1996-08-06 00:00:00

abstract：:Neuroglobin (Ngb) is a recently discovered vertebrate heme protein that is expressed in the brain and can reversibly bind oxygen. Mammalian Ngb is involved in neuroprotection under oxidative stress conditions, such as ischemia and reperfusion. We previously demonstrated that human ferric Ngb binds to the alpha subunit...

journal_title：Biochemistry

authors： Watanabe S,Wakasugi K

更新日期：2008-05-13 00:00:00

abstract：:The enthalpies of transfer of hen egg white lysozyme from water to aqueous solutions of 1-propanol were determined by isothermal flow calorimetry at 10, 17, 25, and 40 degrees C in 0.04 M, pH 2 glycine buffer. Alcohol concentrations up to 3.4 M were employed. Four regions in the dependence of the enthalpy of transfer ...

journal_title：Biochemistry

authors： Sturtevant JM,Velicelebi G

更新日期：1981-05-26 00:00:00

abstract：:Binding mechanisms of ADPR-transferase to restricted double-stranded DNA fragments of SV40 and pBR322 DNA were determined by nuclease protection techniques. Top and bottom strands of double-stranded DNA were identified by specific labeling with 32P. Protection against specific exonucleases identified binding of ADPR-t...

journal_title：Biochemistry

authors： Sastry SS,Buki KG,Kun E

更新日期：1989-06-27 00:00:00

abstract：:Mutation of Pro94 to phenylalanine or alanine significantly alters the redox properties of the type I copper center of amicyanin. Each mutation increases the redox midpoint potential (E(m)) value by at least 140 mV and shifts the pK(a) for the pH dependence of the E(m) value to a more acidic value. Atomic resolution (...

journal_title：Biochemistry

authors： Carrell CJ,Sun D,Jiang S,Davidson VL,Mathews FS

更新日期：2004-07-27 00:00:00

abstract：:Marijuana is the most widely abused illegal drug, and its spectrum of effects suggests that several receptors are responsible for the activity. Two cannabinoid receptor subtypes, CB1 and CB2, have been identified, but the complex pharmacological properties of exogenous cannabinoids and endocannabinoids are not fully e...

journal_title：Biochemistry

authors： Kotsikorou E,Madrigal KE,Hurst DP,Sharir H,Lynch DL,Heynen-Genel S,Milan LB,Chung TD,Seltzman HH,Bai Y,Caron MG,Barak L,Abood ME,Reggio PH

更新日期：2011-06-28 00:00:00