Ultraviolet-induced thymine hydrates in DNA are excised by bacterial and human DNA glycosylase activities.

abstract：:The peptide alpha 1(III)-CB9 was prepared and purified from human liver, and its amino acid sequence was determined. Automated Edman degradation of the intact peptide and peptides derived from selective cleavage with hydroxylamine and digestions with trypsin, thermolysin, and Staph V8 protease enabled determination of...

journal_title：Biochemistry

authors： Seyer JM,Kang AH

更新日期：1981-04-28 00:00:00

abstract：:The dimeric nature of the HIV protease has been exploited to devise a novel mode of inhibiting the enzyme. The use of defective monomers or nonidentical subunits to exchange with wild-type homodimers produces catalytically defective heterodimers. Incubation of the HIV1 or HIV2 protease with a 4-fold molar excess of an...

journal_title：Biochemistry

authors： Babé LM,Pichuantes S,Craik CS

更新日期：1991-01-08 00:00:00

abstract：:The concept of using a dynamic base-pairing nucleobase as a mode for degenerate recognition presents a unique challenge to analysis of DNA structure. Proton and phosphorus NMR studies are reported for two nine-residue DNA oligodeoxyribonucleotides, d(CATGGGTAC).d(GTACNCATG) (1) and d(CATGTGTAC).(GTACNCATG) (2), which ...

journal_title：Biochemistry

authors： Klewer DA,Zhang P,Bergstrom DE,Davisson VJ,LiWang AC

更新日期：2001-02-13 00:00:00

abstract：:Type IV collagen promotes integrin-mediated cell adhesion, spreading, and motility. Several regions within the triple-helical domain of type IV collagen have been identified as tumor cellular recognition sites. Among these regions, the alpha1(IV)531-543 sequence, designated L-Hep-III, promotes integrin-mediated tumor ...

journal_title：Biochemistry

authors： Li C,McCarthy JB,Furcht LT,Fields GB

更新日期：1997-12-09 00:00:00

abstract：:In reticulocytes, a single ribosomal protein, S13, has been shown to be phosphorylated by the cAMP-regulated protein kinases. The 40S ribosomal subunits were phosphorylated in vitro with [gamma-32P]ATP to facilitate the identification of S13 during the two-step purification procedure. Total ribosomal protein from the ...

journal_title：Biochemistry

authors： Du Vernay VH Jr,Traugh JA

更新日期：1978-05-30 00:00:00

abstract：:The amidotransferase family of enzymes utilizes the ammonia derived from the hydrolysis of glutamine for a subsequent chemical reaction catalyzed by the same enzyme. The ammonia intermediate does not dissociate into solution during the chemical transformations. A well-characterized example of the structure and mechani...

journal_title：Biochemistry

authors： Raushel FM,Thoden JB,Holden HM

更新日期：1999-06-22 00:00:00

abstract：:Macromolecular protease inhibitors and camelid single-domain antibodies achieve their enzymic inhibition functions often through protruded structures that directly interact with catalytic centers of targeted proteases. Inspired by this phenomenon, we constructed synthetic human antibody libraries encoding long CDR-H3s...

journal_title：Biochemistry

authors： Nam DH,Lee KB,Kruchowy E,Pham H,Ge X

更新日期：2020-10-13 00:00:00

abstract：:Enzymic catalysis proceeds via intermediates formed in the course of substrate conversion. Here, we directly detect key intermediates in thiamin diphosphate (ThDP)-dependent enzymes during catalysis using (1)H NMR spectroscopy. The quantitative analysis of the relative intermediate concentrations allows the determinat...

journal_title：Biochemistry

authors： Tittmann K,Golbik R,Uhlemann K,Khailova L,Schneider G,Patel M,Jordan F,Chipman DM,Duggleby RG,Hübner G

更新日期：2003-07-08 00:00:00

abstract：:The acetylenic analogue of adenosine 9-(5',6'-dideoxy-beta-D-ribo-hex-5'-ynofuranosyl)adenine has been synthesized, and its behavior as an inhibitor of bovine S-adenosylhomocysteine hydrolase has been examined. Incubation of the enzyme with excess inhibitor caused a time-dependent, irreversible inactivation of the enz...

journal_title：Biochemistry

authors： Parry RJ,Muscate A,Askonas LJ

更新日期：1991-10-15 00:00:00

abstract：:Proteins have many distinct tertiary folds (Richardson, J. S. (1981) Adv. Prot. Chem. 34, 167-339). The term tertiary fold refers to the spatial organization of secondary structure elements (alpha-helices and beta-strands). It is not known when, in the process of protein folding, a native tertiary fold emerges. Here, ...

journal_title：Biochemistry

authors： Peng ZY,Kim PS

更新日期：1994-03-01 00:00:00

abstract：:Raman spectra of phycocyanobilin, phycocyanin, and allophycocyanin were obtained at resonance with their visible and near-UV transitions. These spectra were empirically assigned with the help of 14N- and 15N-isotopic substitutions and comparisons with resonance Raman spectra of phycoerythrin. These results confirm the...

journal_title：Biochemistry

authors： Szalontai B,Gombos Z,Csizmadia V,Bagyinka C,Lutz M

更新日期：1994-10-04 00:00:00

abstract：:Typical of many transcriptional regulatory proteins, the lambdoid bacteriophage repressors bind cooperatively to multiple sites on DNA. This cooperative binding is essential for establishment and maintenance of phage lysogeny. In the phage, two repressor homodimers, one bound at each of the adjacent operator sites, in...

journal_title：Biochemistry

authors： Ciubotaru M,Koudelka GB

更新日期：2003-04-15 00:00:00

abstract：:Sequence-specific DNA binding proteins generally consist of more than two DNA-contacting regions to ensure the selectivity of recognition. The multiple DNA binding modules are connected either through the covalent linker or through the noncovalent dimerization domain. We have compared the DNA binding of peptide dimers...

journal_title：Biochemistry

authors： Aizawa Y,Sugiura Y,Morii T

更新日期：1999-02-02 00:00:00

abstract：:Spectrin dimers interact weakly with F-actin under physiological solvent conditions (with an association constant of about 5 X 10(3) M-1 at 20 degrees C). In the presence of the membrane skeletal constituent, protein 4.1, strong binding is observed; an analysis of the profiles for formation of a ternary complex leads ...

journal_title：Biochemistry

authors： Ohanian V,Wolfe LC,John KM,Pinder JC,Lux SE,Gratzer WB

更新日期：1984-09-11 00:00:00

abstract：:The far-ultraviolet circular dichroism spectra of bovine and human prothrombin, prothrombin fragment 1, prethrombin 1, prothrombin fragment 2, and prethrombin 2 (prethrombin 2des(1--13)) were determined and the method of Chen et al. [Chen, Y. H., Yang, J. T., & Martinez, H. M. (1972) Biochemistry 11, 4120--4131; Chen,...

journal_title：Biochemistry

authors： Bloom JW,Mann KG

更新日期：1979-05-15 00:00:00

abstract：:The linear noncompetitive inhibition of the pepsin-catalyzed hydrolysis of Ac-Phe-Phe-Gly at pH 2.1 by L-Ac-Phe, L-Ac-Phe-NH2, and L-Ac-Phe-OEt has been claimed to substantiate the ordered release of products specified by the amino-enzyme mechanism for pepsin action. According to this interpretation, the binding of in...

journal_title：Biochemistry

authors： Silver MS,Stoddard M

更新日期：1975-02-11 00:00:00

abstract：:Smooth muscle cells maintain filaments of actin and myosin in the presence of ATP, although dephosphorylated myosin filaments and actin-myosin interactions are unstable under those conditions in vitro. Several proteins that stabilize myosin filaments and that stabilize actin-myosin interactions have been identified. F...

journal_title：Biochemistry

authors： Kingsbury NL,Renegar RH,Chalovich JM

更新日期：2013-10-29 00:00:00

abstract：:The l-lyxonate dehydratase (LyxD) in vitro enzymatic activity and in vivo metabolic function were assigned to members of an isofunctional family within the mandelate racemase (MR) subgroup of the enolase superfamily. This study combined in vitro and in vivo data to confirm that the dehydration of l-lyxonate is the bio...

journal_title：Biochemistry

authors： Ghasempur S,Eswaramoorthy S,Hillerich BS,Seidel RD,Swaminathan S,Almo SC,Gerlt JA

更新日期：2014-05-27 00:00:00

abstract：:The primary structure of a multifunctional protein, the large alpha-subunit of the Escherichia coli fatty acid oxidation complex, was determined by sequencing the fadB region of the fadBA operon. The amino-terminal sequence of this protein had been established by Edman degradation. The transcription start site of the ...

journal_title：Biochemistry

authors： Yang XY,Schulz H,Elzinga M,Yang SY

更新日期：1991-07-09 00:00:00

abstract：:A substrate analogue, 6-(difluoromethyl)tryptophan, was developed and characterized for mechanistic investigation of tryptophanase. The utility of this derivative was based on its ability to partition between fluoride elimination and carbon-carbon bond scission during tryptophan metabolism. The non-enzymatic hydrolysi...

journal_title：Biochemistry

authors： Woolridge EM,Rokita SE

更新日期：1991-02-19 00:00:00

abstract：:Glutaminase (GLS1/2) catalyzes the conversion of L-glutamine to L-glutamate and ammonia. The level of a splice variant of GLS1 (GAC) is elevated in certain cancers, and GAC is specifically inhibited by bis-2-(5-phenylacetimido-1,2,4,thiadiazol-2-yl)ethyl sulfide (BPTES). We report here the first full-length crystal st...

journal_title：Biochemistry

authors： DeLaBarre B,Gross S,Fang C,Gao Y,Jha A,Jiang F,Song J J,Wei W,Hurov JB

更新日期：2011-12-20 00:00:00

abstract：:A study of the voltage induction of transient pores in phospholipid bilayer vesicles is reported. Unilamellar vesicles (dipalmitoylphosphatidylcholine), with a size distribution of 100 +/- 30 nm, were prepared by the method of Enoch & Strittmatter [Enoch, H., & Strittmatter, P. (1979) Proc. Natl. Acad. Sci. U.S.A. 76,...

journal_title：Biochemistry

authors： Teissie J,Tsong TY

更新日期：1981-03-17 00:00:00

abstract：:The physiological roles of immunophilins are unclear, but many possess peptidyl-prolyl isomerase (PPIase) activity, and they have been found in all organisms examined to date, implying that they are involved in fundamental, protein-folding processes. The chloroplast thylakoid lumen of the higher plant Arabidopsis thal...

journal_title：Biochemistry

authors： Edvardsson A,Shapiguzov A,Petersson UA,Schröder WP,Vener AV

更新日期：2007-08-21 00:00:00

abstract：:A novel truncated form (residues 1-214, with a randomized C-terminal tail) of the ligand-binding extracellular domain (ECD) of the human alpha1 glycine receptor (GlyR), with amino acids from the corresponding sequence of an acetylcholine binding protein (AChBP) substituted for two relatively hydrophobic membrane-proxi...

journal_title：Biochemistry

authors： Liu Z,Ramanoudjame G,Liu D,Fox RO,Jayaraman V,Kurnikova M,Cascio M

更新日期：2008-09-16 00:00:00

abstract：:The majority of pre-steady-state kinetic investigations with HIV-1 reverse transcriptase (HIV-1 RT) have reported substoichiometric bursts (30-50%) of product formation in the initial reaction cycle. By using quantitative amino acid analysis, we have revised the extinction coefficient of the HIV-1 RT heterodimer and s...

journal_title：Biochemistry

authors： Furge LL,Guengerich FP

更新日期：1999-04-13 00:00:00

abstract：:The monoamine transporter of the chromaffin granule membranes can be specifically labeled by the photoaffinity reagent 7-azido-8-[125I]iodoketanserin. The characteristics of the labeled protein have been investigated. Two-dimensional gel electrophoresis of the labeled membranes indicated a MW of about 70,000 and an is...

journal_title：Biochemistry

authors： Isambert MF,Gasnier B,Botton D,Henry JP

更新日期：1992-02-25 00:00:00

abstract：:SopE and SopE2 are delivered by the Salmonella type III secretion system into eukaryotic cells to promote cell invasion. SopE and SopE2 are potent guanine nucleotide exchange factors (GEFs) for Rho GTPases Cdc42 and Rac1 and constitute a novel class of Rho GEFs. Although the sequence of SopE-like GEFs is not at all ho...

journal_title：Biochemistry

authors： Williams C,Galyov EE,Bagby S

更新日期：2004-09-28 00:00:00

abstract：:The inhibitory effects of urea on the normally rapid fibrillation of human calcitonin (hCT) were investigated by 1H NMR. From subtle differences in the chemical shift of hCT in the presence and absence of urea, the occurrence of weak interactions between urea and hCT was confirmed. The chemical shifts on the NH and C ...

journal_title：Biochemistry

authors： Kanaori K,Nosaka AY

更新日期：1996-10-01 00:00:00

abstract：:Mdm2 negatively regulates p53 by inhibiting its transcriptional activity and promoting its degradation by functioning as an E3 ubiquitin ligase. The primary p53 binding site on mdm2 is located in its N-terminal domain. Through binding to p53 at its N-terminal transactivation domain, mdm2 directly blocks the transcript...

journal_title：Biochemistry

authors： Ma J,Martin JD,Zhang H,Auger KR,Ho TF,Kirkpatrick RB,Grooms MH,Johanson KO,Tummino PJ,Copeland RA,Lai Z

更新日期：2006-08-01 00:00:00

abstract：:The gene encoding enzyme IIA(glc) (EIIA) of the phosphoenolpyruvate:sugar phosphotransferase system of Mycoplasma capricolum was cloned into a regulated expression vector. The purified protein product of the overexpressed gene was characterized as an active phosphoacceptor from HPr with a higher pI than previously des...

journal_title：Biochemistry

authors： Zhu PP,Nosworthy N,Ginsburg A,Miyata M,Seok YJ,Peterkofsky A

更新日期：1997-06-10 00:00:00