Abstract:
:Resonance Raman spectra of the photosynthetic reaction center isolated from the green bacterium Chloroflexus aurantiacus have been obtained with excitation in the near-infrared absorption bands of the special pair (P) and the accessory bacteriochlorophyll (B) using shifted-excitation Raman difference spectroscopy (SERDS). These spectra are compared with the previously reported Raman spectra of P and B in reaction centers from the purple bacterium Rhodobacter sphaeroides. The spectra of P and B from the two species are nearly identical. Common and distinctive attributes of these spectra include enhanced low-frequency (30-200 cm-1) modes in P and the absence of strong Raman activity in modes higher than 1200 cm-1 in both P and B. Also, the absolute scattering cross sections with excitation in the P band are unusually weak in both reaction centers, indicating that their excited states are rapidly vibronically dephased. The striking similarities between the P and B spectra in reaction centers from two very different bacterial species suggest that the common nuclear and electronic dynamics identified here are characteristic of photosynthetic reaction centers.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Cherepy NJ,Holzwarth AR,Mathies RAdoi
10.1021/bi00015a044subject
Has Abstractpub_date
1995-04-18 00:00:00pages
5288-93issue
15eissn
0006-2960issn
1520-4995journal_volume
34pub_type
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