Abstract：
:Intracellular retinoid-binding proteins are small, tightly folded, compact proteins, which appear to be involved in the delivery of retinoids to microsomal metabolic enzymes, among other potential roles. Recently, it has been demonstrated that two of these binding proteins, cellular retinol-binding protein (CRBP) and cellular retinol-binding protein type II [CRBP(II)], interact with the same microsomal enzyme but in different manners, depending on the absence or presence of ligand [Herr, F.M., & Ong, D.E. (1992) Biochemistry 31, 6748-6755]. The structural components of the binding proteins responsible for these differential interactions are presently unknown. In addition, it is not clear how the ligand is able to gain entry into the solvent-inaccessible interior binding cavity. Limited proteolysis of the apo and holo forms of CRBP and CRBP(II) was used to probe the conformational differences between the different states of these two proteins in solution. It was found that the apo forms of both proteins were significantly more susceptible to proteolysis, and probably adopted a more open conformation, than the holo forms. The initial cleavage site of endoproteinase Arg-C in the apo forms occurred at a conserved arginine residue near a possible site of ligand entry. Similar results were obtained by limited proteolysis of cellular retinoic acid-binding protein and heart fatty acid-binding protein, indicating that a common ligand-induced conformational change may occur for other members of this family of intracellular binding proteins.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Jamison RS,Newcomer ME,Ong DEdoi
10.1021/bi00176a017subject
Has Abstractpub_date
1994-03-15 00:00:00pages
2873-9issue
10eissn
0006-2960issn
1520-4995journal_volume
33pub_type
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