Abstract:
:Type B streptogramins, such as virginiamycin S (VS), are cyclic hexadepsipeptides, inhibiting protein synthesis in prokaryotes. L-Thr connects a 3-hydroxypicolinyl residue (3-OH-Pic) to the peptide lactone ring. The fluorescence intensity of 3-OH-Pic is strongly increased by chelation to alkaline earth cations or binding to ribosomes. Similar behavior of the ribosome-VS complex and the VS-Mg chelate provides strong evidence for the presence of a VS-Mg chelate within the ribosomal binding site. Different models involving the ribosome binding of either members of the VS-Mg2+ chelate or both have been tested by fluorescence lifetime measurements, equilibrium titrations, and stopped-flow spectrofluorometry. Our data strongly suggest that (a) the interaction between VS and the ribosome is partly provided by a salt bridge between suitable acceptor atoms of the ribosome and the 3-OH-Pic residue, (b) Mg2+ can be exchanged by Mn2+ without dissociation of the ribosome-VS complex, (c) Mg2+ coordinates to the negative form of the 3-OH-Pic residue, probably via an interaction with the phenolate oxygen and the amide carboxyl group, and (d) the picolinyl residue is essential for the biological activity, as indicated by the lack of activity when the latter is replaced by a serine derivative.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Di Giambattista M,Engelborghs Y,Nyssen E,Clays K,Cocito Cdoi
10.1021/bi00243a033subject
Has Abstractpub_date
1991-07-23 00:00:00pages
7277-82issue
29eissn
0006-2960issn
1520-4995journal_volume
30pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Physicochemical properties of recombinant human erythropoietin were examined. This protein, produced in Chinese hamster ovary cells, showed a conformation apparently identical with the natural product isolated from human urine when examined by circular dichroism, UV absorbance, and fluorescence spectroscopy. Sedimenta...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00383a034
更新日期:1987-05-05 00:00:00
abstract::Nitroxyl (HNO), a potential heart failure therapeutic, is known to target cysteine residues to form sulfinamides and/or disulfides. Because HNO-derived modifications may depend on their local environment, we have investigated the reactivity of HNO with cysteine derivatives and C-terminal cysteine-containing peptides a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500360x
更新日期:2014-06-10 00:00:00
abstract::Regulation of cellular processes by dietary nutrients is known to affect the likelihood of cancer development. One class of cancer-preventive nutrients, isothiocyanates (ITCs), derived from the consumption of cruciferous vegetables, is known to have various effects on cellular biochemistry. One target of ITCs is macro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3005494
更新日期:2012-09-25 00:00:00
abstract::Upon cleavage of the reactive thioester bonds (Cys-949-Glx-952) of tetrameric human alpha 2-macroglobulin (alpha 2M) by methylamine, one sulfhydryl group per alpha 2M subunit is exposed. These identical sulfhydryl group sites were labeled with the thiol-specific nitroxide spin-labels (1-oxy-2,2,5,5-tetramethyl-3-pyrro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00414a053
更新日期:1988-07-12 00:00:00
abstract::When the single-stranded RNA genome of HIV-1 is copied into double-stranded DNA, the viral enzyme reverse transcriptase (RT) catalyzes the addition of approximately 20,000 nucleotides; however, the precise mechanism of nucleotide addition is unknown. In this study, we attempt to integrate the genetic data and biochemi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00016a006
更新日期:1995-04-25 00:00:00
abstract::The role of the Na,K-ATPase beta-subunit in stabilization of ion-binding sites has been investigated. Treatment of the purified renal Na,K-ATPase with 0.25 M DTT at 40 degrees C for 1 h resulted in 50% loss of Rb occlusion, which correlates with partial reduction of S-S bridges in the extracellular portion of the beta...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00077a029
更新日期:1993-07-06 00:00:00
abstract::The P-glycoprotein multidrug transporter functions as an ATP-driven efflux pump for a large number of structurally unrelated hydrophobic compounds. Substrates are believed to gain access to the transporter after partitioning into the membrane, rather than from the extracellular aqueous phase. The binding of drug subst...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990064q
更新日期:1999-05-25 00:00:00
abstract::Human transcriptional coactivator P/CAF (p300/CBP-associating factor) is a histone acetyltransferase (HAT) and is a member of the GNAT (GCN5 related N-acetyltransferases) superfamily. P/CAF was originally identified by its ability to activate transcription of a variety of genes through its interaction with p300/CBP. T...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001272h
更新日期:2000-10-03 00:00:00
abstract::PEP mutase catalyzes the conversion of phosphoenolpyruvate (PEP) to phosphonopyruvate in biosynthetic pathways leading to phosphonate secondary metabolites. A recent X-ray structure [Huang, K., Li, Z., Jia, Y., Dunaway-Mariano, D., and Herzberg, O. (1999) Structure (in press)] of the Mytilus edulis enzyme complexed wi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990771j
更新日期:1999-10-26 00:00:00
abstract::Human alpha1 and alpha2 isoforms of Na,K-ATPase have been expressed with porcine 10*Histidine-tagged beta1 subunit in Pichia pastoris. Methanol-induced expression of alpha2 is optimal at 20 degrees C, whereas at 25 degrees C, which is optimal for expression of alpha1, alpha2 is not expressed. Detergent-soluble alpha2b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701812c
更新日期:2007-12-25 00:00:00
abstract::The complete amino acid sequence of ragweed pollen allergen Ra3 has been determined. The molecule consists of 101 amino acid residues and to date is the only allergen isolated from Ambrosia elatior (short ragweed) which contains carbohydrate. This particular preparation of allergen has a single, unique amino acid sequ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00566a010
更新日期:1980-12-09 00:00:00
abstract::The formation and role of arachidonic acid (AA) and its metabolites during gonadotropin releasing hormone- (GnRH-) induced gonadotropin secretion were investigated in primary cultures of rat pituitary cells. Prelabeled cells ([3H]AA) responded to GnRH challenge with increased formation (about 2-fold) of the leukotrien...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00139a004
更新日期:1992-06-23 00:00:00
abstract::Mutations in the Kirsten ras (KRAS) gene are present in almost all pancreatic adenocarcinomas, and one common mutation is at codon 12: GGT (Gly) is transformed into GAT (Asp). In this work we have targeted the KRAS coding sequence embracing the GAT mutation with a sense PNA molecule (P14), with the aim of downregulati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0505215
更新日期:2005-08-09 00:00:00
abstract::4-Oxalocrotonate tautomerase (EC 5.3.2-; 4-OT), a hexamer consisting of 62 residues per subunit, catalyzes the isomerization of unsaturated alpha-keto acids, converting unconjugated ketones to the conjugated isomers via a dienolic intermediate. The recently solved crystal structure of an isozyme of 4-OT suggests that ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951077g
更新日期:1996-01-23 00:00:00
abstract::HK31 (S565-K595) has previously been shown to encompass the binding domain for plasma prekallikrein (PK) within domain 6 of high molecular weight kininogen (HK). The complementary binding domain for HK within PK is mapped to PK56 (F56-G86), in the apple 1 domain, and to PK266 (K266-C295), in the apple 4 domain. Isothe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960547j
更新日期:1996-10-01 00:00:00
abstract::Rhodospirillum rubrum ribulose bisphosphate carboxylase contains two high affinity binding sites for pyridoxal phosphate and two catalytic sites per dimer. However, pyridoxal phosphate binding at only one site is sufficient for inactivation of both catalytic sites. In the presence of 20 mM bicarbonate, 10 mM magnesium...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00600a024
更新日期:1978-04-04 00:00:00
abstract::The highly conserved nature and tissue specificity of the seven vertebrate beta-tubulin isotypes provide circumstantial evidence that functional differences among isotypes may exist in vivo. Compelling evidence from studies of bovine brain beta-isotypes indicated significant conformational and functional differences i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972763d
更新日期:1998-03-31 00:00:00
abstract::When rabbits are fed a diet supplemented with cholesterol, their plasma cholesterol levels increase markedly, and they develop atherosclerosis. Most of the plasma cholesterol exists as cholesteryl esters in very low density lipoproteins (VLDL) and intermediate-density lipoproteins (IDL). The triglyceride content of th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00268a025
更新日期:1982-12-07 00:00:00
abstract::Recent structural studies of the HMG-CoA synthase members of the thiolase superfamily have shown that the catalytic loop containing the nucleophilic cysteine follows the phi and psi angle pattern of a II' beta turn. However, the i + 1 residue is conserved as an alanine, which is quite unusual in this position as it mu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061505q
更新日期:2006-12-05 00:00:00
abstract::We have studied the role of amphipathic alpha-helices in the ability of apoA-I to promote cholesterol efflux from human skin fibroblasts and activate lecithin:cholesterol acyltransferase (LCAT). Three apoA-I mutants were designed, each by deletion of a pair of predicted adjacent central alpha-helices [Delta(100-143), ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981205b
更新日期:1998-09-29 00:00:00
abstract::Biotin synthase (BioB) is an iron-sulfur dimeric enzyme which catalyzes the last step in biotin synthesis. The reaction consists of the introduction of a sulfur atom into dethiobiotin. It is shown here that BioB displays a significant cysteine desulfurase activity, providing it with the ability to mobilize sulfur from...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0122011
更新日期:2002-07-23 00:00:00
abstract::The motion of the ethanolamine head group in unsonicated lipid bilayers above and below the phase transition is studied by means of deuterium and phosphorus magnetic resonance. For this purpose, dipalmitoyl-3-sn-phosphatidylethanolamine is selectively deuterated at the two ethanolamine carbon atoms. The deuterium quad...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00669a001
更新日期:1976-11-30 00:00:00
abstract::To study the engineering requirements for proton pumping in energy-converting enzymes such as cytochrome c oxidase, the thermodynamics and mechanisms of proton-coupled electron transfer in two designed heme proteins are elucidated. Both heme protein maquettes chosen, heme b-[H10A24]2 and heme b-[delta7-His]2, are four...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061607g
更新日期:2007-01-09 00:00:00
abstract::Herpesvirus saimiri codes for a tyrosine kinase interacting protein (Tip) that interacts with both the SH3 domain and the kinase domain of the T-cell-specific tyrosine kinase Lck via two separate motifs. The activation of Lck by Tip is considered as a key event in the transformation of human T-lymphocytes during herpe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015986j
更新日期:2002-04-23 00:00:00
abstract::In the primary quinone (Q(A)) binding site of Rb. sphaeroides reaction centers (RCs), isoleucine M265 is in extensive van der Waals contact with the ubiquinone headgroup. Substitution of threonine or serine for this residue (mutants M265IT and M265IS), but not valine (mutant M265IV), lowers the redox midpoint potentia...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026958j
更新日期:2003-04-15 00:00:00
abstract::A galactoside-binding lectin (hL-31) containing a collagen-like sequence was identified in human tumor cells. It was found to be the homologue of the IgE-binding protein, the macrophage cell-surface Mac-2 antigen, and the murine CBP35, RL-29, and mL-34 lectins. Here we report on the expression in Escherichia coli and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00067a038
更新日期:1993-04-27 00:00:00
abstract::Several mutant Escherichia coli aspartate aminotransferases (eAATases) have been characterized in the attempt to evolve or rationally redesign the substrate specificity of eAATase into that of E. coli tyrosine aminotransferase (eTATase). These include HEX (designed), HEX + A293D (design followed by directed evolution)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0487544
更新日期:2004-10-12 00:00:00
abstract::Identification of the kinetic intermediates in GTP hydrolysis on microtubules and characterization of their assembly properties is essential in understanding microtubule dynamics. By using an improved glass filter assay that selectively traps microtubules with a dead time of 2 s and monitoring taxol-induced rapid asse...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00490a007
更新日期:1990-09-25 00:00:00
abstract::Distance constraints from two-dimensional NMR cross-relaxation data are used to derive a three-dimensional structure for acyl carrier protein from Escherichia coli. Several approaches to structure determination are explored. The most successful proves to be an approach that combines the early stages of a distance geom...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00416a046
更新日期:1988-08-09 00:00:00
abstract::There is considerable evidence that long-range interactions stabilize residual protein structure under denaturing conditions. However, evaluation of the effect of a specific contact on structure in the denatured state has been difficult. Iso-1-cytochrome c variants with a Lys54 → His mutation form a particularly stabl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01002
更新日期:2017-12-26 00:00:00