Direct evidence for GTP and GDP-Pi intermediates in microtubule assembly.

abstract：:N,N'-dicyclohexylcarbodiimide (DCCD) has been reported to inhibit proton translocation by cytochrome bc(1) and b(6)f complexes without significantly altering the rate of electron transport, a process referred to as decoupling. To understand the possible role of DCCD in inhibiting the protonogenic reactions of cytochro...

journal_title：Biochemistry

authors： Shinkarev VP,Ugulava NB,Crofts AR,Wraight CA

更新日期：2000-12-26 00:00:00

abstract：:The borrelidin polyketide synthase (PKS) begins with a carboxylated substrate and, unlike typical decarboxylative loading PKSs, retains the carboxy group in the final product. The specificity and tolerance of incorporation of carboxyacyl substrate into type I PKSs have not been explored. Here, we show that the first e...

journal_title：Biochemistry

authors： Hagen A,Poust S,de Rond T,Yuzawa S,Katz L,Adams PD,Petzold CJ,Keasling JD

更新日期：2014-09-30 00:00:00

abstract：:Myeloperoxidase plays a fundamental role in oxidant production by neutrophils. The enzyme uses hydrogen peroxide to oxidize chloride (Cl-), bromide (Br-), iodide (I-), and the pseudohalide thiocyanate (SCN-) to their respective hypohalous acids. This study for the first time presents transient kinetic measurements of ...

journal_title：Biochemistry

authors： Furtmüller PG,Burner U,Obinger C

更新日期：1998-12-22 00:00:00

abstract：:Chemical and enzymatic probing (footprinting) of the reactivity of the promoter DNA backbone is applied to characterize two binary open complexes RPo1 (-Mg2+) and RPo2 (+Mg2+), formed by Escherichia coli RNA polymerase (E sigma 70) at the lambda PR promoter. We report that HO. detects major differences in backbone rea...

journal_title：Biochemistry

authors： Craig ML,Suh WC,Record MT Jr

更新日期：1995-12-05 00:00:00

abstract：:Twenty-five different temperature-sensitive point mutations at 20 sites in the lysozyme gene of bacteriophage T4 have been identified. All of the mutations alter amino acid side chains that have lower than average crystallographic thermal factors and reduced solvent accessibility in the folded protein. This suggests t...

journal_title：Biochemistry

authors： Alber T,Sun DP,Nye JA,Muchmore DC,Matthews BW

更新日期：1987-06-30 00:00:00

abstract：:The chicken and goose egg white lysozymes (ChEWL and GoEWL) are homologues, but differ in substrate specificity. ChEWL catalyzes the hydrolysis of the glycosidic bonds of bacterial peptidoglycans and chitin-derived substrates, while GoEWL is specific for bacterial peptidoglycans. The active-site aspartate 52 residue o...

journal_title：Biochemistry

authors： Matsumura I,Kirsch JF

更新日期：1996-02-13 00:00:00

abstract：:Prion diseases are associated with the conversion of the normal prion protein, PrP(C), to the infectious disease form PrP(Sc). Discrimination between these isoforms would significantly enhance diagnosis of these diseases, and it has recently been reported that PrP(Sc) is specifically recognized by the serine protease ...

journal_title：Biochemistry

authors： Ellis V,Daniels M,Misra R,Brown DR

更新日期：2002-06-04 00:00:00

abstract：:Urea lesions are formed in DNA because of free radical damage of the thymine base, and their occurrence in DNA blocks DNA polymerases, which has deleterious consequences. Recently, it has been shown that urea is capable of forming hydrogen bonding and stacking interactions with nucleobases, which are responsible for t...

journal_title：Biochemistry

authors： Suresh G,Padhi S,Patil I,Priyakumar UD

更新日期：2016-10-11 00:00:00

abstract：:Salts and polyamines have a variety of effects on the physical properties of DNA, including stabilization against thermal melting. We wished to gain greater insight into the mechanism of this stabilization by ascertaining its effect on the dynamics of base opening and closing reactions, as measured by NMR. Since the b...

journal_title：Biochemistry

authors： Braunlin WH,Bloomfield VA

更新日期：1988-02-23 00:00:00

abstract：:This paper reports on a solution NMR characterization of the sequence selectivity and metal ion specificity in chromomycin-DNA oligomer complexes in the presence of divalent cations. The sequence selectivity studies have focused on chromomycin complexes with the self-complementary d(A1-A2-G3-G4-C5-C6-T7-T8) duplex con...

journal_title：Biochemistry

authors： Gao XL,Patel DJ

更新日期：1990-12-11 00:00:00

abstract：:The protein composition, steady state and time-resolved fluorescence emission spectra were studied in solubilized and aggregated LHCII complexes, that were prepared according to two different isolation protocols: (1) by fractionation of cation-depleted thylakoid membranes using the non-ionic detergent Triton X-100 acc...

journal_title：Biochemistry

authors： Vasil'ev S,Irrgang KD,Schrötter T,Bergmann A,Eichler HJ,Renger G

更新日期：1997-06-17 00:00:00

abstract：:N-Ethylmaleimide (NEM), which reacts readily with exposed sulfhydryl groups, has been shown to inhibit the activity of the microtubule (MT) motors kinesin, Ncd, and dynein. Currently, the mechanism of inhibition is not known for any of these proteins. To investigate the mechanism by which NEM inhibits Ncd, the recombi...

journal_title：Biochemistry

authors： Phelps KK,Walker RA

更新日期：1999-08-17 00:00:00

abstract：:Studies are presented of the equilibrium binding of [3H]-d-tubocurarine (dTC) and [3H]acetylcholine (AcCh) to Torpedo postsynaptic membranes. The saturable binding of [3H]dTC is characterized by two affinities: Kd1 = 33 +/- 6 nM and Kd2 = 7.7 +/- 4.6 microM, with equal numbers of binding sites. Both components are com...

journal_title：Biochemistry

authors： Neubig RR,Cohen JB

更新日期：1979-11-27 00:00:00

abstract：:We have previously shown that GTP can replace ATP as an energy source to support vinblastine transport by the multidrug transporter P-glycoprotein (Pgp) in plasma membrane vesicles isolated from the multidrug resistant cell line KB-V1 [Lelong et al. (1992) FEBS Lett. 304, 256-260]. Like [gamma-32P]ATP, [gamma-32P]GTP ...

journal_title：Biochemistry

authors： Lelong IH,Cardarelli CO,Gottesman MM,Pastan I

更新日期：1994-08-02 00:00:00

abstract：:The ability to undergo large-scale domain rearrangements is essential for the substrate-binding function of periplasmic binding proteins (PBPs), which are indispensable for nutrient uptake in Gram-negative bacteria. Crystal structures indicate that PBPs typically adopt either an "open" unliganded configuration or a "c...

journal_title：Biochemistry

authors： Bermejo GA,Strub MP,Ho C,Tjandra N

更新日期：2010-03-09 00:00:00

abstract：:Actinaria cytolysins are very potent basic toxins isolated from the venom of sea anemones, which are supposed to exert their toxic activity through formation of oligomeric pores in the host plasma membrane. To gain insight into their mechanism of action, the interaction of Stichodactyla helianthus sticholysin I (St-I)...

journal_title：Biochemistry

authors： Tejuca M,Serra MD,Ferreras M,Lanio ME,Menestrina G

更新日期：1996-11-26 00:00:00

abstract：:Several snake venoms contain a phospholipase A2 in which position 49 in the active site is occupied by a lysine or a serine instead of the aspartate residue normally found. Although these proteins do not bind Ca2+ and are devoid of catalytic activity, they are still highly specific myotoxins and have recently been sho...

journal_title：Biochemistry

authors： Pedersen JZ,Lomonte B,Massoud R,Gubensek F,Gutiérrez JM,Rufini S

更新日期：1995-04-11 00:00:00

abstract：:End binding protein 1 (EB1) and cytoplasmic linker protein of 170 kDa (CLIP-170) are two well-studied microtubule plus-end-tracking proteins (+TIPs) that target growing microtubule plus ends in the form of comet tails and regulate microtubule dynamics. However, the mechanism by which they regulate microtubule dynamics...

journal_title：Biochemistry

authors： Lopus M,Manatschal C,Buey RM,Bjelić S,Miller HP,Steinmetz MO,Wilson L

更新日期：2012-04-10 00:00:00

abstract：:The mitochondrial tyrosyl tRNA synthetase from Neurospora crassa (CYT-18 protein) is a bifunctional group I intron splicing cofactor. CYT-18 is capable of splicing multiple group I introns from a wide variety of sources by stabilizing the catalytically active intron structures. CYT-18 and mt TyrRSs from related fungal...

journal_title：Biochemistry

authors： Geng C,Paukstelis PJ

更新日期：2014-03-04 00:00:00

abstract：:Ankyrin repeat (AR) proteins possess a distinctive modular and repetitive architecture, and their global folds are maintained by short-range interactions in terms of the primary sequence. In this work, we extended our previous study on the highly conserved TPLH tetrapeptide and investigated the impact of a solvent-exp...

journal_title：Biochemistry

authors： Yuan C,Guo Y,Zhu L,Guo W,Mahajan A,Weghorst CM,Li J

更新日期：2013-07-16 00:00:00

abstract：:We have systematically validated the activity and inhibition of a HIV-1 protease (PR) variant bearing 17 mutations (PR(S17)), selected to represent high resistance by machine learning on genotype-phenotype data. Three of five mutations in PR(S17) correlating with major drug resistance, M46L, G48V, and V82S, and five o...

journal_title：Biochemistry

authors： Park JH,Sayer JM,Aniana A,Yu X,Weber IT,Harrison RW,Louis JM

更新日期：2016-04-26 00:00:00

abstract：:Mycoplasmas are the smallest known microorganisms, with drastically reduced genome sizes. One of the essential biochemical pathways lost in mycoplasmas is methylation-mediated DNA repair (MMR), which is responsible for correction of base substitutions, insertions, and deletions in both bacteria and higher organisms. W...

journal_title：Biochemistry

authors： Kamashev D,Oberto J,Serebryakova M,Gorbachev A,Zhukova Y,Levitskii S,Mazur AK,Govorun V

更新日期：2011-10-11 00:00:00

abstract：:The highly cooperative binding of fd gene 5 to single-stranded DNA was studied kinetically by rapid photo-cross-linking and stopped-flow UV absorption measurements. The observed change in absorbance was shown to be due to the binding by direct evidence of rapid photo-cross-linking of the bound proteins to fd DNA. The ...

journal_title：Biochemistry

authors： Shimamoto N,Utiyama H

更新日期：1983-12-06 00:00:00

abstract：:Measurements of the magnetic field dependence of the longitudinal magnetic relaxation rates (NMRD profiles) of solvent protons and deuterons led to the discovery of two classes of solvent binding sites in Ca2+-Mn2+-concanavalin A (CMPL) [Koenig, S. H., Brown, R. D., III, & Brewer, C. F. (1985) Biochemistry (second of ...

journal_title：Biochemistry

authors： Bhattacharyya L,Brewer CF,Brown RD 3rd,Koenig SH

更新日期：1985-09-10 00:00:00

abstract：:The interaction of the so-called fusion peptide of the human immunodeficiency virus gp41 envelope glycoprotein with the target cell membrane is believed to trigger the fusion process which allows the entry of the virus into the cell. Many studies on the interaction of the fusion peptide with biological membranes have ...

journal_title：Biochemistry

authors： Buzón V,Padrós E,Cladera J

更新日期：2005-10-11 00:00:00

abstract：:Phenylcarbene and adamantylidene have been generated photochemically from the corresponding diazirines within lipid bilayers. Reasonable yields of labeled fatty acid side chains have been observed. The products have been characterized by gas chromatography-mass spectrometry and derive both from the insertion of the c...

journal_title：Biochemistry

authors： Bayley H,Knowles JR

更新日期：1978-06-13 00:00:00

abstract：:The two-finger repeat in the human male-associated protein ZFY provides a model for comparative 2D-NMR studies of classical and variant Zn fingers. This repeat is defined in part by an alternation in spacing between consensus (HX3H) and variant (HX4H) histidine spacings. To investigate the effects of a "switch" betwee...

journal_title：Biochemistry

authors： Kochoyan M,Keutmann HT,Weiss MA

更新日期：1991-10-01 00:00:00

abstract：:1H NMR spectral editing techniques can select the distinct signals of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate and provide a unique way to monitor the biochemical processes in vivo. These metabolite levels reflect the near-equilibrium dehydrogenase activity and therefore the cellular redox state. The ...

journal_title：Biochemistry

authors： Chung Y,Jue T

更新日期：1992-11-17 00:00:00

abstract：:A reinvestigation of the metal ion activation and specificity of CTP synthetase was begun in order to separate effects due to binding of free metal ions and binding of nucleotide-complexed ions. Apo-CTP synthetase was prepared by dialysis against 5 mM EDTA. Analysis of apo-enzyme by atomic absorption spectroscopy reve...

journal_title：Biochemistry

authors： Robertson JG,Villafranca JJ

更新日期：1993-04-13 00:00:00

abstract：:Thermodynamic equilibrium transition models in DSC are only applicable to reversible processes, but reversibility of the thermal transitions of proteins is comparatively rare because of intermolecular aggregation of denatured proteins and the degradation that occurs at high temperatures. The cupredoxin azurin from Pse...

journal_title：Biochemistry

authors： Sandberg A,Leckner J,Shi Y,Schwarz FP,Karlsson BG

更新日期：2002-01-22 00:00:00