An in vitro peptide complementation assay for CYT-18-dependent group I intron splicing reveals a new role for the N-terminus.

abstract：:The complete amino acid sequence of the major component myoglobin from the dwarf sperm whale, Kogia simus, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequenator. Three easily separable peptides were obtained by cleaving the protein at its two...

journal_title：Biochemistry

authors： Dwulet FE,Jones BN,Lehman LD,Gurd FR

更新日期：1977-03-08 00:00:00

abstract：:The specificity of subtilisin BPN' toward substrates with large hydrophobic P4 residues has been improved by single amino acid replacements at positions 104 and 107. Mutations were designed to (i) increase the size of the P4 binding pocket by replacing Ile107, which is at the bottom of the S4 pocket, by Val, Ala, and ...

journal_title：Biochemistry

authors： Rheinnecker M,Baker G,Eder J,Fersht AR

更新日期：1993-02-09 00:00:00

abstract：:The solution nuclear magnetic resonance (NMR) structures and backbone (15)N dynamics of the specialized acyl carrier protein (ACP), RpAcpXL, from Rhodopseudomonas palustris, in both the apo form and holo form modified by covalent attachment of 4'-phosphopantetheine at S37, are virtually identical, monomeric, and corre...

journal_title：Biochemistry

authors： Ramelot TA,Rossi P,Forouhar F,Lee HW,Yang Y,Ni S,Unser S,Lew S,Seetharaman J,Xiao R,Acton TB,Everett JK,Prestegard JH,Hunt JF,Montelione GT,Kennedy MA

更新日期：2012-09-18 00:00:00

abstract：:The equilibrium between the rat brain tubulin alpha beta dimer and the dissociated alpha and beta monomers has been studied by analytical ultracentrifugation with use of a new method employing short solution columns, allowing rapid equilibration and hence short runs, minimizing tubulin decay. Simultaneous analysis of ...

journal_title：Biochemistry

authors： Sackett DL,Lippoldt RE

更新日期：1991-04-09 00:00:00

abstract：:Modification of the bacterial cell wall heteropolymer peptidoglycan by addition of an acetyl group to the C-6 hydroxyl group of N-acetylmuramoyl residues is known to inhibit the activity of muramidases (lysozymes) of innate immune systems. The O-acetylation of peptidoglycan also precludes the action of intrinsic lytic...

journal_title：Biochemistry

authors： Weadge JT,Clarke AJ

更新日期：2006-01-24 00:00:00

abstract：:The highly processive motor, myosin V, has an extremely long neck containing six calmodulin-binding IQ motifs that allows it to take multiple 36 nm steps corresponding to the pseudo-repeat of actin. To further investigate how myosin V moves processively on actin filaments, we altered the length of the neck by adding o...

journal_title：Biochemistry

authors： Sakamoto T,Yildez A,Selvin PR,Sellers JR

更新日期：2005-12-13 00:00:00

abstract：:The enzyme 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase (EC 4.1. 2.16) (KDO 8-P synthase) that catalyzes the condensation of D-arabinose 5-phosphate (A 5-P) with phosphoenolpyruvate (PEP) to give 3-deoxy-D-manno-octulosonic acid 8-phosphate (KDO 8-P) and inorganic phosphate (Pi) was inactivated by the thiol-m...

journal_title：Biochemistry

authors： Salleh HM,Patel MA,Woodard RW

更新日期：1996-07-09 00:00:00

abstract：:The distance between the calcium site (site I) and the zinc site (site II) in alpha-lactalbumin was estimated from Forster energy-transfer measurements between donor Eu(III) [or Tb(III)] at site I and acceptor Co(II) at site II to be 11.5 +/- 1.5 A. Intersite distances were also measured between the bis-ANS [4,4'-bis[...

journal_title：Biochemistry

authors： Musci G,Berliner LJ

更新日期：1986-08-26 00:00:00

abstract：:Protein disulfide isomerase (PDI) utilizes the active site sequence Cys-Gly-His-Cys (CGHC; E degrees ' = -180 mV) to effect thiol-disulfide interchange during oxidative protein folding. Here, the Cys-Gly-Cys-NH(2) (CGC) peptide is shown to have a disulfide reduction potential (E degrees ' = -167 mV) that is close to t...

journal_title：Biochemistry

authors： Woycechowsky KJ,Raines RT

更新日期：2003-05-13 00:00:00

abstract：:Venomous organisms have evolved a variety of structurally diverse peptide neurotoxins that target ion channels. Despite the lack of any obvious structural homology, unrelated toxins that interact with voltage-activated K(+) channels share a dyad motif composed of a lysine and a hydrophobic amino acid residue, usually ...

journal_title：Biochemistry

authors： Al-Sabi A,Lennartz D,Ferber M,Gulyas J,Rivier JE,Olivera BM,Carlomagno T,Terlau H

更新日期：2004-07-13 00:00:00

abstract：:The molecular mass (M(r)) of the nickel- and iron-sulfur-containing enzyme CO dehydrogenase from Clostridium thermoaceticum was determined by sedimentation equilibrium ultracentrifugation to be 300,000 +/- 30,000 Da. Since the enzyme is known to contain equal numbers of two types of subunits (M(r) = 82,000 Da for alph...

journal_title：Biochemistry

authors： Xia J,Sinclair JF,Baldwin TO,Lindahl PA

更新日期：1996-02-13 00:00:00

abstract：:Complex I, a key component of the mitochondrial respiratory chain, exhibits diminished activity as a result of cardiac ischemia/reperfusion. Cardiac ischemia/reperfusion is associated with increases in the levels of mitochondrial Ca(2+) and pro-oxidants. In the current in vitro study, we sought evidence for a mechanis...

journal_title：Biochemistry

authors： Sadek HA,Szweda PA,Szweda LI

更新日期：2004-07-06 00:00:00

abstract：:Expansions of (CTG)·(CAG) repeated DNAs are the mutagenic cause of 14 neurological diseases, likely arising through the formation and processing of slipped-strand DNAs. These transient intermediates of repeat length mutations are formed by out-of-register mispairing of repeat units on complementary strands. The three-...

journal_title：Biochemistry

authors： Slean MM,Reddy K,Wu B,Nichol Edamura K,Kekis M,Nelissen FH,Aspers RL,Tessari M,Schärer OD,Wijmenga SS,Pearson CE

更新日期：2013-02-05 00:00:00

abstract：:Following ribonuclease digestion of methyl-3H-labeled B77 avian sarcoma virus RNA subunits, methylated oligonucleotides were isolated by diethylaminoethylcellulose chromotogrpahy. Partial nucleotide sequences were deduced from the known enzymatic specificities of the ribonucleases. In addition to methylated nucleoside...

journal_title：Biochemistry

authors： Dimock K,Stoltzfus CM

更新日期：1977-02-08 00:00:00

abstract：:Chemical and enzymatic probing (footprinting) of the reactivity of the promoter DNA backbone is applied to characterize two binary open complexes RPo1 (-Mg2+) and RPo2 (+Mg2+), formed by Escherichia coli RNA polymerase (E sigma 70) at the lambda PR promoter. We report that HO. detects major differences in backbone rea...

journal_title：Biochemistry

authors： Craig ML,Suh WC,Record MT Jr

更新日期：1995-12-05 00:00:00

abstract：:To understand the role of disulfide bridges in protein stability, the thermodynamic changes in the denaturation of two mutant human lysozymes lacking a disulfide bridge between Cys-77 and Cys-95 (C77A and C77/95A) were analyzed using differential scanning calorimetry (DSC). At pH 3.0 and 57 degrees C, the stabilities ...

journal_title：Biochemistry

authors： Kuroki R,Inaka K,Taniyama Y,Kidokoro S,Matsushima M,Kikuchi M,Yutani K

更新日期：1992-09-08 00:00:00

abstract：:At neutral pH, compound I of Arthromyces ramosus peroxidase (ARP) was stable and was reduced to ferric ARP without apparent formation of compound II upon titration with ascorbate or hydroquinone. In the titration experiments, compound II was seen as an intermediate only at alkaline pH. However, measuring a difference ...

journal_title：Biochemistry

authors： Farhangrazi ZS,Copeland BR,Nakayama T,Amachi T,Yamazaki I,Powers LS

更新日期：1994-05-10 00:00:00

abstract：:Recently, we synthesized the first individual β,γ-CHX-dGTP diastereomers [(R)- or (S)-CHX, where X is F or Cl] and determined their structures in ternary complexes with DNA polymerase β (pol β). We now report stereospecificity by pol β on the mixed β,γ-CHX diastereomer pairs using nuclear magnetic resonance and on the...

journal_title：Biochemistry

authors： Oertell K,Wu Y,Zakharova VM,Kashemirov BA,Shock DD,Beard WA,Wilson SH,McKenna CE,Goodman MF

更新日期：2012-10-30 00:00:00

abstract：:The stable tyrosine radical YD. (tyrosine 160 in the D2 polypeptide) in photosystem II (PSII) exhibits nonexponential electron spin-lattice relaxation transients at low temperature. As previously reported, the tetranuclear Mn complex in PSII significantly enhances the spin-lattice relaxation of YD.. However, in Mn-dep...

journal_title：Biochemistry

authors： Hirsh DJ,Beck WF,Innes JB,Brudvig GW

更新日期：1992-01-21 00:00:00

abstract：:The side chains of histidine and aspartate residues form a hydrogen bond in the active sites of many enzymes. In serine proteases, the His...Asp hydrogen bond of the catalytic triad is known to contribute greatly to catalysis, perhaps via the formation of a low-barrier hydrogen bond. In bovine pancreatic ribonuclease ...

journal_title：Biochemistry

authors： Schultz LW,Quirk DJ,Raines RT

更新日期：1998-06-23 00:00:00

abstract：:Metal cations represent key elements of RNA structure and function. In the Neurospora VS ribozyme, metal cations play diverse roles; they are important for substrate recognition, formation of the active site, and shifting the pKa's of two key nucleobases that contribute to the general acid-base mechanism. Recently, we...

journal_title：Biochemistry

authors： Bonneau E,Legault P

更新日期：2014-01-28 00:00:00

abstract：:HTC cells have been labeled by short exposures to [3H]thymidine in order to identify newly synthesized DNA. By either isolating nuclei directly or isolating them after an extensive fixation with formaldehyde, we have been able to identify two phases in the maturation process of newly replicated chromatin. The first ph...

journal_title：Biochemistry

authors： Smith PA,Jackson V,Chalkley R

更新日期：1984-03-27 00:00:00

abstract：:Pharaonis halorhodopsin (phR), the light-driven chloride ion pump from Natronobacterium pharaonis with C-terminal histidine tag, was expressed in Escherichia coli cells. The protein was solubilized with 0.1% n-dodecyl beta-D-maltopyranoside and purified with a nickel column. Removal of Cl- from the medium yields blue ...

journal_title：Biochemistry

authors： Sato M,Kanamori T,Kamo N,Demura M,Nitta K

更新日期：2002-02-19 00:00:00

abstract：:Apo-electron-transferring flavoprotein from pig kidney (apo-ETF) has been prepared by an acid ammonium sulfate procedure and reconstituted with FAD analogues to probe the flavin binding site. The 8-position of the bound flavin is accessible to solvent as judged by the reaction of 8-Cl-FAD-ETF with sodium sulfide and t...

journal_title：Biochemistry

authors： Gorelick RJ,Thorpe C

更新日期：1986-11-04 00:00:00

abstract：:We have used flash-detection optical and electron paramagnetic resonance spectroscopy to measure the kinetics and yield per flash of the photooxidation of cytochrome b559 and the yield per flash of the photooxidation of the tyrosine residue YD in Mn-depleted photosystem II (PSII) membranes at room temperature. The ini...

journal_title：Biochemistry

authors： Buser CA,Thompson LK,Diner BA,Brudvig GW

更新日期：1990-09-25 00:00:00

abstract：:The 1H resonances of the high-potential [4Fe-4S]2+ ferredoxin from Chromatium vinosum have been assigned through conventional sequential methodology applied to 2D NMR spectra. Almost 80% of the residues were identified using standard 2D COSY, HOHAHA, and NOESY pulse sequences. These residues correspond to four segment...

journal_title：Biochemistry

authors： Gaillard J,Albrand JP,Moulis JM,Wemmer DE

更新日期：1992-06-23 00:00:00

abstract：:Photosystem II, the multisubunit protein complex that oxidizes water to O2, requires the inorganic cofactors Ca2+ and Cl- to exhibit optimal activity. Chloride can be replaced functionally by a small number of anionic cofactors (Br-, NO3-, NO2-, I-), but among these anions, only Br- is capable of restoring rates of ox...

journal_title：Biochemistry

authors： Wincencjusz H,Yocum CF,van Gorkom HJ

更新日期：1999-03-23 00:00:00

abstract：:The mannitol transport protein (EIImtl) carries out translocation with concomitant phosphorylation of mannitol from the periplasm to the cytoplasm, at the expense of phosphoenolpyruvate (PEP). The phosphoryl group which is needed for this group translocation is sequentially transferred from PEP via two phosphorylation...

journal_title：Biochemistry

authors： van Weeghel RP,van der Hoek YY,Pas HH,Elferink M,Keck W,Robillard GT

更新日期：1991-02-19 00:00:00

abstract：:We introduce here a new model to describe the binding of extrinsic membrane proteins to acidic lipid membranes. In this view, macroscopic binding affinity is determined by two processes: nonspecific adsorption of protein to the membrane surface and association of acidic lipids with specific sites on the bound protein....

journal_title：Biochemistry

authors： Cutsforth GA,Whitaker RN,Hermans J,Lentz BR

更新日期：1989-09-05 00:00:00

abstract：:Type B streptogramins, such as virginiamycin S (VS), are cyclic hexadepsipeptides, inhibiting protein synthesis in prokaryotes. L-Thr connects a 3-hydroxypicolinyl residue (3-OH-Pic) to the peptide lactone ring. The fluorescence intensity of 3-OH-Pic is strongly increased by chelation to alkaline earth cations or bind...

journal_title：Biochemistry

authors： Di Giambattista M,Engelborghs Y,Nyssen E,Clays K,Cocito C

更新日期：1991-07-23 00:00:00