Step-size is determined by neck length in myosin V.

abstract：:The ErbB family of tyrosine kinase receptors is a key element in preserving cell growth homeostasis. This family is comprised of four single-transmembrane domain proteins designated ErbB-1-4. Ligand binding initiates dimerization followed by tyrosine phosphorylation and signaling, which when uncontrolled lead to cance...

journal_title：Biochemistry

authors： Bublil EM,Cohen T,Arnusch CJ,Peleg A,Pines G,Lavi S,Yarden Y,Shai Y

更新日期：2016-10-04 00:00:00

abstract：:Electron paramagnetic resonance (EPR) measurements were performed on photosystem II (PSII) membranes that were treated with 2 M NaCl to release the 17- and 23-kilodalton (kDa) polypeptides. By using 75 microM 3-(3,4-dichlorophenyl)-1,1-dimethylurea to limit the photosystem II samples to one stable charge separation in...

journal_title：Biochemistry

authors： de Paula JC,Li PM,Miller AF,Wu BW,Brudvig GW

更新日期：1986-10-21 00:00:00

abstract：:Peroxynitrite-mediated oxidative chemistry is currently the subject of intense investigation owing to the toxic side effects associated with nitric oxide overproduction. Using direct electron spin resonance spectroscopy (ESR) at 37 degrees C, we observed that in human erythrocytes peroxynitrite induced a long-lived si...

journal_title：Biochemistry

authors： Minetti M,Scorza G,Pietraforte D

更新日期：1999-02-16 00:00:00

abstract：:Mercury ions can inhibit Candida albicans phosphomannose isomerase (PMI) by two different processes at sub-micromolar concentrations. Kinetic studies show that mercury ions are in rapid equilibrium with the enzyme and cause a clear partial noncompetitive inhibition when mannose 6-phosphate is used as the substrate. Th...

journal_title：Biochemistry

authors： Wells TN,Payton MA,Proudfoot AE

更新日期：1994-06-21 00:00:00

abstract：:NADP(H) binding is essential for fast electron transfer through the flavoprotein domain of the fusion protein P450BM3. Here we characterize the interaction of NADP(H) with the oxidized and partially reduced enzyme and the effect of this interaction on the redox properties of flavin cofactors and electron transfer. Mea...

journal_title：Biochemistry

authors： Murataliev MB,Feyereisen R

更新日期：2000-10-17 00:00:00

abstract：:The acetylenic analogue of adenosine 9-(5',6'-dideoxy-beta-D-ribo-hex-5'-ynofuranosyl)adenine has been synthesized, and its behavior as an inhibitor of bovine S-adenosylhomocysteine hydrolase has been examined. Incubation of the enzyme with excess inhibitor caused a time-dependent, irreversible inactivation of the enz...

journal_title：Biochemistry

authors： Parry RJ,Muscate A,Askonas LJ

更新日期：1991-10-15 00:00:00

abstract：:Calmodulin (CaM), by mediating the stimulation of the activity of two adenylyl cyclases (ACs), plays a key role in integrating the cAMP and Ca(2+) signaling systems. These ACs, AC1 and AC8, by decoding discrete Ca(2+) signals can contribute to fine-tuning intracellular cAMP dynamics, particularly in neurons where they...

journal_title：Biochemistry

authors： Masada N,Schaks S,Jackson SE,Sinz A,Cooper DM

更新日期：2012-10-09 00:00:00

abstract：:Tropomyosins from bovine aorta and pulmonary artery exhibit identical electrophoretic patterns in sodium dodecyl sulfate but differ from tropomyosins of either chicken gizzard or rabbit skeletal muscle. Each of the four tropomyosins binds readily to skeletal muscle F-actin as indicated by their sedimentation with acti...

journal_title：Biochemistry

authors： Yamaguchi M,Ver A,Carlos A,Seidel JC

更新日期：1984-02-14 00:00:00

abstract：:The protective effect of cyclic stable nitroxide free radicals, having SOD-like activity, against oxidative damage was studied by using Escherichia coli xthA DNA repair-deficient mutant hypersensitive to H2O2. Oxidative damage induced by H2O2 was assayed by monitoring cell survival. The metal chelator 1,10-phenanthrol...

journal_title：Biochemistry

authors： Samuni A,Godinger D,Aronovitch J,Russo A,Mitchell JB

更新日期：1991-01-15 00:00:00

abstract：:As part of our studies on the molecular mechanism of mutation [Chambers, R. W. (1982) in Molecular and Cellular Mechanisms of Mutagenesis (Lemontt, J. F., & Generoso, W. M., Eds.) pp 121-145, Plenum, New York and London], we wanted to prepare specific oligonucleotides carrying O2- or O4-alkylthymidine residues. Since ...

journal_title：Biochemistry

authors： Borowy-Borowski H,Chambers RW

更新日期：1989-02-21 00:00:00

abstract：:The SH3 and SH2 domains of hematopoietic cell kinase (Hck) play important roles in substrate targeting. To identify new components of Hck signaling pathways, we identified proteins that bind to the SH3 domain of Hck (Scott et al. (2002) J. Biol. Chem. 277, 28238). One such protein was ELMO1, the mammalian orthologue o...

journal_title：Biochemistry

authors： Yokoyama N,deBakker CD,Zappacosta F,Huddleston MJ,Annan RS,Ravichandran KS,Miller WT

更新日期：2005-06-21 00:00:00

abstract：:Many lipases are potent catalysts of stereoselective reactions and are therefore of interest for use in chemical synthesis. The crystal structures of lipases show a large variation in the shapes of their active site environments that may explain the large variation in substrate specificity of these enzymes. We have de...

journal_title：Biochemistry

authors： Uppenberg J,Ohrner N,Norin M,Hult K,Kleywegt GJ,Patkar S,Waagen V,Anthonsen T,Jones TA

更新日期：1995-12-26 00:00:00

abstract：:The structure of a short-chain IgG2a antibody, which is a member of the family of mouse anti-dansyl switch variant antibodies with identical variable regions but different heavy-chain constant regions [Dangl, J.L., Parks, D. R., Oi, V. T., & Herzenberg, L. A. (1982) Cytometry 2, 395-401], is reported. Amino acid seque...

journal_title：Biochemistry

authors： Igarashi T,Sato M,Katsube Y,Takio K,Tanaka T,Nakanishi M,Arata Y

更新日期：1990-06-19 00:00:00

abstract：:Nine monoclonal antibodies were used to test calcium and EDTA effects on the molecular conformation of ventricular VLC2 within myosin. Antibody epitopes were located in six domains of VLC2 using recombinant proteins. The apparent association constants of these antibodies were measured in solution in the presence of ca...

journal_title：Biochemistry

authors： Eldin P,Cathiard AM,Anoal M,Léger J,Mornet D,Léger JJ,Cornillon B

更新日期：1994-10-25 00:00:00

abstract：:S-Glutathionylation is a redox-regulated modification that uncouples endothelial nitric oxide synthase (eNOS), switching its function from nitric oxide (NO) synthesis to (•)O2(-) generation, and serves to regulate vascular function. While in vitro or in vivo eNOS S-glutathionylation with modification of Cys689 and Cys...

journal_title：Biochemistry

authors： Chen CA,De Pascali F,Basye A,Hemann C,Zweier JL

更新日期：2013-09-24 00:00:00

abstract：:Stopped-flow kinetic measurements of the association of filipin with sterols in intact cells and isolated membranes of Mycoplasma capricolum were used to study the effects of varying the phospholipid in the membrane. The phospholipid composition and content of the membrane were varied by growing cells in an albumin-co...

journal_title：Biochemistry

authors： Clejan S,Bittman R,Rottem S

更新日期：1981-04-14 00:00:00

abstract：:Human P-glycoprotein (Pgp), a plasma membrane protein that confers multidrug resistance, functions as an ATP-dependent drug efflux pump. Pgp contains two ATP binding/utilization sites and exhibits ATPase activity that is stimulated in the presence of substrates and modulating agents. The mechanism of coupling of ATP h...

journal_title：Biochemistry

authors： Ramachandra M,Ambudkar SV,Chen D,Hrycyna CA,Dey S,Gottesman MM,Pastan I

更新日期：1998-04-07 00:00:00

abstract：:Cooperative binding of the bacteriophage lambda cI repressor dimer to specific sites of the phage operators OR and OL controls the developmental state of the phage. Cooperativity has long been thought to be mediated by self-assembly of repressor dimers to form tetramers which can bind simultaneously to adjacent operat...

journal_title：Biochemistry

authors： Rusinova E,Ross JB,Laue TM,Sowers LC,Senear DF

更新日期：1997-10-21 00:00:00

abstract：:We have validated that ligand peptides designed from antigen peptides could be used for targeting specific major histocompatibility complex class I (MHC-I) molecules on the cell surface. To design the ligand peptides, we used reported antigen peptides for each MHC-I molecule with high binding affinity. From the crysta...

journal_title：Biochemistry

authors： Sun X,Tokunaga R,Nagai Y,Miyahara R,Kishimura A,Kawakami S,Katayama Y,Mori T

更新日期：2020-12-15 00:00:00

abstract：:Kinetic studies of the effect of addition of Co2+ or Mn2+ to a highly purified dipeptidase from Ehrlich-Lettré mouse ascites tumor cells show that these metals specifically activate the hydrolyses of certain classes of dipeptides. This enzyme was previously (S. Hayman and E. K. Patterson, 1971, J. Biol. Chem. 246, 660...

journal_title：Biochemistry

authors： Patterson EK,Gatmaitan JS,Hayman S

更新日期：1975-09-23 00:00:00

abstract：:Members of the uracil-DNA glycosylase (UDG) enzyme family recognize and bind uracil, sequestering it within the binding site pocket and catalyzing the cleavage of the base from the deoxyribose, leaving an abasic site. The recognition and binding are passive and rely on innate dynamic motions of DNA wherein base pairs ...

journal_title：Biochemistry

authors： Carr CE,Khutsishvili I,Gold B,Marky LA

更新日期：2018-10-02 00:00:00

abstract：:The respiratory nitric oxide reductase (NOR) from Paracoccus denitrificans catalyzes the two-electron reduction of NO to N(2)O (2NO + 2H(+) + 2e(-) --> N(2)O + H(2)O), which is an obligatory step in the sequential reduction of nitrate to dinitrogen known as denitrification. NOR has four redox-active cofactors, namely,...

journal_title：Biochemistry

authors： Flock U,Watmough NJ,Adelroth P

更新日期：2005-08-09 00:00:00

abstract：:Genomic clones encompassing all the translated sequences, the 3' untranslated sequence, and 1 kb flanking the ATG translation initiation codon of bovine tropoelastin have been obtained and characterized by restriction enzyme analysis and extensive DNA sequencing. These analyses demonstrated that functionally distinct ...

journal_title：Biochemistry

authors： Yeh H,Anderson N,Ornstein-Goldstein N,Bashir MM,Rosenbloom JC,Abrams W,Indik Z,Yoon K,Parks W,Mecham R

更新日期：1989-03-21 00:00:00

abstract：:The one-for-one exchange of Cl(-) and HCO(3)(-) ions is catalyzed by human erythrocyte anion exchanger 1 (hAE1) through a ping-pong mechanism whereby the protein exists in two main conformations, with the single anion-binding site exposed at either the cytoplasmic (inner) side (E(i)) or the extracellular side (E(o)), ...

journal_title：Biochemistry

authors： Pal P,Lebedev D,Salim S,Knauf PA

更新日期：2006-05-23 00:00:00

abstract：:Multielectron multiproton reactions play an important role in both biological systems and chemical reactions involved in energy storage and manipulation. A key strategy employed by nature in achieving such complex chemistry is the use of proton-coupled redox steps. Cytochrome c nitrite reductase (ccNiR) catalyzes the ...

journal_title：Biochemistry

authors： Judd ET,Stein N,Pacheco AA,Elliott SJ

更新日期：2014-09-09 00:00:00

abstract：:The stereochemistry of proton transfer in the isomerization of [4 beta-2H]-5-androstene-3,17- dione (1d) to 4-androstene-3,17-dione (3) catalyzed by 3-oxo-delta 5-steroid isomerase (KSI) has been reinvestigated. In H2O, approximately 65% of the label is retained in the product (3); of this, one-third is at C-4 and two...

journal_title：Biochemistry

authors： Zawrotny ME,Hawkinson DC,Blotny G,Pollack RM

更新日期：1996-05-21 00:00:00

abstract：:In an earlier study, β³-puromycin was used for the selection of modified ribosomes, which were utilized for the incorporation of five different β-amino acids into Escherichia coli dihydrofolate reductase (DHFR). The selected ribosomes were able to incorporate structurally disparate β-amino acids into DHFR, in spite of...

journal_title：Biochemistry

authors： Maini R,Chowdhury SR,Dedkova LM,Roy B,Daskalova SM,Paul R,Chen S,Hecht SM

更新日期：2015-06-16 00:00:00

abstract：:Structural and biochemical characterization of aspartate 152 at the distal heme side of catalase-peroxidase (KatG) from Synechocystis PCC 6803 reveals an important functional role for this residue. In the wild-type protein, the side chain carboxyl group of Asp152 is 7.8 A apart from the heme iron and is hydrogen-bonde...

journal_title：Biochemistry

authors： Jakopitsch C,Auer M,Regelsberger G,Jantschko W,Furtmüller PG,Rüker F,Obinger C

更新日期：2003-05-13 00:00:00

abstract：:The three-dimensional structure of a 42-residue fragment containing the N-terminal EGF-like module of blood coagulation factor X was determined by means of 2D NMR spectroscopy and computer simulation. The spectroscopic data consisted of 370 NOE distances and 27 dihedral angle constraints. These were used to generate p...

journal_title：Biochemistry

authors： Ullner M,Selander M,Persson E,Stenflo J,Drakenberg T,Teleman O

更新日期：1992-07-07 00:00:00

abstract：:Haemadin is a 57-amino acid thrombin inhibitor from the land-living leech Haemadipsa sylvestris, whose structure has recently been determined in complex with human alpha-thrombin. Here we communicate the effect of ionic strength on the kinetics of the inhibition of human alpha-thrombin by haemadin, by using thrombin m...

journal_title：Biochemistry

authors： Richardson JL,Fuentes-Prior P,Sadler JE,Huber R,Bode W

更新日期：2002-02-26 00:00:00