Details of mannitol transport in Escherichia coli elucidated by site-specific mutagenesis and complementation of phosphorylation site mutants of the phosphoenolpyruvate-dependent mannitol-specific phosphotransferase system.

abstract：:Murine fibroblasts cultured in the presence of fibroblast growth factor-1 express relatively high levels of FR-1, a approximately 36 kDa protein related to the aldo-keto reductase superfamily [Donohue, P. J., Alberts, G. F., Hampton, B. S., Winkles, J. A. (1994) J. Biol. Chem. 269, 8604-8609]. While the crystal struct...

journal_title：Biochemistry

authors： Srivastava S,Harter TM,Chandra A,Bhatnagar A,Srivastava SK,Petrash JM

更新日期：1998-09-15 00:00:00

abstract：:The value of reducing the activity of ornithine decarboxylase (ODC), a key enzyme in the biosynthesis of polyamines, is well-appreciated. Polyamines are necessary components for cell growth, and manipulation of polyamine homeostasis may be an effective strategy for the treatment of a number of disorders, including neo...

journal_title：Biochemistry

authors： Ackermann JM,Kanugula S,Pegg AE

更新日期：2005-02-15 00:00:00

abstract：:Some aminoacyl-tRNA synthetases (AARSs) employ an editing mechanism to ensure the fidelity of protein synthesis. Leucyl-tRNA synthetase (LeuRS), isoleucyl-tRNA synthetase (IleRS), and valyl-tRNA synthetase (ValRS) share a common insertion, called the CP1 domain, which is responsible for clearing misformed products. Th...

journal_title：Biochemistry

authors： Mascarenhas AP,Martinis SA

更新日期：2008-04-22 00:00:00

abstract：:To elucidate a unique mechanism for the quinol oxidation in the Escherichia coli cytochrome bo, we applied pulse radiolysis technique to the wild-type enzyme with or without a single bound ubiquinone-8 at the high-affinity quinone binding site (Q(H)), using N-methylnicotinamide (NMA) as an electron mediator. With the ...

journal_title：Biochemistry

authors： Kobayashi K,Tagawa S,Mogi T

更新日期：2000-12-19 00:00:00

abstract：:We have synthesized Deltalac-acetogenins that are new acetogenin mimics possessing two n-alkyl tails without an alpha,beta-unsaturated gamma-lactone ring and suggested that their inhibition mechanism may be different from that of common acetogenins [Hamada et al. (2004) Biochemistry 43, 3651-3658]. To elucidate the in...

journal_title：Biochemistry

authors： Ichimaru N,Murai M,Abe M,Hamada T,Yamada Y,Makino S,Nishioka T,Makabe H,Makino A,Kobayashi T,Miyoshi H

更新日期：2005-01-18 00:00:00

abstract：:The HpxO enzyme from Klebsiella pneumoniae was recently proposed, on the basis of genetic studies, to catalyze the hydroxylation of uric acid to 5-hydroxyisourate as part of the purine catabolic pathway. Its primary sequence suggests that the HpxO catalytic activity depends on a flavin cofactor (FAD), contrasting with...

journal_title：Biochemistry

authors： O'Leary SE,Hicks KA,Ealick SE,Begley TP

更新日期：2009-04-14 00:00:00

abstract：:Site-directed mutagenesis has been used to replace alanine 305 with phenylalanine (A305F) and serine (A305S) in the active site of cytochrome P450 3A4 (CYP3A4). Enzyme kinetics for diazepam, erythromycin, nifedipine, and testosterone metabolism have been determined for both mutants and wild-type CYP3A4. The A305F muta...

journal_title：Biochemistry

authors： Fowler SM,Riley RJ,Pritchard MP,Sutcliffe MJ,Friedberg T,Wolf CR

更新日期：2000-04-18 00:00:00

abstract：:The influence of structural alterations to the estradiol-17 beta (E2) molecule on the induction of pS2 and Cathepsin D (Cath D) mRNAs has been examined by Northern analysis of RNA extracted from MCF-7 cells. Exposure of cultures to estratriene did not affect the level of expression of these estrogen-responsive genes. ...

journal_title：Biochemistry

authors： Pilat MJ,Hafner MS,Kral LG,Brooks SC

更新日期：1993-07-13 00:00:00

abstract：:The electron density map of the 3D crystal of Photosystem II from Thermosynechococcus vulcanus with a 1.9 Å resolution (PDB: 3ARC ) exhibits, in the two monomers in the asymmetric unit cell, an, until now, unidentified and uninterpreted strong difference in electron density centered at a distance of around 1.5 Å from ...

journal_title：Biochemistry

authors： Sugiura M,Koyama K,Umena Y,Kawakami K,Shen JR,Kamiya N,Boussac A

更新日期：2013-12-31 00:00:00

abstract：:The red pigments in meat products, including cooked cured ham, arise from the reaction of myoglobin with nitric oxide generated from exogenous nitrite. Since carcinogenic nitrosoamines may be generated by the treatment of meats with nitrite, the production of nitrite-free meat products is an attractive alternative. Ra...

journal_title：Biochemistry

authors： Taketani S,Ishigaki M,Mizutani A,Uebayashi M,Numata M,Ohgari Y,Kitajima S

更新日期：2007-12-25 00:00:00

abstract：:We have studied the Cu(II), Co(II), and Fe(III) complexes of the antineoplastic drug bleomycin by using electron spin--echo envelope spectroscopy. For all three complexes, nitrogen coordination of the metal ions is demonstrated. For the Cu(II)-- and Co(II)--drug complexes, we have been able to identify imidazole as a ...

journal_title：Biochemistry

authors： Burger RM,Adler AD,Horwitz SB,Mims WB,Peisach J

更新日期：1981-03-17 00:00:00

abstract：:The constant (CL) fragment of the immunoglobulin light chain contains only one intrachain disulfide bond buried in the interior of the molecule. The kinetics of reduction with dithiothreitol of the disulfide bond were studied at various concentrations of guanidine hydrochloride at pH 8.0 and 25 degrees C. It was found...

journal_title：Biochemistry

authors： Kikuchi H,Goto Y,Hamaguchi K

更新日期：1986-04-22 00:00:00

abstract：:The phosphorylation of (Na+, K+)ATPase from the electric organ of the electric eel is dependent on Mg2+. The amount of phosphoenzyme formed was increased by K+ and decreased by Na+. Kinetic analyses indicate that a ternary complex of ATPase, Pi and Mg2+ is formed prior to phosphorylation of the protein. Calorimetric s...

journal_title：Biochemistry

authors： Kuriki Y,Halsey J,Biltonen R,Racker E

更新日期：1976-11-16 00:00:00

abstract：:Two oxygenated iron-cobalt hybrid hemoglobins (Hbs), (alpha Co-O2 beta Fe-O2)2 and (alpha Fe-O2 beta Co-O2)2, were studied by electron spin echo envelope modulation (ESEEM) spectroscopy in order to measure (i) electron-nuclear hyperfine and nuclear quadrupole coupling to the N epsilon of the proximal histidyl imidazol...

journal_title：Biochemistry

authors： Lee HC,Peisach J,Tsuneshige A,Yonetani T

更新日期：1995-05-23 00:00:00

abstract：:The enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) catalyzes the reaction between shikimate 3-phosphate and phosphoenolpyruvate to form 5-enolpyruvylshikimate 3-phosphate, an intermediate in the shikimate pathway, which leads to the biosynthesis of aromatic amino acids. EPSPS exists in an open conformation...

journal_title：Biochemistry

authors： Marques MR,Vaso A,Neto JR,Fossey MA,Oliveira JS,Basso LA,dos Santos DS,de Azevedo Junior WF,Palma MS

更新日期：2008-07-15 00:00:00

abstract：:A conformational transition is described for the polypeptide, gramicidin A, in which a dimer that forms a left-handed intertwined antiparallel helix is converted to a single-stranded amino terminus to amino terminus right-handed helix. The starting structure is determined here by solution NMR methods while reference i...

journal_title：Biochemistry

authors： Zhang Z,Pascal SM,Cross TA

更新日期：1992-09-22 00:00:00

abstract：:A panel of six naïve 14-residue random peptide libraries displayed polyvalently on M13 phage was pooled and sorted against human leukemia inhibitory factor (LIF). After four rounds of selection, a single large family of peptides with the consensus sequence XCXXXXG(A/S)(D/E)(W/F)WXCF was found to bind specifically to L...

journal_title：Biochemistry

authors： Fairlie WD,Uboldi AD,Hemmings GJ,Smith BJ,Martin HM,Morgan PO,Baca M

更新日期：2003-11-18 00:00:00

abstract：:Protein L is a multidomain protein expressed at the surface of some strains of the anaerobic bacterial species Peptostreptococcus magnus. It has affinity for immunoglobulin (Ig) through interaction with framework structures in the variable Ig light chain domain. The Ig-binding activity is located to five homologous re...

journal_title：Biochemistry

authors： Wikström M,Drakenberg T,Forsén S,Sjöbring U,Björck L

更新日期：1994-11-29 00:00:00

abstract：:We mutated residue 166, located in the putative Na(+) transport pathway between transmembrane segments 4 and 5 of human Na(+)/glucose cotransporter (hSGLT1), from alanine to cysteine (A166C). A166C was expressed in Xenopus laevis oocytes, and electrophysiological methods were used to assay function. The affinity for N...

journal_title：Biochemistry

authors： Meinild AK,Loo DD,Hirayama BA,Gallardo E,Wright EM

更新日期：2001-10-02 00:00:00

abstract：:The rate of the reaction between p-nitroperoxybenzoic acid and cytochrome c peroxidase (CcP) has been investigated as a function of pH and ionic strength. The pH dependence of the reaction between CcP and peracetic acid has also been determined. The rate of the reactions are influenced by two heme-linked ionizations i...

journal_title：Biochemistry

authors： Palamakumbura AH,Foshay MC,Vitello LB,Erman JE

更新日期：1999-11-23 00:00:00

abstract：:The backbone dynamics of the liganded (holo) and unliganded (apo) forms of Escherichia coli-derived rat intestinal fatty acid-binding protein (I-FABP) have been characterized and compared using amide 15N relaxation and 1H exchange NMR measurements. The amide 1H/15N resonances for apo and holo I-FABP were assigned at 2...

journal_title：Biochemistry

authors： Hodsdon ME,Cistola DP

更新日期：1997-02-25 00:00:00

abstract：:Conformational states of the Na,K-ATPase and rates of transition between these are studied using the fluorescent dye eosin as a marker for the Na+ form (E1) and occlusion of 86Rb+ as a marker for the K+ form (E2). The aim of the present paper is to propose that the E1 form of the Na,K-ATPase can be liganded with a num...

journal_title：Biochemistry

authors： Esmann M

更新日期：1994-07-19 00:00:00

abstract：:Protein engineering to alter recognition underlying ligand binding and activity has enormous potential. Here, ligand binding for Escherichia coli phosphoenolpyruvate carboxykinase (PEPCK), which converts oxaloacetate into CO2 and phosphoenolpyruvate as the first committed step in gluconeogenesis, was engineered to acc...

journal_title：Biochemistry

authors： Tang HYH,Shin DS,Hura GL,Yang Y,Hu X,Lightstone FC,McGee MD,Padgett HS,Yannone SM,Tainer JA

更新日期：2018-12-04 00:00:00

abstract：:The helical structure of double-stranded DNA is destabilized by increasing temperature. Above a critical temperature (the melting temperature), the two strands in duplex DNA become fully separated. Below this temperature, the structural effects are localized. Using tethered particle motion in a temperature-controlled ...

journal_title：Biochemistry

authors： Driessen RP,Sitters G,Laurens N,Moolenaar GF,Wuite GJ,Goosen N,Dame RT

更新日期：2014-10-21 00:00:00

abstract：:Integrin conformational changes mediate integrin activation and signaling triggered by intracellular molecules or extracellular ligands. Even though it is known that αβ transmembrane domain separation is required for integrin signaling, it is still not clear how this signal is transmitted from the transmembrane domain...

journal_title：Biochemistry

authors： Wang W,Fu G,Luo BH

更新日期：2010-11-30 00:00:00

abstract：:The head of the P22 bacteriophage is interrupted by a unique dodecameric portal vertex that serves as a conduit for the entrance and exit of the DNA. Here, the in vitro unfolding/refolding processes of the portal protein of P22 were investigated at different temperatures (1, 25, and 37 degrees C) through the use of ur...

journal_title：Biochemistry

authors： Braga CA,Carvalho D,Lara FA,Cortines JR,Moore SD,Prevelige PE Jr,Foguel D

更新日期：2007-06-26 00:00:00

abstract：:Steroid sulfatases are responsible for the hydrolysis of 3beta-hydroxy steroid sulfates, such as cholesterol and pregnenolone sulfate, and have an important role in regulating the synthesis of estrogenic steroids, from estrone sulfate and dehydroepiandrosterone sulfate, in endocrine-dependent tumors. Although little i...

journal_title：Biochemistry

authors： Purohit A,Williams GJ,Howarth NM,Potter BV,Reed MJ

更新日期：1995-09-12 00:00:00

abstract：:Hydrogen-bonding and ionic interactions play fundamental roles in macromolecular recognition and function. In contrast to lysines and arginines, how histidines mediate these interactions is less well-understood due to the unique properties of its side chain imidazole that include an aromatic ring with two titratable n...

journal_title：Biochemistry

authors： Sepuru KM,Rajarathnam K

更新日期：2018-10-16 00:00:00

abstract：:We report a new observation of the role of Escherichia coli single-strand DNA binding protein (SSB) in synthesis of primer RNA (pRNA) catalyzed by.E.coli primase on the SSB-coated phage G4oric template. Using a set of ATP priming substrates with reactive groups attached to the 5' gamma-phosphate on different length "a...

journal_title：Biochemistry

authors： Godson GN,Mustaev AA,Sun W

更新日期：1998-03-17 00:00:00

abstract：:It had been reported previously that the beta2 subunit of Escherichia coli tryptophan synthetase [L-serinehydrolyase (adding indole) EC 4.2.1.20] can be cleaved by trypsin into a nearly functional dimeric protein, the monomer of which consists of two large, nonoverlapping, polypeptide fragments. In the present paper, ...

journal_title：Biochemistry

authors： Högberg-Raibaud A,Goldberg ME

更新日期：1977-09-06 00:00:00