Influence of Na+ on conformational states in membrane-bound renal Na,K-ATPase.

abstract：:Methylated DNA binding protein 2 (MeCP2) is a methyl CpG binding protein whose key role is the recognition of epigenetic information encoded in DNA methylation patterns. Mutation or misregulation of MeCP2 function leads to Rett syndrome as well as a variety of other autism spectrum disorders. Here, we have analyzed in...

journal_title：Biochemistry

authors： Ghosh RP,Nikitina T,Horowitz-Scherer RA,Gierasch LM,Uversky VN,Hite K,Hansen JC,Woodcock CL

更新日期：2010-05-25 00:00:00

abstract：:The structure of PsbQ, one of the three main extrinsic proteins associated with the oxygen-evolving complex (OEC) of higher plants and green algae, is examined by Fourier transform infrared (FTIR) and circular dichroic (CD) spectroscopy and by computational structural prediction methods. This protein, together with tw...

journal_title：Biochemistry

authors： Balsera M,Arellano JB,Gutiérrez JR,Heredia P,Revuelta JL,De Las Rivas J

更新日期：2003-02-04 00:00:00

abstract：:The binding of epidermal growth factor (EGF) to epidermal growth factor receptor (EGF receptor) induces dimerization of the receptor and activation of its protein tyrosine kinase. Each of these three steps was followed as a function of the concentrations of EGF and of EGF receptor. Binding of EGF was followed by sedim...

journal_title：Biochemistry

authors： Sherrill JM,Kyte J

更新日期：1996-05-07 00:00:00

abstract：:The human, cytosolic enzyme isocitrate dehydrogenase 1 (IDH1) reversibly converts isocitrate to α-ketoglutarate (αKG). Cancer-associated somatic mutations in IDH1 result in a loss of this normal function but a gain in a new or neomorphic ability to convert αKG to the oncometabolite 2-hydroxyglutarate (2HG). To improve...

journal_title：Biochemistry

authors： Rendina AR,Pietrak B,Smallwood A,Zhao H,Qi H,Quinn C,Adams ND,Concha N,Duraiswami C,Thrall SH,Sweitzer S,Schwartz B

更新日期：2013-07-02 00:00:00

abstract：:Synthetic nonviral vector systems are attractive because of their apparent simplicity of preparation and use. However, there are many barriers to success at the moment, including the formulation of uniform and reproducible particles of transfection competent, condensed nucleic acids such as plasmid DNA (pDNA). For thi...

journal_title：Biochemistry

authors： Tecle M,Preuss M,Miller AD

更新日期：2003-09-09 00:00:00

abstract：:Extended hydrogen bond networks are a common structural motif of enzymes. A recent analysis proposed quantum delocalization of protons as a feature present in the hydrogen bond network spanning a triad of tyrosines (Y(16), Y(32), and Y(57)) in the active site of ketosteroid isomerase (KSI), contributing to its unusual...

journal_title：Biochemistry

authors： Wu Y,Fried SD,Boxer SG

更新日期：2015-12-08 00:00:00

abstract：:The proton transport membrane protein bacteriorhodopsin has been biosynthetically labeled with [methyl-13C]methionine and studied by high-resolution 13C NMR after solubilization in the detergent Triton X-100. The nine methionine residues of bacteriorhodopsin give rise to four well-resolved 13C resonances, two of which...

journal_title：Biochemistry

authors： Seigneuret M,Neumann JM,Levy D,Rigaud JL

更新日期：1991-04-23 00:00:00

abstract：:G-Quadruplexes occupy important regulatory regions in the genome. DNA G-quadruplexes in the promoter regions and RNA quadruplexes in the UTRs (untranslated regions) have been individually studied and variously implicated at different regulatory levels of gene expression. However, the formation of G-quadruplexes in the...

journal_title：Biochemistry

authors： Agarwal T,Roy S,Kumar S,Chakraborty TK,Maiti S

更新日期：2014-06-17 00:00:00

abstract：:In mammals, numerous precursors of antibacterial peptides with unrelated sequences share a similar prosequence of 94-114 residues, termed the cathelin-like domain. The cathelin-like domain of protegrin-3 (ProS) was overexpressed in Escherichia coli and uniformly labeled with (15)N or (15)N and (13)C, and its three-dim...

journal_title：Biochemistry

authors： Yang Y,Sanchez JF,Strub MP,Brutscher B,Aumelas A

更新日期：2003-04-29 00:00:00

abstract：:The periplasmic nitrate reductase (NapAB), a member of the DMSO reductase superfamily, catalyzes the first step of the denitrification process in bacteria. In this heterodimer, a di-heme NapB subunit is associated to the catalytic NapA subunit that binds a [4Fe-4S] cluster and a bis(molybdopterin guanine dinucleotide)...

journal_title：Biochemistry

authors： Dementin S,Arnoux P,Frangioni B,Grosse S,Léger C,Burlat B,Guigliarelli B,Sabaty M,Pignol D

更新日期：2007-08-28 00:00:00

abstract：:Actin, an abundant cytosolic protein in eukaryotic cells, is dependent on the interaction with the chaperonin tail-less complex polypeptide 1 ring complex (TRiC) to fold to the native state. The prokaryotic chaperonin GroEL also binds non-native beta-actin, but is unable to guide beta-actin toward the native state. In...

journal_title：Biochemistry

authors： Villebeck L,Moparthi SB,Lindgren M,Hammarström P,Jonsson BH

更新日期：2007-11-06 00:00:00

abstract：:The complexity of rat liver poly (A)+ messenger RNA (mRNA) has been measured by analysis of the kinetics of hydridization with both complementary DNA (cDNA) and single copy DNA. The complementary DNA-poly(A)+ mRNA hybridization reaction demonstrates the existence of three abundance classes representing 18, 37, and 45%...

journal_title：Biochemistry

authors： Savage MJ,Sala-Trepat JM,Bonner J

更新日期：1978-02-07 00:00:00

abstract：:The crystal structure of a cytochrome P450 from the thermoacidophile Picrophilus torridus, CYP231A2 (PTO1399), has been solved. This structure reveals a wide open substrate access channel. To better understand ligand-induced structural transitions in CYP231A2, protein-ligand interactions were investigated using 4-phen...

journal_title：Biochemistry

authors： Ho WW,Li H,Nishida CR,de Montellano PR,Poulos TL

更新日期：2008-02-19 00:00:00

abstract：:We have studied the competition between Na+ and K+ for coordination by G quartets using the oligonucleotide d(G3T4G3) as a model system. d(G3T4G3) forms a dimeric foldback structure containing three G quartets in the presence of either NaCl or KCl. Proton chemical shifts, which are particular to the species of coordin...

journal_title：Biochemistry

authors： Hud NV,Smith FW,Anet FA,Feigon J

更新日期：1996-12-03 00:00:00

abstract：:Protein amyloid fibrils are originally identified as pathological entities in a variety of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. Recent studies have revealed that amyloid fibrils also serve as functional protein assemblies to fulfill a wide range of biological functions. Decip...

journal_title：Biochemistry

authors： Li D,Liu C

更新日期：2020-02-11 00:00:00

abstract：:The gel to liquid-crystalline phase transition temperatures of dispersions of mixed-acid sn-1,2-lecithins which contain one unsaturated and one saturated fatty acid have been studied by differential scanning calorimetry. The temperature for 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine (containing no reversed isome...

journal_title：Biochemistry

authors： Davis PJ,Fleming BD,Coolbear KP,Keough KM

更新日期：1981-06-09 00:00:00

abstract：:The mechanism of N-methyltryptophan oxidase, a flavin-dependent amine oxidase from Escherichia coli, was studied using a combination of kinetic isotope effects and theoretical calculations. The 15(kcat/Km) kinetic isotope effect for sarcosine oxidation is pH-dependent with a limiting value of 0.994-0.995 at high pH. D...

journal_title：Biochemistry

authors： Ralph EC,Hirschi JS,Anderson MA,Cleland WW,Singleton DA,Fitzpatrick PF

更新日期：2007-06-26 00:00:00

abstract：:The substrate specificities of two incorrectly annotated enzymes belonging to cog3964 from the amidohydrolase superfamily were determined. This group of enzymes are currently misannotated as either dihydroorotases or adenine deaminases. Atu3266 from Agrobacterium tumefaciens C58 and Oant2987 from Ochrobactrum anthropi...

journal_title：Biochemistry

authors： Ornelas A,Korczynska M,Ragumani S,Kumaran D,Narindoshvili T,Shoichet BK,Swaminathan S,Raushel FM

更新日期：2013-01-08 00:00:00

abstract：:It is well-known that the rapid flow of water into and out of cells is controlled by membrane proteins called aquaporins (AQPs). However, the mechanisms that allow cells to quickly respond to a changing osmotic environment are less well established. Using GFP-AQP fusion proteins expressed in HEK293 cells, we demonstra...

journal_title：Biochemistry

authors： Conner MT,Conner AC,Brown JE,Bill RM

更新日期：2010-02-09 00:00:00

abstract：:At neutral pH, compound I of Arthromyces ramosus peroxidase (ARP) was stable and was reduced to ferric ARP without apparent formation of compound II upon titration with ascorbate or hydroquinone. In the titration experiments, compound II was seen as an intermediate only at alkaline pH. However, measuring a difference ...

journal_title：Biochemistry

authors： Farhangrazi ZS,Copeland BR,Nakayama T,Amachi T,Yamazaki I,Powers LS

更新日期：1994-05-10 00:00:00

abstract：:Inhibition of the yeast F(0)F(1)-ATP synthase by the regulatory peptides IF1 and STF1 was studied using intact mitochondria and submitochondrial particles from wild-type cells or from mutants lacking one or both peptides. In intact mitochondria, endogenous IF1 only inhibited uncoupled ATP hydrolysis and endogenous STF...

journal_title：Biochemistry

authors： Venard R,Brèthes D,Giraud MF,Vaillier J,Velours J,Haraux F

更新日期：2003-06-24 00:00:00

abstract：:A novel single polypeptide endopeptidase of 24 kDa (24k-endopeptidase) was purified with a yield of 300-400 microg/L from conditioned medium of a bacterial strain which was identified as a new species in the genus Chryseobacterium Sp. on the basis of its 16S rDNA sequence and DNA:DNA hybridizations. The NH(2)-terminal...

journal_title：Biochemistry

authors： Lijnen HR,Van Hoef B,Ugwu F,Collen D,Roelants I

更新日期：2000-01-18 00:00:00

abstract：:A novel inhibitor of p38 mitogen-activated protein kinase (p38), CMPD1, identified by high-throughput screening, is characterized herein. Unlike the p38 inhibitors described previously, this inhibitor is substrate selective and noncompetitive with ATP. In steady-state kinetics experiments, CMPD1 was observed to preven...

journal_title：Biochemistry

authors： Davidson W,Frego L,Peet GW,Kroe RR,Labadia ME,Lukas SM,Snow RJ,Jakes S,Grygon CA,Pargellis C,Werneburg BG

更新日期：2004-09-21 00:00:00

abstract：:High-resolution 2D NMR spectra of the duplex CGCGAATTCGCG with deoxyribose sugars but with the normal phosphodiester linker replaced by an N3' --> P5' phosphoramidate (NP) group have been used to establish a solution structure for the duplex. Distance, angle, and base pair hydrogen-bonding constraints were used to ref...

journal_title：Biochemistry

authors： Ding D,Gryaznov SM,Wilson WD

更新日期：1998-09-01 00:00:00

abstract：:The solution conformation of the type I collagen alpha-1 chain N-telopeptide has been studied by CD and 1H NMR spectroscopy at 600 MHz in CD3OH/H2O (60/40 v/v) and H2O solutions. The 19 amino acids form the N-terminal end of the alpha-1 polypeptide chain. By the combined application of several two-dimensional, phase-s...

journal_title：Biochemistry

authors： Otter A,Kotovych G,Scott PG

更新日期：1989-10-03 00:00:00

abstract：:Annexin A2 is a member of the annexin family of Ca(2+)-dependent lipid binding proteins and believed to be engaged in membrane transport processes in a number of cell types. In small intestinal enterocytes, we localized annexin A2 to the brush border region, where it was found mainly on the lumenal side of the microvi...

journal_title：Biochemistry

authors： Danielsen EM,van Deurs B,Hansen GH

更新日期：2003-12-16 00:00:00

abstract：:Histone H3 arginine 2 (H3R2) is post-translationally modified in three different states by "writers" of the protein arginine methyltransferase (PRMT) family. H3R2 methylarginine isoforms include PRMT5-catalyzed monomethylation (me1) and symmetric dimethylation (me2s) and PRMT6-catalyzed me1 and asymmetric dimethylatio...

journal_title：Biochemistry

authors： Lorton BM,Harijan RK,Burgos ES,Bonanno JB,Almo SC,Shechter D

更新日期：2020-10-06 00:00:00

abstract：:We report an unusually high frequency (543 cm(-)(1)) for an Fe-CO stretching mode in the CO complex of Ascaris suum hemoglobin as compared to vertebrate hemoglobins in which the frequency of the Fe-CO mode is much lower. A second Fe-CO stretching mode in Ascaris hemoglobin is observed at 515 cm(-1). We propose that th...

journal_title：Biochemistry

authors： Das TK,Friedman JM,Kloek AP,Goldberg DE,Rousseau DL

更新日期：2000-02-01 00:00:00

abstract：:Calmodulin (CaM) is an essential, eukaryotic protein comprised of two highly homologous domains (N and C). CaM binds four calcium ions cooperatively, regulating a wide array of target proteins. A genetic screen of Paramecia by Kung [Kung, C. et al. (1992) Cell Calcium 13, 413-425] demonstrated that the domains of CaM ...

journal_title：Biochemistry

authors： VanScyoc WS,Newman RA,Sorensen BR,Shea MA

更新日期：2006-12-05 00:00:00

abstract：:Fully functional variable fragments (Fv) of D1.3, a mouse antibody directed against the hen egg lysozyme, were readily produced as hybrids (Fv-MalE) with the maltose-binding protein of Escherichia coli and purified independently of their antigen-binding properties. We used site-directed mutations of residues in the co...

journal_title：Biochemistry

authors： England P,Brégégère F,Bedouelle H

更新日期：1997-01-07 00:00:00