Kinetic study of DNA condensation by cationic peptides used in nonviral gene therapy: analogy of DNA condensation to protein folding.

abstract：:Thiol dioxygenases are mononuclear non-heme FeII-dependent metalloenzymes that initiate the oxidative catabolism of thiol-containing substrates to their respective sulfinates. Cysteine dioxygenase (CDO), the best characterized mammalian thiol dioxygenase, contains a three-histidine (3-His) coordination environment rat...

journal_title：Biochemistry

authors： Fernandez RL,Dillon SL,Stipanuk MH,Fox BG,Brunold TC

更新日期：2020-07-07 00:00:00

abstract：:Annexin A2 is a member of the annexin family of Ca(2+)-dependent lipid binding proteins and believed to be engaged in membrane transport processes in a number of cell types. In small intestinal enterocytes, we localized annexin A2 to the brush border region, where it was found mainly on the lumenal side of the microvi...

journal_title：Biochemistry

authors： Danielsen EM,van Deurs B,Hansen GH

更新日期：2003-12-16 00:00:00

abstract：:The reduction kinetics of cytochrome P-450 are known to be biphasic, with a rapid initial phase and a slower subsequent phase, both of which appear linear in semilogarithmic plots. The present report demonstrates that these biphasic reduction kinetics can be described in terms of a preequilibrium between high- and low...

journal_title：Biochemistry

authors： Backes WL,Sligar SG,Schenkman JB

更新日期：1982-03-16 00:00:00

abstract：:A rapid, high-yield procedure has been developed for the purification of HPr from the Escherichia coli phosphoenolpyruvate dependent phosphotransferase system. During this procedure, the protein copurifies with a 2500-dalton homopolysaccharide which we have identified as alpha 1-6 glucan. The results of steady-state k...

journal_title：Biochemistry

authors： Dooijewaard G,Roossien FF,Robillard GT

更新日期：1979-07-10 00:00:00

abstract：:Lipid-protein interactions in bovine rod outer segment disk membranes have been studied by using a series of eight stearic acid spin-label probes which were labeled at different carbon atom positions in the chain. In randomly oriented membrane dispersions, the electron spin resonance (ESR) spectra of the C-8, C-9, C-1...

journal_title：Biochemistry

authors： Pates RD,Marsh D

更新日期：1987-01-13 00:00:00

abstract：:We have purified and characterized a novel 30-kDa glycoprotein (gp30) with TGF alpha-like properties secreted from the estrogen receptor negative breast cancer cell line MDA-MB-231. This factor was immunoprecipitated by an anti-TGF alpha polyclonal antibody and also had TGF alpha-like biological activity, as assayed b...

journal_title：Biochemistry

authors： Lupu R,Wellstein A,Sheridan J,Ennis BW,Zugmaier G,Katz D,Lippman ME,Dickson RB

更新日期：1992-08-18 00:00:00

abstract：:Cytochrome P-450(M-1) [P-450(M-1)] is specifically expressed in adult male rat liver [Yoshioka, H., Morohashi, K., Sogawa, K., Miyata, T., Kawajiri, K., Hirose, T., Inayama, S., Fujii-Kuriyama, Y., & Omura, T. (1987) J. Biol. Chem. 262, 1706-1711]. Isolation and analysis of the gene for P-450(M-1) revealed that the co...

journal_title：Biochemistry

authors： Morishima N,Yoshioka H,Higashi Y,Sogawa K,Fujii-Kuriyama Y

更新日期：1987-12-15 00:00:00

abstract：:Adenosine A(2B) receptors, which play a role in inflammation and cancer, are of considerable interest as novel drug targets. To gain deeper insights into ligand binding and receptor activation, we exchanged amino acids predicted to be close to the binding pocket. The alanine mutants were stably expressed in CHO cells ...

journal_title：Biochemistry

authors： Thimm D,Schiedel AC,Sherbiny FF,Hinz S,Hochheiser K,Bertarelli DC,Maass A,Müller CE

更新日期：2013-01-29 00:00:00

abstract：:Aminoacyl-tRNA synthetases preserve the fidelity of decoding genetic information by accurately joining amino acids to their cognate transfer RNAs. Here, tRNA discrimination at the level of binding by Escherichia coli histidyl-tRNA synthetase is addressed by filter binding, analytical ultracentrifugation, and iodine fo...

journal_title：Biochemistry

authors： Bovee ML,Yan W,Sproat BS,Francklyn CS

更新日期：1999-10-12 00:00:00

abstract：:Previous studies of P-glycoprotein have demonstrated that its function can be modulated by phosphorylation. In the present study, inhibition of protein kinase C with calphostin C or stauroporine or prolonged treatment with the phorbol ester TPA decreased phosphorylation of P-glycoprotein, and impaired transport of vin...

journal_title：Biochemistry

authors： Bates SE,Lee JS,Dickstein B,Spolyar M,Fojo AT

更新日期：1993-09-07 00:00:00

abstract：:The flap endonuclease, FEN1, plays a critical role in DNA replication and repair. Human FEN1 exhibits both a 5' to 3' exonucleolytic and a structure-specific endonucleolytic activity. On primer-template substrates containing an unannealed 5'-tail, or flap structure, FEN1 employs a unique mechanism to cleave at the poi...

journal_title：Biochemistry

authors： Bornarth CJ,Ranalli TA,Henricksen LA,Wahl AF,Bambara RA

更新日期：1999-10-05 00:00:00

abstract：:Brief exposure of purple membrane (PM) to dilute Triton X-100 eliminates the actinic light effect on the relative amounts of fast M (Mf) and slow M (Ms) intermediates and alters the character and kinetics of the photocycle, without destroying the native BR trimers (Mukhopadhyay et al., 1994). Particular membrane lipid...

journal_title：Biochemistry

authors： Mukhopadhyay AK,Dracheva S,Bose S,Hendler RW

更新日期：1996-07-16 00:00:00

abstract：:In globular proteins, there are intermolecular hydrogen bonds between protein and water molecules, and between water molecules, which are bound with the proteins, in addition to intramolecular hydrogen bonds. To estimate the contribution of these hydrogen bonds to the conformational stability of a protein, the thermod...

journal_title：Biochemistry

authors： Takano K,Yamagata Y,Funahashi J,Hioki Y,Kuramitsu S,Yutani K

更新日期：1999-09-28 00:00:00

abstract：:Whether hydrogen bonds between side chains are energetically significant in proteins and peptides has been controversial. A method is given here for measuring these interactions in peptide helices by comparing the helix contents of peptides with 1, 2, or 3 interactions. Results are given for the glutamine--aspartate (...

journal_title：Biochemistry

authors： Huyghues-Despointes BM,Klingler TM,Baldwin RL

更新日期：1995-10-17 00:00:00

abstract：:Hemopexin is a plasma glycoprotein that has two structural domains (I and II) and binds and transports heme particularly to liver cells. Differential scanning calorimetry (DSC) studies show that hemopexin is largely stabilized by heme, which binds exclusively to domain I. The melting temperature (Tm) of heme-hemopexin...

journal_title：Biochemistry

authors： Wu ML,Morgan WT

更新日期：1993-07-20 00:00:00

abstract：:Fatty acid amide hydrolase (FAAH) is an integral membrane enzyme that degrades the fatty acid amide family of signaling lipids, including the endocannabinoid anandamide. Genetic or pharmacological inactivation of FAAH leads to analgesic, anti-inflammatory, anxiolytic, and antidepressant phenotypes in rodents without s...

journal_title：Biochemistry

authors： Ahn K,Johnson DS,Fitzgerald LR,Liimatta M,Arendse A,Stevenson T,Lund ET,Nugent RA,Nomanbhoy TK,Alexander JP,Cravatt BF

更新日期：2007-11-13 00:00:00

abstract：:In many crustaceans, changing concentrations of several low molecular weight compounds modulates hemocyanin oxygen binding, resulting in lower or higher oxygen affinities of the pigment. The nonphysiological effector caffeine and the physiological modulator urate, the latter accumulating in the hemolymph of the lobste...

journal_title：Biochemistry

authors： Menze MA,Hellmann N,Decker H,Grieshaber MK

更新日期：2005-08-02 00:00:00

abstract：:The positions, with respect to the plasma membrane, of lysine 905, contained in the peptide QRKIVE, and of lysine 1012, contained in the carboxy-terminal peptide, RPGGWVEKETYY, of ovine Na+/K(+)-transporting ATPase have been reported to be cytoplasmic and extracytoplasmic, respectively [Bayer, R. (1990) Biochemistry 2...

journal_title：Biochemistry

authors： Thibault D

更新日期：1993-03-23 00:00:00

abstract：:Oligonucleotide-directed mutagenesis in vivo was used to create destabilizing site specific changes at position 6 in iso-1-cytochrome c in Saccharomyces cerevisiae. These changes were made in combination with the stabilizing N52I substitution. The resulting proteins showed that a variety of forces and interactions are...

journal_title：Biochemistry

authors： Linske-O'Connell LI,Sherman F,McLendon G

更新日期：1995-05-30 00:00:00

abstract：:Protein kinase C-related kinases (PRKs) are serine/threonine kinases that are members of the protein kinase C superfamily and can be activated by binding to members of the Rho family of small G proteins via a Rho binding motif known as an HR1 domain. The PRKs contain three tandem HR1 domains at their N-termini. The st...

journal_title：Biochemistry

authors： Hutchinson CL,Lowe PN,McLaughlin SH,Mott HR,Owen D

更新日期：2011-04-12 00:00:00

abstract：:The level of albumin mRNA in the normal Buffalo rat liver and Morris hepatoma 7777 was compared by molecular hybridization with albumin complementary DNA (cDNA) and translational assays. Albumin mRNA was found to be 10% of the total rat liver poly(A)-containing RNA population but reduced approximately fourfold in the ...

journal_title：Biochemistry

authors： Tse TP,Morris HP,Taylor JM

更新日期：1978-07-25 00:00:00

abstract：:Kirromycin and related antibiotics inhibit protein synthesis in bacteria by acting on elongation factor Tu (EF-Tu). We have studied the effects of N-methylkirromycin (aurodox) on some molecular properties of this protein. The binding of the antibiotic causes a dramatic variation in the protein fluorescence emission sp...

journal_title：Biochemistry

authors： Balestrieri C,Giovane A,Quagliuolo L,Servillo L,Chinali G

更新日期：1989-08-22 00:00:00

abstract：:A mouse monoclonal IgG1 antibody has been generated against the soluble form of the calmodulin-dependent protein kinase type II. This antibody recognizes both the soluble and cytoskeletal forms of the enzyme, requiring Ca2+ (EC50 = 20 microM) for the interaction. Other divalent cations such as Zn2+, Mn2+, Cd2+, Co2+, ...

journal_title：Biochemistry

authors： LeVine H 3rd,Su JL,Sahyoun NE

更新日期：1988-08-23 00:00:00

abstract：:Beta-catenin phosphorylation at serine 45 (Ser45), threonine 41 (Thr41), Ser37, and Ser33 is critical for beta-catenin degradation, and regulation of beta-catenin phosphorylation is a central part of the canonical Wnt signaling pathway. Beta-catenin mutations at Ser45, Thr41, Ser37, and Ser33 perturb beta-catenin degr...

journal_title：Biochemistry

authors： Wu G,He X

更新日期：2006-04-25 00:00:00

abstract：:The Radical SAM (RS) enzyme PqqE catalyzes the first step in the biosynthesis of the bacterial cofactor pyrroloquinoline quinone, forming a new carbon-carbon bond between two side chains within the ribosomally synthesized peptide substrate PqqA. In addition to the active site RS 4Fe-4S cluster, PqqE is predicted to ha...

journal_title：Biochemistry

authors： Barr I,Stich TA,Gizzi AS,Grove TL,Bonanno JB,Latham JA,Chung T,Wilmot CM,Britt RD,Almo SC,Klinman JP

更新日期：2018-02-27 00:00:00

abstract：:A series of antisense oligonucleotides (ASOs) were synthesized and tested to define the best target sites within an RNA transcript of collagen for effective inhibition of expression. The test system consisted of mouse NIH 3T3 fibroblasts that were stably transfected with a human minigene for procollagen I so that the ...

journal_title：Biochemistry

authors： Laptev AV,Lu Z,Colige A,Prockop DJ

更新日期：1994-09-13 00:00:00

abstract：:The thermodynamics and kinetics of the subtransition L epsilon----P beta of sonicated unilamellar vesicles of 1-myristoyl-2-stearoylphosphatidylcholine (1M-2S-PC) and of 1-stearoyl-2-myristoylphosphatidylcholine (1S-2M-PC) were studied by equilibrium cooling curves and temperature-jump relaxation spectrometry with an ...

journal_title：Biochemistry

authors： Tümmler B,Herrmann U,Maass G,Eibl H

更新日期：1984-08-28 00:00:00

abstract：:13C NMR has been used to observe the equilibrium complex of [5,5-2H,5-13C]-5-aminolevulinate [( 5,5-2H,5-13C]ALA) bound to porphobilinogen (PBG) synthase (5-aminolevulinate dehydratase), a 280,000-dalton protein. [5,5-2H,5-13C]ALA (chemical shift 46.9 ppm in D2O) was prepared from [5-13C]ALA through enolization in deu...

journal_title：Biochemistry

authors： Jaffe EK,Markham GD

更新日期：1988-06-14 00:00:00

abstract：:Lipoprotein lipase (LPL) is a dimeric enzyme that is responsible for clearing triglyceride-rich lipoproteins from the blood. Although LPL plays a key role in cardiovascular health, an experimentally derived three-dimensional structure has not been determined. Such a structure would aid in understanding mutations in LP...

journal_title：Biochemistry

authors： Hayne CK,Yumerefendi H,Cao L,Gauer JW,Lafferty MJ,Kuhlman B,Erie DA,Neher SB

更新日期：2018-01-16 00:00:00

abstract：:Oligodeoxyribonucleotides 8-12 nucleotides in length whose sequences are complementary to the 5' end, the initiation codon regions, or the coding regions of rabbit globin mRNA were tested for their ability to inhibit translation in a rabbit reticulocyte lysate and in a wheat germ extract. The oligomers interact specif...

journal_title：Biochemistry

authors： Blake KR,Murakami A,Miller PS

更新日期：1985-10-22 00:00:00