当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > The carboxy terminus of sodium and potassium ion transporting ATPase is located on the cytoplasmic surface of the membrane.

The carboxy terminus of sodium and potassium ion transporting ATPase is located on the cytoplasmic surface of the membrane.

Abstract：

:The positions, with respect to the plasma membrane, of lysine 905, contained in the peptide QRKIVE, and of lysine 1012, contained in the carboxy-terminal peptide, RPGGWVEKETYY, of ovine Na+/K(+)-transporting ATPase have been reported to be cytoplasmic and extracytoplasmic, respectively [Bayer, R. (1990) Biochemistry 29, 2551-2256]. These results from our laboratory have been reexamined using an extension of the same procedure. Sealed right-side-out vesicles were modified with pyridoxal phosphate and sodium [3H]borohydride in the presence and absence of saponin or cholate. The modified alpha polypeptide was isolated and digested with the proteinase from Staphylococcus aureus strain V8 or trypsin to produce one or the other of these two peptides. These digests were passed over immunoadsorbents, identical to those used by Bayer, directed against pyroglutamylRXIVE or -ETYY. Unlike in the earlier studies, however, in the present studies the modified, radioactive peptides bound and eluted from the immunoadsorbents were submitted to HPLC, and their respective mobilities were compared to those of the synthetic peptides that had also been modified with pyridoxal phosphate. In this manner, the correct, modified peptide could be positively identified, and its specific radioactivity could be estimated. When cholate was added to sealed vesicles, prior to modification, there was at least a 3-fold increase in the incorporation of radioactivity into lysine 1012, consistent with a cytoplasmic location for this residue.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Thibault D

doi

10.1021/bi00062a012

subject

Has Abstract

pub_date

1993-03-23 00:00:00

pages

2813-21

issue

11

eissn

0006-2960

issn

1520-4995

journal_volume

32

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • The canine renal parathyroid hormone receptor is a glycoprotein: characterization and partial purification.

    abstract：:Covalent labeling of the canine renal parathyroid hormone receptor with [125I]bPTH(1-34) reveals several major binding components that display characteristics consistent with a physiologically relevant adenylate cyclase linked receptor. Through the use of the specific glycosidases neuraminidase and endoglycosidase F a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00398a044

    authors： Karpf DB,Arnaud CD,King K,Bambino T,Winer J,Nyiredy K,Nissenson RA

    更新日期：1987-12-01 00:00:00

  • Characterization of the ligand binding activities of vitronectin: interaction of vitronectin with lipids and identification of the binding domains for various ligands using recombinant domains.

    abstract：:Vitronectin is a multifunctional plasma glycoprotein which may regulate the systems related to protease cascades such as the coagulation, fibrinolysis, and complement systems as well as cell adhesion. Solid-phase assays and affinity chromatography on immobilized glycolipids indicated that vitronectin purified under de...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972247n

    authors： Yoneda A,Ogawa H,Kojima K,Matsumoto I

    更新日期：1998-05-05 00:00:00

  • Mode of Interaction of the Signal-Transducing Protein EIIA(Glc) with the Maltose ABC Transporter in the Process of Inducer Exclusion.

    abstract：:Enzyme IIA(Glc) (EIIA(Glc)) of the phosphoenolpyruvate phosphotransferase system for the uptake of glucose in Escherichia coli and Salmonella inhibits the maltose ATP-binding cassette transporter (MalE-FGK2) by interaction with the nucleotide-binding and -hydrolyzing subunit MalK, a process termed inducer exclusion. W...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00721

    authors： Wuttge S,Licht A,Timachi MH,Bordignon E,Schneider E

    更新日期：2016-09-27 00:00:00

  • The role of phenylalanine 31 in maintaining the conformational stability of ribonuclease P2 from Sulfolobus solfataricus under extreme conditions of temperature and pressure.

    abstract：:Ribonuclease P2 from the thermophilic archaebacterium Sulfolobus solfataricus is a small protein (7 kDa) with a known three-dimensional structure. Inspection of the structure and molecular dynamics simulation reveal that three aromatic residues (Phe5, Phe31, and Tyr33) from the hydrophobic core have a strong van der W...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi970467v

    authors： Mombelli E,Afshar M,Fusi P,Mariani M,Tortora P,Connelly JP,Lange R

    更新日期：1997-07-22 00:00:00

  • Essential cysteines in 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase from Escherichia coli: analysis by chemical modification and site-directed mutagenesis.

    abstract：:The enzyme 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase (EC 4.1. 2.16) (KDO 8-P synthase) that catalyzes the condensation of D-arabinose 5-phosphate (A 5-P) with phosphoenolpyruvate (PEP) to give 3-deoxy-D-manno-octulosonic acid 8-phosphate (KDO 8-P) and inorganic phosphate (Pi) was inactivated by the thiol-m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952373w

    authors： Salleh HM,Patel MA,Woodard RW

    更新日期：1996-07-09 00:00:00

  • Lysozyme degradation by the bovine multicatalytic proteinase complex (proteasome): evidence for a nonprocessive mode of degradation.

    abstract：:The multicatalytic proteinase complex (MPC, proteasome) is composed of 28 subunits organized into four rings surrounding a water-filled canal. The catalytic centers face the inner canal confining protein substrates to an enclosed space. Experimental findings obtained with MPC from archaebacteria suggest that degradati...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990826h

    authors： Wang R,Chait BT,Wolf I,Kohanski RA,Cardozo C

    更新日期：1999-11-02 00:00:00

  • Crystal structures of nitroalkane oxidase: insights into the reaction mechanism from a covalent complex of the flavoenzyme trapped during turnover.

    abstract：:Nitroalkane oxidase (NAO) from Fusarium oxysporum catalyzes the oxidation of neutral nitroalkanes to the corresponding aldehydes or ketones with the production of H(2)O(2) and nitrite. The flavoenzyme is a new member of the acyl-CoA dehydrogenase (ACAD) family, but it does not react with acyl-CoA substrates. We presen...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi051966w

    authors： Nagpal A,Valley MP,Fitzpatrick PF,Orville AM

    更新日期：2006-01-31 00:00:00

  • Functional characterization of zinc-finger motif in redox regulation of RPA-ssDNA interaction.

    abstract：:The 70-kDa subunit of eukaryotic replication protein A (RPA) contains a conserved four cysteine-type zinc-finger motif that has been implicated in regulation of DNA replication and repair. Unlike other zinc-finger proteins, RPA zinc-finger motif is not a DNA-binding component, and deletion of the zinc-finger had very ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001206f

    authors： You JS,Wang M,Lee SH

    更新日期：2000-10-24 00:00:00

  • Alamethicin incorporation in lipid bilayers: a thermodynamic study.

    abstract：:Interaction of the peptide antibiotic alamethicin with phospholipid vesicles has been monitored by changes in its circular dichroic and fluorescent properties. The data are consistent with an incorporation of the peptide in the lipid bilayer. Aggregation of alamethicin in the membrane phase is evident from a character...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00384a015

    authors： Rizzo V,Stankowski S,Schwarz G

    更新日期：1987-05-19 00:00:00

  • Mechanistic aspects of the DNA junction-resolving enzyme T7 endonuclease I.

    abstract：:The chemical mechanism of phosphodiester bond hydrolysis catalyzed by a junction-resolving enzyme has been investigated. Endonuclease I of phage T7 is a member of the nuclease superfamily of proteins that include many restriction enzymes, and the structure of the active site is very similar to that of BglI in particul...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0523254

    authors： Liu J,Déclais AC,Lilley DM

    更新日期：2006-03-28 00:00:00

  • Electron spin echo modulation and nuclear relaxation studies of staphylococcal nuclease and its metal-coordinating mutants.

    abstract：:Electron spin echo envelope modulation (ESEEM) spectroscopy has been applied to the determination of the number of water molecules coordinated to the metal in the binary complex of staphylococcal nuclease with Mn2+, to the ternary enzyme-Mn2+-3',5'-pdTp complex, and to ternary complexes of a number of mutant enzymes i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00421a010

    authors： Serpersu EH,McCracken J,Peisach J,Mildvan AS

    更新日期：1988-10-18 00:00:00

  • Solution structure of the actinorhodin polyketide synthase acyl carrier protein from Streptomyces coelicolor A3(2).

    abstract：:The solution structure of the actinorhodin acyl carrier protein (act apo-ACP) from the polyketide synthase (PKS) of Streptomyces coelicolor A3(2) has been determined using 1H NMR spectroscopy, representing the first polyketide synthase component for which detailed structural information has been obtained. Twenty-four ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi970006+

    authors： Crump MP,Crosby J,Dempsey CE,Parkinson JA,Murray M,Hopwood DA,Simpson TJ

    更新日期：1997-05-20 00:00:00

  • Purification and molecular properties of rabbit lung indolamine N-methyltransferase.

    abstract：:Indolamine N-methyltransferase (INMT) has been purified to an apparent homogeneity from rabbit lung, and some of its catalytic and physicochemical properties have been examined. The enzyme is a monomeric protein with a molecular weight of 31,500 +/- 1000, a molecular Stokes radius of 21.5 A, and a diffusion coefficien...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00535a054

    authors： Irace G,Colonna G,Camardella M,Della Pietra G,Porta R

    更新日期：1982-03-16 00:00:00

  • Structural and dynamic aspects of binding of a prototype lexitropsin to the decadeoxyribonucleotide d(CGCAATTGCG)2 deduced from high-resolution 1H NMR Studies.

    abstract：:Structural and dynamic properties of the self-complementary decadeoxyribonucleotide d(CGCAATTGCG)2 and the interaction between a prototype lexitropsin, or information-reading oligopeptide, and the decadeoxyribonucleotide are deduced by using high-resolution 1H NMR techniques. The nonexchangeable and imino proton reson...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00401a066

    authors： Lee M,Chang DK,Hartley JA,Pon RT,Krowicki K,Lown JW

    更新日期：1988-01-12 00:00:00

  • The high molecular weight nerve growth factor complex from Mastomys natalensis differs from the murine nerve growth factor complex.

    abstract：:Both the male and the female of Mastomys natalensis, an African rat, have high levels of nerve growth factor (NGF) in their submaxillary glands. Mastomys high molecular weight (HMW) NGF was purified by gel filtration and ion-exchange chromatography and was compared with HMW NGF from the male mouse submaxillary gland. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00418a007

    authors： Darling TL,Fahnestock M

    更新日期：1988-09-06 00:00:00

  • Ligand Design for Specific MHC Class I Molecules on the Cell Surface.

    abstract：:We have validated that ligand peptides designed from antigen peptides could be used for targeting specific major histocompatibility complex class I (MHC-I) molecules on the cell surface. To design the ligand peptides, we used reported antigen peptides for each MHC-I molecule with high binding affinity. From the crysta...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00735

    authors： Sun X,Tokunaga R,Nagai Y,Miyahara R,Kishimura A,Kawakami S,Katayama Y,Mori T

    更新日期：2020-12-15 00:00:00

  • Circular dichroic and kinetic differentiation of DNA binding modes of distamycin.

    abstract：:DNA binding modes of distamycin (DST) were investigated via comparative binding studies with oligomeric duplexes of the form d(GCG-X-GCG).d(CGC-Y-CGC), where Y is complementary to X and X = 4- or 5-base binding site. It was found that 1:1 and 2:1 drug-duplex complexes exhibit distinctly different circular dichroic (CD...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980950l

    authors： Chen FM,Sha F

    更新日期：1998-08-11 00:00:00

  • Sarcolipin Promotes Uncoupling of the SERCA Ca2+ Pump by Inducing a Structural Rearrangement in the Energy-Transduction Domain.

    abstract：:We have performed microsecond (μs) molecular dynamics simulation (MDS) to identify structural mechanisms for sarcolipin (SLN) uncoupling of Ca2+ transport from ATP hydrolysis for the sarcoplasmic reticulum Ca2+-ATPase (SERCA). SLN regulates muscle metabolism and energy expenditure to provide resistance against diet-in...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00728

    authors： Autry JM,Thomas DD,Espinoza-Fonseca LM

    更新日期：2016-11-08 00:00:00

  • Engineering of fibroblast growth factor: alteration of receptor binding specificity.

    abstract：:A five-residue loop structure in basic fibroblast growth factor (FGF-2) which extends from amino acid residue 118 to residue 122 was replaced, by cassette mutagenesis, with the corresponding seven-residue loop structure from the structural homologue acidic fibroblast growth factor (FGF-1) or the corresponding five-res...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00003a004

    authors： Seddon AP,Aviezer D,Li LY,Böhlen P,Yayon A

    更新日期：1995-01-24 00:00:00

  • Direct measurement of mercury(II) removal from organomercurial lyase (MerB) by tryptophan fluorescence: NmerA domain of coevolved γ-proteobacterial mercuric ion reductase (MerA) is more efficient than MerA catalytic core or glutathione .

    abstract：:Aerobic and facultative bacteria and archaea harboring mer loci exhibit resistance to the toxic effects of Hg(II) and organomercurials [RHg(I)]. In broad spectrum resistance, RHg(I) is converted to less toxic Hg(0) in the cytosol by the sequential action of organomercurial lyase (MerB: RHg(I) → RH + Hg(II)) and mercur...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100802k

    authors： Hong B,Nauss R,Harwood IM,Miller SM

    更新日期：2010-09-21 00:00:00

  • Kinetics of calcium uptake by isolated sarcoplasmic reticulum vesicles using flash photolysis of caged adenosine 5'-triphosphate.

    abstract：:The kinetics of ATP-induced Ca2+ uptake by vesicular dispersions of sarcoplasmic reticulum were determined with a time resolution of about 10 ms, depending on the temperature. Ca2+ uptake was initiated by the addition of ATP through the flash photolysis of P3-1-(2-nitrophenyl)-ethyl adenosine 5'-triphosphate utilizing...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00292a003

    authors： Pierce DH,Scarpa A,Topp MR,Blasie JK

    更新日期：1983-11-08 00:00:00

  • Pyrroloquinoline quinone biogenesis: demonstration that PqqE from Klebsiella pneumoniae is a radical S-adenosyl-L-methionine enzyme.

    abstract：:Biogenesis of pyrroloquinoline quinone (PQQ) in Klebsiella pneumoniae requires the expression of six genes (pqqA-F). One of these genes (pqqE) encodes a 43 kDa protein (PqqE) that plays a role in the initial steps in PQQ formation [Veletrop, J. S., et al. (1995) J. Bacteriol. 177, 5088-5098]. PqqE contains two highly ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900918b

    authors： Wecksler SR,Stoll S,Tran H,Magnusson OT,Wu SP,King D,Britt RD,Klinman JP

    更新日期：2009-10-27 00:00:00

  • Protein Synthesis with Ribosomes Selected for the Incorporation of β-Amino Acids.

    abstract：:In an earlier study, β³-puromycin was used for the selection of modified ribosomes, which were utilized for the incorporation of five different β-amino acids into Escherichia coli dihydrofolate reductase (DHFR). The selected ribosomes were able to incorporate structurally disparate β-amino acids into DHFR, in spite of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00389

    authors： Maini R,Chowdhury SR,Dedkova LM,Roy B,Daskalova SM,Paul R,Chen S,Hecht SM

    更新日期：2015-06-16 00:00:00

  • Hemin inhibits ubiquitin-dependent proteolysis in both a higher plant and yeast.

    abstract：:In eukaryotes, a major route for ATP-dependent protein breakdown proceeds through covalent intermediates of target proteins destined for degradation and the highly conserved, 76 amino acid protein ubiquitin. In rabbit reticulocytes, it has been shown that hemin effectively inhibits this pathway by blocking the catabol...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00409a025

    authors： Vierstra RD,Sullivan ML

    更新日期：1988-05-03 00:00:00

  • Induced fit or conformational selection? The role of the semi-closed state in the maltose binding protein.

    abstract：:A full characterization of the thermodynamic forces underlying ligand-associated conformational changes in proteins is essential for understanding and manipulating diverse biological processes, including transport, signaling, and enzymatic activity. Recent experiments on the maltose binding protein (MBP) have provided...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201481a

    authors： Bucher D,Grant BJ,McCammon JA

    更新日期：2011-12-06 00:00:00

  • Examining Reaction Specificity in PvcB, a Source of Diversity in Isonitrile-Containing Natural Products.

    abstract：:Many bacteria produce isonitrile-containing natural products that are derived from aromatic amino acids. The synthetic clusters that control biosynthesis most commonly encode two enzymes, designated PvcA and PvcB, as well as additional enzymes that direct synthesis of the natural product. The PvcA enzyme installs the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00255

    authors： Zhu J,Lippa GM,Gulick AM,Tipton PA

    更新日期：2015-04-28 00:00:00

  • Identification of a region at the N-terminus of phospholipase C-beta 3 that interacts with G protein beta gamma subunits.

    abstract：:Members of the phospholipase C-beta (PLC-beta) family of proteins are activated either by G alpha or G beta gamma subunits of heterotrimeric G proteins. To define specific regions of PLC-beta 3 that are involved in binding and activation by G beta gamma, a series of fragments of PLC-beta 3 as glutathione-S-transferase...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992021f

    authors： Barr AJ,Ali H,Haribabu B,Snyderman R,Smrcka AV

    更新日期：2000-02-22 00:00:00

  • Anomalous electrophoresis of deoxyribonucleic acid restriction fragments on polyacrylamide gels.

    abstract：:A detailed study has been made of the polyacrylamide gel electrophoresis of DNA restriction fragments obtained from two plasmids, pBR322 and p82-6B. Variables studied were molecular weight, gel concentration, temperature, and electric field strength. The retardation coefficients of the larger fragments (greater than 8...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00295a023

    authors： Stellwagen NC

    更新日期：1983-12-20 00:00:00

  • Structural determinants for plant annexin-membrane interactions.

    abstract：:The interactions of two plant annexins, annexin 24(Ca32) from Capsicum annuum and annexin Gh1 from Gossypium hirsutum, with phospholipid membranes have been characterized using liposome-based assays and adsorption to monolayers. These two plant annexins show a preference for phosphatidylserine-containing membranes and...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0516226

    authors： Dabitz N,Hu NJ,Yusof AM,Tranter N,Winter A,Daley M,Zschörnig O,Brisson A,Hofmann A

    更新日期：2005-12-13 00:00:00

  • Analysis of cooperativity observed in pH titrations of proton nuclear magnetic resonances of histidine residues of rabbit cardiac tropomyosin.

    abstract：:We have investigated in detail the cooperativity which we had previously observed in the pH titration profiles of the histidine residues of rabbit tropomyosin [Edwards, B. F. P., & Sykes, B. D. (1978) Biochemistry 17, 684]. Nonpolymerizing tropomyosin was prepared by carboxypeptidase digestion, and the titration profi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00517a037

    authors： Edwards BF,Sykes BD

    更新日期：1981-07-07 00:00:00