Functional characterization of zinc-finger motif in redox regulation of RPA-ssDNA interaction.

abstract：:We used a combination of optical and calorimetric techniques to investigate the incorporation of deoxythymidine --> deoxyuridine (dT --> dU) substitutions in the duplex and third strand of the parallel intramolecular triplex d(A(7)C(5)T(7)C(5)T(7)) (ATT). UV and differential scanning calorimetry melting experiments sh...

journal_title：Biochemistry

authors： Soto AM,Rentzeperis D,Shikiya R,Alonso M,Marky LA

更新日期：2006-03-07 00:00:00

abstract：:The solution nuclear magnetic resonance (NMR) structures and backbone (15)N dynamics of the specialized acyl carrier protein (ACP), RpAcpXL, from Rhodopseudomonas palustris, in both the apo form and holo form modified by covalent attachment of 4'-phosphopantetheine at S37, are virtually identical, monomeric, and corre...

journal_title：Biochemistry

authors： Ramelot TA,Rossi P,Forouhar F,Lee HW,Yang Y,Ni S,Unser S,Lew S,Seetharaman J,Xiao R,Acton TB,Everett JK,Prestegard JH,Hunt JF,Montelione GT,Kennedy MA

更新日期：2012-09-18 00:00:00

abstract：:Using our hemoglobin expression system in Escherichia coli, we have constructed three recombinant hemoglobins (rHbs) with amino acid substitutions located in the alpha(1)beta(1) and alpha(1)beta(2) subunit interfaces and in the distal heme pocket of the alpha-chain: rHb(alphaV96W, betaN108K), rHb(alphaL29F, alphaV96W,...

journal_title：Biochemistry

authors： Jeong ST,Ho NT,Hendrich MP,Ho C

更新日期：1999-10-05 00:00:00

abstract：:Vibrational frequencies associated with FeC and CO stretching and FeCO bending modes have been determined via resonance Raman (RR) and infrared (IR) spectroscopy for cytochrome c peroxidase (CCP) mutants prepared by site-directed mutagenesis. These include the bacterial "wild type", CCP(MI), and mutations involving gr...

journal_title：Biochemistry

authors： Smulevich G,Mauro JM,Fishel LA,English AM,Kraut J,Spiro TG

更新日期：1988-07-26 00:00:00

abstract：:Factor XIII is the terminal enzyme of the clotting cascade. A cDNA sequence encoding human placental factor XIII was expressed in Saccharomyces cerevisiae with the yeast ADH2-4c promoter. Expression levels were a strong function of the noncoding flanking DNA content of the construction. When the terminal 3'-flanking n...

journal_title：Biochemistry

authors： Bishop PD,Teller DC,Smith RA,Lasser GW,Gilbert T,Seale RL

更新日期：1990-02-20 00:00:00

abstract：:An adenosine cyclic 3',5'-monophosphate (cAMP) dependent protein kinase has recently been shown to exist in Dictyostelium discoideum and to be developmentally regulated. In this report we have followed the chromatographic behavior of both the holoenzyme and its subunits. A cAMP-dependent holoenzyme could be obtained f...

journal_title：Biochemistry

authors： Rutherford CL,Vaughan RL,Cloutier MJ,Ferris DK,Brickey DA

更新日期：1984-09-25 00:00:00

abstract：:The kinetics and thermodynamics of Al(III) exchange between aluminum citrate (AlL) and human serum transferrin were investigated in the 7.2-8.9 pH range. The C-site of human serum apotransferrin in interaction with bicarbonate removes Al(III) from Al citrate with an exchange equilibrium constant K1 = (2.0 +/- 0.6) x 1...

journal_title：Biochemistry

authors： Hémadi M,Miquel G,Kahn PH,El Hage Chahine JM

更新日期：2003-03-18 00:00:00

abstract：:The solution structure of a modified undecamer duplex containing (-)-(7R,8S,9R,10S)-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a] pyrene covalently bonded through trans ring opening at C10 of the epoxide by the N6-amino group of deoxyadenosine (dA) was studied. This diol epoxide 1 diastereomer has the benzylic ...

journal_title：Biochemistry

authors： Schurter EJ,Sayer JM,Oh-hara T,Yeh HJ,Yagi H,Luxon BA,Jerina DM,Gorenstein DG

更新日期：1995-07-18 00:00:00

abstract：:The three-dimensional structure of amyloid beta peptide (25-35), which has neurotoxic activity, in lithium dodecyl sulfate micelles was determined by two-dimensional 1H NMR spectroscopy with simulated annealing calculations. A total of 20 converged amyloid beta peptide structures were obtained on the basis of 110 expe...

journal_title：Biochemistry

authors： Kohno T,Kobayashi K,Maeda T,Sato K,Takashima A

更新日期：1996-12-17 00:00:00

abstract：:We have determined the three-dimensional structure of a non-self-complementary oligodeoxynucleotide duplex that contains a model abasic site. The duplex contains six GC base pairs plus the abasic site at the center of one strand and corresponds to an abasic frameshift. Two-dimensional NMR studies on the nonexchangeabl...

journal_title：Biochemistry

authors： Cuniasse P,Sowers LC,Eritja R,Kaplan B,Goodman MF,Cognet JA,Le Bret M,Guschlbauer W,Fazakerley GV

更新日期：1989-03-07 00:00:00

abstract：:The cardiac and skeletal muscle sarcoplasmic reticulum ryanodine receptor Ca(2+) release channels contain thiols that are potential targets of endogenously produced reactive oxygen and nitrogen intermediates. Previously, we showed that the skeletal muscle ryanodine receptor (RyR1) has O(2)-sensitive thiols; only when ...

journal_title：Biochemistry

authors： Sun J,Yamaguchi N,Xu L,Eu JP,Stamler JS,Meissner G

更新日期：2008-12-30 00:00:00

abstract：:Expression of the Tn21 mercury-resistance (mer) locus is controlled by the merR gene product, which represses mer structural gene (merTPCAD) transcription in the absence of mercuric ion [Hg(II)] and activates it in the presence of Hg(II). In vivo DNA methylation of the mer regulatory region (merOP) shows that, with or...

journal_title：Biochemistry

authors： Heltzel A,Lee IW,Totis PA,Summers AO

更新日期：1990-10-16 00:00:00

abstract：:Despite a number of studies, the formation of the Michaelis complexes between ferredoxin-NADP (+) reductases and NADP(H) eluded detailed investigations by rapid kinetic techniques because of their high formation rates. Moreover, the reversible nature of the reaction of hydride ion transfer between these enzymes and NA...

journal_title：Biochemistry

authors： Pennati A,Zanetti G,Aliverti A,Gadda G

更新日期：2008-03-18 00:00:00

abstract：:We describe a fluorescence assay that can be used to monitor helicase-catalyzed unwinding of duplex DNA continuously in real time. The assay is based on the observation that fluorescence resonance energy transfer (FRET) occurs between donor (fluorescein) and acceptor (hexachlorofluorescein) fluorophores that are in cl...

journal_title：Biochemistry

authors： Bjornson KP,Amaratunga M,Moore KJ,Lohman TM

更新日期：1994-11-29 00:00:00

abstract：:By using some mutant peptides of transcription factor Spl, phosphate backbone contacts with the DNA binding protein containing three zinc fingers have been investigated by alkylation interference, circular permutation, DNase I footprinting, and methylation protection methods. The ethylation interference analyses of Sp...

journal_title：Biochemistry

authors： Nagaoka M,Sugiura Y

更新日期：1996-07-02 00:00:00

abstract：:Probing and interrogating protein interactions that involve acyl carrier proteins (ACP's) in fatty acid synthases and polyketide synthases are critical to understanding the molecular basis for the programmed assembly of complex natural products. Here, we have used unnatural amino acid mutagenesis to site specifically ...

journal_title：Biochemistry

authors： Ye Z,Williams GJ

更新日期：2014-12-09 00:00:00

abstract：:Cyclobutane pyrimidine dimers (Pyr < > Pyr) are the major DNA photoproducts induced by the UV component of solar radiation. Photoreactivating enzyme (DNA photolyase) repairs DNA by utilizing the energy of visible light to break the cyclobutane ring of the dimer. Photolyases are monomeric proteins of 50-60 kDa with sto...

journal_title：Biochemistry

authors： Sancar A

更新日期：1994-01-11 00:00:00

abstract：:The biosynthesis of ansamycin antibiotics, including rifamycin B, involves the synthesis of an aromatic precursor, 3-amino-5-hydroxybenzoic acid (AHBA), which serves as starter for the assembly of the antibiotics' polyketide backbone. The terminal enzyme of AHBA formation, AHBA synthase, is a dimeric, pyridoxal 5'-pho...

journal_title：Biochemistry

authors： Eads JC,Beeby M,Scapin G,Yu TW,Floss HG

更新日期：1999-08-03 00:00:00

abstract：:We have investigated the effects of mixed Na+: Mg2+ ionic solutions on the stability of the nonspecific lac repressor-DNA complex. The effects of Mg2+ are simply interpreted in terms of its role as a competitor (with repressor) for DNA sites. From these studies, the binding constant of the Mg-DNA complex can be determ...

journal_title：Biochemistry

authors： Record MT Jr,deHaseth PL,Lohman TM

更新日期：1977-11-01 00:00:00

abstract：:Biochemical and immunological properties of biosynthetically radiolabeled phosphatidylcholine-(PC-) binding proteins were investigated. The PC-binding proteins were extracted from the detergent lysate of biosynthetically radiolabeled P388D1 cells by affinity chromatography on PC-Sepharose and filtered through a Sephad...

journal_title：Biochemistry

authors： Fernandez-Botran R,Suzuki T

更新日期：1985-04-09 00:00:00

abstract：:Human Monocyte Chemotactic Protein (MCP)-2 has originally been isolated from stimulated osteosarcoma cells as a chemokine coproduced with MCP-1 and MCP-3. Here, a 5'-end extended MCP-2 cDNA was cloned from a human testis cDNA library. It encoded a 76 residue MCP-2 protein, but differed from the reported bone marrow-de...

journal_title：Biochemistry

authors： Van Coillie E,Proost P,Van Aelst I,Struyf S,Polfliet M,De Meester I,Harvey DJ,Van Damme J,Opdenakker G

更新日期：1998-09-08 00:00:00

abstract：:The two disparate functions of DCoH1 (dimerization cofactor of HNF-1)/PCD (pterin-4a-carbinolamine dehydratase) are associated with a change in oligomeric state. DCoH dimers enhance the activity of the diabetes-associated transcription factor HNF-1α (hepatocyte nuclear factor-1α), while the PCD activity of DCoH1 homot...

journal_title：Biochemistry

authors： Rho H,Jones CN,Rose RB

更新日期：2010-11-30 00:00:00

abstract：:CTP synthetase [EC 6.3.4.2, UTP:ammonia ligase (ADP-forming)] from the yeast Saccharomyces cerevisiae catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to the C-4 position of UTP to form CTP. In this work, we demonstrated that CTP synthetase utilized dUTP as a substrate to synthesize dCTP. The ...

journal_title：Biochemistry

authors： Pappas A,Park TS,Carman GM

更新日期：1999-12-14 00:00:00

abstract：:Since a major function of RNA polymerase must be to bring together substrates in the optimal configuration for internucleotide bond formation, studies have been undertaken to understand the geometrical relationship of the two substrates. A model has been constructed for the geometry of interaction of two ATP molecules...

journal_title：Biochemistry

authors： Beal RB,Pillai RP,Chuknyisky PP,Levy A,Tarien E,Eichhorn GL

更新日期：1990-06-26 00:00:00

abstract：:Escherichia coli glycerol kinase (EC 2.7.1.30; ATP:glycerol 3-phosphotransferase) is a key element in a signal transduction pathway that couples expression of genes required for glycerol metabolism to the relative availability of glycerol and glucose. Its catalytic activity is inhibited by protein-protein interactions...

journal_title：Biochemistry

authors： Liu WZ,Faber R,Feese M,Remington SJ,Pettigrew DW

更新日期：1994-08-23 00:00:00

abstract：:Suberyldicholine-induced ion translocation in the millisecond time region in acetylcholine receptor rich membrane vesicles prepared from the electric organ of Electrophorus electricus was investigated in eel Ringer's solution, pH 7.0, 1 degree C. A quench-flow technique with a time resolution of 5 ms was used to measu...

journal_title：Biochemistry

authors： Pasquale EB,Takeyasu K,Udgaonkar JB,Cash DJ,Severski MC,Hess GP

更新日期：1983-12-06 00:00:00

abstract：:We have developed a system for targeting foreign DNA to hepatocytes in vitro using a soluble DNA carrier that takes advantage of receptor-mediated endocytosis to achieve internalization. The idea is based on the fact that hepatocytes possess a unique receptor that binds and internalizes galactose-terminal (asialo)glyc...

journal_title：Biochemistry

authors： Wu GY,Wu CH

更新日期：1988-02-09 00:00:00

abstract：:The thermal dependence of the structural stability of membrane-reconstituted yeast cytochrome c oxidase has been studied by using different techniques including high-sensitivity differential scanning calorimetry, differential detergent solubility thermal gel analysis, and enzyme activity measurements. For these studie...

journal_title：Biochemistry

authors： Morin PE,Diggs D,Freire E

更新日期：1990-01-23 00:00:00

abstract：:Putative domain--domain interactions of the monomeric bifunctional enzyme indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase from Escherichia coli were probed by separating the domains on the gene level and expressing them as monofunctional proteins. The engineered monofunctional enzymes were foun...

journal_title：Biochemistry

authors： Eberhard M,Tsai-Pflugfelder M,Bolewska K,Hommel U,Kirschner K

更新日期：1995-04-25 00:00:00

abstract：:The human, cytosolic enzyme isocitrate dehydrogenase 1 (IDH1) reversibly converts isocitrate to α-ketoglutarate (αKG). Cancer-associated somatic mutations in IDH1 result in a loss of this normal function but a gain in a new or neomorphic ability to convert αKG to the oncometabolite 2-hydroxyglutarate (2HG). To improve...

journal_title：Biochemistry

authors： Rendina AR,Pietrak B,Smallwood A,Zhao H,Qi H,Quinn C,Adams ND,Concha N,Duraiswami C,Thrall SH,Sweitzer S,Schwartz B

更新日期：2013-07-02 00:00:00