Effect of salt and pH on the reductive half-reaction of Mycobacterium tuberculosis FprA with NADPH.

abstract：:In the globin family, similarities in the folding mechanism have been found among different mammalian apomyoglobins (apoMb). The best-characterized intermediate of sperm whale apoMb, called I(AGH), is mainly stabilized by nativelike contacts among the A, G, and H helices involving a cluster of hydrophobic residues tha...

journal_title：Biochemistry

authors： Musto R,Bigotti MG,Travaglini-Allocatelli C,Brunori M,Cutruzzolà F

更新日期：2004-01-13 00:00:00

abstract：:Coenzyme A (CoASH) is the major low-molecular weight thiol in Staphylococcus aureus and a number of other bacteria; the crystal structure of the S. aureus coenzyme A-disulfide reductase (CoADR), which maintains the reduced intracellular state of CoASH, has recently been reported [Mallett, T.C., Wallen, J.R., Karplus, ...

journal_title：Biochemistry

authors： Nicely NI,Parsonage D,Paige C,Newton GL,Fahey RC,Leonardi R,Jackowski S,Mallett TC,Claiborne A

更新日期：2007-03-20 00:00:00

abstract：:To better understand the factors controlling the binding of apolipoprotein molecules at the surfaces of serum lipoprotein particles, the adsorption of human apolipoprotein A-I to phospholipid monolayers has been studied. The influence of lipid packing was investigated by spreading the monolayers at various initial sur...

journal_title：Biochemistry

authors： Ibdah JA,Phillips MC

更新日期：1988-09-06 00:00:00

abstract：:The mutations spectra of cis-syn, trans-syn-I, (6-4), and Dewar pyrimidone photoproducts of the TT site of AATTAA and TATTAT in the (-) strand of a heteroduplex M13 vector were obtained in an excision and photoreversal repair deficient Escherichia coli host under SOS conditions. Oligonucleotides containing site-specif...

journal_title：Biochemistry

authors： Smith CA,Wang M,Jiang N,Che L,Zhao X,Taylor JS

更新日期：1996-04-02 00:00:00

abstract：:The three-dimensional structure of the duplex formed by the association of the unnatural oligonucleotide alpha-d(TCTAAACTC) covalently linked to an acridine derivative (m5Acr) with its natural and parallel complementary sequence beta-d(AGATTTGAG) was investigated by nuclear magnetic resonance spectroscopy and constrai...

journal_title：Biochemistry

authors： Guesnet JL,Vovelle F,Thuong NT,Lancelot G

更新日期：1990-05-22 00:00:00

abstract：:The interaction of cholesterol with phospholipids has been studied with a variety of techniques; however, the possible consequences of such interactions in vivo have not been demonstrated. In this study, the cholesterol-dependent absorbance spectrum of cytochrome P-450scc was used to monitor cholesterol availability i...

journal_title：Biochemistry

authors： Stevens VL,Lambeth JD,Merrill AH Jr

更新日期：1986-07-29 00:00:00

abstract：:Specificity of the binding of sterols and related compounds with purified F-protein (fusion protein) of the HVJ (Sendai virus) was studied by binding competition with [3H]cholesterol. Requirement for cholesterol or the A/B ring trans structure and nonrequirement for the 3-hydroxyl group were found in this binding. Bin...

journal_title：Biochemistry

authors： Asano K,Asano A

更新日期：1988-02-23 00:00:00

abstract：:The cystic fibrosis transmembrane conductance regulator (CFTR) functions in vivo as a cAMP-activated chloride channel. A member of the ATP-binding cassette superfamily of membrane transporters, CFTR contains two transmembrane domains (TMDs), two nucleotide-binding domains (NBDs), and a regulatory (R) domain. It is pre...

journal_title：Biochemistry

authors： Ketchum CJ,Rajendrakumar GV,Maloney PC

更新日期：2004-02-03 00:00:00

abstract：:The interaction of bovine spleen profilin with ATP- and ADP-G-actin and poly(L-proline) has been studied by spectrofluorimetry, analytical ultracentrifugation, and rapid kinetics in low ionic strength buffer. Profilin binding to G-actin is accompanied by a large quenching of tryptophan fluorescence, allowing the measu...

journal_title：Biochemistry

authors： Perelroizen I,Marchand JB,Blanchoin L,Didry D,Carlier MF

更新日期：1994-07-19 00:00:00

abstract：:Heme (iron protoporphyrin IX) is an essential protein prosthetic group and signaling molecule required for most life on Earth. All heme-dependent processes require the dynamic and rapid mobilization of heme from sites of synthesis or uptake to hemoproteins present in virtually every subcellular compartment. The cytoto...

journal_title：Biochemistry

authors： Hanna DA,Martinez-Guzman O,Reddi AR

更新日期：2017-04-04 00:00:00

abstract：:Mdm2 negatively regulates p53 by inhibiting its transcriptional activity and promoting its degradation by functioning as an E3 ubiquitin ligase. The primary p53 binding site on mdm2 is located in its N-terminal domain. Through binding to p53 at its N-terminal transactivation domain, mdm2 directly blocks the transcript...

journal_title：Biochemistry

authors： Ma J,Martin JD,Zhang H,Auger KR,Ho TF,Kirkpatrick RB,Grooms MH,Johanson KO,Tummino PJ,Copeland RA,Lai Z

更新日期：2006-08-01 00:00:00

abstract：:Vesicular stomatitis virus buds from domains of the plasma membrane that have a unique protein and lipid composition. Fluorescence digital imaging microscopy and resonance energy transfer were used to determine how the two viral envelope-associated proteins, the G and the M proteins, could alter the lateral distributi...

journal_title：Biochemistry

authors： Luan P,Yang L,Glaser M

更新日期：1995-08-08 00:00:00

abstract：:A minor fraction of plasma high-density lipoprotein (pre beta-1 HDL) has been shown to promote cholesterol efflux from peripheral cell membranes [Castro, G. R., & Fielding, C. J. (1988) Biochemistry 27, 25-29]. When isolated native plasma is incubated at 37 degrees C, this fraction is specifically decreased. On the ot...

journal_title：Biochemistry

authors： Miida T,Kawano M,Fielding CJ,Fielding PE

更新日期：1992-11-17 00:00:00

abstract：:Inheritance of apoE4 is a strong risk factor for the development of late-onset sporadic Alzheimer's disease (AD). Several lines of evidence suggest that apoE4 binds to the Alzheimer Abeta protein and, under certain experimental conditions, promotes formation of beta-sheet structures and amyloid fibrils. Deposition of ...

journal_title：Biochemistry

authors： Poeggeler B,Miravalle L,Zagorski MG,Wisniewski T,Chyan YJ,Zhang Y,Shao H,Bryant-Thomas T,Vidal R,Frangione B,Ghiso J,Pappolla MA

更新日期：2001-12-11 00:00:00

abstract：:The recA locus of pathogenic mycobacteria differs from that of nonpathogenic species because it contains large intervening sequences nested in the RecA homology region that are excised by an unusual protein-splicing reaction. In vivo assays indicated that Mycobacterium tuberculosis recA partially complemented Escheric...

journal_title：Biochemistry

authors： Kumar RA,Vaze MB,Chandra NR,Vijayan M,Muniyappa K

更新日期：1996-02-13 00:00:00

abstract：:The addition of a small peptide can significantly increase the rate at which pepsin cleaves a substrate at pH 4.5. Why? In order to find out, we have determined spectrophotometrically the relative ability of over a dozen peptides to speed the initial rate of disappearance of Phe-Trp-NH2 and Leu-Trp-Met-Arg. Here are s...

journal_title：Biochemistry

authors： Silver MS,James SL

更新日期：1981-05-26 00:00:00

abstract：:In the ras gene superfamily, codon 12 (-TGGTG-) of the K-ras gene is the most frequently mutated codon in human cancers. Recently, we have found that bulky chemical carcinogens preferentially form DNA adducts at codons 12 and 14 (-CGTAG-) in the K-ras gene in normal human bronchial epithelial (NHBE) cells. Furthermore...

journal_title：Biochemistry

authors： Hu W,Feng Z,Tang MS

更新日期：2003-08-26 00:00:00

abstract：:N-Terminal glutaminyl and glutamyl residues of peptides and proteins tend to form pyroglutamic acid (pGlu) by intramolecular cylization. The rate constants for spontaneous cyclization of glutamine (10(-6) s(-1)) and glutamic acid (10(-9) s(-1)) in aqueous solution differ by approximately 3 orders of magnitude at pH 6....

journal_title：Biochemistry

authors： Seifert F,Schulz K,Koch B,Manhart S,Demuth HU,Schilling S

更新日期：2009-12-22 00:00:00

abstract：:In the commonly used nucleation-dependent model of protein aggregation, aggregation proceeds only after a lag phase in which the concentration of energetically unfavorable nuclei reaches a critical value. The formation of oligomeric species prior to aggregation can be difficult to detect by current spectroscopic techn...

journal_title：Biochemistry

authors： Suzuki Y,Brender JR,Soper MT,Krishnamoorthy J,Zhou Y,Ruotolo BT,Kotov NA,Ramamoorthy A,Marsh EN

更新日期：2013-03-19 00:00:00

abstract：:The N-terminal region of alpha-spectrin is responsible for its association with beta-spectrin in a heterodimer, forming functional tetramers. Non-erythroid alpha-spectrin (alphaII-spectrin) has a significantly higher association affinity for beta-spectrin than the homologous erythroid alpha-spectrin (alphaI-spectrin)....

journal_title：Biochemistry

authors： Li Q,Fung LW

更新日期：2009-01-13 00:00:00

abstract：:Lysine side chains induce conformational changes in plasminogen (Pg) that regulate the process of fibrinolysis or blood clot dissolution. A lysine side-chain mimic, epsilon amino caproic acid (EACA), enhances the activation of Pg by urinary-type and tissue-type Pg activators but inhibits Pg activation induced by strep...

journal_title：Biochemistry

authors： Lin LF,Houng A,Reed GL

更新日期：2000-04-25 00:00:00

abstract：:In this study, we have investigated the effect of hydrophobic mismatch between the thickness of the membrane and a transmembrane segment of a protein that directly inserts into the membrane bilayer. For this purpose we used mutants of the single-spanning Pf3 coat protein that can spontaneously insert into Escherichia ...

journal_title：Biochemistry

authors： Ridder AN,van de Hoef W,Stam J,Kuhn A,de Kruijff B,Killian JA

更新日期：2002-04-16 00:00:00

abstract：:The coexistence effects of multiple kinds of oxidized deoxyribonucleotides were examined using an SV40 origin-dependent in vitro replication system with a HeLa extract. Oxidized dGTP and dATP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP) and 2-hydroxy-2'-deoxyadenosine 5'-triphosphate (2-OH-dATP), were used...

journal_title：Biochemistry

authors： Satou K,Kasai H,Masutani C,Hanaoka F,Harashima H,Kamiya H

更新日期：2007-06-05 00:00:00

abstract：:Resonance Raman spectra of the photosynthetic reaction center isolated from the green bacterium Chloroflexus aurantiacus have been obtained with excitation in the near-infrared absorption bands of the special pair (P) and the accessory bacteriochlorophyll (B) using shifted-excitation Raman difference spectroscopy (SER...

journal_title：Biochemistry

authors： Cherepy NJ,Holzwarth AR,Mathies RA

更新日期：1995-04-18 00:00:00

abstract：:Phosphoprotein particles were isolated in their native state from the physiological fluid of the estuarine clam Rangia cuneata , and the characteristics of the mineral ion-protein complex which constitutes the native particle were investigated by using mineral ion binding and mineral ion exchange techniques. The parti...

journal_title：Biochemistry

authors： Marsh ME,Sass RL

更新日期：1984-03-27 00:00:00

abstract：:The glucose derivative, 2,2,6,6-tetramethylpiperidine-1-oxylglucose (TEMPO-glucose) was synthesized and examined for its ability to substitute for glucose as a substrate for the intestinal brush border membrane Na+/glucose cotransporter. TEMPO-glucose inhibited Na(+)-dependent phlorizin binding with an apparent KI of ...

journal_title：Biochemistry

authors： Peerce BE

更新日期：1991-04-30 00:00:00

abstract：:Protein L31 from the 50S ribosomal subunit of Escherichia coli was manually sequenced by the dansyl-Edman method. Owing to the availability of only small quantities of purified L31, sequencing methods were scaled down such that the entire primary structure could be determined with 700 microgram of protein. The techniq...

journal_title：Biochemistry

authors： Brosius J

更新日期：1978-02-07 00:00:00

abstract：:In the anaerobic sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough (DvH), the genome sequencing revealed the presence of three operons encoding formate dehydrogenases. fdh1 encodes an alphabetagamma trimeric enzyme containing 11 heme binding sites; fdh2 corresponds to an alphabetagamma trimeric enzyme wi...

journal_title：Biochemistry

authors： ElAntak L,Dolla A,Durand MC,Bianco P,Guerlesquin F

更新日期：2005-11-15 00:00:00

abstract：:A solubilized preparation of steroid 5 alpha-reductase (EC 1.3.1.30) from rat liver has been used in studies focused toward an understanding of the kinetic mechanism associated with enzyme catalysis. From the results of analyses with product and dead-end inhibitors, a preferentially ordered binding of substrates and r...

journal_title：Biochemistry

authors： Levy MA,Brandt M,Greway AT

更新日期：1990-03-20 00:00:00

abstract：:The mechanism of the argininosuccinate lyase reaction has been probed by the measurement of the effects of isotopic substitution at the reaction centers. A primary deuterium isotope effect of 1.0 on both V and V/K is obtained with (2S,3R)-argininosuccinate-3-d, while a primary 15N isotope effect on V/K of 0.9964 +/- 0...

journal_title：Biochemistry

authors： Kim SC,Raushel FM

更新日期：1986-08-26 00:00:00