Sarcolipin Promotes Uncoupling of the SERCA Ca2+ Pump by Inducing a Structural Rearrangement in the Energy-Transduction Domain.

abstract：:Porcine aortic myosin is a smooth muscle contractile protein similar to its striated muscle counterpart. Electrophoresis in sodium dodecyl sulfate indicates that the molecule consists of three classes of subunits with polypeptide chain molecular weights of 192,000, 19,000, and 15,000. At 277 nm the absorption spectrum...

journal_title：Biochemistry

authors： Frederiksen DW

更新日期：1979-05-01 00:00:00

abstract：:Neuropeptide Y (NPY) and the pancreatic polypeptide (PP) are members of the neuropeptide Y family of hormones. They bind to the Y receptors with very different affinities: Whereas PP is highly selective for the Y(4) receptor, NPY displays highest affinites for Y(1), Y(2), and Y(5) receptor subtypes. Introducing the NP...

journal_title：Biochemistry

authors： Lerch M,Kamimori H,Folkers G,Aguilar MI,Beck-Sickinger AG,Zerbe O

更新日期：2005-06-28 00:00:00

abstract：:The present paper reports a study on the equilibria and kinetics of the acid-alkaline transition and the azide binding reaction by ferric Aplysia myoglobin. A single completely reversible spectrophotometric titration curve is found over the pH range from similar to 5 to similar to 9, with an apparent pK equals to 7.5 ...

journal_title：Biochemistry

authors： Giacometti GM,Da Ros A,Antonini E,Brunori M

更新日期：1975-04-22 00:00:00

abstract：:The transcriptional activity of the hexafluorinated derivative of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], 26,26,26,27,27,27-hexafluoro-1,25-dihydroxyvitamin D3 [F6-1,25-(OH)2D3], was examined in cultured cells by a transient expression assay (CAT assay) using expression vectors for the rat nuclear vitamin D3 receptor...

journal_title：Biochemistry

authors： Sasaki H,Harada H,Handa Y,Morino H,Suzawa M,Shimpo E,Katsumata T,Masuhiro Y,Matsuda K,Ebihara K

更新日期：1995-01-10 00:00:00

abstract：:The retaining beta-1,4-glycanase Cex from Cellulomonas fimi, a family 10 glycosyl hydrolase, hydrolyzes xylan 40-fold more efficiently than cellulose. To gain insight into the nature of its preference for xylan, we determined the crystal structure of the Cex catalytic domain (Cex-cd) trapped as its covalent 2-deoxy-2-...

journal_title：Biochemistry

authors： Notenboom V,Birsan C,Warren RA,Withers SG,Rose DR

更新日期：1998-04-07 00:00:00

abstract：:The interaction of bee venom melittin with dimyristolphosphatidylcholine (DMPC) selectively deuteriated in the choline head group has been studied by deuterium and phosphorus-31 nuclear magnetic resonance (NMR) spectroscopy. The action of residual phospholipase A2 in melittin samples resulted in mixtures of DMPC and i...

journal_title：Biochemistry

authors： Dempsey CE,Watts A

更新日期：1987-09-08 00:00:00

abstract：:Telomerase is a ribonucleoprotein that participates in the maintenance of telomere length. Its activity is up-regulated in many tumor types, suggesting that it may be a novel target for chemotherapy. The RNA component of telomerase contains an active site that plays at least two roles&sbd;binding telomere ends and tem...

journal_title：Biochemistry

authors： Hamilton SE,Pitts AE,Katipally RR,Jia X,Rutter JP,Davies BA,Shay JW,Wright WE,Corey DR

更新日期：1997-09-30 00:00:00

abstract：:We report an unusually high frequency (543 cm(-)(1)) for an Fe-CO stretching mode in the CO complex of Ascaris suum hemoglobin as compared to vertebrate hemoglobins in which the frequency of the Fe-CO mode is much lower. A second Fe-CO stretching mode in Ascaris hemoglobin is observed at 515 cm(-1). We propose that th...

journal_title：Biochemistry

authors： Das TK,Friedman JM,Kloek AP,Goldberg DE,Rousseau DL

更新日期：2000-02-01 00:00:00

abstract：:We have developed an assay to continuously monitor the branched amino acid preferring peptidase (BrAAP) activity of the proteasome. This assay is based on the hydrolysis of the fluorogenic peptide, Abz-Gly-Pro-Ala-Leu-Ala-Nba (Abz is 2-aminobenzoyl and Nba is 4-nitrobenzylamide) which is cleaved exclusively at the Leu...

journal_title：Biochemistry

authors： McCormack TA,Cruikshank AA,Grenier L,Melandri FD,Nunes SL,Plamondon L,Stein RL,Dick LR

更新日期：1998-05-26 00:00:00

abstract：:The interaction of bleomycin A2 with DNA has been examined by fluorescence spectroscopy and proton magnetic resonance techniques. Fluorescence bands observed at 353 and 405 nm in the spectrum of bleomycin were assigned to the bithiazole and 4-aminopyrimidine rings, respectively. Quenching of bithiazole fluorescence by...

journal_title：Biochemistry

authors： Chien M,Grollman AP,Horwitz SB

更新日期：1977-08-09 00:00:00

abstract：:A solubilized preparation of steroid 5 alpha-reductase (EC 1.3.1.30) from rat liver has been used in studies focused toward an understanding of the kinetic mechanism associated with enzyme catalysis. From the results of analyses with product and dead-end inhibitors, a preferentially ordered binding of substrates and r...

journal_title：Biochemistry

authors： Levy MA,Brandt M,Greway AT

更新日期：1990-03-20 00:00:00

abstract：:Cumene hydroperoxide-mediated (CuOOH-mediated) inactivation of cytochromes P450 (CYPs) results in destruction of their prosthetic heme to reactive fragments that irreversibly bind to the protein. We have attempted to characterize this process structurally, using purified, 14C-heme labeled, recombinant human liver P450...

journal_title：Biochemistry

authors： He K,Bornheim LM,Falick AM,Maltby D,Yin H,Correia MA

更新日期：1998-12-15 00:00:00

abstract：:The structure of an active mutant of (S)-mandelate dehydrogenase (MDH-GOX2) from Pseudomonas putida has been determined at 2.15 A resolution. The membrane-associated flavoenzyme (S)-mandelate dehydrogenase (MDH) catalyzes the oxidation of (S)-mandelate to give a flavin hydroquinone intermediate which is subsequently r...

journal_title：Biochemistry

authors： Sukumar N,Xu Y,Gatti DL,Mitra B,Mathews FS

更新日期：2001-08-21 00:00:00

abstract：:A series of monoclonal antibodies specific for distinguishable epitopes in chromosomal protein histone H5 were obtained from mice immunized with either free H5 or H5 . RNA complexes. The antibodies elicited by H5 could be distinguished from those elicited by H5 . RNA by their binding to native or acid-denatured H5, by...

journal_title：Biochemistry

authors： Mendelson E,Bustin M

更新日期：1984-07-17 00:00:00

abstract：:The affinity of various carbohydrates for the galactose/N-acetylgalactosamine-specific lectin of the rabbit liver was assessed by determining the effect of these carbohydrates on the binding of [125I]asialoorosomucoid (125I-ASOR) by the lectin. To obtain the concentration of the inhibitor that causes 50% reduction in ...

journal_title：Biochemistry

authors： Lee RT,Myers RW,Lee YC

更新日期：1982-11-23 00:00:00

abstract：:The pH dependence of the transient aerobic kinetics of cytochromes c and a has been investigated with cytochrome oxidase reconstituted in phospholipid vesicles in the absence and presence of an uncoupler and an ionophore. The cytochrome a reduction level immediately after the burst phase was 60-80% and was not signifi...

journal_title：Biochemistry

authors： Maison-Peteri B,Malmström BG

更新日期：1989-04-18 00:00:00

abstract：:Phospholipase A(2) (PLA(2)) enzymes become activated by binding to biological membranes and hydrolyze phospholipids to free fatty acids and lyso-phospholipids, the precursors of inflammatory mediators. To understand the functional significance of amino acid residues at key positions, we have studied the effects of the...

journal_title：Biochemistry

authors： Nemec KN,Pande AH,Qin S,Bieber Urbauer RJ,Tan S,Moe D,Tatulian SA

更新日期：2006-10-17 00:00:00

abstract：:The carbon-13 nuclear magnetic resonance (13C NMR) spectra of luteinizing hormone-releasing hormone (LH-RH) and lower homologous peptides have been assigned in aqueous solutions at various pH values. 13C spin-lattice relaxation times (T1) have been measured for all proton-bearing carbons at 25.2 and 67.9 MHz. From the...

journal_title：Biochemistry

authors： Deslauriers R,Levy GC,McGregor WH,Sarantakis K,Smith IC

更新日期：1975-09-23 00:00:00

abstract：:The prothrombinase complex is comprised of an enzyme, factor Xa, and a cofactor, factor Va, that each bind peripherally to membranes containing phosphatidylserine (PS) and activate the substrate, prothrombin. The mechanism by which the membrane contributes to enhanced catalytic efficacy of prothrombinase is not precis...

journal_title：Biochemistry

authors： Stone MD,Nelsestuen GL

更新日期：2005-03-15 00:00:00

abstract：:The relationship between cholesterol and ubiquinone synthesis in rat intestinal epithelial cell cultures was examined by using 3 beta-[2-(diethylamino)ethoxy]androst-5-en-17-one hydrochloride (U18666A). Addition of U18666A to cells caused a greater than 90% inhibition of incorporation of [3H]acetate into cholesterol a...

journal_title：Biochemistry

authors： Sexton RC,Panini SR,Azran F,Rudney H

更新日期：1983-12-06 00:00:00

abstract：:In investigating the agonist binding site of the human brain cholecystokininB receptor (CCKBR), we employed the direct protein chemical approach using a photoreactive tritiated analogue of sulfated cholecystokinin octapeptide, which contains the p-benzoylbenzoyl moiety at the N-terminus, followed by purification of th...

journal_title：Biochemistry

authors： Anders J,Blüggel M,Meyer HE,Kühne R,ter Laak AM,Kojro E,Fahrenholz F

更新日期：1999-05-11 00:00:00

abstract：:Hydrolysis of monodisperse short chain phosphatidylcholines, far below their critical micelle concentration, by phospholipase A2 (PLA2) and other interfacial enzymes is characterized. Results show that virtually all the observed hydrolysis by pancreatic and human inflammatory PLA2 occurs on surfaces of the reaction ve...

journal_title：Biochemistry

authors： Yu BZ,Berg OG,Jain MK

更新日期：1999-08-10 00:00:00

abstract：:Trypsin and N-[3-[4-[4-(amidinophenoxy)carbonyl]phenyl]-2-methyl-2-propenoyl]- N-allylglycine methanesulfonate (1), a newly designed and orally active synthetic trypsin inhibitor, were cocrystallized. The space group of the crystal is P2(1)2(1)2(1) with cell constants a = 63.74 A, b = 63.08 A, and c = 69.38 A, which i...

journal_title：Biochemistry

authors： Odagaki Y,Nakai H,Senokuchi K,Kawamura M,Hamanaka N,Nakamura M,Tomoo K,Ishida T

更新日期：1995-10-03 00:00:00

abstract：:C4'-oxidized (C4-AP) and C5'-oxidized abasic sites (DOB) that are produced following abstraction of a hydrogen atom from the DNA backbone reversibly form cross-links selectively with dA opposite a 3'-adjacent nucleotide, despite the comparable proximity of an opposing dA. A previous report on UvrABC incision of DNA su...

journal_title：Biochemistry

authors： Ghosh S,Greenberg MM

更新日期：2015-10-13 00:00:00

abstract：:During cellular uptake of fluorescently labeled cell-penetrating peptides (CPPs), intense fluorescent signals are commonly observed in the nucleus of the cell, suggesting intracellular CPP relocation and potential binding to the genome of the host. We therefore investigated the interaction of the CPP HIV-1 Tat(47-57) ...

journal_title：Biochemistry

authors： Ziegler A,Seelig J

更新日期：2007-07-10 00:00:00

abstract：:The synthesis of activated sulfate (adenosine 5'-phosphosulfate, APS) and inorganic pyrophosphate from ATP and SO4 is remarkably unfavorable: K(eq) approximately 10(-8) under presumed, near-physiological conditions. Consequently, ATP sulfurylases, which catalyze APS synthesis, suffer approximately 10(8)-fold losses in...

journal_title：Biochemistry

authors： Sun M,Leyh TS

更新日期：2006-09-26 00:00:00

abstract：:Recognition of and binding to cholesterol on the host cell membrane is an initial step in the mechanism of numerous pathogens, including viruses, bacteria, and bacterial toxins; however, a viable method of inhibiting this interaction has not yet been uncovered. Here, we describe the mechanism by which a cholesterol re...

journal_title：Biochemistry

authors： Koufos E,Chang EH,Rasti ES,Krueger E,Brown AC

更新日期：2016-08-30 00:00:00

abstract：:The specific rate of superoxide (O2(•-)) production in the purified active crystallizable cytochrome b6f complex, normalized to the rate of electron transport, has been found to be more than an order of magnitude greater than that measured in isolated yeast respiratory bc1 complex. The biochemical and structural basis...

journal_title：Biochemistry

authors： Baniulis D,Hasan SS,Stofleth JT,Cramer WA

更新日期：2013-12-17 00:00:00

abstract：:The relative roles of insulin and glucose in the regulation of hepatic glycogen synthase and phosphorylase were studied in hepatocytes from fed rats. Elevation of extra-cellular glucose led to a rapid decrease in phosphorylase a activity followed by a slower increase in glycogen synthase I activity. A reciprocal and c...

journal_title：Biochemistry

authors： Witters LA,Avruch J

更新日期：1978-02-07 00:00:00

abstract：:The aggregation of the microtubule-associated protein, tau, into amyloid fibrils is a hallmark of neurodegenerative diseases such as the tauopathies and Alzheimer's disease. Since monomeric tau is an intrinsically disordered protein and the polymeric fibrils possess an ordered cross-β core, the aggregation process is ...

journal_title：Biochemistry

authors： Ramachandran G,Milán-Garcés EA,Udgaonkar JB,Puranik M

更新日期：2014-10-21 00:00:00