Insulin regulation of hepatic glycogen synthase and phosphorylase.

abstract：:The structural underpinnings of enzyme substrate specificity are investigated in a pair of copper amine oxidases (CAOs) from Hansenula polymorpha (HPAO-1 and HPAO-2). The X-ray crystal structure (to 2.0 A resolution) and steady state kinetic data of the second copper amine oxidase (HPAO-2) are presented for comparison...

journal_title：Biochemistry

authors： Chang CM,Klema VJ,Johnson BJ,Mure M,Klinman JP,Wilmot CM

更新日期：2010-03-23 00:00:00

abstract：:Epigallocatechin-3-gallate (EGCG) is a catechin found in green tea that can inhibit the amyloid formation of a wide variety of proteins. EGCG's ability to prevent or redirect the amyloid formation of so many proteins may reflect a common mechanism of action, and thus, greater molecular-level insight into how it exerts...

journal_title：Biochemistry

authors： Marcinko TM,Drews T,Liu T,Vachet RW

更新日期：2020-03-17 00:00:00

abstract：:Cyanobacteriochromes (CBCRs) are cyanobacterial photoreceptors distantly related to phytochromes. Like phytochromes, CBCRs photointerconvert between two photostates that accompany photoisomerization of their bilin chromophores. While phytochromes typically exhibit red/far-red photocycles, CBCR photocycles are much mor...

journal_title：Biochemistry

authors： Gottlieb SM,Kim PW,Corley SC,Madsen D,Hanke SJ,Chang CW,Rockwell NC,Martin SS,Lagarias JC,Larsen DS

更新日期：2014-02-18 00:00:00

abstract：:Phosphofructokinase from Escherichia coli (EcPFK) is a homotetramer with four active sites and four allosteric sites. Understanding the allosteric activation of EcPFK by MgADP has been complicated by the complex web of possible interactions, including active site homotropic interactions, allosteric site homotropic int...

journal_title：Biochemistry

authors： Fenton AW,Paricharttanakul NM,Reinhart GD

更新日期：2004-11-09 00:00:00

abstract：:The binding modes of four active site-directed, acylating inhibitors of human alpha-thrombin have been determined using X-ray crystallography. These inhibitors (GR157368, GR166081, GR167088, and GR179849) are representatives of a series utilizing a novel 5, 5-trans-lactone template to specifically acylate Ser195 of th...

journal_title：Biochemistry

authors： Jhoti H,Cleasby A,Reid S,Thomas PJ,Weir M,Wonacott A

更新日期：1999-06-22 00:00:00

abstract：:ALY is a ubiquitously expressed nuclear protein which interacts with proteins such as TAP that are involved in export of mRNA from the nucleus to the cytoplasm, as well as with proteins that bind the T cell receptor alpha gene enhancer. ALY has also been shown to bind mRNA and to co-localize in the nucleus with compon...

journal_title：Biochemistry

authors： Pérez-Alvarado GC,Martínez-Yamout M,Allen MM,Grosschedl R,Dyson HJ,Wright PE

更新日期：2003-06-24 00:00:00

abstract：:Mutation of Pro94 to phenylalanine or alanine significantly alters the redox properties of the type I copper center of amicyanin. Each mutation increases the redox midpoint potential (E(m)) value by at least 140 mV and shifts the pK(a) for the pH dependence of the E(m) value to a more acidic value. Atomic resolution (...

journal_title：Biochemistry

authors： Carrell CJ,Sun D,Jiang S,Davidson VL,Mathews FS

更新日期：2004-07-27 00:00:00

abstract：:The properties of the nucleotide binding sites in the isolated beta and alpha subunits of H(+)-ATPase from Bacillus PS3 (TF1) have been examined by studying the EPR properties of bound VO(2+), which is a paramagnetic probe for the native Mg2+ cation cofactor. The amino acid ligands of the VO2+ complexes with the isola...

journal_title：Biochemistry

authors： Zimmermann JL,Amano T,Sigalat C

更新日期：1999-11-16 00:00:00

abstract：:In an attempt to understand the mechanism by which transcription factors compete with histone octamers for cognate binding sites in chromatin, the effect of the histone binding protein nucleoplasmin on the binding of TFIIIA to nucleosomal 5S rRNA genes was tested. In this study, it was shown that, despite the previous...

journal_title：Biochemistry

authors： Howe L,Itoh T,Katagiri C,Ausio J

更新日期：1998-02-03 00:00:00

abstract：:The tRNA(His) guanylyltransferase (Thg1) catalyzes the incorporation of a single guanosine residue at the -1 position (G(-1)) of tRNA(His), using an unusual 3'-5' nucleotidyl transfer reaction. Thg1 and Thg1 orthologs known as Thg1-like proteins (TLPs), which catalyze tRNA repair and editing, are the only known enzyme...

journal_title：Biochemistry

authors： Smith BA,Jackman JE

更新日期：2012-01-10 00:00:00

abstract：:Hydrogenase from Chromatium vinosum contains 1 Ni, 11-12 Fe, and ca. 9 sulfides. EPR and Mössbauer studies of the enzyme prepared in four different oxidation states show that the enzyme contains two Fe4S4 and one Fe3S4 cluster. In the oxidized (2+) state, the Mössbauer parameters of the two Fe4S4 clusters are typical ...

journal_title：Biochemistry

authors： Surerus KK,Chen M,van der Zwaan JW,Rusnak FM,Kolk M,Duin EC,Albracht SP,Münck E

更新日期：1994-04-26 00:00:00

abstract：:Catalysis in protein tyrosine phosphatases (PTPs) involves movement of a protein loop called the WPD loop that brings a conserved aspartic acid into the active site to function as a general acid. Mutation of the tryptophan in the WPD loop of the PTP YopH to any other residue with a planar, aromatic side chain (phenyla...

journal_title：Biochemistry

authors： Moise G,Gallup NM,Alexandrova AN,Hengge AC,Johnson SJ

更新日期：2015-10-27 00:00:00

abstract：:A soluble extract prepared from cells of an Escherichia coli strain carrying the antibiotic resistance plasmid R6K is capable of carrying out the complete process of R6K DNA replication. DNA synthesis in vitro is dependent on the four deoxyribo- and ribonucleotide triphosphates and is sensitive to rifampin and strepto...

journal_title：Biochemistry

authors： Inuzuka M,Helinski DR

更新日期：1978-06-27 00:00:00

abstract：:The Type I homodimeric photosynthetic reaction center found in anaerobic gram-positive bacteria of the genus Heliobacteriaceae incorporates FA- and FB-like iron-sulfur clusters similar to those found in Photosystem I as terminal electron acceptors. We recently isolated the PshB protein that harbors the iron-sulfur clu...

journal_title：Biochemistry

authors： Heinnickel M,Shen G,Golbeck JH

更新日期：2007-03-06 00:00:00

abstract：:We report differences in the interaction of two structurally similar phenanthroline intercalators, ethidium and propidium, with poly(dA).poly(dT) and poly[d(A-T)] as a function of ionic strength based on titration microcalorimetry, fluorescence titration, and hydrostatic pressure measurements. Both ethidium and propid...

journal_title：Biochemistry

authors： Marky LA,Macgregor RB Jr

更新日期：1990-05-22 00:00:00

abstract：:The bifunctional reagent p-[(bromoacetyl)-amino]phenyl arsenoxide (BrCH2CONHPhAsO) in the presence of excess reduced nicotinamide adenine dinucleotide has been shown to cause the irreversible active site directed inactivation of the lipoamide dehydrogenase (E3) component of the pyruvate dehydrogenase multienzyme (PD) ...

journal_title：Biochemistry

authors： Adamson SR,Stevenson KJ

更新日期：1981-06-09 00:00:00

abstract：:Glutaraldehyde treatment of electroplax membrane preparations of Na,K-ATPase leads to irreversible changes in the enzymic behavior of the protein, which are not due to modification of the active site. When the glutaraldehyde treatment is carried out in a medium containing K+ and without Na+, the "K+-modified enzyme" s...

journal_title：Biochemistry

authors： Chipman DM,Lev A

更新日期：1983-09-13 00:00:00

abstract：:The mechanism by which terminal guanidino nitrogen substituted analogues of amiloride inhibit Na-Ca exchange in purified cardiac sarcolemmal membrane vesicles has been investigated. These inhibitors block both Nai-dependent Ca2+ uptake and Nao-dependent Ca2+ efflux. Inhibition of Na-Ca exchange monitored in K+ is nonc...

journal_title：Biochemistry

authors： Slaughter RS,Garcia ML,Cragoe EJ Jr,Reeves JP,Kaczorowski GJ

更新日期：1988-04-05 00:00:00

abstract：:A series of cysteine-bearing hydrophobic polypeptides analogous to a light-harvesting one betapolypeptide (LH1beta) from the LH1 complex from the purple photosynthetic bacterium, Rhodobacter sphaeroides, was synthesized using an Escherichia coli expression system. The cysteine was placed in the C- or N-terminal region...

journal_title：Biochemistry

authors： Dewa T,Yamada T,Ogawa M,Sugimoto M,Mizuno T,Yoshida K,Nakao Y,Kondo M,Iida K,Yamashita K,Tanaka T,Nango M

更新日期：2005-04-05 00:00:00

abstract：:The temperature dependence of the packing (order) and fluidity (microviscosity) of rabbit small, intestinal brush border vesicle membranes and of liposomes made from their extracted lipids has been investigated by using a variety of lipid spin probes. The lipids in the brush border membrane are present essentially as ...

journal_title：Biochemistry

authors： Hauser H,Gains N,Semenza G,Spiess M

更新日期：1982-10-26 00:00:00

abstract：:To test the prediction that extensively fluorinated (fluorous) proteins should be more stable and exhibit novel self-segregating behavior, the properties of the de novo designed model 4-alpha-helix bundle protein, alpha 4F 6, in which the hydrophobic core is packed entirely with the extensively fluorinated amino acid ...

journal_title：Biochemistry

authors： Gottler LM,de la Salud-Bea R,Marsh EN

更新日期：2008-04-15 00:00:00

abstract：:The ATPase activity of the (Ca2+-Mg2+)-ATPase reconstituted into bilayers of phosphatidylcholines depends on the fatty acyl chain length of the phospholipids. It is shown that the fluorescence response to Ca2+ of the ATPase modified with fluorescein isothiocyanate is also dependent on phospholipid structure and is int...

journal_title：Biochemistry

authors： Froud RJ,East JM,Jones OT,Lee AG

更新日期：1986-11-18 00:00:00

abstract：:Initial rate kinetic studies and reduced coenzyme binding studies with bovine glutamate dehydrogenase have shown that an enzyme-NAD(P)H-glutamate abortive complex is a major participant in the overall enzyme-catalyzed reaction. The allosteric regulator ADP is shown to activate the enzyme by destabilizing this abortive...

journal_title：Biochemistry

authors： George A,Bell JE

更新日期：1980-12-23 00:00:00

abstract：:Proteogenomic identification of translated small open reading frames in humans has revealed thousands of microproteins, or polypeptides of fewer than 100 amino acids, that were previously invisible to geneticists. Hundreds of microproteins have been shown to be essential for cell growth and proliferation, and many reg...

journal_title：Biochemistry

authors： Na Z,Luo Y,Schofield JA,Smelyansky S,Khitun A,Muthukumar S,Valkov E,Simon MD,Slavoff SA

更新日期：2020-10-27 00:00:00

abstract：:An in vitro system reconstituted with mouse liver polysome translation products was used to study the nature of polypeptide species imported into mitochondria from different mouse tissues such as liver, kidney, brain, and heart, as well as from Ehrlich ascites, Novikoff hepatoma, and Morris hepatoma 3924A tumor lines....

journal_title：Biochemistry

authors： Bhat NK,Avadhani NG

更新日期：1985-12-31 00:00:00

abstract：:Administration of pregnenolone-16 alpha-carbonitrile (PCN) to adult female rats caused a 2-fold increase in total liver microsomal cytochrome P-450 along with 5-7-fold increases in four in vitro monooxygenase activities considered diagnostic for the major PCN-inducible cytochrome P-450 isozyme. However, upon administr...

journal_title：Biochemistry

authors： Graves PE,Kaminsky LS,Halpert J

更新日期：1987-06-30 00:00:00

abstract：:Pepstatin is a slow and tight-binding inhibitor of pepsin. Preincubating enzyme and inhibitor in H2O and in D2O in the absence of substrate generates an inverse solvent isotope effect of Dk = 0.69 +/- 0.06 on the apparent first-order rate constant for the decay in enzymatic activity. Proton inventory analysis of the i...

journal_title：Biochemistry

authors： Cho YK,Rebholz KL,Northrop DB

更新日期：1994-08-16 00:00:00

abstract：:For the class A β-lactamase SHV-1, the kinetic and mechanistic properties of the clinically used inhibitor sulbactam are compared with the sulbactam analog substituted in its 6β position by a CH2OH group (6β-(hydroxymethyl)penicillanic acid). The 6β substitution improves both in vitro and microbiological inhibitory pr...

journal_title：Biochemistry

authors： Che T,Rodkey EA,Bethel CR,Shanmugam S,Ding Z,Pusztai-Carey M,Nottingham M,Chai W,Buynak JD,Bonomo RA,van den Akker F,Carey PR

更新日期：2015-01-27 00:00:00

abstract：:β-Lactam antibiotics have faced obsolescence with the emergence of methicillin-resistant Staphylococcus aureus (MRSA). A complex set of events ensues upon exposure of MRSA to these antibiotics, which culminates in proteolysis of BlaI or MecI, two gene repressors, and results in the induction of resistance. We report s...

journal_title：Biochemistry

authors： Blázquez B,Llarrull LI,Luque-Ortega JR,Alfonso C,Boggess B,Mobashery S

更新日期：2014-03-18 00:00:00

abstract：:The objective of this work is to determine the molecular mechanism and regulation of short-chain acyl-CoA dehydrogenase (SCAD) from Megasphaera elsdenii. To achieve this, the gene coding for SCAD from M. elsdenii was cloned and sequenced. Site-directed mutagenesis was then used to identify an amino acid residue that i...

journal_title：Biochemistry

authors： Becker DF,Fuchs JA,Banfield DK,Funk WD,MacGillivray RT,Stankovich MT

更新日期：1993-10-12 00:00:00