Abstract:
:A series of cysteine-bearing hydrophobic polypeptides analogous to a light-harvesting one betapolypeptide (LH1beta) from the LH1 complex from the purple photosynthetic bacterium, Rhodobacter sphaeroides, was synthesized using an Escherichia coli expression system. The cysteine was placed in the C- or N-terminal regions of the polypeptide to investigate the influence of steric confinement and orientation of the polypeptides via disulfide linkages as they were self-assembled with zinc-substituted bacteriochlorophyll a ([Zn]-BChl a). The polypeptides were expressed as water-soluble fusion proteins with maltose-binding protein (MBP). The fusion proteins formed a subunit-type complex with the [Zn]-BChl a in an n-octyl-beta-d-glucopyranoside (OG) micellar solution regardless of the cross-links or the cleavage of the cysteines, judging from absorption, CD, and fluorescence spectra. Following treatment with trypsin, the polypeptides were detached from the MBP portion. Such trypsin-digested polypeptides formed a subunit-type LH complex at 25 degrees C, which also showed that the disulfide linkage was not crucial for the subunit formation. When a polypeptide having cysteine on the C-terminus was assembled at 4 degrees C, the Qy absorption band was remarkably red-shifted to approximately 836 nm, suggesting that the cleavage of the large MBP portion liberates the polypeptides to form the progressive type of complex similar to LH1-type complex. The trypsin-treated polypeptides bearing cysteines in both terminal regions, which are randomly cross-linked, did not form the LH1-type complex under oxidative conditions but did form the complex under reductive conditions. This observation suggests that the polypeptide orientation strongly influences the LH1-type complex formation. The progressive assembly from the subunit to the holo-LH1-type complex following cleavage of MBP portion in a lipid bilayer is also briefly discussed.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Dewa T,Yamada T,Ogawa M,Sugimoto M,Mizuno T,Yoshida K,Nakao Y,Kondo M,Iida K,Yamashita K,Tanaka T,Nango Mdoi
10.1021/bi0478493subject
Has Abstractpub_date
2005-04-05 00:00:00pages
5129-39issue
13eissn
0006-2960issn
1520-4995journal_volume
44pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The functional diversity of small GTP-binding proteins (G proteins) and their ability to function as molecular switches are based on their interactions with many different proteins. A wealth of structural data has revealed that their partners are often unrelated to each other in sequence and structure, but their bindi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049630u
更新日期:2004-06-08 00:00:00
abstract::Thromboxane A(2) receptor (TP receptor), a prostanoid receptor, belongs to the G protein-coupled receptor family, composed of three intracellular loops and three extracellular loops connecting seven transmembrane helices. The highly conserved extracellular domains of the prostanoid receptors were found in the second e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001867c
更新日期:2001-01-09 00:00:00
abstract::New classes of small proteins have recently been found that refold rapidly with two-state kinetics from a substantially unfolded conformation ("U") and without the accumulation of a folding intermediate. Barnase, on the other hand, is representative of a class of proteins that display multistate kinetics and refold fr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi950967t
更新日期:1996-02-27 00:00:00
abstract::A major issue for organisms living at extreme temperatures is to preserve both stability and activity of their enzymes. Cold-adapted enzymes generally have a reduced thermal stability, to counteract freezing, and show a lower enthalpy and a more negative entropy of activation compared to mesophilic and thermophilic ho...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801177k
更新日期:2008-09-23 00:00:00
abstract::The conformation of the GM3 ganglioside, Neu5Ac alpha 2-3Gal beta 1-4Glc beta 1-1 Cer, and its analogs containing the Neu5Gc or Neu4Ac5Gc residues (Gc = glycolyl, CH2OHCO) was investigated in Me2SO-d6 solution with the aid of a distance-mapping procedure based on rotating-frame NOE contacts, with hydroxyl protons bein...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00145a014
更新日期:1992-08-04 00:00:00
abstract::Aldehyde dehydrogenase 7A1 (ALDH7A1) is part of lysine catabolism and catalyzes the NAD(+)-dependent oxidation of α-aminoadipate semialdehyde to α-aminoadipate. Herein, we describe a structural study of human ALDH7A1 focused on substrate recognition. Five crystal structures and small-angle X-ray scattering data are re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00754
更新日期:2015-09-08 00:00:00
abstract::The effects of metal ion binding on the optical spectroscopic properties and temperature stability of two single tryptophan mutants of chicken skeletal TnC, F78W and F154W, have been examined. The absence of tyrosine and other tryptophan residues allowed the unambiguous assignment of the spectral signal from the intro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982048j
更新日期:1999-09-14 00:00:00
abstract::Glycophorin A is a sialoglycoprotein isolated from human erythrocyte membranes which seems to exist as stable dimeric complexes in the presence of sodium dodecyl sulfate. When analyzed by dodecyl sulfate acrylamide electrophoresis this molecule forms two PAS-stainable bands (PAS-U and PAS-2) which are reversibly inter...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00650a028
更新日期:1976-03-09 00:00:00
abstract::The glycolytic enzyme fructose-1,6-bisphosphate aldolase (FBPA) catalyzes the reversible cleavage of fructose 1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Catalysis of Schiff base forming class I FBPA relies on a number of intermediates covalently bound to the catalytic lysine. Using ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048192o
更新日期:2005-03-22 00:00:00
abstract::The relative roles of insulin and glucose in the regulation of hepatic glycogen synthase and phosphorylase were studied in hepatocytes from fed rats. Elevation of extra-cellular glucose led to a rapid decrease in phosphorylase a activity followed by a slower increase in glycogen synthase I activity. A reciprocal and c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00596a004
更新日期:1978-02-07 00:00:00
abstract::X-ray absorption spectra are reported for the multi-Cu oxidase Rhus vernicifera laccase in oxidized and fully reduced forms and for laccase from which the type 2 Cu has been depleted (T2D). The structure of the Cu K edge for both preparations shows the presence of CuII and CuI in the oxidized and reduced states, respe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00310a008
更新日期:1984-07-17 00:00:00
abstract::In our previous papers, seven structural genes (NQO1-7) of the energy-transducing NADH-quinone (Q) oxidoreductase of Paracoccus denitrificans were characterized [Xu, X., Matsuno-Yagi, A., & Yagi, T. (1991a) Biochemistry 30, 8678-8684; (1991b) Biochemistry 30, 6422-6428; (1992a) Biochemistry 31, 6925-6932; (1992b) Arch...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00054a030
更新日期:1993-01-26 00:00:00
abstract::The Gbeta and Ggamma subunit of the heterotrimeric G proteins form a functional dimer that is stable once assembled in vivo or in vitro. The requirements, mechanism, and specificity of dimer formation are still incompletely understood, but represent important biochemical processes involved in the specificity of cellul...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0504254
更新日期:2005-09-06 00:00:00
abstract::Membrane preparations from two independent ouabain-resistant HeLa cell clones, HI-B1 and HI-C1, each appear to contain two species of (Na,K)ATPase. Two-thirds of the total (Na,K)ATPase in each mutant is indistinguishable from the enzyme in preparations of wild type cells with respect to ouabain binding, ouabain inhibi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00642a600
更新日期:1977-11-15 00:00:00
abstract::Bacteriophage T7 codes for a single-stranded DNA binding protein. This protein is the product of gene 2.5 and has been found previously to stimulate specifically the activity of the phage-coded DNA polymerase. We report here that the T7 DNA binding protein also stimulates the activity of the phage-coded exonuclease. T...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00266a042
更新日期:1982-11-09 00:00:00
abstract::The technique of fluorescence recovery after photobleaching is used to perform an extensive study of the lateral diffusion of a phospholipid probe in the binary mixture dimyristoylphosphatidylcholine/cholesterol, above the melting temperature of the phospholipid. In the regions of the phase diagram where a single liqu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00144a013
更新日期:1992-07-28 00:00:00
abstract::The present study was undertaken for the purpose of evaluating the solvent perturbation technique as a method to identify enzyme catalytic residues. For establishment of expected directions and sizes of pKa perturbations for different types of acids in different classes of solvents, a study of the pKa of a series of a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00287a013
更新日期:1983-08-30 00:00:00
abstract::PKC phosphorylation regulates PITX2 DNA binding and transcriptional activity. Mutation of individual PKC sites demonstrates the functional regulation of PITX2 through phosphorylation. Immunoprecipitation of PITX2 and a PITX2 PKC mutant protein reveal specific in vivo phosphorylation by PKC in transfected cells. The tr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048362x
更新日期:2005-03-15 00:00:00
abstract::Kinetics studies of dNTP analogues having pyrophosphate-mimicking β,γ-pCXYp leaving groups with variable X and Y substitution reveal striking differences in the chemical transition-state energy for DNA polymerase β that depend on all aspects of base-pairing configurations, including whether the incoming dNTP is a puri...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500101z
更新日期:2014-03-25 00:00:00
abstract::Our previous studies demonstrated that the first 1000 amino acid residues (the βα1 domain) of human apolipoprotein (apo) B-100, termed apoB:1000, are required for the initiation of lipoprotein assembly and the formation of a monodisperse stable phospholipid (PL)-rich particle. The objectives of this study were (a) to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01174
更新日期:2017-08-08 00:00:00
abstract::The aggregation of the microtubule-associated protein, tau, into amyloid fibrils is a hallmark of neurodegenerative diseases such as the tauopathies and Alzheimer's disease. Since monomeric tau is an intrinsically disordered protein and the polymeric fibrils possess an ordered cross-β core, the aggregation process is ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500528x
更新日期:2014-10-21 00:00:00
abstract::DNA three-way junctions (3WJs) are branched structures that serve as important biological intermediates and as components in DNA nanostructures. We recently derived the global structure of a fully complementary 3WJ and found that it contained unpaired bases at the branchpoint, which is consistent with previous observa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00677
更新日期:2017-09-19 00:00:00
abstract::Trypanosoma brucei ornithine decarboxylase was reconstituted by coexpression of two polypeptides corresponding to residues 1-305 and residues 306-425 in Escherichia coli. The two peptides were coexpressed, at wild-type levels, from a single transcriptional unit that was separated by a 15-nucleotide untranslated region...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00041a021
更新日期:1995-10-17 00:00:00
abstract::Adenosine A(2B) receptors, which play a role in inflammation and cancer, are of considerable interest as novel drug targets. To gain deeper insights into ligand binding and receptor activation, we exchanged amino acids predicted to be close to the binding pocket. The alanine mutants were stably expressed in CHO cells ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3012065
更新日期:2013-01-29 00:00:00
abstract::Phenylalanine 87 of Bacillus megaterium cytochrome P450 BM3, a residue close to the heme in the substrate binding pocket, has been replaced by alanine by site-directed mutagenesis. The substitution had no effect on the rate of hydroxylation of laurate and increased the affinity for laurate of both the intact enzyme an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962826c
更新日期:1997-02-18 00:00:00
abstract::The melanocortin-4 receptor (MC4R) is involved in regulating energy homeostasis and is a potential therapeutic target for obesity and cachexia. Molecular interactions between peptide ligands and MC4R have been studied in detail. Less is known regarding the role of these interactions in the mechanism of MC4R activation...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700406k
更新日期:2007-09-18 00:00:00
abstract::It had been previously reported that aromatic amines were not substrates for the bacterial quinoprotein methylamine dehydrogenase. In this study, benzylamine-dependent activity was also not observed in the steady-state assay of this enzyme with the artificial electron acceptor phenazine ethosulfate (PES). Benzylamines...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00128a012
更新日期:1992-04-07 00:00:00
abstract::Administration of pregnenolone-16 alpha-carbonitrile (PCN) to adult female rats caused a 2-fold increase in total liver microsomal cytochrome P-450 along with 5-7-fold increases in four in vitro monooxygenase activities considered diagnostic for the major PCN-inducible cytochrome P-450 isozyme. However, upon administr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00387a022
更新日期:1987-06-30 00:00:00
abstract::Extracellular cAMP induces the rapid activation of guanylate cyclase, which adapts within 10 s to constant cAMP concentrations. A new response can be induced either by a higher cAMP concentration or by the same cAMP concentration at some time (t1/2 = 90 s) after removal of the previous stimulus. Stimulation of guanyla...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00397a047
更新日期:1987-11-17 00:00:00
abstract::Bovine hoof keratin was shown to be a substrate for cAMP-dependent protein kinase using [gamma-32P]ATP. Natural-abundance cross-polarization (CP) MAS 13C NMR was used to examine the effect of phosphorylation on keratin structure. When short contact times were used, phosphorylation was shown to increase the number of r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00458a023
更新日期:1990-02-13 00:00:00