Abstract:
:β-Lactam antibiotics have faced obsolescence with the emergence of methicillin-resistant Staphylococcus aureus (MRSA). A complex set of events ensues upon exposure of MRSA to these antibiotics, which culminates in proteolysis of BlaI or MecI, two gene repressors, and results in the induction of resistance. We report studies on the mechanism of binding of these gene repressors to the operator regions by fluorescence anisotropy. Within the range of in vivo concentrations for BlaI and MecI, these proteins interact with their regulatory elements in a reversible manner, as both a monomer and a dimer.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Blázquez B,Llarrull LI,Luque-Ortega JR,Alfonso C,Boggess B,Mobashery Sdoi
10.1021/bi500074wsubject
Has Abstractpub_date
2014-03-18 00:00:00pages
1548-50issue
10eissn
0006-2960issn
1520-4995journal_volume
53pub_type
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