Abstract:
:Porcine aortic myosin is a smooth muscle contractile protein similar to its striated muscle counterpart. Electrophoresis in sodium dodecyl sulfate indicates that the molecule consists of three classes of subunits with polypeptide chain molecular weights of 192,000, 19,000, and 15,000. At 277 nm the absorption spectrum gives an extinction coefficient for aortic myosin of 0.558 cm2/mg; the circular dichroism spectrum of the protein indicates that aortic myosin contains about 70% of its residues in the alpha-helical configuration. Amino acid analysis shows that the smooth muscle myosin has significantly more arginine and leucine and significantly less valine and isoleucine than rabbit skeletal muscle myosin. Other studies yielded these data: Vapp = 0.716 mL/g [eta] = 0.213 mL/mg, S20, w = 5.84 x 10(-13)S. Similar studies with rabbit skeletal muscle myosin indicate that Vapp = 0.711 mL/g and S20, w = 6.36 x 10(-13)S. These properties suggest that aortic myosin, like skeletal muscle myosin, behaves hydrodynamically like a rigid rod.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Frederiksen DWdoi
10.1021/bi00576a003subject
Has Abstractpub_date
1979-05-01 00:00:00pages
1651-6issue
9eissn
0006-2960issn
1520-4995journal_volume
18pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::We have used a site-specific approach to investigate the mutagenic potential of (+)- and (-)-trans-anti-benzo[a]pyrene diol epoxide (BPDE) DNA adducts. Oligodeoxyribonucleotides (5'TCCTCCTG1G2-CCTCTC), modified at the exocyclic amino groups of G1 or G2, were incorporated into a single-stranded shuttle vector and intro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9608875
更新日期:1996-12-24 00:00:00
abstract::The X-ray crystal structure of recombinant Desulfovibrio vulgaris rubrerythrin (Rbr) that was subjected to metal constitution first with zinc and then iron, yielding ZnS(4)Rbr, is reported. A [Zn(SCys)(4)] site with no iron and a diiron site with no appreciable zinc in ZnS(4)Rbr were confirmed by analysis of the anoma...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0356193
更新日期:2004-03-23 00:00:00
abstract::The pathway for utilization of pyridine nucleotide derived reducing equivalents in the cytochrome P-450 monooxygenase systems has three major branch points. The first is a partitioning between autoxidation of a ferrous, oxygenated heme adduct and input of the second reducing equivalent required for monooxygenase stoic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00094a009
更新日期:1993-11-02 00:00:00
abstract::Ultraviolet thermal denaturation studies substantiate our earlier hypothesis that substitution of a L-nucleotide residue for a D-nucleotide within a DNA duplex permits a stable structure in which all bases are paired through Watson-Crick hydrogen bonds (Damha, M. J., Giannaris, P. A., Marfey, P., & Reid, L. S. (1991) ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00191a015
更新日期:1994-06-28 00:00:00
abstract::A method is described for measuring equilibrium constants of DNA-protein interactions using gel chromatography. This technique has been used to study the sequence-specific interaction of the HinfI restriction endonuclease with DNA. HinfI has a monomeric molecular weight of 31000 and exists as a dimer in its active for...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00333a037
更新日期:1985-06-04 00:00:00
abstract::Heme propionate-7 in cytochrome c is an ionizable group located in a region of the protein that is inaccessible to bulk solvent. Electrostatic stabilization of this functional group appears to be achieved through interaction of heme propionate-7 with several amino acid residues that occur within hydrogen-bonding dista...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00071a019
更新日期:1993-05-25 00:00:00
abstract::Pancreatic and duodenal homeobox 1 (Pdx1) is a homeodomain transcription factor belonging to the ParaHox family. Pdx1 plays an essential role in pancreatic endocrine and exocrine cell development and maintenance of adult islet beta-cell function. Mutations in the human pdx1 gene are linked to an early onset form of no...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060969l
更新日期:2007-03-20 00:00:00
abstract::The respiratory chain in the cytochrome d deficient mutant Escherichia coli GR19N is a relatively simple, linear system consisting of primary dehydrogenases, ubiquinone 8, cytochrome b-556, and cytochrome o oxidase. By use of right-side-out and inside-out membrane vesicles from this strain, various oxidase activities ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00357a004
更新日期:1986-05-06 00:00:00
abstract::To identify the residues essential for interfacial binding and substrate binding of human pancreatic phospholipase A2 (hpPLA2), several ionic residues in the putative interfacial binding surface (R6E, K7E, K10E, and K116E) and substrate binding site (D53K and K56E) were mutated. Interfacial affinity of these mutants w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990600e
更新日期:1999-06-15 00:00:00
abstract::A solubilized preparation of steroid 5 alpha-reductase (EC 1.3.1.30) from rat liver has been used in studies focused toward an understanding of the kinetic mechanism associated with enzyme catalysis. From the results of analyses with product and dead-end inhibitors, a preferentially ordered binding of substrates and r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00463a025
更新日期:1990-03-20 00:00:00
abstract::4-Hydroxybenzoyl-CoA reductase (4-HBCR) is a member of the xanthine oxidase (XO) family of molybdenum cofactor containing enzymes and catalyzes the irreversible removal of a phenolic hydroxy group by reduction, yielding benzoyl-CoA and water. In this work the effects of various activity modulating compounds were chara...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800137v
更新日期:2008-04-29 00:00:00
abstract::Ceruloplasmin is unique among the multicopper oxidases in that in addition to the usual copper stoichiometry of one Type 1 copper site and a Type 2/Type 3 trinuclear copper cluster, it contains two other Type 1 sites. This assignment of copper sites, based on copper quantitation, sequence alignment, and crystallograph...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980434v
更新日期:1998-06-30 00:00:00
abstract::In an effort to better understand protein-protein photoaffinity cross-linking using aryl azides, we have tested a number of factors influencing the cross-linking of the sigma 70 subunit of Escherichia coli RNA polymerase to core RNA polymerase. These factors include the effect of the incubations necessary for the deri...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00206a012
更新日期:1994-10-11 00:00:00
abstract::Long-chain alkanols are general anesthetics which can also act as uncharged noncompetitive inhibitors of the peripheral nicotinic acetylcholine receptor (AChR) by binding to one or more specific sites on the AChR. Cembranoids are naturally occurring, uncharged noncompetitive inhibitors of peripheral and neuronal AChRs...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0112255
更新日期:2001-09-18 00:00:00
abstract::Fusion of a prokaryotic promoter to a yeast tRNA gene provides a means for uncoupling analyses of mutations affecting splicing from requirements for transcription and other processing steps. For this purpose, a phage lambda promoter was fused to the Saccharomyces cerevisiae tRNATyr(SUP3a) coding sequence. This fusion ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00240a017
更新日期:1991-07-02 00:00:00
abstract::Deuterium NMR has been used to investigate the structure and dynamic state of cytochrome c complexed with bilayers of cardiolipin. Reductive methylation was employed to prepare [N epsilon, N epsilon-C2H3]lysyl cytochrome c, and deuterium exchange provided labeling of backbone sites to give [amide-2H]cytochrome c or mo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00230a010
更新日期:1991-04-23 00:00:00
abstract::The kinetics of the interconversions of the subgel and gel phases in dipalmitoylphosphatidylcholine have been studied by using differential dilatometry, differential scanning calorimetry (DSC), and neutral buoyancy centrifugation as a function of incubation temperature and deuteriation of the solvent. As seen by other...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00388a016
更新日期:1987-07-14 00:00:00
abstract::Human uracil DNA glycosylase (hUNG) plays a central role in DNA repair and programmed mutagenesis of Ig genes, requiring it to act on sparsely or densely spaced uracil bases located in a variety of contexts, including U/A and U/G base pairs, and potentially uracils within single-stranded DNA (ssDNA). An interesting qu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi301562n
更新日期:2013-04-16 00:00:00
abstract::Aquaporin-4 (AQP4) is a water channel found at high concentrations around blood vessels in the brain and is organized into elaborate assemblies called square arrays. The natural functions of AQP4 and the square arrays remain unknown, but under pathophysiological conditions, AQP4 has been shown to influence brain edema...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi802231q
更新日期:2009-06-30 00:00:00
abstract::The three subunits I, II, and III of bovine procarboxypeptidase A separated by reversible dimethylmaleylation can reassociate to form the reconstituted complexes I + II, I + III, and I + II + III. Since the association II + III is not possible, subunit I appears to play a central role in the formation of the complex. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00630a028
更新日期:1977-05-31 00:00:00
abstract::Dynamin 1 is thought to mediate synaptic transmission through interactions with multiple endocytic accessory proteins in a phosphorylation-dependent manner. Previously, we have shown that DYRK1A, a chromosome 21-encoded kinase implicated in the mental retardation of Down syndrome, phosphorylates primarily serine 857 (...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi2017798
更新日期:2012-08-28 00:00:00
abstract::In the yeast Saccharomyces cerevisiae, CTP synthetase [EC 6.3.4.2; UTP:ammonia ligase (ADP-forming)] is the product of the URA7 gene. CTP synthetase was purified 503-fold to apparent homogeneity from cells bearing the URA7 gene on a multicopy plasmid that directed a 10-fold overproduction of the enzyme. The purificati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00201a028
更新日期:1994-09-06 00:00:00
abstract::The interaction between the calcium binding and inhibitory components of troponin is central to the regulation of muscle contraction. In this work, two-dimensional heteronuclear single-quantum coherence nuclear magnetic resonance (2D-¿1H,15N¿-HSQC NMR) spectroscopy was used to determine the stoichiometry, affinity, an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992579n
更新日期:2000-03-21 00:00:00
abstract::An S3 electron paramagnetic resonance (EPR) signal is observed in a variety of photosystem 2 (PS2) samples in which the oxygen-evolving complex (OEC) has been inhibited. These signals have been proposed to be due to an interaction, S2X+, between the manganese cluster in an oxidation state equivalent to S2 and an organ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00088a032
更新日期:1993-09-21 00:00:00
abstract::The fluorescence properties of three variants of alpha-lactalbumin (alpha-LA) containing a single tryptophan residue were investigated under native, molten globule, and unfolded conditions. These proteins have levels of secondary structure and stability similar to those of the wild type. The fluorescence signal in the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010004w
更新日期:2001-06-19 00:00:00
abstract::Tyrosine hydroxylase (TH) catalyzes the first step in the biosynthesis of catecholamines. Regulation of TH enzyme activity is controlled through the posttranslational modification of its regulatory domain. The regulatory domain of TH can be phosphorylated at four serines (8, 19, 31, and 40) by a variety of protein kin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7019468
更新日期:2008-02-12 00:00:00
abstract::The vitamin K epoxide reductase (VKORC1) enzyme is of primary importance in many physiological processes, i.e., blood coagulation, energy metabolism, and arterial calcification prevention, due to its role in the vitamin K cycle. Indeed, VKORC1 catalyzes reduction of vitamin K epoxide to quinone and then to hydroquinon...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b01084
更新日期:2020-04-07 00:00:00
abstract::We recently developed a new, rapid, and specific bioassay system that employs a fluorescent probe fabricated from our discovered CXCR4-specific ligand DV1. This new probe sensitively and selectively blocks the binding of native and synthetic ligands to CXCR4 at nanomolar levels, with a capability comparable to that se...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500500h
更新日期:2014-08-05 00:00:00
abstract::Isoaspartyl dipeptidase (IAD) is a member of the amidohydrolase superfamily and catalyzes the hydrolytic cleavage of beta-aspartyl dipeptides. Structural studies of the wild-type enzyme have demonstrated that the active site consists of a binuclear metal center positioned at the C-terminal end of a (beta/alpha)(8)-bar...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050008r
更新日期:2005-05-17 00:00:00
abstract::In this study, we prepared several shortened and full-length insulin analogues with substitutions at position B26. We compared the binding affinities of the analogues for rat adipose membranes with their ability to lower the plasma glucose level in nondiabetic Wistar rats in vivo after subcutaneous administration, and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi702086w
更新日期:2008-05-27 00:00:00