The 14-3-3 protein affects the conformation of the regulatory domain of human tyrosine hydroxylase.

abstract：:The arrangement of the mRNA on the Escherichia coli ribosome with respect to ribosomal RNA sites has been investigated by photochemical cross-linking experiments. mRNA analogues 51-54 nucleotides in length contained a Shine-Dalgarno sequence, a single codon for tRNA(Gly), and 4-thiouridine (s4U) in the 5' third of the...

journal_title：Biochemistry

authors： Bhangu R,Juzumiene D,Wollenzien P

更新日期：1994-03-15 00:00:00

abstract：:Crowding, which characterizes the interior of all living cells, has been shown to dramatically affect biochemical processes, leading to stabilization of compact morphologies, enhanced macromolecular associations, and altered reaction rates. Due to the crowding-mediated shift in binding equilibria toward association, c...

journal_title：Biochemistry

authors： Goobes R,Kahana N,Cohen O,Minsky A

更新日期：2003-03-04 00:00:00

abstract：:The first step of the kynurenine pathway for l-tryptophan (l-Trp) degradation is catalyzed by heme-dependent dioxygenases, tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase. In this work, we employed stopped-flow optical absorption spectroscopy to study the kinetic behavior of the Michaelis complex of C...

journal_title：Biochemistry

authors： Geng J,Weitz AC,Dornevil K,Hendrich MP,Liu A

更新日期：2020-08-04 00:00:00

abstract：:P1,P2-Bidentate Co(NH3)4PP was found to be a competitive inhibitor of pyrophosphatase vs. MgPP (Kis = 8.7 mM, pH 7) and, in the presence of Mg2+, an active substrate as well. P1,P2-Bidentate Cr(III) complexes of pyrophosphate, imidodiphosphate, and methylenediphosphonate were also competitive inhibitors vs. MgPP (pH 5...

journal_title：Biochemistry

authors： Knight WB,Fitts SW,Dunaway-Mariano D

更新日期：1981-07-07 00:00:00

abstract：:Transcarboxylase (TC) is a multisubunit enzyme that catalyzes the transfer of a carboxylate group from methylmalonyl-CoA (MMCoA) to pyruvate. The CO2- group is shuttled between the MMCoA and pyruvate binding sites by a biotin cofactor, covalently linked to the 1.3S subunit. Fully carboxylated 1.3S can be prepared in v...

journal_title：Biochemistry

authors： Rivera-Hainaj RE,Pusztai-Carey M,Venkat Reddy D,Choowongkomon K,Sönnichsen FD,Carey PR

更新日期：2002-02-19 00:00:00

abstract：:As part of a search for peptides that have specificity for selected protein kinases, the possibility that adenosine cyclic 3',5'-phosphate dependent protein kinase (A-kinase) recognizes the hydrogen-bonding potential of its peptide substrates was investigated. A-Kinase catalyzes the phosphorylation of five N alpha-met...

journal_title：Biochemistry

authors： Thomas NE,Bramson HN,Miller WT,Kaiser ET

更新日期：1987-07-14 00:00:00

abstract：:Phosphatidylserine (PS) in the plasma membrane of nonactivated human platelets is almost entirely located on the cytoplasmic side. Stimulation of platelets with the Ca2+ ionophore A23187 or combined action of collagen plus thrombin results in a rapid loss of the asymmetric distribution of PS. Also, treatment with the ...

journal_title：Biochemistry

authors： Bevers EM,Tilly RH,Senden JM,Comfurius P,Zwaal RF

更新日期：1989-03-21 00:00:00

abstract：:A general protocol for preparation of oligonucleotides containing intrastrand cross-links between the exocyclic amino groups of adjacent deoxyguanosines has been developed. A series of 2, 3, and 4 methylene cross-links was incorporated site-specifically into an 11-mer (5'-GGCAGGTGGTG-3', cross-linked positions are und...

journal_title：Biochemistry

authors： Kowalczyk A,Carmical JR,Zou Y,Van Houten B,Lloyd RS,Harris CM,Harris TM

更新日期：2002-03-05 00:00:00

abstract：:Inherently curved DNA segments, associated with short runs of adenines, have been identified in many gene regulatory regions, yet their physiological significance remains unknown. The observations reported in this study indicate that intrinsically bent nucleic acid fragments are characterized by substantially attenuat...

journal_title：Biochemistry

authors： Shatzky-Schwartz M,Hiller Y,Reich Z,Ghirlando R,Weinberger S,Minsky A

更新日期：1992-03-03 00:00:00

abstract：:A cDNA designated hIIB1, representing the entire coding sequence of a P450 in the IIB gene family, was isolated from a human liver lambda gt11 library by using the rat IIB1 cDNA as a probe. The hIIB1 protein, deduced from the cDNA sequence, contained 491 amino acids, had a calculated molecular weight of 56,286, and di...

journal_title：Biochemistry

authors： Yamano S,Nhamburo PT,Aoyama T,Meyer UA,Inaba T,Kalow W,Gelboin HV,McBride OW,Gonzalez FJ

更新日期：1989-09-05 00:00:00

abstract：:The major excreted protein (MEP) of transformed mouse fibroblasts has been purified, and monospecific antisera against it have been prepared. Synthesis and secretion of this protein have previously been shown to be stimulated by transformation or treatment with tumor-promoting phorbol esters, but its function is still...

journal_title：Biochemistry

authors： Gottesman MM,Cabral F

更新日期：1981-03-17 00:00:00

abstract：:Mammalian thioredoxin reductase (TR) is a pyridine nucleotide disulfide oxidoreductase that uses the rare amino acid selenocysteine (Sec) in place of the more commonly used amino acid cysteine (Cys) in the redox-active tetrapeptide Gly-Cys-Sec-Gly motif to catalyze thiol/disulfide exchange reactions. Sec can accelerat...

journal_title：Biochemistry

authors： Lothrop AP,Snider GW,Ruggles EL,Patel AS,Lees WJ,Hondal RJ

更新日期：2014-02-04 00:00:00

abstract：:We describe the development and characterization of substance P labeled at Lys3 with fluorescein ([fluorescein Lys3]SP) as a fluorescent probe for the neurokinin 1 (NK1) receptor. [fluorescein Lys3]SP is an agonist at the human NK1 receptor, with an affinity for both the high-affinity and low-affinity binding states o...

journal_title：Biochemistry

authors： Tota MR,Daniel S,Sirotina A,Mazina KE,Fong TM,Longmore J,Strader CD

更新日期：1994-11-08 00:00:00

abstract：:Lecithin retinol acyltransferase (LRAT) catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester, an essential reaction in the vertebrate visual cycle. Since all-trans-retinyl esters are the substrates for the isomerization reaction that generates 11-cis-retinoids, this esterification reaction is...

journal_title：Biochemistry

authors： Bok D,Ruiz A,Yaron O,Jahng WJ,Ray A,Xue L,Rando RR

更新日期：2003-05-27 00:00:00

abstract：:Helicobacter pylori NikR (HpNikR) is a nickel-responsive transcription factor. In addition to a role in nickel homeostasis, HpNikR is proposed to serve as a master activator-repressor for H. pylori acid adaptation by directly or indirectly regulating the expression of a battery of genes. One potential mechanism of thi...

journal_title：Biochemistry

authors： Li Y,Zamble DB

更新日期：2009-03-24 00:00:00

abstract：:The amino acid sequence of chick skin collagen alpha1-CB7, the 268 CNBr peptide from the helical portion of the alpha1 chain, has been determined by automatic and manual degradation of tryptic and chymotryptic peptides, and of the COOH-terminal fragment produced by cleavage with animal collagenase. The resulting seque...

journal_title：Biochemistry

authors： Highberger JH,Corbett C,Kang AH,Gross J

更新日期：1975-07-01 00:00:00

abstract：:To identify residues and segments in the central region of apolipoprotein A-I (apoA-I) that are important for the protein structure and stability, we studied the effects of four double charge ablations, D102A/D103A, E110A/E111A, R116V/K118A, and R160V/H162A, and two deletion mutations, Delta(61-78) and Delta(121-142),...

journal_title：Biochemistry

authors： Gorshkova IN,Liu T,Kan HY,Chroni A,Zannis VI,Atkinson D

更新日期：2006-01-31 00:00:00

abstract：:15N NMR spectroscopy was used to examine the active-site histidyl residue of alpha-lytic protease in peptide boronic acid inhibitor complexes. Two distinct types of complexes were observed: (1) Boronic acids that are analogues of substrates form complexes in which the active-site imidazole ring is protonated and both ...

journal_title：Biochemistry

authors： Bachovchin WW,Wong WY,Farr-Jones S,Shenvi AB,Kettner CA

更新日期：1988-10-04 00:00:00

abstract：:DB2277, a heterocyclic diamidine, is a successful design for mixed base pair (bp) DNA sequence recognition. The compound has a central aza-benzimidazole group that forms two H-bonds with a GC bp that has flanking AT bps. The nuclear magnetic resonance structure of the DB2277-DNA complex with an AAGATA recognition site...

journal_title：Biochemistry

authors： Harika NK,Wilson WD

更新日期：2018-08-21 00:00:00

abstract：:The precipitation of a monoclonal IgG2 crystalline cryoglobulin (WEB) is shown to be highly dependent on temperature and concentration. Below a critical concentration of 0.6 mg/mL there is no cryoprecipitation. The kinetics of the aggregation exhibits a concentration-dependent lag time. This evidence suggests that a n...

journal_title：Biochemistry

authors： Scoville CD,Abraham GN,Turner DH

更新日期：1979-06-12 00:00:00

abstract：:As a prerequisite to mammalian fertilization, the sperm acrosomal vesicle fuses with the plasma membrane and the acrosome contents are exocytosed. Induction occurs through engagement of the sperm receptors by multiple sugar residues. Multivalent polymers displaying mannose, fucose, or GlcNAc are effective synthetic in...

journal_title：Biochemistry

authors： Huang H,Rodolis MT,Bhatia SR,Sampson NS

更新日期：2017-06-06 00:00:00

abstract：:Cytomegaloviruses (CMVs) are ubiquitous species-specific viruses that establish acute, persistent, and latent infections. Both human and mouse CMVs encode proteins that inhibit the activation of natural killer (NK) cells by downregulating cellular ligands for the NK cell activating receptor, NKG2D. The MCMV glycoprote...

journal_title：Biochemistry

authors： Zhi L,Mans J,Paskow MJ,Brown PH,Schuck P,Jonjić S,Natarajan K,Margulies DH

更新日期：2010-03-23 00:00:00

abstract：:The spontaneous hydrolyses of lactic acid O-phenyl phosphate (I) and, to a lesser extent, 3-hydroxybutyric acid O-phenyl phosphate (II) have been investigated and compared with similar intramolecular and bimolecular reactions. Compared to bimolecular nucleophilic reactions, the reactivity of II is similar to other sys...

journal_title：Biochemistry

authors： Steffens JJ,Siewers IJ,Benkovic SJ

更新日期：1975-06-03 00:00:00

abstract：:Cuprizone intoxication is a common animal model used to test myelin regenerative therapies for the treatment of diseases such as multiple sclerosis. Mice fed this copper chelator develop reversible, region-specific oligodendrocyte loss and demyelination. While the cellular changes influencing the demyelinating process...

journal_title：Biochemistry

authors： Taraboletti A,Walker T,Avila R,Huang H,Caporoso J,Manandhar E,Leeper TC,Modarelli DA,Medicetty S,Shriver LP

更新日期：2017-03-14 00:00:00

abstract：:Small molecule allostery modifies protein function but is not easily discovered. We introduce mass spectrometry integrated with equilibrium dialysis for the discovery of allostery systematically (MIDAS), a method for identifying physiologically relevant, low-affinity metabolite-protein interactions using unmodified pr...

journal_title：Biochemistry

authors： Orsak T,Smith TL,Eckert D,Lindsley JE,Borges CR,Rutter J

更新日期：2012-01-10 00:00:00

abstract：:The stability of three forms of glucoamylase from Aspergillus niger has been investigated by differential scanning and isothermal titration calorimetry: Glucoamylase 1 (GA1), which consists of a catalytic domain and a starch-binding domain (SBD) connected by a heavily O-glycosylated linker region; glucoamylase 2 (GA2)...

journal_title：Biochemistry

authors： Christensen T,Svensson B,Sigurskjold BW

更新日期：1999-05-11 00:00:00

abstract：:The disulfide bond location of a homodimeric peptide, prothoracicotropic hormone (PTTH) of the silkworm, Bombyx mori, was determined by a combination of partial reduction and sequence analysis of peptide fragments generated through a partial reduction of PTTH followed by alkylation and enzyme digestion. The partial re...

journal_title：Biochemistry

authors： Ishibashi J,Kataoka H,Isogai A,Kawakami A,Saegusa H,Yagi Y,Mizoguchi A,Ishizaki H,Suzuki A

更新日期：1994-05-17 00:00:00

abstract：:We studied the urea-induced unfolding transition of staphylococcal nuclease (SNase) and its five proline mutants (P47A, P47T, P117G, P47T/P117G, and P47A/P117G) [corrected] by peptide and aromatic circular dichroism and aromatic absorption spectroscopy at equilibrium and the refolding-unfolding kinetics of the protein...

journal_title：Biochemistry

authors： Ikura T,Tsurupa GP,Kuwajima K

更新日期：1997-05-27 00:00:00

abstract：:Thermodynamic data for RNA 1 x 2 nucleotide internal loops are lacking. Thermodynamic data that are available for 1 x 2 loops, however, are for loops that rarely occur in nature. In order to identify the most frequently occurring 1 x 2 nucleotide internal loops, a database of 955 RNA secondary structures was compiled ...

journal_title：Biochemistry

authors： Badhwar J,Karri S,Cass CK,Wunderlich EL,Znosko BM

更新日期：2007-12-18 00:00:00

abstract：:The E2 protein of papillomavirus is the key regulator of viral transcription and replication. Dimerization, which takes place via its conserved C-terminal DNA-binding domain (DBD), is critical for these functions. The presence and conservation of two histidines (H290 and H320) at or near the dimer interface suggests t...

journal_title：Biochemistry

authors： Bose K,Yoder NC,Kumar K,Baleja JD

更新日期：2007-02-06 00:00:00