当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Formation of a bis(histidyl) heme iron complex in manganese peroxidase at high pH and restoration of the native enzyme structure by calcium.

Formation of a bis(histidyl) heme iron complex in manganese peroxidase at high pH and restoration of the native enzyme structure by calcium.

Abstract：

:Manganese peroxidase (MnP) from Phanerochaete chrysosporium undergoes a pH-dependent conformational change evidenced by changes in the electronic absorption spectrum. This high- to low-spin alkaline transition occurs at approximately 2 pH units lower in an F190I mutant MnP when compared to the wild-type enzyme. Herein, we provide evidence that these spectral changes are attributable to the formation of a bis(histidyl) heme iron complex in both proteins at high pH. The resonance Raman (RR) spectra of both ferric proteins at high pH are similar, indicating similar heme environments in both proteins, and resemble that of ferric cytochrome b(558), a protein that contains a bis-His iron complex. Upon reduction with dithionite at high pH, the visible spectra of both the wild-type and F190I MnP exhibit absorption maxima at 429, 529, and 558 nm, resembling the absorption spectrum of ferrous cytochrome b(558). RR spectra of the reduced wild-type and F190I mutant proteins at high pH are also similar to the RR spectrum of ferrous cytochrome b(558), further suggesting that the alkaline low-spin species is a bis(histidyl) heme derivative. No shift in the low-frequency RR bands was observed in 75% (18)O-labeled water, indicating that the low-spin species is most likely not a hydroxo-heme derivative. Electronic and RR spectra also indicate that addition of Ca(2+) to either the ferric or ferrous enzymes at high pH completely restores the high-spin pentacoordinate species. Other divalent metals, such as Mn(2+), Mg(2+), Zn(2+), or Cd(2+), do not restore the enzyme under the conditions studied.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Youngs HL,Moënne-Loccoz P,Loehr TM,Gold MH

doi

10.1021/bi000679j

subject

Has Abstract

pub_date

2000-08-15 00:00:00

pages

9994-10000

issue

32

eissn

0006-2960

issn

1520-4995

pii

bi000679j

journal_volume

39

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Cross-linking of endothelin 1 and endothelin 3 to rat brain membranes: identification of the putative receptor(s).

    abstract：:Affinity-labeling experiments with 125I-endothelin derivatives using bifunctional cross-linking reagents were carried out in an attempt to identify the polypeptide component(s) of the endothelin/sarafotoxin receptors in rat brain tissues. In rat cerebellum, cortex, and caudate putamen, endothelin 1 specifically labele...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00479a012

    authors： Ambar I,Kloog Y,Sokolovsky M

    更新日期：1990-07-10 00:00:00

  • Exonucleolytic proofreading during replication of repetitive DNA.

    abstract：:We are attempting to understand the processes required to accurately replicate the repetitive DNA sequences whose instability is associated with several human diseases. Here we test the hypothesis that the contribution of exonucleolytic proofreading to frameshift fidelity during replication of repetitive DNA sequences...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952178h

    authors： Kroutil LC,Register K,Bebenek K,Kunkel TA

    更新日期：1996-01-23 00:00:00

  • Kinetic, spectroscopic, and X-ray crystallographic characterization of the functional E151H aminopeptidase from Aeromonas proteolytica.

    abstract：:Glutamate151 (E151) has been shown to be catalytically essential for the aminopeptidase from Vibrio proteolyticus (AAP). E151 acts as the general acid/base during the catalytic mechanism of peptide hydrolysis. However, a glutamate residue is not the only residue capable of functioning as a general acid/base during cat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0505823

    authors： Bzymek KP,Moulin A,Swierczek SI,Ringe D,Petsko GA,Bennett B,Holz RC

    更新日期：2005-09-13 00:00:00

  • Spatial proximity of the glycine-rich loop and the SH2 thiol in myosin subfragment 1.

    abstract：:Subfragment 1 (S1) prepared from rabbit skeletal muscle myosin was digested with trypsin to cleave the 95K heavy chain into three pieces, i.e., the 23K, 50K, and 20K fragments. The trypsin-treated S1 was then cross-linked with p-nitrophenyl iodoacetate. The cross-linker bridged one of the reactive thiols (SH2) in the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00408a045

    authors： Sutoh K,Hiratsuka T

    更新日期：1988-04-19 00:00:00

  • Binding of protein synthesis initiation factor 4E to oligoribonucleotides: effects of cap accessibility and secondary structure.

    abstract：:The binding of rabbit globin mRNA to the 25-kDa cap binding protein eIF-4E from human erythrocytes was found to be 5.3-fold stronger than the binding of the cap analogue m7GpppG to eIF-4E [Gross et al. (1990) Biochemistry 29, 5008-5012]. In order to investigate whether this effect is due to the longer sequence of nucl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00120a020

    authors： Carberry SE,Friedland DE,Rhoads RE,Goss DJ

    更新日期：1992-02-11 00:00:00

  • Solution structures of spinach acyl carrier protein with decanoate and stearate.

    abstract：:Acyl carrier protein (ACP) is a cofactor in a variety of biosynthetic pathways, including fatty acid metabolism. Thus, it is of interest to determine structures of physiologically relevant ACP-fatty acid complexes. We report here the NMR solution structures of spinach ACP with decanoate (10:0-ACP) and stearate (18:0-A...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052062d

    authors： Zornetzer GA,Fox BG,Markley JL

    更新日期：2006-04-25 00:00:00

  • Stiffness of Cargo-Motor Linkage Tunes Myosin VI Motility and Response to Load.

    abstract：:We examine the effect of cargo-motor linkage stiffness on the mechanobiological properties of the molecular motor myosin VI. We use the programmability of DNA nanostructures to modulate cargo-motor linkage stiffness and combine it with high-precision optical trapping measurements to measure the effect of linkage stiff...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00422

    authors： Shrivastava R,Rai A,Salapaka M,Sivaramakrishnan S

    更新日期：2019-11-26 00:00:00

  • Characterization of human calcitonin fibrillation in aqueous urea solution by 1H NMR spectroscopy.

    abstract：:The inhibitory effects of urea on the normally rapid fibrillation of human calcitonin (hCT) were investigated by 1H NMR. From subtle differences in the chemical shift of hCT in the presence and absence of urea, the occurrence of weak interactions between urea and hCT was confirmed. The chemical shifts on the NH and C ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961013l

    authors： Kanaori K,Nosaka AY

    更新日期：1996-10-01 00:00:00

  • Impact of actin glutathionylation on the actomyosin-S1 ATPase.

    abstract：:Glutathionylation of intracellular proteins is an established physiological regulator of protein function. In multiple models, including ischemia-reperfusion of the heart, increased oxidative stress results in the glutathionylation of sarcomeric actin. We hypothesized that actin glutathionylation may play a role in th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900669m

    authors： Pizarro GO,Ogut O

    更新日期：2009-08-11 00:00:00

  • Identification of BECN1 and ATG14 Coiled-Coil Interface Residues That Are Important for Starvation-Induced Autophagy.

    abstract：:Autophagy, an essential eukaryotic homeostasis pathway, allows the sequestration of unwanted, damaged, or harmful cytoplasmic components in vesicles called autophagosomes, permitting subsequent lysosomal degradation and nutrient recycling. Autophagosome nucleation is mediated by class III phosphatidylinositol-3-kinase...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00246

    authors： Mei Y,Su M,Sanishvili R,Chakravarthy S,Colbert CL,Sinha SC

    更新日期：2016-08-02 00:00:00

  • Affinity cleavage at the metal-binding site of phosphoenolpyruvate carboxykinase.

    abstract：:Chicken liver phosphoenolpyruvate carboxykinase (PEPCK) was rapidly inactivated by micromolar concentrations of ferrous sulfate in the presence of ascorbate at pH 7.4. Omitting ascorbate or replacing the Fe2+ with Mn2+ or Mg2+ gives no inactivation. Mn2+, Mg2+, or Co2+ at 100-fold molar excess over Fe2+ offered comple...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi970574p

    authors： Hlavaty JJ,Nowak T

    更新日期：1997-12-09 00:00:00

  • Mechanism of cysteine desulfurase Slr0387 from Synechocystis sp. PCC 6803: kinetic analysis of cleavage of the persulfide intermediate by chemical reductants.

    abstract：:Cysteine desulfurases (CDs) are pyridoxal-5'-phosphate (PLP)-dependent enzymes that cleave sulfur from cysteine via an enzyme cysteinyl persulfide intermediate. In vitro studies of these enzymes have generally employed dithiothreitol as a cosubstrate to reductively cleave the persulfide intermediate, and it has been s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049143e

    authors： Behshad E,Parkin SE,Bollinger JM Jr

    更新日期：2004-09-28 00:00:00

  • Interaction of the antitumor compound cryptophycin-52 with tubulin.

    abstract：:Cryptophycin-52 (LY355703) is currently undergoing clinical evaluation for cancer chemotherapy. It is a potent suppressor of microtubule dynamics in vitro, and low picomolar concentrations appear to inhibit cancer cell proliferation at mitosis by stabilizing spindle microtubules. In the present study, using [(3)H]cryp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0010827

    authors： Panda D,Ananthnarayan V,Larson G,Shih C,Jordan MA,Wilson L

    更新日期：2000-11-21 00:00:00

  • Light-induced conformational changes in photosynthetic reaction centers: impact of detergents and lipids on the electronic structure of the primary electron donor.

    abstract：:Light-induced hypsochromic shifts of the Q(y) absorption band of the bacteriochlorophyll dimer (P) from 865 to 850 nm were identified using continuous illumination of dark-adapted reaction centers (RCs) from Rhodobacter capsulatus when dispersed in the most commonly used detergent, the zwitterionic lauryl N-dimethylam...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200595z

    authors： Deshmukh SS,Akhavein H,Williams JC,Allen JP,Kalman L

    更新日期：2011-06-14 00:00:00

  • Human P-glycoprotein exhibits reduced affinity for substrates during a catalytic transition state.

    abstract：:Human P-glycoprotein (Pgp), a plasma membrane protein that confers multidrug resistance, functions as an ATP-dependent drug efflux pump. Pgp contains two ATP binding/utilization sites and exhibits ATPase activity that is stimulated in the presence of substrates and modulating agents. The mechanism of coupling of ATP h...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi973045u

    authors： Ramachandra M,Ambudkar SV,Chen D,Hrycyna CA,Dey S,Gottesman MM,Pastan I

    更新日期：1998-04-07 00:00:00

  • Manganese (II) and substrate interaction with unadenylylated glutamine synthetase (Escherichia coli w). II. Electron paramagnetic resonance and nuclear magnetic resonance studies of enzyme-bound manganese(II) with substrates and a potential transition-sta

    abstract：:The enhancement of the longitudinal proton relaxation rate of solvent water protons which occurs when Mn(II) is bound to the "tight" metal ion site of unadenylylated glutamine synthetase (GS) was used to determine the binding constant of L-methionine (SR)-sulfoximine to GS-Mn(II) complexes. The binary enhancement for ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00648a014

    authors： Villafranca JJ,Ash DE,Wedler FC

    更新日期：1976-02-10 00:00:00

  • Isolation and characterization of a new endo-beta-galactosidase from Diplococcus pneumoniae.

    abstract：:An endo-beta-galactosidase, which hydrolyzes the internal beta-galactosidic linkages of R----GlcNAc (or GalNAc) beta 1----3Gal beta 1----4GlcNAc (or Glc), was isolated from the culture supernatant of Diplococcus pneumoniae. The enzyme, named endo-beta-galactosidase DII, hydrolyzed linear N-acetyllactosamine repeating ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00330a008

    authors： Fukuda MN

    更新日期：1985-04-23 00:00:00

  • Quantitative analysis of binding of single-stranded DNA by Escherichia coli DnaB helicase and the DnaB x DnaC complex.

    abstract：:DnaB helicase is responsible for unwinding duplex DNA during chromosomal DNA replication and is an essential component of the DNA replication apparatus in Escherichia coli. We have analyzed the mechanism of binding of single-stranded DNA (ssDNA) by the DnaB x DnaC complex and DnaB helicase. Binding of ssDNA to DnaB he...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060118d

    authors： Biswas SB,Biswas-Fiss EE

    更新日期：2006-09-26 00:00:00

  • G-1:C73 recognition by an arginine cluster in the active site of Escherichia coli histidyl-tRNA synthetase.

    abstract：:Aminoacylation of a transfer RNA (tRNA) by its cognate aminoacyl-tRNA synthetase relies upon the recognition of specific nucleotides as well as conformational features within the tRNA by the synthetase. In Escherichia coli, the aminoacylation of tRNA(His) by histidyl-tRNA synthetase (HisRS) is highly dependent upon th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035708f

    authors： Connolly SA,Rosen AE,Musier-Forsyth K,Francklyn CS

    更新日期：2004-02-03 00:00:00

  • Organization of the human protein S genes.

    abstract：:Human genomic clones that span the entire protein S expressed gene (PS alpha) and the 3' two-thirds of the protein S pseudogene (PS beta) have been isolated and characterized. The PS alpha gene is greater than 80 kilobases in length and contains 14 introns and 15 exons, as well as 6 repetitive "Alu" sequences. Exons I...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00486a010

    authors： Schmidel DK,Tatro AV,Phelps LG,Tomczak JA,Long GL

    更新日期：1990-08-28 00:00:00

  • Electronic structure of the metal center in the Cd(2+), Zn(2+), and Cu(2+) substituted forms of KDO8P synthase: implications for catalysis.

    abstract：:Aquifex aeolicus 3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS) is active with a variety of different divalent metal ions bound in the active site. The Cd(2+), Zn(2+), and Cu(2+) substituted enzymes display similar values of k(cat) and similar dependence of K(m)(PEP) and K(m)(A5P) on both substrate and pro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801955h

    authors： Kona F,Tao P,Martin P,Xu X,Gatti DL

    更新日期：2009-04-28 00:00:00

  • Structural Characterization of the Hydratase-Aldolases, NahE and PhdJ: Implications for the Specificity, Catalysis, and N-Acetylneuraminate Lyase Subgroup of the Aldolase Superfamily.

    abstract：:NahE and PhdJ are bifunctional hydratase-aldolases in bacterial catabolic pathways for naphthalene and phenanthrene, respectively. Bacterial species with these pathways can use polycyclic aromatic hydrocarbons (PAHs) as sole sources of carbon and energy. Because of the harmful properties of PAHs and their widespread d...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00532

    authors： LeVieux JA,Medellin B,Johnson WH Jr,Erwin K,Li W,Johnson IA,Zhang YJ,Whitman CP

    更新日期：2018-06-26 00:00:00

  • Combining thermostable mutations increases the stability of lambda repressor.

    abstract：:We have combined three mutations previously shown to stabilize lambda repressor against thermal denaturation. Two of these mutations are in helix 3, where Gly-46 and Gly-48 have been replaced by alanines [Hecht, M. H., et al. (1986) Proteins: Struct., Funct., Genet. 1, 43-46]. The other mutation, which replaces Tyr-88...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00419a059

    authors： Stearman RS,Frankel AD,Freire E,Liu BS,Pabo CO

    更新日期：1988-09-20 00:00:00

  • L-cysteine desulfidase: an [4Fe-4S] enzyme isolated from Methanocaldococcus jannaschii that catalyzes the breakdown of L-cysteine into pyruvate, ammonia, and sulfide.

    abstract：:A [4Fe-4S] enzyme that decomposes L-cysteine to hydrogen sulfide, ammonia, and pyruvate has been isolated and characterized from Methanocaldococcus jannaschii. The sequence of the isolated enzyme demonstrated that the protein was the product of the M. jannaschii MJ1025 gene. The protein product of this gene was recomb...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0484769

    authors： Tchong SI,Xu H,White RH

    更新日期：2005-02-08 00:00:00

  • Structure and spectroscopy of the periplasmic cytochrome c nitrite reductase from Escherichia coli.

    abstract：:The crystal structure and spectroscopic properties of the periplasmic penta-heme cytochrome c nitrite reductase (NrfA) of Escherichia coli are presented. The structure is the first for a member of the NrfA subgroup that utilize a soluble penta-heme cytochrome, NrfB, as a redox partner. Comparison to the structures of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015765d

    authors： Bamford VA,Angove HC,Seward HE,Thomson AJ,Cole JA,Butt JN,Hemmings AM,Richardson DJ

    更新日期：2002-03-05 00:00:00

  • Phenylalanine hydroxylase: absolute configuration and source of oxygen of the 4a-hydroxytetrahydropterin species.

    abstract：:The formation of tyrosine from phenylalanine catalyzed by rat liver phenylalanine hydroxylase is coupled to the generation of a 4a-hydroxy adduct from the requisite tetrahydropterin cofactor. As indicated by its circular dichroism (CD) spectrum, the optical activity of the adduct generated from racemic 6-methyltetrahy...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00333a022

    authors： Dix TA,Bollag GE,Domanico PL,Benkovic SJ

    更新日期：1985-06-04 00:00:00

  • Orientation of cytochromes P450 in the endoplasmic reticulum.

    abstract：:The orientation of eukaryotic cytochromes P450, with respect to the membrane of the endoplasmic reticulum, has been investigated. There is now good evidence that the tertiary structure of these proteins is essentially the same as that of the soluble bacterial isoenzyme cytochrome P450CI, with the exception of an exten...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00215a011

    authors： Edwards RJ,Murray BP,Singleton AM,Boobis AR

    更新日期：1991-01-08 00:00:00

  • Characterization of cyclo-acetoacetyl-L-tryptophan dimethylallyltransferase in cyclopiazonic acid biosynthesis: substrate promiscuity and site directed mutagenesis studies.

    abstract：:The fungal neurotoxin alpha-cyclopiazonic acid (CPA), a nanomolar inhibitor of Ca(2+)-ATPase with a unique pentacyclic indole tetramic acid scaffold, is assembled by a three enzyme pathway CpaS, CpaD, and CpaO in Aspergillus sp. We recently characterized the first pathway-specific enzyme CpaS, a hybrid two module poly...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi901597j

    authors： Liu X,Walsh CT

    更新日期：2009-11-24 00:00:00

  • Characterization of ribonucleic acid polymerase-T7 promoter binary complexes.

    abstract：:Several experimental approaches have been employed to independently determine rates of dissociation for Escherichia coli RNA polymerase from the T7 bacteriophage A1, A2, A3, and D promoters. Heparin challenge measurements employing abortive initiation turnover rates as an index of promoter occupancy were carried out o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00552a023

    authors： Cech CL,McClure WR

    更新日期：1980-05-27 00:00:00

  • Cysteine auxotrophy of human leukemic lymphoblasts is associated with decreased amounts of intracellular cystathionase protein.

    abstract：:A series of human lymphoblastoid cell lines derived from nonleukemic donors are known to be cysteine prototrophs (cys+), while several lymphoblastoid lines derived from leukemic donors are cysteine auxotrophs (cys-). We have tested representative cell lines of each type for their content of cystathionase enzyme activi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00508a041

    authors： Glode LM,Kriegler MP,Livingston DM

    更新日期：1981-03-03 00:00:00