Abstract：
:An endo-beta-galactosidase, which hydrolyzes the internal beta-galactosidic linkages of R----GlcNAc (or GalNAc) beta 1----3Gal beta 1----4GlcNAc (or Glc), was isolated from the culture supernatant of Diplococcus pneumoniae. The enzyme, named endo-beta-galactosidase DII, hydrolyzed linear N-acetyllactosamine repeating structures in glycolipids and glycopeptides to release oligosaccharides. The specificity of endo-beta-galactosidase DII is the same as that of Escherichia freundii endo-beta-galactosidase as far as described above, but the following differences between these two enzymes were found: Branched lactosaminyl glycolipids and H-antigenic glycolipids were resistant to endo-beta-galactosidase DII, even when linear structure was present at the inner part. Throughout the enzymic hydrolysis, endo-beta-galactosidase DII released mostly small oligosaccharides (tetra-, tri-, and disaccharides) from substrates, suggesting that the enzyme split off the oligosaccharides stepwise from the nonreducing terminal. Lactosaminoglycans were partially hydrolyzed by endo-beta-galactosidase DII to produce small oligosaccharides as the major product and residual glycopeptides. The residual glycopeptides were readily hydrolyzed by E. freundii endo-beta-galactosidase to produce various sizes of oligosaccharides. Keratan sulfate was not degraded by endo-beta-galactosidase DII. These properties of endo-beta-galactosidase DII characterize it as a new endo-beta-galactosidase with a unique specificity.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Fukuda MNdoi
10.1021/bi00330a008subject
Has Abstractpub_date
1985-04-23 00:00:00pages
2154-63issue
9eissn
0006-2960issn
1520-4995journal_volume
24pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Two bands of 5.8S rRNA were observed when the total RNA isolated from rat or mouse tissue was separated by electrophoresis on high-resolution polyacrylamide gels under denaturing conditions. The minor form, with a lower mobility, represented 15-35% of the total 5.8S rRNA, depending on the source of the tissue. Sequenc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00311a011
更新日期：1984-07-31 00:00:00
abstract：:Positively charged cyclic hexapeptide Phe-Arg-Cys-Arg-Cys-Phe-CONH2 (FRCRCFa) represents a group of conformationally constrained peptides that block the cardiac sarcolemma Na(+)-Ca2+ exchanger [Khananshvili et al. (1995b) J. Biol. Chem. 270, 16182-16188]. Here, we study the kinetic mechanisms of FRCRCFa-induced inhibi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961099i
更新日期：1996-12-10 00:00:00
abstract：:While the urea-mediated unfolding pathway of the Escherichia coli aspartate aminotransferase (eAATase) homodimer proceeds through a reversible three-state process with a partially folded dimeric intermediate, D D* 2U (E. Deu and J. F. Kirsch, accompanying paper), that of a cofactor-stabilized form differs. Pyridoxal p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi602632d
更新日期：2007-05-15 00:00:00
abstract：:The ATPase activity of the (Ca2+-Mg2+)-ATPase reconstituted into bilayers of phosphatidylcholines depends on the fatty acyl chain length of the phospholipids. It is shown that the fluorescence response to Ca2+ of the ATPase modified with fluorescein isothiocyanate is also dependent on phospholipid structure and is int...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00371a043
更新日期：1986-11-18 00:00:00
abstract：:The structural basis for the improvement in catalytic efficiency of the mutant E165D chicken triosephosphate isomerase by the secondary mutation, S96P, has been analyzed using a combination of X-ray crystallography and Fourier transform infrared spectroscopy. All X-ray structures were of the complex of phosphoglycoloh...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00041a041
更新日期：1995-10-17 00:00:00
abstract：:N,N'-dicyclohexylcarbodiimide (DCCD) has been reported to inhibit proton translocation by cytochrome bc(1) and b(6)f complexes without significantly altering the rate of electron transport, a process referred to as decoupling. To understand the possible role of DCCD in inhibiting the protonogenic reactions of cytochro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001482u
更新日期：2000-12-26 00:00:00
abstract：:Triglyceride analogues were synthesized in which one of the primary acyl ester functions has been replaced by an alkyl group and the secondary acyl ester bond has been replaced by an acyl amino bond. The chain length at either position was varied, and both (R)- and (S)-enantiomers of each compound were synthesized. Th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00019a020
更新日期：1995-05-16 00:00:00
abstract：:Long-range intraprotein interactions give rise to many important protein behaviors. Understanding how energy is transduced through protein structures to either transmit a signal or elicit conformational changes is therefore a current challenge in structural biology. In an effort to understand such linkages, multiple V...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060652l
更新日期：2006-06-27 00:00:00
abstract：:Fourier transform infrared (FTIR) spectroscopy and a library of FTIR marker bands have been used to examine the structure and relative flexibilities conferred by different flanking sequences on the EcoRI binding site. This approach allowed us to examine unique peaks and subtle changes in the spectra of d(AAAGAATTCTTT)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8015235
更新日期：2009-02-17 00:00:00
abstract：:Calmodulin (CaM) is a small acidic protein essential to calcium-mediated signal transduction. Conformational change driven by calcium binding controls its selective activation of myriad target proteins. In most well characterized cases, both homologous domains of CaM interact with a target protein. However, physiologi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0023091
更新日期：2001-01-30 00:00:00
abstract：:The binding of ethidium bromide to the self-complementary deoxytetranucleotides containing guanine and cytosine bases has been studied by circular dichroism, visible absorption, and fluorescence spectroscopies. The circular dichroism spectrum of each of the four deoxytetranucleotides was measured and compared to the s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00616a002
更新日期：1978-11-14 00:00:00
abstract：:Arachidonic acid metabolism by lipoxygenases and cytochrome P450 monooxygenases produces regioisomeric hydroperoxyeicosatetraenoic acids (HPETEs), hydroxyeicosatetraenoic acids (HETEs), epoxyeicosatrienoic acids (EETs), and dihydroxyeicosatrienoic acids (DHETs), which serve as components of cell signaling cascades. In...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001602y
更新日期：2001-01-30 00:00:00
abstract：:Triple helix-forming oligonucleotides (TFOs) bind with high affinity and specificity to homopurine-homopyrimidine sequences in DNA and have been shown to inhibit transcription of target genes in various experimental systems. In the present study, we evaluated the ability of 3'-amino-modified phosphodiester TFOs direct...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992185w
更新日期：2000-05-02 00:00:00
abstract：:Toc34 is a transmembrane protein located in the outer envelope membrane of chloroplasts and involved in transit peptide recognition. The cytosolic region of Toc34 reveals 34% alpha-helical and 26% beta-strand structure and is stabilized by intramolecular electrostatic interaction. Toc34 binds both chloroplast preprote...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011361+
更新日期：2002-02-12 00:00:00
abstract：:The cytoplasmic disassembly of Ran.GTP.importin and Ran.GTP.exportin. cargo complexes is an essential step in the corresponding nuclear import and export cycles. It has previously been shown that such disassembly can be mediated by RanBP1 in the presence of RanGAP. The nuclear pore complex protein RanBP2 (Nup358) cont...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001010f
更新日期：2000-09-26 00:00:00
abstract：:The HIV-1 virulence factor Nef interacts with the macrophage Src-family kinase Hck, resulting in constitutive kinase activation that contributes to viral replication and immune escape. Previous chemical library screens identified the diphenylfuranopyrimdine kinase inhibitor DFP-4AB, which selectively inhibits Nef-depe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00875
更新日期：2015-10-20 00:00:00
abstract：:Pyrophosphate-dependent 6-phosphofructo-1-kinase (PPi-PFK) from Propionibacterium freudenreichii was inactivated by low concentrations of the lysine-specific reagent pyridoxal phosphate (PLP) after sodium borohydride reduction. The substrates fructose 6-phosphate and fructose 1,6-bisphosphate protected against inactiv...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00135a011
更新日期：1992-05-26 00:00:00
abstract：:Histone redistributions between endogenous DNA in calf thymus chromatin and exogenous DNA from Clostridium perfringens (69% A + T) or from Micrococcus luteus (30% A + T) induced by 0.6 M NaCl or by 2% formaldehyde were studied by thermal denaturation. The observed redistribution occurred on histone Hl when the exogeno...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00666a002
更新日期：1976-10-19 00:00:00
abstract：:The modulatory action of Ca2+-calmodulin on multiple targets is inhibited by trifluoperazine, which competes with target proteins for calmodulin binding. The structure of calmodulin crystallized with two trifluoperazine molecules is determined by X-ray crystallography at 2.74 A resolution. The X-ray data together with...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980795a
更新日期：1998-11-03 00:00:00
abstract：:High hydrostatic pressure has been shown to cause reversible dissociation of the isolated apo beta 2 dimer of tryptophan synthase from Escherichia coli into enzymatically inactive monomers [Seifert, T., Bartholmes, P., & Jaenicke, R. (1982) Biophys. Chem. 15, 1-8]. Addition of the coenzyme pyridoxal 5'-phosphate affec...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00323a016
更新日期：1985-01-15 00:00:00
abstract：:Aminoacylation of a transfer RNA (tRNA) by its cognate aminoacyl-tRNA synthetase relies upon the recognition of specific nucleotides as well as conformational features within the tRNA by the synthetase. In Escherichia coli, the aminoacylation of tRNA(His) by histidyl-tRNA synthetase (HisRS) is highly dependent upon th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035708f
更新日期：2004-02-03 00:00:00
abstract：:Plasma membrane and vesicular transporters for the biogenic amines, dopamine, norepinephrine, and serotonin, represent a group of proteins that play a crucial role in the regulation of neurotransmission. Clinically, mono amine transporters are the primary targets for the actions of many therapeutic agents used to trea...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi200405c
更新日期：2011-08-30 00:00:00
abstract：:The vanadate cofactor in vanadium chloroperoxidase has been studied using UV-VIS absorption spectroscopy. A band is present in the near-UV that is red-shifted as compared to free vanadate and shifts in both position and intensity upon change in pH. Mutation of vanadate binding residues has a clear effect on the spectr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9921790
更新日期：2000-02-08 00:00:00
abstract：:We determined the magnitude of the bend induced in DNA by an adenine-thymine tract by measuring the rate of cyclization of DNA oligonucleotides containing phased A tracts. A series of linear multimers with 2-bp single-stranded ends, in which the (A.T)6 tracts are separated by CG2-3C sequences and are positioned 10 and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00469a027
更新日期：1990-05-01 00:00:00
abstract：:The bacterial phosphotriesterase (PTE) from Pseudomonas diminuta catalyzes the hydrolysis of organophosphate esters at rates close to the diffusion limit. X-ray diffraction studies have shown that a binuclear metal center is positioned in the active site of PTE and that this complex is responsible for the activation o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800971v
更新日期：2008-09-09 00:00:00
abstract：:Incorporation of nonproteinogenic amino acids in small polypeptides synthesized by nonribosomal peptide synthetases (NRPS) significantly contributes to their biological activity. In these peptides, conversion of L-amino acids to the corresponding D-isomer is catalyzed by specialized NRPS modules that utilize an epimer...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011595t
更新日期：2001-12-25 00:00:00
abstract：:The three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor (RBI) from seeds of ragi (Eleusine coracana Gaertneri) has been determined in solution using multidimensional 1H and 15N NMR spectroscopy. The inhibitor consists of 122 amino acids, with 5 disulfide bridges, and belongs to the plant al...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00026a009
更新日期：1995-07-04 00:00:00
abstract：:The mechanism by which the contractile machinery of muscle is assembled and maintained is not well-understood. Members of the cysteine-rich protein (CRP) family have been implicated in these processes. Three vertebrate CRPs (CRP1-3) that exhibit developmentally regulated muscle-specific expression have been identified...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982036y
更新日期：1999-05-04 00:00:00
abstract：:The effect of calcium ion on the thermal stability of thermolysin has been investigated. The native protein undergoes an irreversible structural change and autolysis at high temperature. Analysis of the calcium ion dependence of the apparent melting temperature observed spectroscopically gives an apparent deltaH of -x...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00650a024
更新日期：1976-03-09 00:00:00
abstract：:Piscidin 1 (Pis-1) is a novel cytotoxic peptide with a cationic alpha-helical structure that was isolated from the mast cells of hybrid striped bass [Silphaduang, U., and Noga, E. J. (2001) Nature 414, 268-269]. Pis-1 is not selective for bacterial versus mammalian cells. In the present study, to develop novel antibio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi062233u
更新日期：2007-03-27 00:00:00
