Abstract:
:The binding of rabbit globin mRNA to the 25-kDa cap binding protein eIF-4E from human erythrocytes was found to be 5.3-fold stronger than the binding of the cap analogue m7GpppG to eIF-4E [Gross et al. (1990) Biochemistry 29, 5008-5012]. In order to investigate whether this effect is due to the longer sequence of nucleotides in globin mRNA or to other features such as cap accessibility or secondary structure, oligoribonucleotide analogues of rabbit alpha-globin mRNA were synthesized by T7 RNA polymerase from a synthetic oligodeoxynucleotide template in the presence of m7GpppG; these oligoribonucleotide analogues possess varying degrees of cap accessibility and secondary structure. Equilibrium association constants for the interaction of these oligoribonucleotides and purified human erythrocyte eIF-4E were obtained from direct fluorescence titration experiments. The data indicate that while the presence of the m7G cap is required for efficient recognition by eIF-4E, the cap need not be completely sterically accessible, since other structural features within the mRNA also influence binding.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Carberry SE,Friedland DE,Rhoads RE,Goss DJdoi
10.1021/bi00120a020subject
Has Abstractpub_date
1992-02-11 00:00:00pages
1427-32issue
5eissn
0006-2960issn
1520-4995journal_volume
31pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Calcium protects a limited number of embryonic chick neural retina cell surface proteins from tryptic cleavage. One glycoprotein of Mr approximately 1.3 X 10(5) and pI approximately 4.8 (gp130) is present in intact retinas and, in the presence of Ca2+, is both resistant to tryptic cleavage and poorly iodinated. When c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00300a016
更新日期:1984-02-28 00:00:00
abstract::PDZ domains are among the most abundant protein modules in the known genomes. Their main function is to provide scaffolds for membrane-associated protein complexes by binding to the cytosolic, C-terminal fragments of receptors, channels, and other integral membrane proteins. Here, using both heteronuclear NMR and sing...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052225y
更新日期:2006-03-21 00:00:00
abstract::Arginine 54 in subunit I of cytochrome c oxidase from Paracoccus denitrificans interacts with the formyl group of heme a. Mutation of this arginine to methionine (R54M) dramatically changes the spectral properties of heme a and lowers its midpoint redox potential [Kannt et al. (1999) J. Biol. Chem. 274, 37974-37981; L...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002948b
更新日期:2001-05-01 00:00:00
abstract::Photosystem II catalyzes the light-driven oxidation of water and reduction of plastoquinone in oxygenic photosynthesis. The manganese stabilizing protein (MSP) of photosystem II is an extrinsic subunit that plays an important role in catalytic activity. This subunit can be extracted and re-bound to the photosystem II ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9724467
更新日期:1998-04-21 00:00:00
abstract::KLHL-12 is a substrate specific adapter protein for a Cul3-Ring ligase complex. It is a member of the Kelch β-propeller domain subclass of Cullin-Ring substrate recognition domains. This E3 ubiquitin ligase complex has many activities, including acting as a negative regulator of the Wnt signaling pathway by mediating ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b01073
更新日期:2020-03-03 00:00:00
abstract::GlgE (EC 2.4.99.16) is an α-maltose 1-phosphate:(1→4)-α-d-glucan 4-α-d-maltosyltransferase of the CAZy glycoside hydrolase 13_3 family. It is the defining enzyme of a bacterial α-glucan biosynthetic pathway and is a genetically validated anti-tuberculosis target. It catalyzes the α-retaining transfer of maltosyl units...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500183c
更新日期:2014-04-22 00:00:00
abstract::The primary structure of the J chain from a human Waldenströms IgM protein has been determined using a combination of automated and conventional Edman degradative procedures. Eighty-five percent of the sequence was established with peptides isolated from tryptic digests of carboxyamidomethylated and citraconylated J c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00635a002
更新日期:1977-08-09 00:00:00
abstract::By means of 2D NMR techniques, all backbone resonances in the 1H NMR spectrum of the single-stranded DNA binding protein encoded by gene V of the filamentous phage IKe have been assigned sequence specifically (at pH 4.6, T = 298 K). In addition, a major part of the side chain resonances could be assigned as well. Anal...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00119a039
更新日期:1992-02-04 00:00:00
abstract::As discussed in the accompanying paper [Markland, W., Ley, A. C., & Ladner, R. C. (1996) Biochemistry 35, 8045-8057], we generated libraries from the first Kunitz domain of human lipoprotein-associated coagulation inhibitor (LACI-D1) using multivalent M13 III display and derived potent inhibitors of human plasmin (PLA...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952629y
更新日期:1996-06-18 00:00:00
abstract::Two novel heme-containing peroxidases, one able to incorporate halogens into aromatic substrates and the other able to remove them, have recently been isolated from marine sources and initially characterized by Chen et al. [(1991) J. Biol. Chem. 266, 23909-23915; (1996) J. Biol. Chem. 271, 4609-4612]. The haloperoxida...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9621371
更新日期:1997-02-25 00:00:00
abstract::The pH-dependent behavior of the ferric forms of two soluble truncations of Rhizobium meliloti FixL, FixL (heme and kinase domains, functional), and FixLN (heme domain) are examined by UV-visible, resonance Raman, and electron paramagnetic resonance spectroscopy. Global analysis of UV-visible data indicates that the p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981439v
更新日期:1998-09-29 00:00:00
abstract::Structural properties of heparan sulfate preparations from hog mucosa and beef lung sources were obtained by application of Smith degradation and nitrous acid reactions. Products formed by these reactions indicated that most of the iduronic acid present in these mucopolysaccharides is ester sulfated, whereas N-sulfate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00629a014
更新日期:1977-05-17 00:00:00
abstract::Gemcitabine 5'-diphosphate (F(2)CDP) is a potent inhibitor of ribonucleotide reductases (RNRs), enzymes that convert nucleotides (NDPs) to deoxynucleotides and are essential for DNA replication and repair. The Escherichia coli RNR, an alpha2beta2 complex, when incubated with 1 equiv of F(2)CDP catalyzes the release of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901590q
更新日期:2009-12-15 00:00:00
abstract::A major regulatory step in the heat-induced transcription of heat shock protein (hsp) genes in eukaryotes is the activation of heat shock factor (HSF). In metazoans and Schizosaccharomyces pombe, HSF is present in unstressed cells but is unable to bind to its target DNA sequence element, the heat shock element (HSE). ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00006a011
更新日期:1995-02-14 00:00:00
abstract::The specificity of valyl-, phenylalanyl-, and tyrosyl-tRNA synthetases from yeast has been examined by a series of stringent tests designed to eliminate the possibility of artefactual interference. Valyl-tRNA synthetase, as well as activating a number of amino acid analogues, will accept alanine, cysteine, isoleucine,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00610a006
更新日期:1978-08-22 00:00:00
abstract::The thermotropic behavior of 20 chemically related glycosphingolipids (GSLs) of high purity, containing neutral and anionic carbohydrate residues in their oligosaccharide chains, was studied by high-sensitivity differential scanning calorimetry. In general, the polar head group of GSLs appears to be one of the major d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00326a003
更新日期:1985-02-26 00:00:00
abstract::Elucidation of the molecular mechanism leading to biomembrane fusion is a challenging issue in current biomedical research in view of its involvement in controlling cellular functions and in mediating various important diseases. According to the generally admitted stalk mechanism described for membrane fusion, negativ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060317x
更新日期:2006-08-01 00:00:00
abstract::Alzheimer's disease is a debilitating neurodegenerative disorder whose pathology has been linked to the aggregation and deposition of the amyloid β-peptide (Aβ) in neural tissue. A truly effective therapeutic agent remains elusive, and attention has recently turned to the use of natural products as effective antiaggre...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300113x
更新日期:2012-07-31 00:00:00
abstract::Cryptophycin-52 (LY355703) is currently undergoing clinical evaluation for cancer chemotherapy. It is a potent suppressor of microtubule dynamics in vitro, and low picomolar concentrations appear to inhibit cancer cell proliferation at mitosis by stabilizing spindle microtubules. In the present study, using [(3)H]cryp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0010827
更新日期:2000-11-21 00:00:00
abstract::A substrate analogue, 6-(difluoromethyl)tryptophan, was developed and characterized for mechanistic investigation of tryptophanase. The utility of this derivative was based on its ability to partition between fluoride elimination and carbon-carbon bond scission during tryptophan metabolism. The non-enzymatic hydrolysi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00221a018
更新日期:1991-02-19 00:00:00
abstract::Tubulin polymerization promoting proteins (TPPPs) constitute a eukaryotic protein family. There are three TPPP paralogs in the human genome, denoted as TPPP1-TPPP3. TPPP1 and TPPP3 are intrinsically unstructured proteins (IUPs) that bind and polymerize tubulin and stabilize microtubules, but TPPP2 does not. Vertebrate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00902
更新日期:2017-02-21 00:00:00
abstract::Complex type N-glycosides of commercial bovine fetuin preparations from pooled fetal calf serum have been shown to contain comparable amounts of Gal4,4,4TRI (see structure A below) and Gal4,4,3TRI (structure B) as major asialo-structures. To investigate whether there is a clear genetic specificity for synthesis of the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00135a024
更新日期:1992-05-26 00:00:00
abstract::The p53 protein family is involved in the control of an intricate network of genes implicated in cell cycle, through to germ line integrity and development. Although the role of p53 is well-established, the intrinsic nature of its homologue p73 has yet to be fully elucidated. Here, the biochemical characterization and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7023207
更新日期:2008-03-11 00:00:00
abstract::Zinc, the catalytically essential metal of angiotensin converting enzyme (ACE), has been replaced by cobalt(II) to give an active, chromophoric enzyme that is spectroscopically responsive to inhibitor binding. Visible absorption spectroscopy and magnetic circular dichroic spectropolarimetry have been used to character...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00397a014
更新日期:1987-11-17 00:00:00
abstract::Cyanobactin heterocyclases share the same catalytic domain (YcaO) as heterocyclases/cyclodehydratases from other ribosomal peptide (RiPPs) biosynthetic pathways. These enzymes process multiple residues (Cys/Thr/Ser) within the same substrate. The processing of cysteine residues proceeds with a known order. We show the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00084
更新日期:2019-04-23 00:00:00
abstract::Fesselin is a proline-rich actin-binding protein that was isolated from avian smooth muscle. Fesselin bundles actin and accelerates actin polymerization by facilitating nucleation. We now show that this polymerization of actin can be regulated by Ca(2+)-calmodulin. Fesselin was shown to bind to immobilized calmodulin ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0487490
更新日期:2004-11-02 00:00:00
abstract::To establish the state of protonation of quinonoid species formed nonenzymically from pyridoxal phosphate (PLP) and diethyl aminomalonate, we have studied absorption spectra of the rapidly established steady-state mixture of species. We have evaluated the formation constant and the spectrum of the mixture of Schiff ba...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00413a050
更新日期:1988-06-28 00:00:00
abstract::Nonenzymatic deamidation of asparagine and glutamine in peptides and proteins is a frequent modification both in vivo and in vitro. The biological effect is not completely understood, but it is often associated with protein degradation and loss of biological function. Here we describe the deamidation of CsgA, the majo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00241
更新日期:2017-06-13 00:00:00
abstract::We find that the photocycle of halorhodopsin (HR) in the presence of nitrate (but not chloride) consists of two parallel series of reactions. The first is essentially the same as that which occurs in the presence of chloride: HRhv----HRK----HRKL----HRL----HRO----HR. The second photocycle, however, which we describe as...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00438a039
更新日期:1989-06-13 00:00:00
abstract::Gastric parietal cell apical membranes must protect the cell from the extremely low pH and wide variations in osmolality of the gastric juice. To characterize the permeability properties of gastric apical membranes, we have measured passive permeabilities to water, protons, NH3, and small nonelectrolytes of membrane v...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00061a002
更新日期:1993-03-16 00:00:00