Levuglandin forms adducts with histone h4 in a cyclooxygenase-2-dependent manner, altering its interaction with DNA.

abstract：:The dimeric two-component system transmitter protein NRII (NtrB) of Escherichia coli, product of glnL (ntrB), controls transcription of nitrogen-regulated genes by catalyzing the phosphorylation and dephosphorylation of the transcription factor NRI (NtrC). Previous studies showed that the PII signal transduction prote...

journal_title：Biochemistry

authors： Jiang P,Atkinson MR,Srisawat C,Sun Q,Ninfa AJ

更新日期：2000-11-07 00:00:00

abstract：:4-Hydroxybenzoyl-CoA reductase (4-HBCR) is a member of the xanthine oxidase (XO) family of molybdenum cofactor containing enzymes and catalyzes the irreversible removal of a phenolic hydroxy group by reduction, yielding benzoyl-CoA and water. In this work the effects of various activity modulating compounds were chara...

journal_title：Biochemistry

authors： Johannes J,Unciuleac MC,Friedrich T,Warkentin E,Ermler U,Boll M

更新日期：2008-04-29 00:00:00

abstract：:The structures of two RNA duplexes, whose sequences correspond to portions of the ColE1 plasmid copy control RNA I and RNA II, have been determined. Crystals containing the 18mers 5'-CA CCGUUGGUAGCGGUGC-3' and 5'-CACCGCUACCAACGGUGC-3' diffract to 1.20 A resolution while those containing the 19mers 5'-GCACCGUUGGUAGCGGU...

journal_title：Biochemistry

authors： Klosterman PS,Shah SA,Steitz TA

更新日期：1999-11-09 00:00:00

abstract：:Retinol (vitamin A alcohol) is a hydrophobic compound and distributes in vivo mainly between binding proteins and cellular membranes. To better clarify the nature of the interactions of retinol with these phases which have a high affinity for it, the thermodynamic parameters of these interactions were studied. The tem...

journal_title：Biochemistry

authors： Noy N,Xu ZJ

更新日期：1990-04-24 00:00:00

abstract：:The neutral glycosphingolipids of hairy cells from a patient with hairy cell leukemia were chemically analyzed by thin-layer and gas-liquid chromatography, mass spectrometry, combined gas chromatography-mass spectrometry, and glycosidase treatment. These cells were found to have compounds containing one to four sugars...

journal_title：Biochemistry

authors： Lee WM,Klock JC,Macher BA

更新日期：1981-10-27 00:00:00

abstract：:Bacillus subtilis CsoR (Bsu CsoR) is a copper-sensing transcriptional repressor that regulates the expression of the copZA operon encoding a copper chaperone and a Cu efflux P-type ATPase, respectively. Bsu CsoR is a homologue of Mycobacterium tuberculosis CsoR (Mtb CsoR), representative of a large Cu(I)-sensing regul...

journal_title：Biochemistry

authors： Ma Z,Cowart DM,Scott RA,Giedroc DP

更新日期：2009-04-21 00:00:00

abstract：:Comparison of available sequences of HLA-A and HLA-B antigens shows that variable positions are predominantly localized in four segments spanning residues 63-85, 105-116, 138-156, and 177-194. The fourth segment is unique in that it contains no differences between antigens of the same locus. Secondary folding of HLA h...

journal_title：Biochemistry

authors： Vega MA,Bragado R,Ezquerra A,López de Castro JA

更新日期：1984-02-28 00:00:00

abstract：:Protein aggregation is associated with a wide range of diseases, and molecular probes that are able to detect a diversity of misfolded protein assemblies are of great importance. The identification of prefibrillar states preceding the formation of well-defined amyloid fibrils is of particular interest both because of ...

journal_title：Biochemistry

authors： Hammarström P,Simon R,Nyström S,Konradsson P,Aslund A,Nilsson KP

更新日期：2010-08-17 00:00:00

abstract：:In an effort to gain greater insight into the molecular mechanism of the electron-transfer reactions of cytochrome b(5), the bovine cytochrome b(5)-horse cytochrome c complex has been investigated by high-resolution multidimensional NMR spectroscopy using (13)C, (15)N-labeled cytochrome b(5) expressed from a synthetic...

journal_title：Biochemistry

authors： Hom K,Ma QF,Wolfe G,Zhang H,Storch EM,Daggett V,Basus VJ,Waskell L

更新日期：2000-11-21 00:00:00

abstract：:The MexA-MexB-OprM efflux pump exports structurally and functionally diverse xenobiotics and confers multi-drug resistance on Pseudomonas aeruginosa cells. The MexB transporter traverses the inner membrane twelve times, bears two large periplasmic domains and has two homologous tandem repeats. To test whether two homo...

journal_title：Biochemistry

authors： Eda S,Yoneyama H,Nakae T

更新日期：2003-06-17 00:00:00

abstract：:Single-tryptophan-containing mutants of low adenylation state Escherichia coli glutamine synthetase (wild type has two tryptophans at positions 57 and 158) have been constructed and studied by multifrequency phase/modulation fluorescence spectroscopy. The W57L mutant (retains tryptophan at residue 158) and the W158S m...

journal_title：Biochemistry

authors： Atkins WM,Stayton PS,Villafranca JJ

更新日期：1991-04-09 00:00:00

abstract：:Sulfenic acid is formed upon oxidation of thiols and is a central intermediate in the redox modulation of an increasing number of proteins. Methods for quantifying or even detecting sulfenic acid are scarce. Herein, the reagent 7-chloro-4-nitrobenz-2-oxa-1,3-diazole was determined not to be suitable as a chromophoric ...

journal_title：Biochemistry

authors： Turell L,Botti H,Carballal S,Ferrer-Sueta G,Souza JM,Durán R,Freeman BA,Radi R,Alvarez B

更新日期：2008-01-08 00:00:00

abstract：:Prodan [6-propionyl-2-(dimethylamine)naphthalene] is a hydrophobic fluorescent probe which is extremely sensitive to both the polarity and the hydrogen-bond donating capacity of the solvent. In binary mixtures of solvents, the hydrogen-bond donating effect on Prodan fluorescence saturates at relatively low concentrati...

journal_title：Biochemistry

authors： Rottenberg H

更新日期：1992-10-06 00:00:00

abstract：:Two major neutral glyceroglucolipids (A and B) have been isolated from lipid extract of human gastric content by the procedure involving column fractionation on DEAE-Sephadex, silicic acid, and thin-layer chromatography. Both glycolipids contained glucose, glyceryl ethers, and fatty acids. The structures of these glyc...

journal_title：Biochemistry

authors： Slomiany BL,Slomiany A,Glass GB

更新日期：1977-09-06 00:00:00

abstract：:The pathological prion protein PrP(Sc) is the only known component of the infectious prion. In cells infected with prions, PrP(Sc) is formed posttranslationally by the refolding of the benign cell surface glycoprotein PrP(C) into an aberrant conformation. The two PrP isoforms possess very different properties, as PrP(...

journal_title：Biochemistry

authors： Tzaban S,Friedlander G,Schonberger O,Horonchik L,Yedidia Y,Shaked G,Gabizon R,Taraboulos A

更新日期：2002-10-22 00:00:00

abstract：:The stereochemistry of proton transfer in the isomerization of [4 beta-2H]-5-androstene-3,17- dione (1d) to 4-androstene-3,17-dione (3) catalyzed by 3-oxo-delta 5-steroid isomerase (KSI) has been reinvestigated. In H2O, approximately 65% of the label is retained in the product (3); of this, one-third is at C-4 and two...

journal_title：Biochemistry

authors： Zawrotny ME,Hawkinson DC,Blotny G,Pollack RM

更新日期：1996-05-21 00:00:00

abstract：:Yah1p, an [Fe 2S 2]-containing ferredoxin located in the matrix of Saccharomyces cerevisiae mitochondria, functions in the synthesis of Fe/S clusters and heme a prosthetic groups. EPR, Mossbauer spectroscopy, and electron microscopy were used to characterize the Fe that accumulates in Yah1p-depleted isolated intact mi...

journal_title：Biochemistry

authors： Miao R,Martinho M,Morales JG,Kim H,Ellis EA,Lill R,Hendrich MP,Münck E,Lindahl PA

更新日期：2008-09-16 00:00:00

abstract：:We have studied the transient kinetics of electron transfer from a positively charged viologen analogue (propylene diquat), reduced by pulsed laser excitation of the deazariboflavin/EDTA system, to the net negatively charged ferredoxins from spinach and Clostridium pasteurianum. Spinach ferredoxin showed monophasic ki...

journal_title：Biochemistry

authors： Navarro JA,Cheddar G,Tollin G

更新日期：1989-07-11 00:00:00

abstract：:The proton transport membrane protein bacteriorhodopsin has been biosynthetically labeled with [methyl-13C]methionine and studied by high-resolution 13C NMR after solubilization in the detergent Triton X-100. The nine methionine residues of bacteriorhodopsin give rise to four well-resolved 13C resonances, two of which...

journal_title：Biochemistry

authors： Seigneuret M,Neumann JM,Levy D,Rigaud JL

更新日期：1991-04-23 00:00:00

abstract：:A rapid, high-yield procedure has been developed for the purification of HPr from the Escherichia coli phosphoenolpyruvate dependent phosphotransferase system. During this procedure, the protein copurifies with a 2500-dalton homopolysaccharide which we have identified as alpha 1-6 glucan. The results of steady-state k...

journal_title：Biochemistry

authors： Dooijewaard G,Roossien FF,Robillard GT

更新日期：1979-07-10 00:00:00

abstract：:The proton-translocating NADH:ubiquinone oxidoreductase of respiratory chains (complex I) contains one flavin mononucleotide and five EPR-detectable iron-sulfur clusters as redox groups. Because of the number of conserved motifs typical for binding iron-sulfur clusters and the high content of iron and acid-labile sulf...

journal_title：Biochemistry

authors： Rasmussen T,Scheide D,Brors B,Kintscher L,Weiss H,Friedrich T

更新日期：2001-05-22 00:00:00

abstract：:It is known that the proapoptotic protein Bax facilitates the formation of pores in bilayers, resulting in the release of proteins from the intermitochondrial space. We demonstrate that another consequence of the interaction of Bax with membranes is an increase in the rate of lipid transbilayer diffusion. We use two i...

journal_title：Biochemistry

authors： Epand RF,Martinou JC,Montessuit S,Epand RM

更新日期：2003-12-16 00:00:00

abstract：:The endogenous phosphoserine residue in porcine pepsinogen has been titrated with use of phosphorus-31 nuclear magnetic resonance (31P NMR). It has an observed pKa2 of 6.7 and a narrow line width (congruent to 10 Hz). The phosphate can be readily removed by an acid phosphatase from potato; however, it is resistant to ...

journal_title：Biochemistry

authors： Williams SP,Bridger WA,James MN

更新日期：1986-10-21 00:00:00

abstract：:Fluorescence lifetimes and fluorescence anisotropy decays of the voltage-sensitive styryl-pyridinium dye RH421 have been measured in the presence of dimyristoylphosphatidylcholine vesicles, the water soluble enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco), and Na+,K(+)-ATPase-containing membrane fragm...

journal_title：Biochemistry

authors： Visser NV,van Hoek A,Visser AJ,Frank J,Apell HJ,Clarke RJ

更新日期：1995-09-19 00:00:00

abstract：:Human telomeres are comprised of d(TTAGGG) repeats that are capable of forming G-quadruplex DNA structures. Ligands that bind to and stabilize these G-quadruplex DNA structures are potential inhibitors of the cancer cell-associated enzyme telomerase. Other potential biological uses of G-quadruplex targeting ligands ha...

journal_title：Biochemistry

authors： Kern JT,Thomas PW,Kerwin SM

更新日期：2002-09-24 00:00:00

abstract：:The respiratory chain complexes can arrange into multienzyme assemblies, so-called supercomplexes. We present the first 3D map of a respiratory chain supercomplex. It was determined by random conical tilt electron microscopy analysis of a bovine supercomplex consisting of complex I, dimeric complex III, and complex IV...

journal_title：Biochemistry

authors： Schäfer E,Dencher NA,Vonck J,Parcej DN

更新日期：2007-11-06 00:00:00

abstract：:The membrane-bound state of the gene 9 minor coat protein of bacteriophage M13 was studied in various membrane-mimicking systems, including organic solvents, detergent micelles, and phospholipid bilayers. For this purpose we determined the conformational and aggregational properties of the chemically synthesized prote...

journal_title：Biochemistry

authors： Houbiers MC,Spruijt RB,Wolfs CJ,Hemminga MA

更新日期：1999-01-19 00:00:00

abstract：:Upon cleavage of the reactive thioester bonds (Cys-949-Glx-952) of tetrameric human alpha 2-macroglobulin (alpha 2M) by methylamine, one sulfhydryl group per alpha 2M subunit is exposed. These identical sulfhydryl group sites were labeled with the thiol-specific nitroxide spin-labels (1-oxy-2,2,5,5-tetramethyl-3-pyrro...

journal_title：Biochemistry

authors： Zhao BL,Musci G,Sugawara Y,Berliner LJ

更新日期：1988-07-12 00:00:00

abstract：:PsbU is a lumenal peripheral protein in the photosystem II (PS II) complex of cyanobacteria and red algae. It is thought that PsbU is replaced functionally by PsbP or PsbQ in plant chloroplasts. After the discovery of PsbP and PsbQ homologues in cyanobacterial PS II [Thornton et al. (2004) Plant Cell 16, 2164-2175], w...

journal_title：Biochemistry

authors： Inoue-Kashino N,Kashino Y,Satoh K,Terashima I,Pakrasi HB

更新日期：2005-09-13 00:00:00

abstract：:Erwinia ligand-gated ion channel (ELIC) is a bacterial homologue of vertebrate pentameric ligand-gated ion channels (pLGICs) and has proven to be a valuable model for understanding the structure and function of this important protein family. There is nevertheless still a question about whether molecular details can be...

journal_title：Biochemistry

authors： Mosesso R,Dougherty DA,Lummis SCR

更新日期：2018-07-10 00:00:00