当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Quantitative characterization of the binding of plasminogen to intact fibrin clots, lysine-sepharose, and fibrin cleaved by plasmin.

Quantitative characterization of the binding of plasminogen to intact fibrin clots, lysine-sepharose, and fibrin cleaved by plasmin.

Abstract：

:The binding of human Glu- and Lys-plasminogens to intact fibrin clots, to lysine-Sepharose, and to fibrin cleaved by plasmin was quantitatively characterized. On intact fibrin clots, there was one strong binding site for Glu-plasminogen with a dissociation constant, Kd, of 25 microM and one strong binding site for Lys-plasminogen with a Kd of 7.9 microM. In both cases, the number of plasminogen binding sites per fibrin monomer was 1. Also, a much weaker binding site for Glu-plasminogen was observed with a Kd of about 350 microM. Limited digestion of fibrin by plasmin created additional binding sites for plasminogen with Kd values similar to the binding of plasminogen to lysine-Sepharose. This was predictable given the observations that plasminogen binds to lysine-Sepharose and can be eluted with epsilon-aminocaproic acid [Deutsch, D.G., & Mertz, E.T. (1970) Science (Washington, D.C.) 170, 1095-1096] and that plasmin preferentially cleaves fibrin at the carboxy side of lysyl residues [Weinstein, M.J., & Doolittle, R.F. (1972) Biochim. Biophys. Acta 258, 577-590], because the structures of the lysyl moiety in lysine-Sepharose and of epsilon-aminocaproic acid are identical with the structure of a COOH-terminal lysyl residue created by plasmin cleavage of fibrin. The Kd for the binding of Glu-plasminogen to lysine-Sepharose was 43 microM and for fibrin partially cleaved by plasmin 48 microM. The Kd for the binding of Lys-plasminogen to lysine-Sepharose was 30 microM. With fibrin partially cleaved by plasmin, there were two types of binding sites for Lys-plasminogen, one with a Kd of 7.6 microM and the other with a Kd of 44 microM.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Bok RA,Mangel WF

doi

10.1021/bi00334a031

subject

Has Abstract

pub_date

1985-06-18 00:00:00

pages

3279-86

issue

13

eissn

0006-2960

issn

1520-4995

journal_volume

24

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Modulating Transmembrane α-Helix Interactions through pH-Sensitive Boundary Residues.

    abstract：:Changes in pH can alter the structure and activity of proteins and may be used by the cell to control molecular function. This coupling can also be used in non-native applications through the design of pH-sensitive biomolecules. For example, the pH (low) insertion peptide (pHLIP) can spontaneously insert into a lipid ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00380

    authors： Ng DP,Deber CM

    更新日期：2016-08-09 00:00:00

  • Observation of manganese(II)-ligand superhyperfine couplings in complexes with proteins by electron spin-echo spectroscopy.

    abstract：:Pulsed electron paramagnetic resonance spectroscopy has been used to detect Mn(II)-ligand superhyperfine couplings in complexes with creatine kinase and in the Mn(II) metalloprotein concanavalin A. Electron spin-echo envelopes from Mn(II), bound in these complexes, are modulated by superhyperfine interactions between ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00367a046

    authors： LoBrutto R,Smithers GW,Reed GH,Orme-Johnson WH,Tan SL,Leigh JS Jr

    更新日期：1986-09-23 00:00:00

  • Ganglioside G(M1)-mediated amyloid-beta fibrillogenesis and membrane disruption.

    abstract：:There is increasing evidence that a class of cell membrane glycolipids, gangliosides, can mediate the fibrillogenesis and toxicity of Alzheimer's disease amyloid-beta peptide (Abeta). Using lipid monolayers and vesicles as model membranes, we measured the insertion of Abeta into 1,2-dipalmitoyl-sn-glycero-3-phosphocho...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi062177x

    authors： Chi EY,Frey SL,Lee KY

    更新日期：2007-02-20 00:00:00

  • Structural RNA mimetics: N3'-->P5' phosphoramidate DNA analogs of HIV-1 RRE and TAR RNA form A-type helices that bind specifically to Rev and Tat-related peptides.

    abstract：:An attractive strategy for the development of anti-retroviral drugs is the exploration of compounds that mimic RNA control regions of the viral genome and act as "decoys" to sequester viral gene regulatory proteins. Decoys consisting of RNA, however, are chemically unstable and readily degraded by cellular nucleases. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961980w

    authors： Rigl CT,Lloyd DH,Tsou DS,Gryaznov SM,Wilson WD

    更新日期：1997-01-21 00:00:00

  • A monosaccharide is bound to the sodium pump alpha-subunit.

    abstract：:We have recently reported that the Na pump alpha-subunit has cytosolic-oriented oligosaccharides which were sensitive to cleavage by an enzyme specific for hydrolysis of N-linked glycans [Pedemonte et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 9789-9793]. We now describe experiments that characterize the saccharides...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00158a008

    authors： Pedemonte CH,Kaplan JH

    更新日期：1992-11-03 00:00:00

  • Dual calcium ion regulation of calcineurin by calmodulin and calcineurin B.

    abstract：:The dependence of calcineurin on Ca2+ for activity is the result of the concerted action of calmodulin, which increases the turnover rate of the enzyme and modulates its response to Ca2+ transients, and of calcineurin B, which decreases the Km of the enzyme for its substrate. The calmodulin-stimulated protein phosphat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00188a015

    authors： Stemmer PM,Klee CB

    更新日期：1994-06-07 00:00:00

  • Substitutions of coenzyme-binding, nonpolar residues improve the low-temperature activity of thermophilic dehydrogenases.

    abstract：:Although enzymes of thermophilic organisms are often very resistant to thermal denaturation, they are usually less active than their mesophilic or psychrophilic homologues at moderate or low temperatures. To explore the structural features that would improve the activity of a thermophilic enzyme at less than optimal t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200925f

    authors： Hayashi S,Akanuma S,Onuki W,Tokunaga C,Yamagishi A

    更新日期：2011-10-11 00:00:00

  • Substrate analogs as mechanistic probes for the bifunctional chorismate synthase from Neurospora crassa.

    abstract：:Analogs of EPSP (4-8) have been prepared, and their activity as substrates for the chorismate synthase from Neurospora crassa has been characterized kinetically. The enzyme appears to show strict discrimination against substitution at the Z-position of the enol ether side chain as well as against substitution at the S...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00251a019

    authors： Lauhon CT,Bartlett PA

    更新日期：1994-11-29 00:00:00

  • Solution structure of a band 3 peptide inhibitor bound to aldolase: a proposed mechanism for regulating binding by tyrosine phosphorylation.

    abstract：:Human erythrocyte band 3 inhibits glycolytic enzymes, including aldolase, by binding these cytoplasmic enzymes at its N-terminus. Phosphorylation of Y8 disrupts inhibition, and there is evidence that in vivo glycolysis levels in erythrocytes are regulated in part by a phosphorylation/dephosphorylation signaling pathwa...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00051a005

    authors： Schneider ML,Post CB

    更新日期：1995-12-26 00:00:00

  • Structure of the O-specific, sialic acid containing polysaccharide chain and its linkage to the core region in lipopolysaccharide from Hafnia alvei strain 2 as elucidated by chemical methods, gas-liquid chromatography/mass spectrometry, and 1H NMR spectro

    abstract：:Mild acid hydrolysis of Hafnia alvei strain 2 lipopolysaccharide released no O-specific polysaccharide but instead gave a monomeric octasaccharide repeating unit with N-acetylneuraminic acid as the reducing terminus. In addition, a dimer of the octasaccharide repeating unit, and also a decasaccharide composed of a fra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00234a027

    authors： Gamian A,Romanowska E,Dabrowski U,Dabrowski J

    更新日期：1991-05-21 00:00:00

  • Nearest neighbor analysis of heparin: identification and quantitation of the products formed by selective depolymerization procedures.

    abstract：:Heparin was carboxyl-reduced with sodium boro[3H]hydride and converted to a mixture of oligosaccharides by treatment with nitrous acid at pH 2. The oligosaccharide mixture was aldehyde-reduced with sodium boro[3H]hydride and the mixture of products, labeled both in the hexoses formed in the carboxyl-reduction step and...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00663a006

    authors： Shively JE,Conrad HE

    更新日期：1976-09-07 00:00:00

  • Specific substitution into the anticodon loop of yeast tyrosine transfer RNA.

    abstract：:The aminoacylation kinetics of 19 different variants of yeast tRNATyr with nucleotide substitutions in positions 33-35 were determined. Substitution of the conserved uridine-33 does not alter the rate of aminoacylation. However, substitution of the anticodon position 34 or position 35 reduces Km from 2- to 10-fold and...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00367a072

    authors： Bare LA,Uhlenbeck OC

    更新日期：1986-09-23 00:00:00

  • Crystal structure of the catalytic domain of a thermophilic endocellulase.

    abstract：:One way to improve the economic feasibility of biomass conversion is to enhance the catalytic efficiency of cellulases through protein engineering. This requires that high-resolution structures of cellulases be available. Here we present the structure of E2cd, the catalytic domain of the thermophilic endocellulase E2 ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00089a006

    authors： Spezio M,Wilson DB,Karplus PA

    更新日期：1993-09-28 00:00:00

  • Site-directed mutagenesis identifies catalytic residues in the active site of Escherichia coli phosphofructokinase.

    abstract：:Six active site mutants of Escherichia coli phosphofructokinase have been constructed and characterized using steady-state kinetics. All but one of the mutants (ES222) have significantly lower maximal activity, implicating these residues in the catalytic process. Replacement of Asp127, the key catalytic residue in the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00153a017

    authors： Berger SA,Evans PR

    更新日期：1992-09-29 00:00:00

  • Vinca alkaloid-induced tubulin spiral formation correlates with cytotoxicity in the leukemic L1210 cell line.

    abstract：:The ability of a class of C-20' modified vinca alkaloid congeners to induce tubulin spiral formation was investigated relative to their ability to inhibit microtubule assembly, their cytotoxicity against a leukemic cell line, L1210, and their measured and calculated partition coefficients. These studies were prompted ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001038r

    authors： Lobert S,Fahy J,Hill BT,Duflos A,Etievant C,Correia JJ

    更新日期：2000-10-03 00:00:00

  • Interaction of ribosomal protein S1 and initiation factor IF3 with the 3' major domain and the decoding site of the 30S subunit of Escherichia coli.

    abstract：:We have studied the effect of the binding of ribosomal protein S1 and initiation factor IF3 on the accessibility of nucleotide residues 584-1506 in the small subunit of the Escherichia coli ribosome. Protein S1 strongly decreases RNase V1 attack at G1164, in hairpin 40 of the 3' major domain, and weakly decreases DMS ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00112a007

    authors： Laughrea M,Tam J

    更新日期：1991-12-03 00:00:00

  • Interaction of a paramagnetic analogue of oxaloacetate with citrate synthase.

    abstract：:Electron paramagnetic resonance studies have indicated that nitrosodisulfonate binds to pig heart citrate synthase. Titration of the enzyme with nitrosodisulfonate revealed several binding sites for the probe per subunit with one site (KD approximately 0.1 mM) having a greater affinity than the others. The substrate, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00584a028

    authors： Weidman SW,Drysdale GR

    更新日期：1979-08-21 00:00:00

  • Conformational changes in human hepatitis C virus NS3 protease upon binding of product-based inhibitors.

    abstract：:One of the most promising approaches to anti-hepatitis C virus drug discovery is the development of inhibitors of the virally encoded protease NS3. This chymotrypsin-like serine protease is essential for the maturation of the viral polyprotein, and processing requires complex formation between NS3 and its cofactor NS4...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991220w

    authors： Bianchi E,Orrù S,Dal Piaz F,Ingenito R,Casbarra A,Biasiol G,Koch U,Pucci P,Pessi A

    更新日期：1999-10-19 00:00:00

  • A deuterium and phosphorus-31 nuclear magnetic resonance study of the interaction of melittin with dimyristoylphosphatidylcholine bilayers and the effects of contaminating phospholipase A2.

    abstract：:The interaction of bee venom melittin with dimyristolphosphatidylcholine (DMPC) selectively deuteriated in the choline head group has been studied by deuterium and phosphorus-31 nuclear magnetic resonance (NMR) spectroscopy. The action of residual phospholipase A2 in melittin samples resulted in mixtures of DMPC and i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00392a033

    authors： Dempsey CE,Watts A

    更新日期：1987-09-08 00:00:00

  • A glutamic acid specific serine protease utilizes a novel histidine triad in substrate binding.

    abstract：:Proteases specific for cleavage after acidic residues have been implicated in several disease states, including epidermolysis, inflammation, and viral processing. A serine protease with specificity toward glutamic acid substrates (Glu-SGP) has been crystallized in the presence of a tetrapeptide ligand and its structur...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00094a001

    authors： Nienaber VL,Breddam K,Birktoft JJ

    更新日期：1993-11-02 00:00:00

  • Mechanism of glutamate transport in Escherichia coli B. 1. Proton-dependent and sodium ion dependent binding of glutamate to a glutamate carrier in the cytoplasmic membrane.

    abstract：:Specific binding of glutamate to its carrier was investigated by using cytoplasmic membrane vesicles prepared from Escherichia coli B. The binding activity was specifically affected by the Na+ and H+ concentrations of the medium. Cytoplasmic membrane vesicles from the mutant strain 36-39 that is defective in the Na+-d...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00277a033

    authors： Fujimura T,Yamato I,Anraku Y

    更新日期：1983-04-12 00:00:00

  • Crotonase-catalyzed beta-elimination is concerted: a double isotope effect study.

    abstract：:Determining the sequence of bond cleavages, and consequently the nature of intermediates, in enzyme-catalyzed reactions is a major goal of mechanistic enzymology. When significant primary isotope effects on V/K are observed for two different bond cleavages, both bonds may be broken in the same transition state or they...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00238a013

    authors： Bahnson BJ,Anderson VE

    更新日期：1991-06-18 00:00:00

  • Interaction of tRNAs and of phosphorothioate-substituted nucleic acids with an organomercurial. Probing the chemical environment of thiolated residues by affinity electrophoresis.

    abstract：:The interactions of 4-thiouridine and 5-[(methylamino)methyl]-2-thiouridine in tRNA and of phosphorothioate esters in nucleic acids with an organomercurial have been investigated. For this purpose, an affinity electrophoretic system has been developed in which the mercury derivative has been covalently immobilized in ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00410a048

    authors： Igloi GL

    更新日期：1988-05-17 00:00:00

  • Investigation of the structure of bovine erythrocyte superoxide dismutase by 1H nuclear magnetic resonance spectroscopy.

    abstract：:The 270-MHz 1H nuclear magnetic resonance spectra of the apo, copper(I)-zinc(II), and copper(II)-zinc(II) forms of bovine erythrocyte superoxide dismutase (EC 1.15.1.1) are reported, and assignments of resonances to ten amino acid residues are proposed. The data require that at least four and probably six histidine re...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00633a003

    authors： Cass AE,Hill AO,Smith BE,Bannister JV,Bannister WH

    更新日期：1977-07-12 00:00:00

  • Structural investigation of the binding of a herpesviral protein to the SH3 domain of tyrosine kinase Lck.

    abstract：:Herpesvirus saimiri codes for a tyrosine kinase interacting protein (Tip) that interacts with both the SH3 domain and the kinase domain of the T-cell-specific tyrosine kinase Lck via two separate motifs. The activation of Lck by Tip is considered as a key event in the transformation of human T-lymphocytes during herpe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015986j

    authors： Schweimer K,Hoffmann S,Bauer F,Friedrich U,Kardinal C,Feller SM,Biesinger B,Sticht H

    更新日期：2002-04-23 00:00:00

  • Structure and function of DNA photolyase.

    abstract：:Cyclobutane pyrimidine dimers (Pyr < > Pyr) are the major DNA photoproducts induced by the UV component of solar radiation. Photoreactivating enzyme (DNA photolyase) repairs DNA by utilizing the energy of visible light to break the cyclobutane ring of the dimer. Photolyases are monomeric proteins of 50-60 kDa with sto...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi00167a001

    authors： Sancar A

    更新日期：1994-01-11 00:00:00

  • Lipid binding by fragments of apolipoprotein C-III-1 obtained by thrombin cleavage.

    abstract：:We have used thrombin to cleave apolipoprotein C-III-1 into two fragments constituting residues 1-40 (apoLP-C-III-A) and 41-79 (apoLP-C-III-B). The lipid binding properties of these fragments with dimyristoyl- and 1-palmitoyl-2-oleoylphosphatidylcholines have been determined using circular dichroic and intrinsic trypt...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00644a004

    authors： Sparrow JT,Pownall HJ,Hsu FJ,Blumenthal LD,Culwell AR,Gotto AM

    更新日期：1977-12-13 00:00:00

  • The vinblastine binding site adopts high- and low-affinity conformations during a transport cycle of P-glycoprotein.

    abstract：:Conceptually one may envisage that substrate binding sites on the ABC transporter P-gp cycle between high- and low-affinity conformations in response to signals arising from nucleotide hydrolysis to effect active transport. A radioligand binding assay was used to characterize the interaction of [3H]vinblastine with P-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011211z

    authors： Martin C,Higgins CF,Callaghan R

    更新日期：2001-12-25 00:00:00

  • X-ray structures of Torpedo californica acetylcholinesterase complexed with (+)-huperzine A and (-)-huperzine B: structural evidence for an active site rearrangement.

    abstract：:Kinetic and structural data are presented on the interaction with Torpedo californica acetylcholinesterase (TcAChE) of (+)-huperzine A, a synthetic enantiomer of the anti-Alzheimer drug, (-)-huperzine A, and of its natural homologue (-)-huperzine B. (+)-Huperzine A and (-)-huperzine B bind to the enzyme with dissociat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi020151+

    authors： Dvir H,Jiang HL,Wong DM,Harel M,Chetrit M,He XC,Jin GY,Yu GL,Tang XC,Silman I,Bai DL,Sussman JL

    更新日期：2002-09-03 00:00:00

  • Pig liver phosphomevalone kinase. 1. Purification and properties.

    abstract：:Pig liver phosphomevalonate kinase (EC 2.7.4.2) has been purified to homogeneity as shown by polyacrylamide gel electrophoresis. The molecular weight estimates range from 21,000 to 22,500. Each molecule is composed of one polypeptide chain. The presence of SH-containing reagents is essential for the preservation of en...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00552a003

    authors： Bazaes S,Beytía E,Jabalquinto AM,Solís de Ovando F,Gómez I,Eyzaguirre J

    更新日期：1980-05-27 00:00:00