Structure of the O-specific, sialic acid containing polysaccharide chain and its linkage to the core region in lipopolysaccharide from Hafnia alvei strain 2 as elucidated by chemical methods, gas-liquid chromatography/mass spectrometry, and 1H NMR spectro

abstract：:Cellular toxicity resulting from nucleation-dependent polymerization of amyloid beta-peptide (Abeta) is considered to be a major and possibly the primary component of Alzheimer's disease (AD). Inhibition of Abeta polymerization has thus been identified as a target for the development of therapeutic agents for the trea...

journal_title：Biochemistry

authors： Findeis MA,Musso GM,Arico-Muendel CC,Benjamin HW,Hundal AM,Lee JJ,Chin J,Kelley M,Wakefield J,Hayward NJ,Molineaux SM

更新日期：1999-05-25 00:00:00

abstract：:Syk, a nonreceptor protein-tyrosine kinase, is activated by both oxidative and osmotic stress and plays different roles in the transduction of stress signals. In this study, the regulation of oxidative and osmotic stress induced Syk activation was investigated utilizing Syk-negative DT40 cells, expressing various Syk ...

journal_title：Biochemistry

authors： Qin S,Kurosaki T,Yamamura H

更新日期：1998-04-21 00:00:00

abstract：:Nicotinamide adenine dinucleotide (NAD) dependent urocanase (4'-imidazolone-5'-propionate hydro-lyase, EC 4.2.1.49) from Pseudomonas putida was found to catalyze an exchange reaction between solvent and the 4'-hydrogen of urocanate or imidazolepropionate at a rate faster than that of overall deuterium was compared to ...

journal_title：Biochemistry

authors： Egan RM,Matherly LH,Phillips AT

更新日期：1981-01-06 00:00:00

abstract：:The difference spectra of binary and ternary complexes of horse liver alcohol dehydrogenase with oxidized and reduced nicotinamide adenine dinucleotides, nicotinamide 1,N6-ethenoadenine dinucleotide, and adenosine diphosphate ribose along with a number of substrate analogues have been measured. These spectra bear a ve...

journal_title：Biochemistry

authors： Subramanian S,Ross JB,Ross PD,Brand L

更新日期：1981-07-07 00:00:00

abstract：:The crystal structure of a ternary complex of the alcohol dehydrogenase from the archaeon Sulfolobus solfataricus (SsADH) has been determined at 2.3 A. The asymmetric unit contains a dimer with a NADH and a 2-ethoxyethanol molecule bound to each subunit. The comparison with the apo structure of the enzyme reveals that...

journal_title：Biochemistry

authors： Esposito L,Bruno I,Sica F,Raia CA,Giordano A,Rossi M,Mazzarella L,Zagari A

更新日期：2003-12-16 00:00:00

abstract：:HTC cells have been labeled by short exposures to [3H]thymidine in order to identify newly synthesized DNA. By either isolating nuclei directly or isolating them after an extensive fixation with formaldehyde, we have been able to identify two phases in the maturation process of newly replicated chromatin. The first ph...

journal_title：Biochemistry

authors： Smith PA,Jackson V,Chalkley R

更新日期：1984-03-27 00:00:00

abstract：:If cytochrome c oxidase is subjected to pressure during the aerobic steady state, large spectral changes are apparent. These seem to be associated with the inhibition of electron transport within the oxidase. The volume change for the transition is about 80 mL/mol. When the oxidase in the aerobic steady state, with po...

journal_title：Biochemistry

authors： Kornblatt JA,Hui Bon Hoa G,Heremans K

更新日期：1988-07-12 00:00:00

abstract：:Phenylcarbene and adamantylidene have been generated photochemically from the corresponding diazirines within lipid bilayers. Reasonable yields of labeled fatty acid side chains have been observed. The products have been characterized by gas chromatography-mass spectrometry and derive both from the insertion of the c...

journal_title：Biochemistry

authors： Bayley H,Knowles JR

更新日期：1978-06-13 00:00:00

abstract：:The origin of the "S3" EPR signal from calcium-depleted photosystem 2 samples has been investigated. This signal is observed after freezing samples under illumination and has been assigned to an interaction between the manganese cluster and an oxidized histidine radical [Boussac et al. (1990) Nature 347; 303-306]. In ...

journal_title：Biochemistry

authors： Hallahan BJ,Nugent JH,Warden JT,Evans MC

更新日期：1992-05-19 00:00:00

abstract：:The BARD1 N-terminal RING domain binds BRCA1 while the BARD1 C-terminal ankyrin and tandem BRCT repeat domains bind CstF-50 to modulate mRNA processing and RNAP II stability in response to DNA damage. Here we characterize the BARD1 structural biochemistry responsible for CstF-50 binding. The crystal structure of the B...

journal_title：Biochemistry

authors： Edwards RA,Lee MS,Tsutakawa SE,Williams RS,Nazeer I,Kleiman FE,Tainer JA,Glover JN

更新日期：2008-11-04 00:00:00

abstract：:An organic solvent soluble polypeptide has been isolated from photoreceptor complexes and chromatophores of Rhodospirillum rubrum. After extraction of the protein from lyophilized samples with 1:1 chloroform-methanol, it was purified by column chromatography. Its isoelectric point determined by isoelectric focusing wa...

journal_title：Biochemistry

authors： Tonn SJ,Gogel GE,Loach PA

更新日期：1977-03-08 00:00:00

abstract：:PsbO, the photosystem II manganese stabilizing protein, contains an aspartate residue [Asp157 (spinach numbering)], which is highly conserved in eukaryotic and prokaryotic PsbOs. The homology model of the PSII-bound conformation of spinach PsbO presented here positions Asp157 in the large flexible loop of the protein....

journal_title：Biochemistry

authors： Popelkova H,Commet A,Yocum CF

更新日期：2009-12-22 00:00:00

abstract：:Several recent studies have provided evidence that LIN28, a cytoplasmic RNA-binding protein, inhibits the biogenesis of members of the let-7 microRNA family at the Dicer step in both mammals and Caenorhabditis elegans. However, the precise mechanism of inhibition is still poorly understood. Here we report on an in vit...

journal_title：Biochemistry

authors： Lightfoot HL,Bugaut A,Armisen J,Lehrbach NJ,Miska EA,Balasubramanian S

更新日期：2011-09-06 00:00:00

abstract：:A peptide consisting of the first 21 residues of human myelin basic protein (MBP) was synthesized. The N-terminal alanine of portions was blocked in separate experiments with alkyl carboxylates varying in size from 2 to 10 carbon atoms. The effects of these different alkyl carboxylates at the N-terminus on the seconda...

journal_title：Biochemistry

authors： Costentino M,Pritzker L,Boulias C,Moscarello MA

更新日期：1994-04-12 00:00:00

abstract：:The involvement of calmodulin in the proliferation of Chinese hamster embryo fibroblast cells has been studied with a specific monoclonal antibody to calmodulin. We observed that calmodulin levels increase 2-fold in the late G1 period in these cells, and this coincides with the increase in DNA polymerase alpha activit...

journal_title：Biochemistry

authors： Reddy GP,Reed WC,Sheehan E,Sacks DB

更新日期：1992-11-03 00:00:00

abstract：:The phosphorylation of (Na+, K+)ATPase from the electric organ of the electric eel is dependent on Mg2+. The amount of phosphoenzyme formed was increased by K+ and decreased by Na+. Kinetic analyses indicate that a ternary complex of ATPase, Pi and Mg2+ is formed prior to phosphorylation of the protein. Calorimetric s...

journal_title：Biochemistry

authors： Kuriki Y,Halsey J,Biltonen R,Racker E

更新日期：1976-11-16 00:00:00

abstract：:Moderate pressure accelerates hydride transfer catalyzed by yeast alcohol dehydrogenase, indicative of a large negative volume of activation [Cho and Northrop (1999) Biochemistry 38, 7470-7475]. A comparison of the effects of pressure on the oxidation of normal versus dideuteriobenzyl alcohol generates a monophasic de...

journal_title：Biochemistry

authors： Northrop DB,Cho YK

更新日期：2000-03-07 00:00:00

abstract：:Residual dipolar couplings (RDCs) were used as restraints in fully solvated molecular dynamics simulations of reduced substrate- and carbonmonoxy-bound cytochrome P450(cam) (CYP101A1), a 414-residue soluble monomeric heme-containing camphor monooxygenase from the soil bacterium Pseudomonas putida. The (1)D(NH) residua...

journal_title：Biochemistry

authors： Asciutto EK,Dang M,Pochapsky SS,Madura JD,Pochapsky TC

更新日期：2011-03-15 00:00:00

abstract：:A detailed structural study of the prokaryotic sodium/galactose transporter (vSGLT) from Vibrio parahaemolyticus using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy reveals stepwise increases in alpha-helicity upon binding of sodium and D-galactose. These increases in helicity correlat...

journal_title：Biochemistry

authors： le Coutre J,Turk E,Kaback HR,Wright EM

更新日期：2002-06-25 00:00:00

abstract：:Lysine 2,3-aminomutase from Clostridia catalyzes the interconversion of L-lysine and L-beta-lysine. The enzyme contains iron-sulfur clusters and is activated by pyridoxal 5'-phosphate and S-adenosylmethionine, all of which participate in catalysis. Current spectroscopic evidence implicates two substrate-based organic ...

journal_title：Biochemistry

authors： Chang CH,Ballinger MD,Reed GH,Frey PA

更新日期：1996-08-27 00:00:00

abstract：:As part of our studies on the molecular mechanism of mutation [Chambers, R. W. (1982) in Molecular and Cellular Mechanisms of Mutagenesis (Lemontt, J. F., & Generoso, W. M., Eds.) pp 121-145, Plenum, New York and London], we wanted to prepare specific oligonucleotides carrying O2- or O4-alkylthymidine residues. Since ...

journal_title：Biochemistry

authors： Borowy-Borowski H,Chambers RW

更新日期：1989-02-21 00:00:00

abstract：:The ras oncogene products (H-Ras, K-Ras, and N-Ras) have been regarded as some of the most promising targets for anticancer drug discovery because their activating mutations are frequently found in human cancers. Nonetheless, molecular targeted therapy for them is currently unavailable. Here, we report the discovery o...

journal_title：Biochemistry

authors： Matsumoto S,Hiraga T,Hayashi Y,Yoshikawa Y,Tsuda C,Araki M,Neya M,Shima F,Kataoka T

更新日期：2018-09-11 00:00:00

abstract：:Binding of substrate to ornithine 4,5-aminomutase (OAM) and methylmalonyl-CoA mutase (MCM) leads to the formation of an electrostatic interaction between a conserved glutamate side chain and the adenosyl ribose of the adenosylcobalamin (AdoCbl) cofactor. The contribution of this residue (Glu338 in OAM from Clostridium...

journal_title：Biochemistry

authors： Makins C,Pickering AV,Mariani C,Wolthers KR

更新日期：2013-02-05 00:00:00

abstract：:A cationic, copper(II)-containing ligand, derived from bulky 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)porphyrin, Cu(T4), and two sterically friendlier forms, [trans-5,15-di(N-methylpyridinium-4-yl)porphyrinato]copper(II), Cu(tD4), and [cis-5,10-di(N-methylpyridinium-4-yl)porphyrinato]copper(II), Cu(cD4), bind to DN...

journal_title：Biochemistry

authors： Briggs BN,Gaier AJ,Fanwick PE,Dogutan DK,McMillin DR

更新日期：2012-09-25 00:00:00

abstract：:Although host protein synthesis is preferentially inhibited, there is a steady decline in the ability of Chinese hamster ovary (CHO) cells infected with vesicular stomatitis virus (VSV) to synthesize both host and viral proteins. We previously reported finding an mRNA-ribonucleoprotein particle (mRNP) that contained a...

journal_title：Biochemistry

authors： Rosen CA,Siekierka J,Ennis HL,Cohen PS

更新日期：1984-05-22 00:00:00

abstract：:The first committed step of fatty acid and polyketides biosynthesis, the biotin-dependent carboxylation of an acyl-CoA, is catalyzed by acyl-CoA carboxylases (ACCases) such as acetyl-CoA carboxylase (ACC) and propionyl-CoA carboxylase (PCC). ACC and PCC in Streptomyces coelicolor are homologue multisubunit complexes t...

journal_title：Biochemistry

authors： Arabolaza A,Shillito ME,Lin TW,Diacovich L,Melgar M,Pham H,Amick D,Gramajo H,Tsai SC

更新日期：2010-08-31 00:00:00

abstract：:We have investigated the kinetic and thermodynamic properties of carbon monoxide binding to the fully reduced quinol oxidase (cytochrome aa(3)) from the hyperthermophilic archaeon Acidianus ambivalens. After flash photolysis of CO from heme a(3), the complex recombines with an apparent rate constant of approximately 3...

journal_title：Biochemistry

authors： Aagaard A,Gilderson G,Gomes CM,Teixeira M,Brzezinski P

更新日期：1999-08-03 00:00:00

abstract：:The periplasmic protein CusF, as a part of the CusCFBA efflux complex, plays a role in resistance to elevated levels of copper and silver in Escherichia coli. Although homologues have been identified in other Gram-negative bacteria, the substrate of CusF and its precise role in metal resistance have not been described...

journal_title：Biochemistry

authors： Kittleson JT,Loftin IR,Hausrath AC,Engelhardt KP,Rensing C,McEvoy MM

更新日期：2006-09-19 00:00:00

abstract：:Human type-alpha transforming growth factor (hTGF alpha) is a small mitogenic protein containing 50 amino acids and three disulfide bonds. It has both sequence and structural homology with epidermal growth factor (EGF). While the three-dimensional structures of hTGF alpha and other EGF-like proteins have been studied ...

journal_title：Biochemistry

authors： Li YC,Montelione GT

更新日期：1995-02-28 00:00:00

abstract：:The binding of 125I-labeled ovine follitropin (oFSH) and 125I-labeled deglycosylated ovine follitropin (DG-oFSH) to porcine granulosa cell membranes was studied at equilibrium and nonequilibrium binding conditions and statistically analyzed. Saturation and competition binding experiments revealed homogeneity in the po...

journal_title：Biochemistry

authors： Sebok K,De Lean A,Sairam MR

更新日期：1987-06-16 00:00:00