当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Acylation of myelin basic protein peptide 1-21 with alkyl carboxylates 2-10 carbons long affects secondary structure and posttranslational modification.

Acylation of myelin basic protein peptide 1-21 with alkyl carboxylates 2-10 carbons long affects secondary structure and posttranslational modification.

Abstract：

:A peptide consisting of the first 21 residues of human myelin basic protein (MBP) was synthesized. The N-terminal alanine of portions was blocked in separate experiments with alkyl carboxylates varying in size from 2 to 10 carbon atoms. The effects of these different alkyl carboxylates at the N-terminus on the secondary structure was studied by circular dichroism (250-190 nm). In water, the spectra of the unblocked peptide suggested unordered structure with large negative ellipticities at 198 nm. Addition of an acetyl group altered the magnitude of [theta]198 from -21856 +/- 2319 to -11095 +/- 1000 deg cm2 dmol-1, suggesting a significant increase in ordered structure. When peptides with longer alkyl carboxylates, acylated at the N-termini, were studied, the magnitude of theta 198 approached that of the unblocked peptide but greater negative ellipticities were observed for the C8 and C10 alkyl carboxylates. The theta 222 values were generally low (-1803 +/- 463) but increased with increasing length of the alkyl carboxylate to about -3200 deg cm2 dmol-1, suggesting that little alpha-helical structure was present. The spectra were also taken in lipid-mimetic solvents, including 2-propanol, methanol, and lysophosphatidylglycerol (lysoPG). In general the theta 198 and theta 222 values were suggestive of increased structure in these environments compared to water. In 90% 2-propanol the theta 198 of the unblocked peptide did not change when an acetyl group was added to the N-terminus (9088 compared to 8477 deg cm2 dmol-1). Addition of longer alkyl carboxylates correlated with larger, negative ellipticities.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Costentino M,Pritzker L,Boulias C,Moscarello MA

doi

10.1021/bi00180a008

subject

Has Abstract

pub_date

1994-04-12 00:00:00

pages

4155-62

issue

14

eissn

0006-2960

issn

1520-4995

journal_volume

33

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Molecular Basis for Allosteric Inhibition of GTP-Bound H-Ras Protein by a Small-Molecule Compound Carrying a Naphthalene Ring.

    abstract：:The ras oncogene products (H-Ras, K-Ras, and N-Ras) have been regarded as some of the most promising targets for anticancer drug discovery because their activating mutations are frequently found in human cancers. Nonetheless, molecular targeted therapy for them is currently unavailable. Here, we report the discovery o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00680

    authors： Matsumoto S,Hiraga T,Hayashi Y,Yoshikawa Y,Tsuda C,Araki M,Neya M,Shima F,Kataoka T

    更新日期：2018-09-11 00:00:00

  • Measuring RNA-Ligand Interactions with Microscale Thermophoresis.

    abstract：:In recent years, there has been dramatic growth in the study of RNA. RNA has gone from being known as an intermediate in the central dogma of molecular biology to a molecule with a large diversity of structure and function that is involved in all aspects of biology. As new functions are rapidly discovered, it has beco...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b01141

    authors： Moon MH,Hilimire TA,Sanders AM,Schneekloth JS Jr

    更新日期：2018-08-07 00:00:00

  • Kinetic and Spectroscopic Characterization of the Catalytic Ternary Complex of Tryptophan 2,3-Dioxygenase.

    abstract：:The first step of the kynurenine pathway for l-tryptophan (l-Trp) degradation is catalyzed by heme-dependent dioxygenases, tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase. In this work, we employed stopped-flow optical absorption spectroscopy to study the kinetic behavior of the Michaelis complex of C...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00179

    authors： Geng J,Weitz AC,Dornevil K,Hendrich MP,Liu A

    更新日期：2020-08-04 00:00:00

  • Self-assembly of aqueous bilirubin ditaurate, a natural conjugated bile pigment, to contraposing enantiomeric dimers and M(-) and P(+) tetramers and their selective hydrophilic disaggregation by monomers and micelles of bile salts.

    abstract：:The solution behavior of bilirubin ditaurate (BDT), the first naturally occurring conjugated bile pigment to be physically and chemically characterized, was assessed in aqueous solution and in monomeric and micellar solutions of common taurine-conjugated bile salts (BS). Analytical ultracentrifugation revealed that BD...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi501251v

    authors： Neubrand MW,Carey MC,Laue TM

    更新日期：2015-02-24 00:00:00

  • Reconstitution of a defunct glycolytic pathway via recruitment of ambiguous sugar kinases.

    abstract：:During a recent investigation of the persistence of substrate ambiguity in contemporary enzymes, we identified three distinct ambiguous sugar kinases embedded within the modern Escherichia coli genome [Miller, B. G., and Raines, R. T. (2004) Biochemistry 43, 6387-6392]. These catalysts are the YajF, YcfX, and NanK pol...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0506268

    authors： Miller BG,Raines RT

    更新日期：2005-08-16 00:00:00

  • Amino Acid Selective 13C Labeling and 13C Scrambling Profile Analysis of Protein α and Side-Chain Carbons in Escherichia coli Utilized for Protein Nuclear Magnetic Resonance.

    abstract：:Amino acid selective isotope labeling is an important nuclear magnetic resonance technique, especially for larger proteins, providing strong bases for the unambiguous resonance assignments and information concerning the structure, dynamics, and intermolecular interactions. Amino acid selective 15N labeling suffers fro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00182

    authors： Sugiki T,Furuita K,Fujiwara T,Kojima C

    更新日期：2018-07-03 00:00:00

  • Solution conformation of asparagine-linked oligosaccharides: alpha(1-2)-, alpha(1-3)-, beta(1-2)-, and beta(1-4)-linked units.

    abstract：:The solution conformation is presented for representatives of each of the major classes of asparaginyl oligosaccharides. In this report the conformation of alpha(1-3)-, alpha(1-2)-, beta(1-2)-, and beta(1-4)-linked units is described. The conformational properties of these glycopeptides were determined by high-resolut...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00284a021

    authors： Brisson JR,Carver JP

    更新日期：1983-07-19 00:00:00

  • Formation of a bis(histidyl) heme iron complex in manganese peroxidase at high pH and restoration of the native enzyme structure by calcium.

    abstract：:Manganese peroxidase (MnP) from Phanerochaete chrysosporium undergoes a pH-dependent conformational change evidenced by changes in the electronic absorption spectrum. This high- to low-spin alkaline transition occurs at approximately 2 pH units lower in an F190I mutant MnP when compared to the wild-type enzyme. Herein...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000679j

    authors： Youngs HL,Moënne-Loccoz P,Loehr TM,Gold MH

    更新日期：2000-08-15 00:00:00

  • Involvement of serine 96 in the catalytic mechanism of ferredoxin-NADP+ reductase: structure--function relationship as studied by site-directed mutagenesis and X-ray crystallography.

    abstract：:The crystal structure of ferredoxin-NADP+ reductase (FNR) suggests that Ser96 is directly involved in hydride transfer between the isoalloxazine moiety of FAD and the nicotinamide ring of NADP(H). To probe its role, Ser96 has been mutated to valine (S96V) and glycine (S96G). These mutations primarily affected the inte...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00026a019

    authors： Aliverti A,Bruns CM,Pandini VE,Karplus PA,Vanoni MA,Curti B,Zanetti G

    更新日期：1995-07-04 00:00:00

  • Structural and functional modulation of the manganese cluster in Ca(2+)-depleted photosystem II induced by binding of the 24-kilodalton extrinsic protein.

    abstract：:Depletion of functional Ca2+ from photosystem (PS) II membranes impairs O2 evolution. Redox properties of the Mn cluster as probed by thermoluminescence were modified differently in Ca(2+)-depleted PSII depending on the procedure for Ca2+ extraction. Ca2+ depletion by low-pH treatment gave rise to an abnormally modifi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00148a028

    authors： Ono T,Izawa S,Inoue Y

    更新日期：1992-08-25 00:00:00

  • Two-step purification of the major phosphorylated protein in reticulocyte 40S ribosomal subunits.

    abstract：:In reticulocytes, a single ribosomal protein, S13, has been shown to be phosphorylated by the cAMP-regulated protein kinases. The 40S ribosomal subunits were phosphorylated in vitro with [gamma-32P]ATP to facilitate the identification of S13 during the two-step purification procedure. Total ribosomal protein from the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00604a003

    authors： Du Vernay VH Jr,Traugh JA

    更新日期：1978-05-30 00:00:00

  • Determination of the three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor from ragi seeds by NMR spectroscopy.

    abstract：:The three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor (RBI) from seeds of ragi (Eleusine coracana Gaertneri) has been determined in solution using multidimensional 1H and 15N NMR spectroscopy. The inhibitor consists of 122 amino acids, with 5 disulfide bridges, and belongs to the plant al...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00026a009

    authors： Strobl S,Mühlhahn P,Bernstein R,Wiltscheck R,Maskos K,Wunderlich M,Huber R,Glockshuber R,Holak TA

    更新日期：1995-07-04 00:00:00

  • Picosecond tryptophan fluorescence of thioredoxin: evidence for discrete species in slow exchange.

    abstract：:The steady-state tryptophan fluorescence and time-resolved tryptophan fluorescence of Escherichia coli thioredoxin, calf thymus thioredoxin, and yeast thioredoxin have been studied. In all proteins, the tryptophan residues undergo strong static and dynamic quenching, probably due to charge-transfer interactions with t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00434a038

    authors： Mérola F,Rigler R,Holmgren A,Brochon JC

    更新日期：1989-04-18 00:00:00

  • Photooxidation pathway of chlorophyll Z in photosystem II as Studied by Fourier transform infrared spectroscopy.

    abstract：:The oxidation pathway of chlorophyll Z (ChlZ) in photosystem II (PSII) at cryogenic temperatures was studied by means of light-induced Fourier transform infrared (FTIR) difference spectroscopy. To examine the involvement of redox-active beta-carotene (Car) in the pathway, two Car molecules in Mn-depleted PSII membrane...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052346y

    authors： Kitajima Y,Noguchi T

    更新日期：2006-02-14 00:00:00

  • Enzyme-catalyzed condensation reactions which initiate rapid peptic cleavage of substrates. 1. How the structure of an activating peptide determines its efficiency.

    abstract：:The addition of a small peptide can significantly increase the rate at which pepsin cleaves a substrate at pH 4.5. Why? In order to find out, we have determined spectrophotometrically the relative ability of over a dozen peptides to speed the initial rate of disappearance of Phe-Trp-NH2 and Leu-Trp-Met-Arg. Here are s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00514a029

    authors： Silver MS,James SL

    更新日期：1981-05-26 00:00:00

  • The C-terminal aqueous-exposed domain of the 45 kDa subunit of the particulate methane monooxygenase in Methylococcus capsulatus (Bath) is a Cu(I) sponge.

    abstract：:The crystal structure of the particulate methane monooxygenase (pMMO) from Methylococcus capsulatus (Bath) has been reported recently [Lieberman, R. L., and Rosenzweig, A. C. (2005) Crystal structure of a membrane-bound metalloenzyme that catalyses the biological oxidation of methane, Nature 434, 177-182]. Subsequent ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700883g

    authors： Yu SS,Ji CZ,Wu YP,Lee TL,Lai CH,Lin SC,Yang ZL,Wang VC,Chen KH,Chan SI

    更新日期：2007-12-04 00:00:00

  • Atomistic Characterization of the First Step of Calcium Pump Activation Associated with Proton Countertransport.

    abstract：:The calcium pump [sarcoplasmic reticulum (SR) Ca(2+)-ATPase (SERCA)] transports Ca(2+) from the cytosol to the SR lumen at the expense of ATP hydrolysis and proton countertransport, thus playing a central role in Ca(2+) homeostasis and muscle contractility. Proton countertransport via deprotonation of transport site r...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00672

    authors： Ramírez-Salinas GL,Espinoza-Fonseca LM

    更新日期：2015-08-25 00:00:00

  • Characterization of a cloned human dihydrotestosterone/androstanediol UDP-glucuronosyltransferase and its comparison to other steroid isoforms.

    abstract：:A human cDNA, UDPGTh-3, encoding a dihydrotestosterone/5 alpha-androstane-3 alpha,17 beta-diol UDP- glucuronosyltransferase (transferase) has been isolated and characterized. The nucleotide sequence of UDPGTh-3 encodes a 530 amino acid protein with a typical membrane insertion-signal peptide, a membrane-anchoring doma...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00091a015

    authors： Chen F,Ritter JK,Wang MG,McBride OW,Lubet RA,Owens IS

    更新日期：1993-10-12 00:00:00

  • Novel small molecules relieve prothymosin alpha-mediated inhibition of apoptosome formation by blocking its interaction with Apaf-1.

    abstract：:Structurally diverse small molecules, including 5-(2-benzofuryl)-4-phenyl-1,2,4-triazole-3-thiol (BETT), have been identified via high-throughput screening as activators of caspase-3 in HeLa cell extracts. However, little is known about their mechanism of action. In this study, we investigate how BETT regulates prothy...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9022329

    authors： Qi X,Wang L,Du F

    更新日期：2010-03-09 00:00:00

  • NH2-terminal extensions on skin collagen from sheep with a genetic defect in conversion of procollagen into collagen.

    abstract：:A modified form of procollagen was extracted with 10 M urea from the skin of lambs with dermatosparaxis, a disease which is produced by a genetic defect in the conversion of procollagen to collagen. The extracts contained little if any alpha1 and alpha2 chains of normal type I collagen, and instead they contained the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00658a024

    authors： Becker U,Timpl R

    更新日期：1976-06-29 00:00:00

  • Phospholipids stabilize the interaction between the alpha and beta subunits of the solubilized receptor for immunoglobulin E.

    abstract：:The cell-surface component (alpha) which binds monomeric immunoglobulin E with high affinity is associated with a second polypeptide (beta) in the plasma membrane. The latter component tends to dissociate during purification of the alpha chain from detergent extracts of cells, even at neutral pHs and physiological ion...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00269a047

    authors： Rivnay B,Wank SA,Poy G,Metzger H

    更新日期：1982-12-21 00:00:00

  • Constructing de novo biosynthetic pathways for chemical synthesis inside living cells.

    abstract：:Living organisms have evolved a vast array of catalytic functions that make them ideally suited for the production of medicinally and industrially relevant small-molecule targets. Indeed, native metabolic pathways in microbial hosts have long been exploited and optimized for the scalable production of both fine and co...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi200416g

    authors： Weeks AM,Chang MC

    更新日期：2011-06-21 00:00:00

  • Structural and spectroscopic studies of the copper site of stellacyanin.

    abstract：:The structure of the copper site in oxidized and reduced Rhus vernicifera stellacyanin has been studied by X-ray absorption (XAFS) spectroscopy at different pH values. Data for the oxidized protein are consistent with the fourth ligand being an O- or N-donating ligand rather than a cysteine from the disulfide bridge. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00001a026

    authors： Strange RW,Reinhammar B,Murphy LM,Hasnain SS

    更新日期：1995-01-10 00:00:00

  • Binding of calcium to anticoagulant protein S: role of the fourth EGF module.

    abstract：:Protein S is an anticoagulant protein containing a Gla (enclosing gamma-carboxyglutamic acids) module, a TSR (thrombin sensitive region) module, four EGF (epidermal growth factor)-like modules, and a SHBG (sex hormone binding globulin)-like region. Protein S is a cofactor to activated protein C (APC) in the degradatio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0601151

    authors： Persson KE,Stenflo J,Linse S,Stenberg Y,Preston RJ,Lane DA,Rezende SM

    更新日期：2006-09-05 00:00:00

  • Phosphorylation of the lysine-rich histones throughout the cell cycle.

    abstract：:The phosphorylating of the lysine-rich histone at various stages in the cell cycle has been studied. In rapidly dividing cell populations the lysine-rich histone is phosphorylated rapidly after synthesis and more slowly once bound to the chromosome. The half-life of hydrolysis of such interphase phosphorylation in 5 h...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00682a033

    authors： Balhorn R,Jackson V,Granner D,Chalkey R

    更新日期：1975-06-03 00:00:00

  • Engineering the zinc binding site of human carbonic anhydrase II: structure of the His-94-->Cys apoenzyme in a new crystalline form.

    abstract：:The structure of the His-94-->Cys variant of human carbonic anhydrase II (CAII) has been determined by X-ray crystallographic methods to a resolution of 2.3 A with a final crystallographic R factor of 0.155. This variant of CAII crystallizes in orthorhombic space group P2(1)2(1)2(1) which is the first example of a new...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00057a015

    authors： Alexander RS,Kiefer LL,Fierke CA,Christianson DW

    更新日期：1993-02-16 00:00:00

  • Denaturation of cytochrome P450 2B1 by guanidine hydrochloride and urea: evidence for a metastable intermediate state of the active site.

    abstract：:A metastable intermediate was found in the course of the denaturation of purified cytochrome P450 2B1 by increasing concentrations of guanidine hydrochloride (GuHCl). The metastable intermediate has no or low absorbance at 450 nm in the reduced carbon monoxide difference spectrum and has no absorbance at 420 nm. The i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00016a023

    authors： Yu XC,Shen S,Strobel HW

    更新日期：1995-04-25 00:00:00

  • Hydration of dA.dT polymers: role of water in the thermodynamics of ethidium and propidium intercalation.

    abstract：:We report differences in the interaction of two structurally similar phenanthroline intercalators, ethidium and propidium, with poly(dA).poly(dT) and poly[d(A-T)] as a function of ionic strength based on titration microcalorimetry, fluorescence titration, and hydrostatic pressure measurements. Both ethidium and propid...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00472a009

    authors： Marky LA,Macgregor RB Jr

    更新日期：1990-05-22 00:00:00

  • Independent functions for the N- and C-termini in the overlap region of tropomyosin.

    abstract：:Tropomyosin (TM) is a coiled-coil that binds head-to-tail along the helical actin filament. The ends of 284-residue tropomyosins are believed to overlap by about nine amino acids. The present study investigates the function of the N- and C-terminal overlap regions. Recombinant tropomyosins were produced in Escherichia...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000242b

    authors： Moraczewska J,Hitchcock-DeGregori SE

    更新日期：2000-06-13 00:00:00

  • Detergents stabilize the conformation of phosphodiesterase 6.

    abstract：:Membrane-bound phosphodiesterase 6 (PDE6) plays an important role in visual signal transduction by regulating cGMP levels in rod photoreceptor cells. Our understanding of PDE6 catalysis and structure suffers from inadequate characterization of the α and β subunit catalytic core, interactions of the core with two intri...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi2014695

    authors： Baker BY,Palczewski K

    更新日期：2011-11-08 00:00:00