Phospholipids stabilize the interaction between the alpha and beta subunits of the solubilized receptor for immunoglobulin E.

abstract：:A comparison of the oxidations of diclofenac with microsomes of yeasts expressing various human liver cytochromes P450 showed that P450 2C9 regioselectively led to 4'-hydroxy diclofenac (4'-OHD) whereas P450 3A4 only led to 5-hydroxy diclofenac (5-OHD). P450 2C19, 2C18, and 2C8 led to the simultaneous formation of 4'-...

journal_title：Biochemistry

authors： Mancy A,Antignac M,Minoletti C,Dijols S,Mouries V,Duong NT,Battioni P,Dansette PM,Mansuy D

更新日期：1999-10-26 00:00:00

abstract：:A wide range of two-dimensional 1H NMR experiments have been used to completely assign the 500-MHz 1H NMR spectrum of recombinant Ca2+-saturated bovine calbindin D9k (76 amino acids, Mr = 8500). In solution, calbindin D9k exists as an equilibrium mixture of isoforms with trans (75%) and cis (25%) isomers of the peptid...

journal_title：Biochemistry

authors： Kördel J,Forsén S,Chazin WJ

更新日期：1989-08-22 00:00:00

abstract：:Synthetic O-glycopeptides containing one or two GalNAc residues attached to Ser or Thr were used as substrates to investigate the effect of peptide structure on the activity of crude preparations of UDP-Gal:GalNAc alpha-R beta 3-Gal-transferase from pig stomach and pig and rat colonic mucosa and of a partially purifie...

journal_title：Biochemistry

authors： Brockhausen I,Möller G,Merz G,Adermann K,Paulsen H

更新日期：1990-11-06 00:00:00

abstract：:Comparison of available sequences of HLA-A and HLA-B antigens shows that variable positions are predominantly localized in four segments spanning residues 63-85, 105-116, 138-156, and 177-194. The fourth segment is unique in that it contains no differences between antigens of the same locus. Secondary folding of HLA h...

journal_title：Biochemistry

authors： Vega MA,Bragado R,Ezquerra A,López de Castro JA

更新日期：1984-02-28 00:00:00

abstract：:Transcarboxylase is made up of a central hexameric subunit (S20,W similar 12 S), three peripheral dimeric metallo subunits (S20,W similar to 5 S), and six biotinyl carboxyl carrier subunits (S20,W similar to 1.3 S). The results presented here show that the carboxyl carrier subunit is required for assembly of the 12S a...

journal_title：Biochemistry

authors： Ahmad F,Jacobson B,Chuang M,Brattin W,Wood HG

更新日期：1975-04-22 00:00:00

abstract：:The interaction of bleomycin A2 with DNA has been examined by fluorescence spectroscopy and proton magnetic resonance techniques. Fluorescence bands observed at 353 and 405 nm in the spectrum of bleomycin were assigned to the bithiazole and 4-aminopyrimidine rings, respectively. Quenching of bithiazole fluorescence by...

journal_title：Biochemistry

authors： Chien M,Grollman AP,Horwitz SB

更新日期：1977-08-09 00:00:00

abstract：:Bacterial NO synthase (NOS)-like proteins such as that from Bacillus subtilis (bsNOS) share a high degree of structural homology with the oxygenase domain of mammalian NOSs (mNOSs), but biochemical studies have yet failed to establish that they are specifically capable of producing NO. To better understand the actual ...

journal_title：Biochemistry

authors： Santolini J,Roman M,Stuehr DJ,Mattioli TA

更新日期：2006-02-07 00:00:00

abstract：:HPr is a protein of the bacterial phosphoenolpyruvate:sugar phosphotransferase transport system (PTS). In Gram-positive bacteria, HPr can be phosphorylated on Ser(46) by HPr(Ser) kinase/phosphorylase (HPrK/P) and on His(15) by enzyme I (EI) of the PTS. In vitro studies have shown that phosphorylation on one residue gr...

journal_title：Biochemistry

authors： Casabon I,Couture M,Vaillancourt K,Vadeboncoeur C

更新日期：2006-05-30 00:00:00

abstract：:The effect of substituting Pro-L209 with Tyr, Phe, Glu, and Thr in photosynthetic reaction centers (RCs) from Rhodobacter sphaeroides was investigated by monitoring the light-induced FTIR absorption changes associated with the photoreduction of the secondary quinone Q(B). Pro-L209 is close to a chain of ordered water ...

journal_title：Biochemistry

authors： Nabedryk E,Breton J,Sebban P,Baciou L

更新日期：2003-05-20 00:00:00

abstract：:Escherichia coli, Clostridium sticklandii, and Methanococcus vannielii synthesize 75Se-labeled amino acid transfer ribonucleic acids [( 75Se]tRNAs) when grown with low levels (approximately equal to 1 microM) of 75SeO32-. When E. coli [75Se]tRNA was digested to nucleosides and analyzed by reversed-phase high-performan...

journal_title：Biochemistry

authors： Wittwer AJ,Tsai L,Ching WM,Stadtman TC

更新日期：1984-09-25 00:00:00

abstract：:The sensor kinase CitA and the response regulator CitB of Klebsiella pneumoniae form the paradigm of a subfamily of bacterial two-component regulatory systems that are capable of sensing tri- or dicarboxylates in the environment and then induce transporters for the uptake of these compounds. We recently showed that th...

journal_title：Biochemistry

authors： Gerharz T,Reinelt S,Kaspar S,Scapozza L,Bott M

更新日期：2003-05-20 00:00:00

abstract：:We report nuclear magnetic resonance (NMR) data indicating that the Rieske protein from the cytochrome bc complex of Thermus thermophilus (TtRp) undergoes modest redox-state-dependent and ligand-dependent conformational changes. To test models concerning the mechanism by which TtRp transfers between different sites on...

journal_title：Biochemistry

authors： Hsueh KL,Tonelli M,Cai K,Westler WM,Markley JL

更新日期：2013-04-30 00:00:00

abstract：:A 16-mer deoxyribonucleotide was labeled at the 5'-end with x-rhodamine and at the 3'-end with fluorescein. Förster resonance energy transfer was used to determine the distribution, P(R), of donor-acceptor distances for the oligomer in three duplex structures (hybridized to complementary strands having 10, 16, and 24 ...

journal_title：Biochemistry

authors： Parkhurst KM,Parkhurst LJ

更新日期：1995-01-10 00:00:00

abstract：:Catalase (hydroperoxidase) HPII of Escherichia coli is the largest catalase so far characterized, existing as a homotetramer of 84 kDa subunits. Each subunit has a core structure that closely resembles small subunit catalases, supplemented with an extended N-terminal sequence and compact flavodoxin-like C-terminal dom...

journal_title：Biochemistry

authors： Chelikani P,Donald LJ,Duckworth HW,Loewen PC

更新日期：2003-05-20 00:00:00

abstract：:We describe an assay for detecting intramolecular triple-stranded DNA in living cells. The assay involves quantitative analysis of the differential photobinding of 4,5',8-trimethylpsoralen to 5'TA and 5'AT dinucleotides in a region of plasmid DNA containing the triplex-forming sequence (GA)7TA(GA)7. Psoralen photobind...

journal_title：Biochemistry

authors： Ussery DW,Sinden RR

更新日期：1993-06-22 00:00:00

abstract：:The water-oxidizing complex of photosystem II cycles through five oxidation states, denoted S(i)() (i = 0-4), during water oxidation to molecular oxygen, which appears at the (transient) S(4) state. The recent detection of bimodal EPR signals from the S(3) state [Matsukawa, T., Mino, H., Yoneda, D., Kawamori, A. (1999...

journal_title：Biochemistry

authors： Ioannidis N,Petrouleas V

更新日期：2002-07-30 00:00:00

abstract：:Weak or nonexistent smallpox immunity in today's human population raises concerns about the possibility of natural or provoked genetic modifications leading to re-emergence of variola virus and other poxviruses. Thus, the development of new antiviral strategies aimed at poxvirus infections in humans is a high priority...

journal_title：Biochemistry

authors： Duraffour S,Ishchenko AA,Saparbaev M,Crance JM,Garin D

更新日期：2007-10-23 00:00:00

abstract：:Our previous studies revealed that interaction of fibrin with the very low density lipoprotein (VLDL) receptor plays a prominent role in transendothelial migration of leukocytes and thereby inflammation. The major goal of our subsequent studies is to establish the structural basis for this interaction. As the first st...

journal_title：Biochemistry

authors： Banerjee K,Yakovlev S,Gruschus JM,Medved L,Tjandra N

更新日期：2018-07-31 00:00:00

abstract：:Blastochloris tepida is a newly described thermophilic purple bacterium containing bacteriochlorophyll b. Using purified light-harvesting 1 reaction center (LH1-RC) core complexes from Blc. tepida, we compared the biochemical, spectroscopic, and thermal denaturation properties of these complexes with those of its meso...

journal_title：Biochemistry

authors： Seto R,Takaichi S,Kurihara T,Kishi R,Honda M,Takenaka S,Tsukatani Y,Madigan MT,Wang-Otomo ZY,Kimura Y

更新日期：2020-06-30 00:00:00

abstract：:Fibroblast growth factors (FGFs) are mitogenic and chemotactic agents for a wide variety of cell types and play a primary role in the regulation of angiogenesis. Angiogenesis is involved in a variety of critical physiological events including organogenesis, wound healing, ischemic collateral circulation, and solid tum...

journal_title：Biochemistry

authors： Blaber M,DiSalvo J,Thomas KA

更新日期：1996-02-20 00:00:00

abstract：:Extracellular matrix (ECM) proteins, such as collagen and fibronectin, play vital roles in development and maintenance of hard tissue (bone or tooth) and are, consequently, thoroughly investigated for construction of biomimetic scaffolds in combination with calcium phosphate (CaP) material (the major component of hard...

journal_title：Biochemistry

authors： Chowdhury EH,Nagaoka M,Ogiwara K,Zohra FT,Kutsuzawa K,Tada S,Kitamura C,Akaike T

更新日期：2005-09-20 00:00:00

abstract：:The structure and dynamic properties of the C-terminal region of the human class alpha glutathione transferase A1-1 have been investigated with high-resolution NMR methods. On the basis of crystallographic and fluorescence measurements, this 13-residue segment of the enzyme is presumed to be disordered in the unligand...

journal_title：Biochemistry

authors： Zhan Y,Rule GS

更新日期：2004-06-15 00:00:00

abstract：:A dimeric bis-benzimidazole molecule has been designed by computer modeling to bind to a DNA sequence via the DNA minor groove that covers a complete turn of B-DNA. A series of bis-benzimidazole dimers incorporating a -O-(CH(2))(n)()-X-CH(2))(n)()-O- linker, with n = 2 or 3 and X = O or N(+)H(Me), were screened for th...

journal_title：Biochemistry

authors： Joubert A,Sun XW,Johansson E,Bailly C,Mann J,Neidle S

更新日期：2003-05-27 00:00:00

abstract：:Chorismate-utilizing enzymes catalyze diverse reactions, providing critical physiological functions unique to plants, bacteria, fungi, and some parasites. Their absence in animals makes them excellent targets for antimicrobials and herbicides. 4-Amino-4-deoxychorismate synthase (ADCS) catalyzes the first step in folat...

journal_title：Biochemistry

authors： He Z,Toney MD

更新日期：2006-04-18 00:00:00

abstract：:The local environment of the transmembrane and C-terminal domain of M13 major coat protein was probed by site-directed ESR spin labeling when the protein was introduced into three membrane-mimicking systems, DOPC vesicles, sodium cholate micelles, and SDS micelles. For this purpose, we have inserted unique cysteine re...

journal_title：Biochemistry

authors： Stopar D,Spruijt RB,Wolfs CJ,Hemminga MA

更新日期：1996-12-03 00:00:00

abstract：:Synthesis of a novel perylene-neomycin conjugate (3) and the properties of its binding to human telomeric G-quadruplex DNA, 5'-d[AG3(T2AG3)3] (4), are reported. Various spectroscopic techniques were employed to characterize the binding of conjugate 3 to 4. A competition dialysis assay revealed that 3 preferentially bi...

journal_title：Biochemistry

authors： Xue L,Ranjan N,Arya DP

更新日期：2011-04-12 00:00:00

abstract：:Apolipoproteins A-I and A-II form the major protein constituents of high-density lipid particles (HDL), the concentration of which is inversely correlated with the frequency of heart disease in humans. Although the physiological role of apolipoprotein A-II is unclear, evidence for its involvement in free fatty acid me...

journal_title：Biochemistry

authors： Kumar MS,Carson M,Hussain MM,Murthy HM

更新日期：2002-10-01 00:00:00

abstract：:Rhodopsin is the photoreceptor in vertebrate rod cells responsible for vision at low light intensities. The photoreactive chromophore in rhodopsin is 11-cis-retinal bound to the protein via a protonated Schiff base with Glu113 as the counterion. We have used the observed 13C NMR chemical shifts of the conjugated retin...

journal_title：Biochemistry

authors： Han M,Smith SO

更新日期：1995-01-31 00:00:00

abstract：:Cyanobactin heterocyclases share the same catalytic domain (YcaO) as heterocyclases/cyclodehydratases from other ribosomal peptide (RiPPs) biosynthetic pathways. These enzymes process multiple residues (Cys/Thr/Ser) within the same substrate. The processing of cysteine residues proceeds with a known order. We show the...

journal_title：Biochemistry

authors： Ge Y,Czekster CM,Miller OK,Botting CH,Schwarz-Linek U,Naismith JH

更新日期：2019-04-23 00:00:00

abstract：:With respect to the functional importance attributed to the N-terminal part of the Na(+)/proline transporter of Escherichia coli (PutP), we report here on the structural arrangement and functional dynamics of transmembrane domains (TMs) II and III and the adjoining loop regions. Information on membrane topography was ...

journal_title：Biochemistry

authors： Wegener C,Tebbe S,Steinhoff HJ,Jung H

更新日期：2000-04-25 00:00:00