当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Structures of apolipoprotein A-II and a lipid-surrogate complex provide insights into apolipoprotein-lipid interactions.

Structures of apolipoprotein A-II and a lipid-surrogate complex provide insights into apolipoprotein-lipid interactions.

Abstract：

:Apolipoproteins A-I and A-II form the major protein constituents of high-density lipid particles (HDL), the concentration of which is inversely correlated with the frequency of heart disease in humans. Although the physiological role of apolipoprotein A-II is unclear, evidence for its involvement in free fatty acid metabolism in mice has recently been obtained. Currently, the best characterized activity of apolipoprotein A-II is its potent antagonism of the anti-atherogenic and anti-inflammatory activities of apolipoprotein A-I, probably due to its competition with the latter for lipid acyl side chains in HDL. Many interactions of apolipoprotein A-I with enzymes and proteins involved in reverse cholesterol transport and HDL maturation are mediated by lipid-bound protein. The structural bases of interaction with lipids are expected to be common to exchangeable apolipoproteins and attributable to amphipathic alpha-helices present in each of them. Thus, characterization of apolipoprotein-lipid interactions in any apolipoprotein is likely to provide information that is applicable to the entire class. We report structures of human apolipoprotein A-II and its complex with beta-octyl glucoside, a widely used lipid surrogate. The former shows that disulfide-linked dimers of apolipoprotein A-II form amphipathic alpha-helices which aggregate into tetramers. Dramatic changes, observed in the presence of beta-octyl glucoside, might provide clues to the structural basis for its antagonism of apolipoprotein A-I. Additionally, excursions of individual molecules of apolipoprotein A-II from a common helical architecture in both structures indicate that lipid-bound apolipoproteins are likely to have an ensemble of related conformations. These structures provide the first experimental paradigm for description of apolipoprotein-lipid interactions at the atomic level.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Kumar MS,Carson M,Hussain MM,Murthy HM

doi

10.1021/bi026069w

subject

Has Abstract

pub_date

2002-10-01 00:00:00

pages

11681-91

issue

39

eissn

0006-2960

issn

1520-4995

pii

bi026069w

journal_volume

41

pub_type

杂志文章,收录出版

相关文献

BIOCHEMISTRY文献大全
  • Parameters affecting low-pH-mediated fusion of liposomes with the plasma membrane of cells infected with influenza virus.

    abstract：:Unilamellar liposomes can be fused at low pH with the plasma membrane of cells that express the hemagglutinin glycoprotein of influenza virus on their surface [van Meer, G., & Simons, K. (1983) J. Cell Biol. 97, 1365-1374]. Here, we have resolved this fusion process into two kinetically distinct steps. The first and m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00335a030

    authors： van Meer G,Davoust J,Simons K

    更新日期：1985-07-02 00:00:00

  • Dynamics, energetics, and structure in protein folding.

    abstract：:For many decades, protein folding experimentalists have worked with no information about the time scales of relevant protein folding motions and without methods for estimating the height of folding barriers. Protein folding experiments have been interpreted using chemical models in which the folding process is charact...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi060643c

    authors： Naganathan AN,Doshi U,Fung A,Sadqi M,Muñoz V

    更新日期：2006-07-18 00:00:00

  • Probing the interactions of alcohols with biological membranes with the fluorescent probe Prodan.

    abstract：:Prodan [6-propionyl-2-(dimethylamine)naphthalene] is a hydrophobic fluorescent probe which is extremely sensitive to both the polarity and the hydrogen-bond donating capacity of the solvent. In binary mixtures of solvents, the hydrogen-bond donating effect on Prodan fluorescence saturates at relatively low concentrati...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00154a021

    authors： Rottenberg H

    更新日期：1992-10-06 00:00:00

  • Thymidylate synthetase catalyzed exchange of tritiumfrom [5-3H]-2'-deoxyuridylate for protons of water.

    abstract：:Thymidylate synthetase catalyzes an exchange of tritium of [5-3H]dUMP for protons of water in the absence of CH2-H4folate. The turnover number for this reaction is some 45,000-fold lower than that of dTMP formation and Km is 1.2 X 10(-5) M, similar to the dissociation constant of the enzyme-dUMP complex determined by ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00580a016

    authors： Pogolotti AL Jr,Weill C,Santi DV

    更新日期：1979-06-26 00:00:00

  • Influence of aggregation propensity and stability on amyloid fibril formation as studied by Fourier transform infrared spectroscopy and two-dimensional COS analysis.

    abstract：:Understanding the process of amyloidogenesis is important for the future treatment of misfolding-based diseases, such as Alzheimer's, spongiform encephalopathies, and other important disorders affecting humans. In this work, we have used one of the best-characterized models for folding and misfolding, the activation d...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900960s

    authors： Cerdà-Costa N,De la Arada I,Avilés FX,Arrondo JL,Villegas S

    更新日期：2009-11-10 00:00:00

  • Single-turnover and pre-steady-state kinetics of the reaction of the adenine glycosylase MutY with mismatch-containing DNA substrates.

    abstract：:The DNA repair enzyme MutY plays an important role in the prevention of DNA mutations resulting from the presence of the oxidatively damaged lesion 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG) in DNA by the removal of misincorporated adenine residues in OG:A mispairs. MutY also exhibits adenine glycosylase activity toward...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981594+

    authors： Porello SL,Leyes AE,David SS

    更新日期：1998-10-20 00:00:00

  • Identification of a new class of inhibitors of the voltage-gated potassium channel, Kv1.3, with immunosuppressant properties.

    abstract：:The voltage-gated potassium channel, K(v)1.3, is a novel target for development of immunosuppressants. Using a functional (86)Rb(+) efflux assay, a new class of high-affinity K(v)1.3 inhibitors has been identified. The initial active in this series, 4-phenyl-4-[3-(2-methoxyphenyl)-3-oxo-2-azaprop-1-yl]cyclohexanone (P...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi025722c

    authors： Schmalhofer WA,Bao J,McManus OB,Green B,Matyskiela M,Wunderler D,Bugianesi RM,Felix JP,Hanner M,Linde-Arias AR,Ponte CG,Velasco L,Koo G,Staruch MJ,Miao S,Parsons WH,Rupprecht K,Slaughter RS,Kaczorowski GJ,Garcia ML

    更新日期：2002-06-18 00:00:00

  • Replication, repair, and translesion polymerase bypass of N⁶-oxopropenyl-2'-deoxyadenosine.

    abstract：:The oxidative stress products malondialdehyde and base propenal react with DNA bases forming the adduction products 3-(2'-deoxy-β-D-erythro-pentofuranosyl)pyrimido[1,2-a]purin-10(3H)-one (M1dG) and N(6)-(oxypropenyl)-2'-deoxyadenosine (OPdA). M1dG is mutagenic in vivo and miscodes in vitro, but little work has been do...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi401103k

    authors： Maddukuri L,Shuck SC,Eoff RL,Zhao L,Rizzo CJ,Guengerich FP,Marnett LJ

    更新日期：2013-12-03 00:00:00

  • A hexameric helicase encircles one DNA strand and excludes the other during DNA unwinding.

    abstract：:The bacteriophage T7 DNA helicase/primase (gene 4 protein) is a ring-like hexamer that encircles ssDNA and requires forked DNA to catalyze DNA unwinding. We report that optimal rates of unwinding of forked DNA require ssDNA tails of 55 nucleotides on the 5'-to-3' strand and 15 nucleotides on the 3'-to-5' strand. Surpr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971644v

    authors： Hacker KJ,Johnson KA

    更新日期：1997-11-18 00:00:00

  • Effects of adenosine 5'-diphosphate on bovine glutamate dehydrogenase: diethyl pyrocarbonate modification.

    abstract：:Initial rate kinetic studies and reduced coenzyme binding studies with bovine glutamate dehydrogenase have shown that an enzyme-NAD(P)H-glutamate abortive complex is a major participant in the overall enzyme-catalyzed reaction. The allosteric regulator ADP is shown to activate the enzyme by destabilizing this abortive...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00567a017

    authors： George A,Bell JE

    更新日期：1980-12-23 00:00:00

  • Conservative tryptophan mutants of the protein tyrosine phosphatase YopH exhibit impaired WPD-loop function and crystallize with divanadate esters in their active sites.

    abstract：:Catalysis in protein tyrosine phosphatases (PTPs) involves movement of a protein loop called the WPD loop that brings a conserved aspartic acid into the active site to function as a general acid. Mutation of the tryptophan in the WPD loop of the PTP YopH to any other residue with a planar, aromatic side chain (phenyla...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00496

    authors： Moise G,Gallup NM,Alexandrova AN,Hengge AC,Johnson SJ

    更新日期：2015-10-27 00:00:00

  • Activity screening of carrier domains within nonribosomal peptide synthetases using complex substrate mixtures and large molecule mass spectrometry.

    abstract：:For screening a pool of potential substrates that load carrier domains found in nonribosomal peptide synthetases, large molecule mass spectrometry is shown to be a new, unbiased assay. Combining the high resolving power of Fourier transform mass spectrometry with the ability of adenylation domains to select their own ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052333k

    authors： Dorrestein PC,Blackhall J,Straight PD,Fischbach MA,Garneau-Tsodikova S,Edwards DJ,McLaughlin S,Lin M,Gerwick WH,Kolter R,Walsh CT,Kelleher NL

    更新日期：2006-02-14 00:00:00

  • Structural and dynamic study of the tetramerization region of non-erythroid alpha-spectrin: a frayed helix revealed by site-directed spin labeling electron paramagnetic resonance.

    abstract：:The N-terminal region of alpha-spectrin is responsible for its association with beta-spectrin in a heterodimer, forming functional tetramers. Non-erythroid alpha-spectrin (alphaII-spectrin) has a significantly higher association affinity for beta-spectrin than the homologous erythroid alpha-spectrin (alphaI-spectrin)....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8013032

    authors： Li Q,Fung LW

    更新日期：2009-01-13 00:00:00

  • Tyrosine kinase-mediated serine phosphorylation of adenylyl cyclase.

    abstract：:Receptor tyrosine kinase (RTK) activation is associated with modulation of heptahelical receptor-stimulated adenylyl cyclase responses. The mechanisms underlying the RTK-mediated enhancement of adenylyl cyclase function remain unclear. In the present studies, we show that the tyrosine kinase-dependent enhancement of a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0015818

    authors： Tan CM,Kelvin DJ,Litchfield DW,Ferguson SS,Feldman RD

    更新日期：2001-02-13 00:00:00

  • Substrate-Induced Facilitated Dissociation of the Competitive Inhibitor from the Active Site of O-Acetyl Serine Sulfhydrylase Reveals a Competitive-Allostery Mechanism.

    abstract：:By classical competitive antagonism, a substrate and competitive inhibitor must bind mutually exclusively to the active site. The competitive inhibition of O-acetyl serine sulfhydrylase (OASS) by the C-terminus of serine acetyltransferase (SAT) presents a paradox, because the C-terminus of SAT binds to the active site...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00500

    authors： Singh AK,Ekka MK,Kaushik A,Pandya V,Singh RP,Banerjee S,Mittal M,Singh V,Kumaran S

    更新日期：2017-09-19 00:00:00

  • Crystal structures of two self-hydroxylating ribonucleotide reductase protein R2 mutants: structural basis for the oxygen-insertion step of hydroxylation reactions catalyzed by diiron proteins.

    abstract：:The R2 protein of ribonucleotide reductase catalyzes the dioxygen-dependent one-electron oxidation of Tyr122 at a diiron-carboxylate site. Methane monooxygenase and related hydroxylases catalyze hydrocarbon hydroxylation at diiron sites structurally related to the one in R2. In protein R2, the likely reaction site for...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9806403

    authors： Logan DT,deMaré F,Persson BO,Slaby A,Sjöberg BM,Nordlund P

    更新日期：1998-07-28 00:00:00

  • Fungal heme oxygenases: Functional expression and characterization of Hmx1 from Saccharomyces cerevisiae and CaHmx1 from Candida albicans.

    abstract：:Heme oxygenases convert heme to free iron, CO, and biliverdin. Saccharomyces cerevisiae and Candida albicans express putative heme oxygenases that are required for the acquisition of iron from heme, a critical process for fungal survival and virulence. The putative heme oxygenases Hmx1 and CaHmx1 from S. cerevisiae an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061429r

    authors： Kim D,Yukl ET,Moënne-Loccoz P,Montellano PR

    更新日期：2006-12-12 00:00:00

  • Differential cytotoxicity of human wild type and mutant alpha-synuclein in human neuroblastoma SH-SY5Y cells in the presence of dopamine.

    abstract：:Parkinson's disease (PD) involves loss of dopaminergic neurons in the substantia nigra and is characterized by intracellular inclusions, Lewy bodies, consisting primarily of aggregated alpha-synuclein. Two substitution mutations (A53T and A30P) in alpha-synuclein gene have been identified in familial early-onset PD. T...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi036114f

    authors： Moussa CE,Wersinger C,Tomita Y,Sidhu A

    更新日期：2004-05-11 00:00:00

  • Direct measurement of the weak interactions between a mouse Fc receptor (Fc gamma RII) and IgG1 in the absence and presence of hapten: a total internal reflection fluorescence microscopy study.

    abstract：:Total internal reflection fluorescence microscopy (TIRFM) has been used to directly measure the weak dissociation constants of IgG with a mouse IgG receptor (moFc gamma RII) that has been purified and reconstituted into substrate-supported planar membranes. Dissociation constants were measured for three different mous...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00161a039

    authors： Hsieh HV,Poglitsch CL,Thompson NL

    更新日期：1992-11-24 00:00:00

  • Photoconversion of 7-dehydrocholesterol to vitamin D3 in synthetic phospholipid bilayers.

    abstract：:The incorporation of 7-dehydrocholesterol into synthetic phospholipid bilayers altered the distribution of products after photolysis. In liposomes, the relative amounts of 7-dehydrocholesterol and lumisterol were elevated, and tachysterol was reduced from the levels observed in hexane solution. Z to E isomerization of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00334a039

    authors： Yamamoto JK,Borch RF

    更新日期：1985-06-18 00:00:00

  • Kinetic characterization of the carbon monoxide-acetyl-CoA (carbonyl group) exchange activity of the acetyl-CoA synthesizing CO dehydrogenase from Clostridium thermoaceticum.

    abstract：:CO dehydrogenase from Clostridium thermoaceticum is a nickel-containing enzyme that catalyzes both the reversible conversion of CO2 to CO (for incorporation into the carbonyl group of acetate) and the synthesis of acetyl-CoA from methyl corrinoid, CO, and CoASH. The latter activity is conveniently assayed by monitorin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00420a019

    authors： Raybuck SA,Bastian NR,Orme-Johnson WH,Walsh CT

    更新日期：1988-10-04 00:00:00

  • Histidine 55 of tryptophan 2,3-dioxygenase is not an active site base but regulates catalysis by controlling substrate binding.

    abstract：:Tryptophan 2,3-dioxygenase (TDO) from Xanthomonas campestris is a highly specific heme-containing enzyme from a small family of homologous enzymes, which includes indoleamine 2,3-dioxygenase (IDO). The structure of wild type (WT TDO) in the catalytically active, ferrous (Fe (2+)) form and in complex with its substrate...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801202a

    authors： Thackray SJ,Bruckmann C,Anderson JL,Campbell LP,Xiao R,Zhao L,Mowat CG,Forouhar F,Tong L,Chapman SK

    更新日期：2008-10-07 00:00:00

  • Mechanism of substrate inactivation of Escherichia coli S-adenosylmethionine decarboxylase.

    abstract：:S-Adenosylmethionine decarboxylase, a pyruvoyl-containing decarboxylase, is inactivated in a time-dependent process under turnover conditions. The inactivation is dependent on the presence of both substrate and Mg2+, which is also required for enzyme activity. The rate of inactivation is dependent on the concentration...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00394a022

    authors： Anton DL,Kutny R

    更新日期：1987-10-06 00:00:00

  • Structure and polymorphism of saturated monoacid 1,2-diacyl-sn-glycerols.

    abstract：:The 1,2-diacyl-sn-glycerols (1,2-DGs) are the predominant naturally occurring isomer found in cell membranes, lipid droplets, and lipoproteins. They are involved in the metabolism of monoacylglycerols, triacylglycerols, and phospholipids. The 1,2-DGs participate in the activation of protein kinase C, in phosphorylatio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00500a008

    authors： Kodali DR,Fahey DA,Small DM

    更新日期：1990-12-04 00:00:00

  • A theory of phase transitions and phase diagrams for one- and two-component phospholipid bilayers.

    abstract：:A statistical mechanical partition function for phospholipid bilayers is constructed to obtain a theoretical description of the chain melting phase transition in lipid bilayer membranes and of the phase diagrams for two-component bilayers. In addition to providing an accurate representation of the transition temperatu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00639a004

    authors： Jacobs RE,Hudson BS,Andersen HC

    更新日期：1977-10-04 00:00:00

  • Allosteric models for multimeric proteins: oxygen-linked effector binding in hemocyanin.

    abstract：:In many crustaceans, changing concentrations of several low molecular weight compounds modulates hemocyanin oxygen binding, resulting in lower or higher oxygen affinities of the pigment. The nonphysiological effector caffeine and the physiological modulator urate, the latter accumulating in the hemolymph of the lobste...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050507s

    authors： Menze MA,Hellmann N,Decker H,Grieshaber MK

    更新日期：2005-08-02 00:00:00

  • Ability of tetrahydrobiopterin analogues to support catalysis by inducible nitric oxide synthase: formation of a pterin radical is required for enzyme activity.

    abstract：:Pterin-free inducible nitric oxide synthase (iNOS) was reconstituted with tetrahydrobiopterin (H(4)B) or tetrahydrobiopterin analogues (5-methyl-H(4)B and 4-amino-H(4)B), and the ability of bound 5-methyl-H(4)B and 4-amino-H(4)B to support catalysis by either full-length iNOS (FLiNOS) or the isolated heme domain (HDiN...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035491p

    authors： Hurshman AR,Krebs C,Edmondson DE,Marletta MA

    更新日期：2003-11-18 00:00:00

  • Molecular properties of p-(dimethylamino)benzaldehyde bound to liver alcohol dehydrogenase: a Raman spectroscopic study.

    abstract：:We have studied the binding nature of an aromatic aldehyde to the catalytic site of liver alcohol dehydrogenase from horse (LADH) using preresonance Raman spectroscopy. The compound p-(dimethylamino)benzaldehyde (DABA) is converted to the corresponding alcohol in the presence of nicotinamide adenine dinucleotide (NADH...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00410a023

    authors： Callender R,Chen D,Lugtenburg J,Martin C,Rhee KW,Sloan D,Vandersteen R,Yue KT

    更新日期：1988-05-17 00:00:00

  • Metabolic changes of membrane lipid composition in Acholeplasma laidlawii by hydrocarbons, alcohols, and detergents: arguments for effects on lipid packing.

    abstract：:The packing of lipids into different aggregates, such as spheres, rods, or bilayers, is dependent on the hydrophobic volume, the hydrocarbon-water interfacial area, and the hydrocarbon chain length of the participating molecules, according to the self-assembly theory [Israelachvili, J. N., Marcelja, S., & Horn, R. G. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00371a038

    authors： Wieslander A,Rilfors L,Lindblom G

    更新日期：1986-11-18 00:00:00

  • Effects of melittin on molecular dynamics and Ca-ATPase activity in sarcoplasmic reticulum membranes: time-resolved optical anisotropy.

    abstract：:We have studied the effect of melittin, a basic membrane-binding peptide, on Ca-ATPase activity and on protein and lipid dynamics in skeletal sarcoplasmic reticulum (SR), using time-resolved phosphorescence and fluorescence spectroscopy. Melittin completely inhibits Ca-ATPase activity, with half-maximal inhibition at ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00244a019

    authors： Voss J,Birmachu W,Hussey DM,Thomas DD

    更新日期：1991-07-30 00:00:00