Identification and synthesis of a naturally occurring selenonucleoside in bacterial tRNAs: 5-[(methylamino)methyl]-2-selenouridine.

abstract：:The transcriptional activity of the hexafluorinated derivative of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], 26,26,26,27,27,27-hexafluoro-1,25-dihydroxyvitamin D3 [F6-1,25-(OH)2D3], was examined in cultured cells by a transient expression assay (CAT assay) using expression vectors for the rat nuclear vitamin D3 receptor...

journal_title：Biochemistry

authors： Sasaki H,Harada H,Handa Y,Morino H,Suzawa M,Shimpo E,Katsumata T,Masuhiro Y,Matsuda K,Ebihara K

更新日期：1995-01-10 00:00:00

abstract：:Achieving a satisfactory biochemical explanation for the opportunistic underwater adhesion of marine invertebrates such as mussels and barnacles requires a detailed characterization of proteins extracted from holdfast structures produced by these organisms. Mefp-5 is an adhesive protein derived from the foot of the co...

journal_title：Biochemistry

authors： Waite JH,Qin X

更新日期：2001-03-06 00:00:00

abstract：:The N-terminal formamido group on imidazole- and pyrrole-containing polyamides causes stacked polyamides to bind in the minor groove of DNA in the staggered motif, and it also increases the binding affinity compared to those of non-formamido compounds. To further investigate the role of the N-terminal acylamido in aff...

journal_title：Biochemistry

authors： Westrate L,Mackay H,Brown T,Nguyen B,Kluza J,Wilson WD,Lee M,Hartley JA

更新日期：2009-06-23 00:00:00

abstract：:The clam Scapharca inaequivalvis possesses two cooperative oxygen binding hemoglobins in its red cells: a homodimeric HbI and a heterotetrameric A2B2 HbII. Each AB dimeric half of HbII is assembled in a manner very similar to that of the well-studied HbI. This study presents crystal structures of HbII along with oxyge...

journal_title：Biochemistry

authors： Ronda L,Bettati S,Henry ER,Kashav T,Sanders JM,Royer WE,Mozzarelli A

更新日期：2013-03-26 00:00:00

abstract：:The primary subsite specificities of human leukocyte elastase, cathepsin G, porcine pancreatic elastase, rat mast cell proteases I and II, bovine chymotrypsin A alpha, and the protease from strain V-8 of Staphylococcus aureus have been mapped with a series of tripeptide thiobenzyl ester substrates of the general formu...

journal_title：Biochemistry

authors： Harper JW,Cook RR,Roberts CJ,McLaughlin BJ,Powers JC

更新日期：1984-06-19 00:00:00

abstract：:Tau is a microtubule associated protein implicated in the pathogenesis of several neurodegenerative diseases. Because of the channel forming properties of other amyloid peptides, we employed planar lipid bilayers and atomic force microscopy to test tau for its ability to form ion permeable channels. Our results demons...

journal_title：Biochemistry

authors： Patel N,Ramachandran S,Azimov R,Kagan BL,Lal R

更新日期：2015-12-22 00:00:00

abstract：:Low-temperature Fourier transform infrared spectroscopy has been used to study mutants of Glu194 and Glu204, two amino acids that are involved in proton release to the extracellular side of bacteriorhodopsin. Difference spectra of films of E194Q, E204Q, E194Q/E204Q, E9Q/E194Q/E204Q, and E9Q/E74Q/E194Q/E204Q at 243, 27...

journal_title：Biochemistry

authors： Lazarova T,Sanz C,Sepulcre F,Querol E,Padrós E

更新日期：2002-06-25 00:00:00

abstract：:We have sought to determine whether insulin can promote cell survival and protect Chinese hamster ovary (CHO) cells from apoptosis induced by serum starvation. Low concentrations of insulin were antiapoptotic for cells overexpressing wild-type insulin receptors but not in cells transfected with kinase-defective insuli...

journal_title：Biochemistry

authors： Lee-Kwon W,Park D,Baskar PV,Kole S,Bernier M

更新日期：1998-11-10 00:00:00

abstract：:Fourteen oligonucleotides 8-21 nucleotides in length and their complements were synthesized as DNA and RNA. For each sequence, four kinds of duplexes, DNA:DNA, RNA:RNA, DNA:RNA, and RNA:DNA, were prepared. Twelve sequences had A.T/U content varying from 25 to 80% and dPy content in the DNA strands varying from 0 to 10...

journal_title：Biochemistry

authors： Lesnik EA,Freier SM

更新日期：1995-08-29 00:00:00

abstract：:The side chain of the antifungal polyketide ansatrienin A produced by Streptomyces collinus contains a cyclohexanecarboxylic acid (CHC) derived moiety. This CHC in the coenzyme A activated form (CHC-CoA) is derived from shikimic acid via a pathway in which the penultimate step is the isomerization of 2-cyclohexenylcar...

journal_title：Biochemistry

authors： Patton SM,Cropp TA,Reynolds KA

更新日期：2000-06-27 00:00:00

abstract：:Beta-catenin phosphorylation at serine 45 (Ser45), threonine 41 (Thr41), Ser37, and Ser33 is critical for beta-catenin degradation, and regulation of beta-catenin phosphorylation is a central part of the canonical Wnt signaling pathway. Beta-catenin mutations at Ser45, Thr41, Ser37, and Ser33 perturb beta-catenin degr...

journal_title：Biochemistry

authors： Wu G,He X

更新日期：2006-04-25 00:00:00

abstract：:A dipeptide containing the binding site for retinal in bovine rhodopsin has been isolated and its sequence determined. Rhodopsin containing [11-3H]retinal was prepared in chromatographically pure form, and the [3H]retinal was reductively linked to its binding site on opsin by using borane--dimethylamine. The [3H]retin...

journal_title：Biochemistry

authors： Wang JK,McDowell JH,Hargrave PA

更新日期：1980-10-28 00:00:00

abstract：:Various agents are able to stimulate the EGF receptor protein tyrosine kinase in the absence of ligand binding. To characterize their mechanism of action, we investigated their effects on the kinase activity of the intracellular domain of the EGF receptor (EGFR-IC). EGFR-IC (67 kDa) lacking the extracellular domain an...

journal_title：Biochemistry

authors： Mohammadi M,Honegger A,Sorokin A,Ullrich A,Schlessinger J,Hurwitz DR

更新日期：1993-08-31 00:00:00

abstract：:S100A1, a member of the S100 protein family, is an EF-hand containing Ca(2+)-binding protein (93 residues per subunit) with noncovalent interactions at its dimer interface. Each subunit of S100A1 has four alpha-helices and a small antiparallel beta-sheet consistent with two helix-loop-helix calcium-binding domains [Ba...

journal_title：Biochemistry

authors： Rustandi RR,Baldisseri DM,Inman KG,Nizner P,Hamilton SM,Landar A,Landar A,Zimmer DB,Weber DJ

更新日期：2002-01-22 00:00:00

abstract：:DNA-DNA interstrand cross-links are thought to be important for the cytotoxicity of many chemotherapeutic agents. To study this more definitively, adduct site-specific methods are used to construct a plasmid with a single nitrogen mustard interstrand cross-link (inter-HN2-pTZSV28). Replication efficiency (RE = [coloni...

journal_title：Biochemistry

authors： Berardini M,Mackay W,Loechler EL

更新日期：1997-03-25 00:00:00

abstract：:The prothrombinase complex assembles through reversible interactions between factor Xa, factor Va and acidic phospholipid-containing membranes in the presence of calcium ions. This complex catalyses the conversion of prothrombin to thrombin through two proteolytic steps. We have used prethrombin 2 as a substrate analo...

journal_title：Biochemistry

authors： Krishnaswamy S,Walker RK

更新日期：1997-03-18 00:00:00

abstract：:Adenosine 5'-phosphosulfate (APS) kinase catalyzes the second reaction in the two-step conversion of inorganic sulfate to 3'-phosphoadenosine 5'-phosphosulfate (PAPS). This report presents the 2.0 A resolution crystal structure of ligand-free APS kinase from the filamentous fungus, Penicillium chrysogenum. The enzyme ...

journal_title：Biochemistry

authors： MacRae IJ,Segel IH,Fisher AJ

更新日期：2000-02-22 00:00:00

abstract：:Arginase is a binuclear manganese metalloenzyme that serves as a therapeutic target for the treatment of asthma, erectile dysfunction, and atherosclerosis. In order to better understand the molecular basis of inhibitor affinity, we have employed site-directed mutagenesis, enzyme kinetics, and X-ray crystallography to ...

journal_title：Biochemistry

authors： Shishova EY,Di Costanzo L,Emig FA,Ash DE,Christianson DW

更新日期：2009-01-13 00:00:00

abstract：:The reduction kinetics of cytochrome P-450 are known to be biphasic, with a rapid initial phase and a slower subsequent phase, both of which appear linear in semilogarithmic plots. The present report demonstrates that these biphasic reduction kinetics can be described in terms of a preequilibrium between high- and low...

journal_title：Biochemistry

authors： Backes WL,Sligar SG,Schenkman JB

更新日期：1982-03-16 00:00:00

abstract：:An alamethicin, secreted by the fungus Trichoderma viride and containing a glutamine at position 18 instead of the usual glutamic acid, has been uniformly labeled with 15N and purified by HPLC. The extent of 15N incorporation at individual backbone and side-chain sites was found to vary from 85% to 92%, as measured by...

journal_title：Biochemistry

authors： Yee AA,O'Neil JD

更新日期：1992-03-31 00:00:00

abstract：:Translation of mRNAs by the ribosome is stereospecific, with only l-amino acids being incorporated into the nascent polypeptide chain. This stereospecificity results from the exclusion of d-amino acids at three steps during protein synthesis: (1) the aminoacylation of tRNA by aminoacyl-tRNA synthetases, (2) binding of...

journal_title：Biochemistry

authors： Richardson CJ,First EA

更新日期：2016-03-15 00:00:00

abstract：:Initial rate kinetic studies and reduced coenzyme binding studies with bovine glutamate dehydrogenase have shown that an enzyme-NAD(P)H-glutamate abortive complex is a major participant in the overall enzyme-catalyzed reaction. The allosteric regulator ADP is shown to activate the enzyme by destabilizing this abortive...

journal_title：Biochemistry

authors： George A,Bell JE

更新日期：1980-12-23 00:00:00

abstract：:An important subject for elucidating membrane protein (MP) folding is how transmembrane helices (TMHs) insert into and dissociate from membranes. We investigated helix dissociation kinetics and insertion topology by means of intervesicular transfer of the fluorophore-labeled completely hydrophobic model transmembrane ...

journal_title：Biochemistry

authors： Yano Y,Matsuzaki K

更新日期：2002-10-15 00:00:00

abstract：:The (beta alpha)8 (or "TIM")-barrel protein phosphoribosylanthranilate isomerase from Saccharomyces cerevisiae was cleaved between the sixth and seventh beta alpha module to test the capacity of the resulting fragments to adopt native format autonomously. The fragments, which were expressed from separate coding sequen...

journal_title：Biochemistry

authors： Eder J,Kirschner K

更新日期：1992-04-14 00:00:00

abstract：:The mammalian HIRA/UBN1/CABIN1/ASF1a (HUCA) histone chaperone complex deposits the histone H3 variant H3.3 into chromatin and is linked to gene activation, repression, and chromatin assembly in diverse cell contexts. We recently reported that a short N-terminal fragment of UBN1 containing amino acids 1-175 is necessar...

journal_title：Biochemistry

authors： Tang Y,Puri A,Ricketts MD,Rai TS,Hoffmann J,Hoi E,Adams PD,Schultz DC,Marmorstein R

更新日期：2012-03-27 00:00:00

abstract：:Weak or nonexistent smallpox immunity in today's human population raises concerns about the possibility of natural or provoked genetic modifications leading to re-emergence of variola virus and other poxviruses. Thus, the development of new antiviral strategies aimed at poxvirus infections in humans is a high priority...

journal_title：Biochemistry

authors： Duraffour S,Ishchenko AA,Saparbaev M,Crance JM,Garin D

更新日期：2007-10-23 00:00:00

abstract：:The monoamine transporter of the chromaffin granule membranes can be specifically labeled by the photoaffinity reagent 7-azido-8-[125I]iodoketanserin. The characteristics of the labeled protein have been investigated. Two-dimensional gel electrophoresis of the labeled membranes indicated a MW of about 70,000 and an is...

journal_title：Biochemistry

authors： Isambert MF,Gasnier B,Botton D,Henry JP

更新日期：1992-02-25 00:00:00

abstract：:Derivatives of imidazole-2-thiones block reactions catalyzed by thyroid peroxidase (TPX) and the closely related lactoperoxidase (LPX), and this property is used therapeutically to treat hyperthyroidism. The reactions of a series of benzimidazoline-2-thiones with chemical and enzymatic oxidants were investigated to pr...

journal_title：Biochemistry

authors： Doerge DR,Decker CJ,Takazawa RS

更新日期：1993-01-12 00:00:00

abstract：:Pulsed electron paramagnetic resonance spectroscopy has been used to detect Mn(II)-ligand superhyperfine couplings in complexes with creatine kinase and in the Mn(II) metalloprotein concanavalin A. Electron spin-echo envelopes from Mn(II), bound in these complexes, are modulated by superhyperfine interactions between ...

journal_title：Biochemistry

authors： LoBrutto R,Smithers GW,Reed GH,Orme-Johnson WH,Tan SL,Leigh JS Jr

更新日期：1986-09-23 00:00:00

abstract：:Eukaryotic translation initiation factor 2B (eIF2B) is the guanine nucleotide exchange factor of the GTPase eIF2, which brings the initiator Met-tRNAi to the ribosome in the form of the eIF2-GTP·Met-tRNAi ternary complex (TC). The activity of eIF2B is inhibited by phosphorylation of its substrate eIF2 by several stres...

journal_title：Biochemistry

authors： Bogorad AM,Lin KY,Marintchev A

更新日期：2018-03-06 00:00:00