Chemical and enzymatic oxidation of benzimidazoline-2-thiones: a dichotomy in the mechanism of peroxidase inhibition.

abstract：:Beta-amylase (EC 3.2.1.2) is starch-hydrolyzing exo-type enzyme that can catalyze the successive liberation of beta-maltose from the nonreducing ends of alpha-1,4-linked glucopyranosyl polymers. There is a well-known phenomenon called multiple or repetitive attack where the enzyme releases several maltose molecules in...

journal_title：Biochemistry

authors： Ishikawa K,Nakatani H,Katsuya Y,Fukazawa C

更新日期：2007-01-23 00:00:00

abstract：:We have separately expressed the Dictyosteliumdiscoideum myosin II nonhydrolyzer point mutations E459V and E476K [Ruppel, K. M., and Spudich, J. A. (1996) Mol. Biol. Cell 7, 1123-1136] in the soluble myosin head fragment M761-1R [Anson et al. (1996) EMBO J. 15, 6069-6074] and performed transient kinetic analyses to ch...

journal_title：Biochemistry

authors： Friedman AL,Geeves MA,Manstein DJ,Spudich JA

更新日期：1998-07-07 00:00:00

abstract：:Strong absorbance of water at 1645 cm-1 has previously prevented dependable observation of infrared spectra of H2O solutions in the carbonyl region. We have obtained reliable spectra in pure water and in alcohol-water mixtures in this region and used them to study A.U polynucleotide interaction. Spectra in pure water ...

journal_title：Biochemistry

authors： Miles HT,Frazier J

更新日期：1978-07-11 00:00:00

abstract：:High signal-to-noise Fourier transform infrared (FTIR) spectra of the 5-hydroxytryptamine (serotonin) receptor (5-HT(3)R) and the nicotinic acetylcholine receptor (nAChR) were obtained by microscope FTIR spectroscopy using micrometer-sized, fully hydrated protein films. Because this novel procedure requires only nanog...

journal_title：Biochemistry

authors： Rigler P,Ulrich WP,Hovius R,Ilegems E,Pick H,Vogel H

更新日期：2003-12-02 00:00:00

abstract：:Formation of protein-protein assemblies is essential in maintaining cell structure and function. Conservation of structural motifs and binding sites is the result of evolutionary pressure for solutions compatible with both molecular economy and regulation. PDZ domains are a typical example: A conserved fold governs sp...

journal_title：Biochemistry

authors： Milev S,Bjelić S,Georgiev O,Jelesarov I

更新日期：2007-01-30 00:00:00

abstract：:Protein disulfide isomerase (PDI) utilizes the active site sequence Cys-Gly-His-Cys (CGHC; E degrees ' = -180 mV) to effect thiol-disulfide interchange during oxidative protein folding. Here, the Cys-Gly-Cys-NH(2) (CGC) peptide is shown to have a disulfide reduction potential (E degrees ' = -167 mV) that is close to t...

journal_title：Biochemistry

authors： Woycechowsky KJ,Raines RT

更新日期：2003-05-13 00:00:00

abstract：:It is known that the proapoptotic protein Bax facilitates the formation of pores in bilayers, resulting in the release of proteins from the intermitochondrial space. We demonstrate that another consequence of the interaction of Bax with membranes is an increase in the rate of lipid transbilayer diffusion. We use two i...

journal_title：Biochemistry

authors： Epand RF,Martinou JC,Montessuit S,Epand RM

更新日期：2003-12-16 00:00:00

abstract：:X-ray crystallographic studies have shown that DNA binding by human polymerase beta (pol beta) occurs primarily through two structurally and sequentially homologous helix-hairpin-helix (HhH) motifs, one in the fingers subdomain and the other in the 8-kDa domain [Pelletier, H., Sawaya, M. R., Wolfle, W., Wilson, S. H.,...

journal_title：Biochemistry

authors： Pelletier H,Sawaya MR

更新日期：1996-10-01 00:00:00

abstract：:The "stalk" of the prokaryotic 50S ribosomal subunit is comprised of four copies of the protein L7/L12. In Escherichia coli, L7/L12 is a dimeric protein at micromolar concentrations, which is able to undergo rapid subunit exchange. A recent structural study indicated a tetrameric arrangement of the L12 proteins isolat...

journal_title：Biochemistry

authors： Moens PD,Wahl MC,Jameson DM

更新日期：2005-03-08 00:00:00

abstract：:Basic peptides from the carboxy terminus of the human immunodeficiency virus type 1 (HIV-1) Tat protein bind to the stem-loop region of TAR RNA, spanning a trinucleotide bulge, with high affinity and moderate specificity. Previous studies have demonstrated that TAR RNA contains a specific arginine binding pocket. A se...

journal_title：Biochemistry

authors： Long KS,Crothers DM

更新日期：1995-07-11 00:00:00

abstract：:We have characterized by EPR the interaction of the Em,7 = -50 mV [4Fe-4S] cluster of Escherichia coli DMSO reductase (DmsABC) with a menaquinol (MQH2) binding site. Potentiometric titrations indicate that in DmsAB(C102S)C, the Em,7 = -50 mV [4Fe-4S] cluster is replaced by an Em,7 = +260 mV [3Fe-4S] cluster. The Q-poo...

journal_title：Biochemistry

authors： Rothery RA,Weiner JH

更新日期：1996-03-12 00:00:00

abstract：:Despite many studies suggesting the role of enolpyruvate as a bound intermediate in the pyruvate kinase reaction, direct evidence for it has been lacking. By use of a combination of chemical trapping and isolation of a derivative, significant amounts of enzyme-bound enolpyruvate have now been demonstrated. The method ...

journal_title：Biochemistry

authors： Seeholzer SH,Jaworowski A,Rose IA

更新日期：1991-01-22 00:00:00

abstract：:Fibrinogen is a ligand for leukocyte integrin alpha(M)beta2 (CD11b/CD18, Mac-1) and mediates adhesion and migration of leukocytes during the immune-inflammatory responses. The binding site for alpha(M)beta2 resides in gammaC, a constituent subdomain in the D-domain of fibrinogen. The sequence gamma383-395 (P2-C) in ga...

journal_title：Biochemistry

authors： Lishko VK,Kudryk B,Yakubenko VP,Yee VC,Ugarova TP

更新日期：2002-10-29 00:00:00

abstract：:Nitrosylhemoglobin (HbFe(II)NO) has been shown to be generated in vivo from the reaction of deoxyHb with NO(*) as well as with nitrite. Despite the physiological importance attributed to this form of Hb, its reactivity has not been investigated in detail. In this study, we showed that the rate of oxidation of HbFe(II)...

journal_title：Biochemistry

authors： Herold S,Röck G

更新日期：2005-04-26 00:00:00

abstract：:Furamidine and related diamidines represent a promising series of drugs active against widespread parasites, in particular the Pneumocystic carinii pathogen. In this series, the phenylfuranbenzimidazole diamidine derivative DB293 was recently identified as the first unfused aromatic dication capable of forming stacked...

journal_title：Biochemistry

authors： Bailly C,Tardy C,Wang L,Armitage B,Hopkins K,Kumar A,Schuster GB,Boykin DW,Wilson WD

更新日期：2001-08-21 00:00:00

abstract：:Gap junctions, transmembrane protein channels that directly connect the cytoplasm of neighboring cells and enable the exchange of molecules between cells, are a promising new frontier for therapeutic delivery. Specifically, cell-derived lipid vesicles that contain functional gap junction channels, termed Connectosomes...

journal_title：Biochemistry

authors： Gadok AK,Zhao C,Meriwether AI,Ferrati S,Rowley TG,Zoldan J,Smyth HDC,Stachowiak JC

更新日期：2018-01-09 00:00:00

abstract：:The enzyme endonuclease V initiates repair of deaminated DNA bases by making an endonucleolytic incision on the 3' side one nucleotide from a base lesion. In this study, we have used site-directed mutagenesis to characterize the role of the highly conserved residues D43, E89, D110, and H214 in Thermotoga maritima endo...

journal_title：Biochemistry

authors： Feng H,Dong L,Cao W

更新日期：2006-08-29 00:00:00

abstract：:The maltodextrin binding protein from Escherichia coli serves as the initial receptor for both the active transport of and chemotaxis toward a range of linear maltose sugars. The X-ray structures of both the maltose-bound and sugar-free forms of the protein have been previously described [Spurlino, J. C., Lu, G.-Y., &...

journal_title：Biochemistry

authors： Sharff AJ,Rodseth LE,Quiocho FA

更新日期：1993-10-12 00:00:00

abstract：:Intermolecular triplex DNA is stabilized by metal cations and polyamines which reduce repulsion between the negatively charged phosphates of the three nucleic acid strands. We use a quantitative chemical-probing assay involving protection of duplex guanines in a homopyrimidine.homopurine (Py.Pu) sequence from dimethyl...

journal_title：Biochemistry

authors： Potaman VN,Sinden RR

更新日期：1995-11-14 00:00:00

abstract：:Proton NMR studies were conducted on the complementary 15-mer duplex d(5'-TACTCTTCTTGACCT).(5'-AGGTCAAGAAGAGTA) (designated as unmodified 15-mer duplex) spanning a portion of the mouse c-Ha-ras protooncogene centered around codon 61. Identical studies were carried out on the same sequence, after specific modification ...

journal_title：Biochemistry

authors： Cho BP,Beland FA,Marques MM

更新日期：1992-10-13 00:00:00

abstract：:Endogenous DNA damage occurs at a rate of at least 20,000 lesions per cell per day. Base excision repair (BER) is a key pathway for maintaining genome stability. Several pol beta variants were identified as conferring resistance to 3'-azido-3'-deoxythymidine (AZT) in Escherichia coli (Kosa et al. (1999) J. Biol. Chem....

journal_title：Biochemistry

authors： Murphy DL,Kosa J,Jaeger J,Sweasy JB

更新日期：2008-08-05 00:00:00

abstract：:We used a combination of optical and calorimetric techniques to investigate the incorporation of deoxythymidine --> deoxyuridine (dT --> dU) substitutions in the duplex and third strand of the parallel intramolecular triplex d(A(7)C(5)T(7)C(5)T(7)) (ATT). UV and differential scanning calorimetry melting experiments sh...

journal_title：Biochemistry

authors： Soto AM,Rentzeperis D,Shikiya R,Alonso M,Marky LA

更新日期：2006-03-07 00:00:00

abstract：:In animals, the post-translational hydroxylation of hypoxia inducible factor (HIF) is a central mechanism for regulating gene expression in an oxygen-dependent manner. The oxygenase-catalyzed trans-4-prolyl hydroxylation of HIF-alpha increases its affinity for the von Hippel Lindau protein elongin B/C (VCB) ubiquitin ...

journal_title：Biochemistry

authors： Illingworth CJ,Loenarz C,Schofield CJ,Domene C

更新日期：2010-08-17 00:00:00

abstract：:Amyloid diseases are caused by the self-assembly of a given protein into an insoluble cross-beta-sheet quaternary structural form which is pathogenic. An understanding of the biochemical mechanism of amyloid fibril formation should prove useful in understanding amyloid disease. Toward this end, a procedure for the con...

journal_title：Biochemistry

authors： Colon W,Kelly JW

更新日期：1992-09-15 00:00:00

abstract：:The innate immune response to infection or injury involves an antigen-antibody triggered classical pathway (CP) of complement activation, in which soluble and cell surface plasma proteins cooperatively effect elimination of foreign organisms and damaged host cells. However, protracted or dysfunctional complement activ...

journal_title：Biochemistry

authors： Halili MA,Ruiz-Gómez G,Le GT,Abbenante G,Fairlie DP

更新日期：2009-09-08 00:00:00

abstract：:N-Acyl ethanolamines (NAEs) constitute a large and diverse class of signaling lipids that includes the endogenous cannabinoid anandamide. Like other lipid transmitters, NAEs are thought to be biosynthesized and degraded on-demand rather than being stored in vesicles prior to signaling. The identification of enzymes in...

journal_title：Biochemistry

authors： Leung D,Saghatelian A,Simon GM,Cravatt BF

更新日期：2006-04-18 00:00:00

abstract：:The initiation of Escherichia coli chromosomal replication by DnaA protein is strongly influenced by the tight binding of the nucleotides ATP and ADP. Anionic phospholipids in a fluid bilayer promote the conversion of inactive ADP-DnaA protein to replicatively active ATP-DnaA protein in vitro, and thus likely play a k...

journal_title：Biochemistry

authors： Kitchen JL,Li Z,Crooke E

更新日期：1999-05-11 00:00:00

abstract：:The DNA strand cleavage reaction catalyzed by endonuclease III from Escherichia coli (endo III) on the 3'-side of aldehyde abasic sites proceeds by a syn beta-elimination involving abstraction of the 2'-pro-S proton and formation of a trans alpha,beta-unsaturated aldose product; we previously reported the same stereoc...

journal_title：Biochemistry

authors： Mazumder A,Gerlt JA,Absalon MJ,Stubbe J,Cunningham RP,Withka J,Bolton PH

更新日期：1991-01-29 00:00:00

abstract：:The water-oxidizing complex of photosystem II cycles through five oxidation states, denoted S(i)() (i = 0-4), during water oxidation to molecular oxygen, which appears at the (transient) S(4) state. The recent detection of bimodal EPR signals from the S(3) state [Matsukawa, T., Mino, H., Yoneda, D., Kawamori, A. (1999...

journal_title：Biochemistry

authors： Ioannidis N,Petrouleas V

更新日期：2002-07-30 00:00:00

abstract：:The kinetic and thermodynamic features of reactions catalyzed by present-day enzymes appear to be the consequence of the evolution of these proteins toward maximal catalytic effectiveness. These features are identified and analyzed (in detail for one substrate-one product enzymes) by using ideas that link the energeti...

journal_title：Biochemistry

authors： Burbaum JJ,Raines RT,Albery WJ,Knowles JR

更新日期：1989-11-28 00:00:00