Stereochemical studies of the beta-elimination reactions at aldehydic abasic sites in DNA: endonuclease III from Escherichia coli, sodium hydroxide, and Lys-Trp-Lys.

abstract：:Quasi-elastic laser light scattering is used to examine the cholera toxin mediated agglutination of ganglioside GM1 containing phospholipid vesicles and Gm1-coated polystyrene spheres. We find that the ability of cholera toxin to agglutinate Gm1-containing phospholipid vesicles depends markedly on the lipid compositio...

journal_title：Biochemistry

authors： Dwyer JD,Bloomfield VA

更新日期：1982-06-22 00:00:00

abstract：:Benzoylformate decarboxylase (BFDC) and pyruvate decarboxylase (PDC) are both thiamin diphosphate-dependent enzymes. The two share a common three-dimensional structure and catalyze a similar chemical reaction, i.e., decarboxylation of 2-keto acids. However, they vary significantly in their substrate utilization patter...

journal_title：Biochemistry

authors： Yep A,McLeish MJ

更新日期：2009-09-08 00:00:00

abstract：:Although the addition of various divalent metals to beta-galactosidase resulted in apparent activation, only Mg2+ and Mn2+ actually did activate. The apparent activation by the other divalent metals was shown to be due to Mg2+ impurities. Calcium did not activate, but experiments suggested that it did bind. Other diva...

journal_title：Biochemistry

authors： Huber RE,Parfett C,Woulfe-Flanagan H,Thompson DJ

更新日期：1979-09-18 00:00:00

abstract：:In eukaryotic cells, chromatin is classified into euchromatin, which is active in transcription, and heterochromatin that silences transcription. Histones in these two domains contain distinct modifications. Chromatin assembly factor 1 (CAF-1) is a highly conserved protein that functions in DNA replication, DNA repair...

journal_title：Biochemistry

authors： Zhou H,Madden BJ,Muddiman DC,Zhang Z

更新日期：2006-03-07 00:00:00

abstract：:Coiled coils are simple models for studying the association of two polypeptide chains to form a folded protein. Previous work has shown that the folding of a coiled coil can be described by a two-state transition between two unfolded monomeric peptide chains and a folded coiled coil dimer. Here we report the thermodyn...

journal_title：Biochemistry

authors： Bosshard HR,Dürr E,Hitz T,Jelesarov I

更新日期：2001-03-27 00:00:00

abstract：:In halophilic archaea the photophobic response is mediated by the membrane-embedded 2:2 photoreceptor/-transducer complex SRII/HtrII, the latter being homologous to the bacterial chemoreceptors. Both systems bias the rotation direction of the flagellar motor via a two-component system coupled to an extended cytoplasmi...

journal_title：Biochemistry

authors： Orban-Glaß I,Voskoboynikova N,Busch KB,Klose D,Rickert C,Mosslehy W,Roder F,Wilkens V,Piehler J,Engelhard M,Steinhoff HJ,Klare JP

更新日期：2015-01-20 00:00:00

abstract：:Integrin αIIbβ3, a transmembrane heterodimer, mediates platelet aggregation when it switches from an inactive to an active ligand-binding conformation following platelet stimulation. Central to regulating αIIbβ3 activity is the interaction between the αIIb and β3 extracellular stalks, which form a tight heterodimer in...

journal_title：Biochemistry

authors： Tan SK,Fong KP,Polizzi NF,Sternisha A,Slusky JSG,Yoon K,DeGrado WF,Bennett JS

更新日期：2019-07-30 00:00:00

abstract：:The thermodynamics of the interactions of serum apotransferrin (T) and holotransferrin (TFe(2)) with ceruloplasmin (Cp), as well as those of human lactoferrin (Lf), were assessed by fluorescence emission spectroscopy. Cp interacts with two Lf molecules. The first interaction depends on pH and μ, whereas the second doe...

journal_title：Biochemistry

authors： Ha-Duong NT,Eid C,Hémadi M,El Hage Chahine JM

更新日期：2010-12-07 00:00:00

abstract：:A number of isomeric diacylglycerophosphocholines and diacylglycero sulfates containing O-acyl and/or S-acyl ester bonds were investigated as substrates for porcine pancreatic phospholipase A2 and its zymogen. A comparison is made with the kinetic properties of the enzyme toward the corresponding glycol detergents pre...

journal_title：Biochemistry

authors： van Oort MG,Dijkman R,Hille JD,de Haas GH

更新日期：1985-12-31 00:00:00

abstract：:A cyclic nucleotide phosphodiesterase was extensively purified from the 100000g supernatant fraction of human platelets. The purification was 2500-3000-fold with 30% recovery of activity. The enzyme was isolated by DEAE-cellulose chromatography followed by adsorption to blue dextran-Sepharose and elution with cAMP. Th...

journal_title：Biochemistry

authors： Grant PG,Colman RW

更新日期：1984-04-10 00:00:00

abstract：:In iron-deficient conditions, Azomonas macrocytogenes ATCC 12334 excretes a fluorescent siderophore called azoverdin, which is composed of a six-amino-acid peptide chain linked to a chromophore. Azoverdin chelates iron(III) very strongly, solubilizing it and transporting it back into the cells using an outer-membrane ...

journal_title：Biochemistry

authors： Wasielewski E,Atkinson RA,Abdallah MA,Kieffer B

更新日期：2002-10-15 00:00:00

abstract：:The technique of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) has been applied to a mesophilic (E. coli) dihydrofolate reductase under conditions that allow direct comparison to a thermophilic (B. stearothermophilus) ortholog, Ec-DHFR and Bs-DHFR, respectively. The analysis of hydrogen-deuterium e...

journal_title：Biochemistry

authors： Oyeyemi OA,Sours KM,Lee T,Kohen A,Resing KA,Ahn NG,Klinman JP

更新日期：2011-09-27 00:00:00

abstract：:We have performed microsecond (μs) molecular dynamics simulation (MDS) to identify structural mechanisms for sarcolipin (SLN) uncoupling of Ca2+ transport from ATP hydrolysis for the sarcoplasmic reticulum Ca2+-ATPase (SERCA). SLN regulates muscle metabolism and energy expenditure to provide resistance against diet-in...

journal_title：Biochemistry

authors： Autry JM,Thomas DD,Espinoza-Fonseca LM

更新日期：2016-11-08 00:00:00

abstract：:Caveolae are approximately 50-100 nm invaginations of the plasma membrane thought to form as a result of a local accumulation of cholesterol, sphingolipids, and a unique family of three proteins known as the caveolins: Cav-1, -2, and -3. Here, we report the identification, sequence, and developmental expression of the...

journal_title：Biochemistry

authors： Razani B,Park DS,Miyanaga Y,Ghatpande A,Cohen J,Wang XB,Scherer PE,Evans T,Lisanti MP

更新日期：2002-06-25 00:00:00

abstract：:Crystallographic studies of ligand-protein complexes reveal most preferable ligand binding modes, but do not show less populated modes that may contribute to measurable biochemical and biophysical characteristics of the complexes. In some cases, a ligand may bind a protein in essentially different modes. An example is...

journal_title：Biochemistry

authors： Blanchet J,Lin SX,Zhorov BS

更新日期：2005-05-17 00:00:00

abstract：:Serine palmitoyltransferase (EC 2.3.1.50) catalyzes the condensation of L-serine and palmitoyl-CoA to yield 3-ketosphinganine in the first unique reaction of long-chain (sphingoid) base biosynthesis. The kinetic effects of changing the extracellular concentrations of the precursors for this pathway were studied with L...

journal_title：Biochemistry

authors： Merrill AH Jr,Wang E,Mullins RE

更新日期：1988-01-12 00:00:00

abstract：:P-glycoprotein (P-gp) is the most well-known ATP-binding cassette (ABC) transporter involved in unidirectional substrate translocation across the membrane lipid bilayer, thereby causing the typical multidrug resistance (MDR) phenotype expressed in many cancers. We observed that in human CEM acute lymphoblastic leukemi...

journal_title：Biochemistry

authors： Gayet L,Dayan G,Barakat S,Labialle S,Michaud M,Cogne S,Mazane A,Coleman AW,Rigal D,Baggetto LG

更新日期：2005-03-22 00:00:00

abstract：:Binding of the N-acetyllactosamine-specific lectin from Erythrina corallodendron (ECorL) to four glycosphingolipids has been tested using the microtiter well assay. The role of several amino acids in the binding site region was studied by combining binding assays and molecular modeling for native and recombinant forms...

journal_title：Biochemistry

authors： Moreno E,Teneberg S,Adar R,Sharon N,Karlsson KA,Angström J

更新日期：1997-04-15 00:00:00

abstract：:There are four isoforms of centrin in mammals, with variable sequence, tissue expression, and functional properties. We have recently characterized a number of structural, ion, and target binding properties of human centrin isoform HsCen2. This paper reports a similar characterization of HsCen3, overexpressed in Esche...

journal_title：Biochemistry

authors： Cox JA,Tirone F,Durussel I,Firanescu C,Blouquit Y,Duchambon P,Craescu CT

更新日期：2005-01-25 00:00:00

abstract：:Aerobic and facultative bacteria and archaea harboring mer loci exhibit resistance to the toxic effects of Hg(II) and organomercurials [RHg(I)]. In broad spectrum resistance, RHg(I) is converted to less toxic Hg(0) in the cytosol by the sequential action of organomercurial lyase (MerB: RHg(I) → RH + Hg(II)) and mercur...

journal_title：Biochemistry

authors： Hong B,Nauss R,Harwood IM,Miller SM

更新日期：2010-09-21 00:00:00

abstract：:An OxlT homology model suggests R272 and K355 in transmembrane helices 8 and 11, respectively, are critical to OxlT-mediated transport. We offer positive evidence supporting this idea by studying OxlT function after cysteine residues were separately introduced at these positions. Without further treatment, both mutant...

journal_title：Biochemistry

authors： Wang X,Sarker RI,Maloney PC

更新日期：2006-08-29 00:00:00

abstract：:A major challenge to the study of protein folding is the fact that intermediate states along the reaction pathway are generally unstable and thus difficult to observe. Recently developed NMR relaxation dispersion experiments present an avenue to accessing such states, providing kinetic, thermodynamic, and structural i...

journal_title：Biochemistry

authors： Mittermaier A,Korzhnev DM,Kay LE

更新日期：2005-11-29 00:00:00

abstract：:Homoserine transacetylase catalyzes one of the required steps in the biosynthesis of methionine in fungi and several bacteria. We have determined the crystal structure of homoserine transacetylase from Haemophilus influenzae to a resolution of 1.65 A. The structure identifies this enzyme to be a member of the alpha/be...

journal_title：Biochemistry

authors： Mirza IA,Nazi I,Korczynska M,Wright GD,Berghuis AM

更新日期：2005-12-06 00:00:00

abstract：:Furamidine and related diamidines represent a promising series of drugs active against widespread parasites, in particular the Pneumocystic carinii pathogen. In this series, the phenylfuranbenzimidazole diamidine derivative DB293 was recently identified as the first unfused aromatic dication capable of forming stacked...

journal_title：Biochemistry

authors： Bailly C,Tardy C,Wang L,Armitage B,Hopkins K,Kumar A,Schuster GB,Boykin DW,Wilson WD

更新日期：2001-08-21 00:00:00

abstract：:The major excreted protein (MEP) of transformed mouse fibroblasts has been purified, and monospecific antisera against it have been prepared. Synthesis and secretion of this protein have previously been shown to be stimulated by transformation or treatment with tumor-promoting phorbol esters, but its function is still...

journal_title：Biochemistry

authors： Gottesman MM,Cabral F

更新日期：1981-03-17 00:00:00

abstract：:The present paper reports a study on the equilibria and kinetics of the acid-alkaline transition and the azide binding reaction by ferric Aplysia myoglobin. A single completely reversible spectrophotometric titration curve is found over the pH range from similar to 5 to similar to 9, with an apparent pK equals to 7.5 ...

journal_title：Biochemistry

authors： Giacometti GM,Da Ros A,Antonini E,Brunori M

更新日期：1975-04-22 00:00:00

abstract：:Avian magnetoreception is assumed to occur in the retina. Although its molecular mechanism is unclear, magnetic field-dependent formation and the stability of radical-containing photointermediate(s) are suggested to play key roles in a hypothesis called the radical pair mechanism. Chicken cryptochrome4 (cCRY4) has bee...

journal_title：Biochemistry

authors： Otsuka H,Mitsui H,Miura K,Okano K,Imamoto Y,Okano T

更新日期：2020-09-29 00:00:00

abstract：:We have combined three mutations previously shown to stabilize lambda repressor against thermal denaturation. Two of these mutations are in helix 3, where Gly-46 and Gly-48 have been replaced by alanines [Hecht, M. H., et al. (1986) Proteins: Struct., Funct., Genet. 1, 43-46]. The other mutation, which replaces Tyr-88...

journal_title：Biochemistry

authors： Stearman RS,Frankel AD,Freire E,Liu BS,Pabo CO

更新日期：1988-09-20 00:00:00

abstract：:CNAD (5-beta-D-ribofuranosylnicotinamide adenine dinucleotide) is an isosteric C-glycosidic analogue of NAD(H) containing a neutral pyridine ring. CPAD (5-beta-D-ribofuranosylpicolinamide adenine dinucleotide) is a closely related pyridine-containing analogue with the pyridine nitrogen on the opposite side of the ring...

journal_title：Biochemistry

authors： Li H,Hallows WH,Punzi JS,Pankiewicz KW,Watanabe KA,Goldstein BM

更新日期：1994-10-04 00:00:00

abstract：:Conformational changes in the prion protein cause transmissible spongiform encephalopathies, also referred to as prion diseases. In its native state, the prion protein is innocuous (PrPC), but it can misfold into a neurotoxic and infectious isoform (PrPSc). The full-length cellular form of the prion protein consists o...

journal_title：Biochemistry

authors： DeMarco ML,Daggett V

更新日期：2007-03-20 00:00:00