Downscaling Fourier transform infrared spectroscopy to the micrometer and nanogram scale: secondary structure of serotonin and acetylcholine receptors.

abstract：:To identify the elements which regulate the liver transcription of the human type II phospholipase A2 gene and its stimulation by interleukin 6, the 5' flanking region from -1614 to +806 and several 3' and 5' deleted fragments have been analyzed in CAT assays. Negative regulatory elements have been located in the regi...

journal_title：Biochemistry

authors： Olivier JL,Fan Q,Salvat C,Ziari M,Kong L,Mangeney M,Bereziat G

更新日期：1994-06-14 00:00:00

abstract：:DADH catalyzes the flavin-dependent oxidative deamination of d-amino acids to the corresponding α-keto acids and ammonia. Here we report the first X-ray crystal structures of DADH at 1.06 Å resolution and its complexes with iminoarginine (DADH(red)/iminoarginine) and iminohistidine (DADH(red)/iminohistidine) at 1.30 Å...

journal_title：Biochemistry

authors： Fu G,Yuan H,Li C,Lu CD,Gadda G,Weber IT

更新日期：2010-10-05 00:00:00

abstract：:The electron density map of the 3D crystal of Photosystem II from Thermosynechococcus vulcanus with a 1.9 Å resolution (PDB: 3ARC ) exhibits, in the two monomers in the asymmetric unit cell, an, until now, unidentified and uninterpreted strong difference in electron density centered at a distance of around 1.5 Å from ...

journal_title：Biochemistry

authors： Sugiura M,Koyama K,Umena Y,Kawakami K,Shen JR,Kamiya N,Boussac A

更新日期：2013-12-31 00:00:00

abstract：:The mitochondrial tyrosyl tRNA synthetase from Neurospora crassa (CYT-18 protein) is a bifunctional group I intron splicing cofactor. CYT-18 is capable of splicing multiple group I introns from a wide variety of sources by stabilizing the catalytically active intron structures. CYT-18 and mt TyrRSs from related fungal...

journal_title：Biochemistry

authors： Geng C,Paukstelis PJ

更新日期：2014-03-04 00:00:00

abstract：:The channel of the purified acetylcholine receptor from Torpedo californica electric organ reconstituted in lipid vesicles was assayed by direct electrical recording using patch-clamp pipets. High-resistance seals were obtained by gentle suction of vesicles into the pipet or after the formation of lipid bilayers from ...

journal_title：Biochemistry

authors： Suarez-Isla BA,Wan K,Lindstrom J,Montal M

更新日期：1983-05-10 00:00:00

abstract：:Resonances of the histidine region of human carbonic anhydrase B have been studied by proton magnetic resonance spectroscopy in the presence of seven sulfonamide inhibitors. Results of difference spectroscopy and observation of the C-2 resonance of an additional titratable histidine in some of these spectra suggest a ...

journal_title：Biochemistry

authors： Pesando JM,Grollman AP

更新日期：1975-02-25 00:00:00

abstract：:The hepatic metabolism of deuteriated D-methionine has been studied in the intact, anesthetized rat using 2H NMR spectroscopy. The rate of formation of the principal labeled metabolite, [methyl-2H3]sarcosine, from the D-[methyl-2H3]methionine precursor was found to be as rapid as the rate observed previously in NMR st...

journal_title：Biochemistry

authors： London RE,Gabel SA

更新日期：1988-10-04 00:00:00

abstract：:We previously showed that estrogen-related receptor alpha1 (ERRalpha1) can compete with estrogen receptor alpha (ERalpha) for binding to estrogen response elements (EREs), repressing transcription in the mammary carcinoma cell line MCF-7. Given that ERRalpha1 can function in the absence of ligands and exists as a phos...

journal_title：Biochemistry

authors： Vu EH,Kraus RJ,Mertz JE

更新日期：2007-08-28 00:00:00

abstract：:Molecular chaperones of the heat shock protein 70 (Hsp70) family counteract protein misfolding in a variety of neurodegenerative disease models. To determine whether human Hsp70 exerts similar effects on the aggregation of alpha-synuclein (alpha-Syn), the key component of insoluble fibrils present in Parkinson's disea...

journal_title：Biochemistry

authors： Luk KC,Mills IP,Trojanowski JQ,Lee VM

更新日期：2008-11-25 00:00:00

abstract：:We consider the problem of color regulation in visual pigments for both bovine rhodopsin (lambda max = 500 nm) and octopus rhodopsin (lambda max = 475 nm). Both pigments have 11-cis-retinal (lambda max = 379 nm, in ethanol) as their chromophore. These rhodopsins were bleached in their native membranes, and the opsins ...

journal_title：Biochemistry

authors： Koutalos Y,Ebrey TG,Tsuda M,Odashima K,Lien T,Park MH,Shimizu N,Derguini F,Nakanishi K,Gilson HR

更新日期：1989-03-21 00:00:00

abstract：:Annexin V (AxV) is a member of a family of proteins that exhibit functionally relevant Ca2+-dependent binding to anionic phospholipid membranes. Protein structure and stability as a function of Ca2+ and phospholipids was studied by bulk phase infrared (IR) spectroscopy and by IR reflection-absorption spectroscopy (IRR...

journal_title：Biochemistry

authors： Wu F,Flach CR,Seaton BA,Mealy TR,Mendelsohn R

更新日期：1999-01-12 00:00:00

abstract：:In an irreversible enzyme-catalyzed reaction, strong binding of the products would lead to substantial product inhibition. The X-ray crystal structure of the product complex of thymidylate synthase (1.83-A resolution, R factor = 0.183 for all data between 7.0 and 1.83 A) identifies a bound water molecule that serves t...

journal_title：Biochemistry

authors： Fauman EB,Rutenber EE,Maley GF,Maley F,Stroud RM

更新日期：1994-02-15 00:00:00

abstract：:Maduropeptin (MDP) is a recently isolated antitumor antibiotic, consisting of an enediyne-containing chromophore embedded in a highly acidic protein. This holoantibiotic damages duplex DNA in vitro, producing a mixture of single- and double-strand breaks at selected sites. The chromophore, isolated as the methanol add...

journal_title：Biochemistry

authors： Zein N,Solomon W,Colson KL,Schroeder DR

更新日期：1995-09-12 00:00:00

abstract：:Ca2+-activated Z-disk-removing activity in the P0-40 crude muscle extracts described by Busch et al. (Busch, W. A., Stromer, M. H., Goll, D. E., and Suzuki, A. (1972), J. Cell Biol. 52, 367) was purified from porcine skeletal muscle extracts by using five column chromatographic procedures in succession: (1) 6% agarose...

journal_title：Biochemistry

authors： Dayton WR,Goll DE,Zeece MG,Robson RM,Reville WJ

更新日期：1976-05-18 00:00:00

abstract：:The chemical mechanism of phosphodiester bond hydrolysis catalyzed by a junction-resolving enzyme has been investigated. Endonuclease I of phage T7 is a member of the nuclease superfamily of proteins that include many restriction enzymes, and the structure of the active site is very similar to that of BglI in particul...

journal_title：Biochemistry

authors： Liu J,Déclais AC,Lilley DM

更新日期：2006-03-28 00:00:00

abstract：:As discussed in the accompanying paper [Markland, W., Ley, A. C., & Ladner, R. C. (1996) Biochemistry 35, 8045-8057], we generated libraries from the first Kunitz domain of human lipoprotein-associated coagulation inhibitor (LACI-D1) using multivalent M13 III display and derived potent inhibitors of human plasmin (PLA...

journal_title：Biochemistry

authors： Markland W,Ley AC,Ladner RC

更新日期：1996-06-18 00:00:00

abstract：:A mutant form of the nitrogenase iron protein with a deletion of residue Leu 127, located in the switch II region of the nucleotide binding site, forms a tight, inactive complex with the nitrogenase molybdenum iron (MoFe) protein in the absence of nucleotide. The structure of this complex generated with proteins from ...

journal_title：Biochemistry

authors： Chiu H,Peters JW,Lanzilotta WN,Ryle MJ,Seefeldt LC,Howard JB,Rees DC

更新日期：2001-01-23 00:00:00

abstract：:The luminescence of Cu-substituted cytochrome c exhibits temperature-dependent decay modes and spectral shifts at temperatures below 80 K. By comparison with the data from metal-substituted porphyrins, we determined intramolecular rate parameters. The observed tripdoublet decay time is 13 +/- 1 mus at 77 K and the qua...

journal_title：Biochemistry

authors： Glatz P,Chance B,Vanderkooi JM

更新日期：1979-08-07 00:00:00

abstract：:A short chain dehydrogenase like l-threonine 3-dehydrogenase (SDR-TDH) from metagenome data (mtTDH) was identified by database mining. Its enzymatic properties suggested that mtTDH has unique characteristics relative to other SDR-TDHs, including two mesophilic and thermophilic SDR-TDHs identified in this study. The ac...

journal_title：Biochemistry

authors： Motoyama T,Nakano S,Yamamoto Y,Tokiwa H,Asano Y,Ito S

更新日期：2017-10-31 00:00:00

abstract：:Primase is an essential DNA replication enzyme in Escherichia coli and responsible for primer synthesis during lagging strand DNA replication. Although the interaction of primase with single-stranded DNA plays an important role in primer RNA and Okazaki fragment synthesis, the mechanism of DNA binding and site selecti...

journal_title：Biochemistry

authors： Khopde S,Biswas EE,Biswas SB

更新日期：2002-12-17 00:00:00

abstract：:Mammalian cGMP- and cAMP-dependent protein kinase show considerable similarity in amino acid sequence, although they specifically bind different cyclic nucleotides. Results of cGMP analogue binding experiments, combined with modeling of the cGMP binding sites by analogy to the structure of the homologous catabolite ge...

journal_title：Biochemistry

authors： Weber IT,Shabb JB,Corbin JD

更新日期：1989-07-11 00:00:00

abstract：:The infectious isoform of the prion protein (PrPSc) is derived from cellular PrP (PrPC) in a conversion reaction involving a dramatic reorganization of secondary and tertiary structure. While our understanding of the pathogenic role of PrPSc has grown, the normal physiologic function of PrPC still remains unclear. Usi...

journal_title：Biochemistry

authors： Stöckel J,Safar J,Wallace AC,Cohen FE,Prusiner SB

更新日期：1998-05-19 00:00:00

abstract：:To reduce ribonucleotides to deoxyribonucleotides, the manganese-bound form of class Ib ribonucleotide reductase (RNR) must be activated via a pathway that involves redox protein(s). The reduced flavoprotein NrdI is an important protein in this pathway, as it reduces dioxygen to superoxide. Superoxide then reacts with...

journal_title：Biochemistry

authors： Lofstad M,Gudim I,Hammerstad M,Røhr ÅK,Hersleth HP

更新日期：2016-09-13 00:00:00

abstract：:We have previously shown that the anticodon of methionine tRNAs contains most, if not all, of the nucleotides required for specific recognition of tRNA substrates by Escherichia coli methionyl-tRNA synthetase [Schulman, L. H., & Pelka, H. (1988) Science 242, 765-768]. Previous cross-linking experiments have also ident...

journal_title：Biochemistry

authors： Ghosh G,Pelka H,Schulman LH

更新日期：1990-03-06 00:00:00

abstract：:To elucidate a unique mechanism for the quinol oxidation in the Escherichia coli cytochrome bo, we applied pulse radiolysis technique to the wild-type enzyme with or without a single bound ubiquinone-8 at the high-affinity quinone binding site (Q(H)), using N-methylnicotinamide (NMA) as an electron mediator. With the ...

journal_title：Biochemistry

authors： Kobayashi K,Tagawa S,Mogi T

更新日期：2000-12-19 00:00:00

abstract：:We have studied a group of fusion peptides of influenza hemagglutinin in which the N-terminal amino acid, Gly (found in the wild-type peptide), has been systematically substituted with Ala, Ser, Val, or Glu. The activity of the intact hemagglutinin protein with these same substitutions has already been reported. As a ...

journal_title：Biochemistry

authors： Epand RM,Epand RF,Martin I,Ruysschaert JM

更新日期：2001-07-31 00:00:00

abstract：:The structural basis for the improvement in catalytic efficiency of the mutant E165D chicken triosephosphate isomerase by the secondary mutation, S96P, has been analyzed using a combination of X-ray crystallography and Fourier transform infrared spectroscopy. All X-ray structures were of the complex of phosphoglycoloh...

journal_title：Biochemistry

authors： Komives EA,Lougheed JC,Liu K,Sugio S,Zhang Z,Petsko GA,Ringe D

更新日期：1995-10-17 00:00:00

abstract：:Ultraviolet thermal denaturation studies substantiate our earlier hypothesis that substitution of a L-nucleotide residue for a D-nucleotide within a DNA duplex permits a stable structure in which all bases are paired through Watson-Crick hydrogen bonds (Damha, M. J., Giannaris, P. A., Marfey, P., & Reid, L. S. (1991) ...

journal_title：Biochemistry

authors： Damha MJ,Giannaris PA,Marfey P

更新日期：1994-06-28 00:00:00

abstract：:Cyanide inhibits the CO oxidation activity of carbon monoxide dehydrogenase from Clostridium thermoaceticum by binding tightly to the form of the C-cluster yielding the gav = 1.82 signal (the C1.82 form). CN- dissociates and the enzyme reactivates upon addition of CO, CO2 plus dithionite, or CS2 plus dithionite. Dithi...

journal_title：Biochemistry

authors： Anderson ME,Lindahl PA

更新日期：1994-07-26 00:00:00

abstract：:cis peptide bonds in proteins are often rate-limiting steps in protein folding or conformational change and are frequently stabilized by metal ions. In the collagen-binding domain of Clostridium histolyticum collagenase, the binding of calcium ions triggers the formation of a cis peptide bond. We present free energy s...

journal_title：Biochemistry

authors： Spiriti J,van der Vaart A

更新日期：2010-06-29 00:00:00