Predicted structures of the cGMP binding domains of the cGMP-dependent protein kinase: a key alanine/threonine difference in evolutionary divergence of cAMP and cGMP binding sites.

abstract：:To elucidate a unique mechanism for the quinol oxidation in the Escherichia coli cytochrome bo, we applied pulse radiolysis technique to the wild-type enzyme with or without a single bound ubiquinone-8 at the high-affinity quinone binding site (Q(H)), using N-methylnicotinamide (NMA) as an electron mediator. With the ...

journal_title：Biochemistry

authors： Kobayashi K,Tagawa S,Mogi T

更新日期：2000-12-19 00:00:00

abstract：:Quantum tunneling and protein dynamics have emerged as important components of enzyme function. This review focuses on soybean lipoxygenase-1, to illustrate how the properties of enzymatic C-H bond activation link protein motions to the fundamental bond making-breaking processes. ...

journal_title：Biochemistry

authors： Klinman JP

更新日期：2013-03-26 00:00:00

abstract：:End binding protein 1 (EB1) and cytoplasmic linker protein of 170 kDa (CLIP-170) are two well-studied microtubule plus-end-tracking proteins (+TIPs) that target growing microtubule plus ends in the form of comet tails and regulate microtubule dynamics. However, the mechanism by which they regulate microtubule dynamics...

journal_title：Biochemistry

authors： Lopus M,Manatschal C,Buey RM,Bjelić S,Miller HP,Steinmetz MO,Wilson L

更新日期：2012-04-10 00:00:00

abstract：:Isotope effects have been measured with Mg2+ as the activator and L-malate labeled with deuterium or tritium at carbon 2 as the substrate over the pH range 4-10. Comparison of the nearly pH-independent deuterium-isotope effect on V/Kmalate of 1.5 with the tritium effect of 2.0 by the method of Northrop (Northrop, D.B....

journal_title：Biochemistry

authors： Schimerlik MI,Grimshaw CE,Cleland WW

更新日期：1977-02-22 00:00:00

abstract：:A covalently linked actin dimer is identified in solutions of actin prepared from an acetone powder from skeletal muscle. This actin dimer acts as an actin nucleating factor (ANF), decreasing the half-time for spontaneous actin polymerization. ANF reacts with antibodies to both the N- and C-terminal portions of actin ...

journal_title：Biochemistry

authors： Selden LA,Kinosian HJ,Estes JE,Gershman LC

更新日期：2000-01-11 00:00:00

abstract：:The contact between EcoRI endonuclease and the "primary clamp" phosphate of its recognition site pGAATTC is absolutely required for recognition of the canonical and all variant DNA sites. We have probed this contact using oligonucleotides containing single stereospecific (Rp)- or (Sp)- phosphorothioates (Ps). At the G...

journal_title：Biochemistry

authors： Kurpiewski MR,Koziolkiewicz M,Wilk A,Stec WJ,Jen-Jacobson L

更新日期：1996-07-09 00:00:00

abstract：:An RNA that replicates with core RNA polymerase from E. coli and the substrates ATP, CTP, ITP, and UTP, was selected from a random poly(A,U,I,C) library and named EcorpI. Another replicating RNA, EcorpG, was obtained by template-free incubation of holo RNA polymerase and the substrates ATP, CTP, GTP, and UTP. Both RNA...

journal_title：Biochemistry

authors： Wettich A,Biebricher CK

更新日期：2001-03-20 00:00:00

abstract：:Urease from Klebsiella aerogenes [Jabri et al. (1995) Science 268, 998-1004] is an (alpha beta gamma)3 trimer with each alpha-subunit having an (alpha beta)8-barrel domain containing a binickel active center. Here we examine structure-function relations for urease in more detail through structural analysis of the urea...

journal_title：Biochemistry

authors： Jabri E,Karplus PA

更新日期：1996-08-20 00:00:00

abstract：:By limited proteolysis of a type kappa immunoglobulin light chain (Oku) with clostripain, both the VL and CL fragments were obtained with a high yield. The unfolding and refolding by guanidine hydrochloride of light chain Oku and its VL and CL fragments were studied at pH 7.5 and 25 degrees C with circular dichroism a...

journal_title：Biochemistry

authors： Tsunenaga M,Goto Y,Kawata Y,Hamaguchi K

更新日期：1987-09-22 00:00:00

abstract：:DNA (cytosine-5-)-methyltransferase is essential for viable mammalian development and has a central function in the determination and maintenance of epigenetic methylation patterns. Steady-state and substrate trapping studies were performed to better understand how the enzyme functions. The catalytic efficiency was de...

journal_title：Biochemistry

authors： Flynn J,Reich N

更新日期：1998-10-27 00:00:00

abstract：:Dynamic light scattering measurements were carried out on secretin in aqueous solution (2 mM; pH 5.0). The results indicated that the molecule exists as a fairly compact hexamer under these solution conditions. Secondary structural properties of the secretin hexameric complex were evaluated using CD and NMR spectrosco...

journal_title：Biochemistry

authors： Carpenter KA,Schiller PW

更新日期：1998-12-01 00:00:00

abstract：:The C-terminal domain (CT) of apolipoprotein E (apoE), a critical protein involved in cholesterol transport in the plasma and brain, plays an important role in high-affinity lipoprotein binding. Although high-resolution structural information is available for the N-terminal domain of apoE, the structural organization ...

journal_title：Biochemistry

authors： Patel AB,Khumsupan P,Narayanaswami V

更新日期：2010-03-02 00:00:00

abstract：:1H NMR studies on the nonselective endothelin receptor agonist sarafotoxin SRTb have identified a helix between residues Asp 8 and His 16, and a beta-turn involving residues Cys 3 to Met 6; however, the biologically important C-terminal five residues were found to be conformationally variable. The average RMSD, measur...

journal_title：Biochemistry

authors： Atkins AR,Martin RC,Smith R

更新日期：1995-02-14 00:00:00

abstract：:Herpesvirus saimiri codes for a tyrosine kinase interacting protein (Tip) that interacts with both the SH3 domain and the kinase domain of the T-cell-specific tyrosine kinase Lck via two separate motifs. The activation of Lck by Tip is considered as a key event in the transformation of human T-lymphocytes during herpe...

journal_title：Biochemistry

authors： Schweimer K,Hoffmann S,Bauer F,Friedrich U,Kardinal C,Feller SM,Biesinger B,Sticht H

更新日期：2002-04-23 00:00:00

abstract：:The specific deamidation of asparagine-71 of triosephosphate isomerase increases upon substrate binding and catalysis. This deamidation at the dimer interface initiates subunit dissociation, unfolding, and protein degradation. The apparent connection between catalysis and terminal marking supports the concept of "mole...

journal_title：Biochemistry

authors： Garza-Ramos G,Tuena de Gomez-Puyou M,Gomez-Puyou A,Yüksel KU,Gracy RW

更新日期：1994-06-07 00:00:00

abstract：:Thioredoxin reductase from Escherichia coli is a member of the pyridine nucleotide-disulfide oxidoreductase family, and contains one FAD and one redox-active disulfide per subunit. It is known that two other well-studied members of this family, lipoamide dehydrogenase and glutathione reductase, cycle between the two e...

journal_title：Biochemistry

authors： Lennon BW,Williams CH Jr

更新日期：1996-04-16 00:00:00

abstract：:One way to improve the economic feasibility of biomass conversion is to enhance the catalytic efficiency of cellulases through protein engineering. This requires that high-resolution structures of cellulases be available. Here we present the structure of E2cd, the catalytic domain of the thermophilic endocellulase E2 ...

journal_title：Biochemistry

authors： Spezio M,Wilson DB,Karplus PA

更新日期：1993-09-28 00:00:00

abstract：:The structure of empty capsids of Kilham rat virus, an autonomous parvovirus with icosahedral symmetry, was investigated by small-angle neutron scattering. From the forward scatter, the molecular weight was determined to be 4.0 X 10(6), and from the Guinier region, the radius of gyration was found to be 105 A in D2O a...

journal_title：Biochemistry

authors： Wobbe CR,Mitra S,Ramakrishnan V

更新日期：1984-12-18 00:00:00

abstract：:A set of peptides derived from the N-terminal domain of the ribosomal protein L9 (NTL9) have been characterized in an effort to define the minimum unit of this domain required to fold and to provide model peptides for the analysis of electrostatic interactions in the unfolded state. NTL9 is a 56-residue alpha-beta pro...

journal_title：Biochemistry

authors： Horng JC,Moroz V,Rigotti DJ,Fairman R,Raleigh DP

更新日期：2002-11-12 00:00:00

abstract：:In an irreversible enzyme-catalyzed reaction, strong binding of the products would lead to substantial product inhibition. The X-ray crystal structure of the product complex of thymidylate synthase (1.83-A resolution, R factor = 0.183 for all data between 7.0 and 1.83 A) identifies a bound water molecule that serves t...

journal_title：Biochemistry

authors： Fauman EB,Rutenber EE,Maley GF,Maley F,Stroud RM

更新日期：1994-02-15 00:00:00

abstract：:During DNA replication, the presence of oligonucleotides with partial homology to the template strand was shown to induce a switch of the polymerase from the normal template to the oligonucleotide. The latter acted as a dead-end template and led to abortive replication. The only prerequisite was that the oligonucleoti...

journal_title：Biochemistry

authors： Guieysse AL,Praseuth D,Grigoriev M,Harel-Bellan A,Hélène C

更新日期：1995-07-18 00:00:00

abstract：:TCDD (dioxin) induces a rapid inflammatory response from 3T3-L1 adipocytes as judged by prominent induction of the mRNA expression of prostaglandin-endperoxide synthase 2 (Cox-2) along with other inflammation markers within 1 h. This action of TCDD is clearly antagonized by cell pretreatment with AACOCF3 (an inhibitor...

journal_title：Biochemistry

authors： Li W,Matsumura F

更新日期：2008-12-30 00:00:00

abstract：:Direct chemical titrations of reduced, purified cytochrome aa3 were monitored at 604 nm. Anaerobic oxidation by potassium ferricyanide or 1,1'-bis(hydroxymethyl)-ferricinium ion was compared with the natural substrate, molecular oxygen. The four electrons from reduced cytochrome aa3 were donated to one oxygen molecule...

journal_title：Biochemistry

authors： Schroedl NA,Hartzell CR

更新日期：1977-04-05 00:00:00

abstract：:Phosphorescence from the lone tryptophan residue has been studied to monitor the interaction of myelin basic protein with phosphatidylserine vesicles. Spectral shifts in the phosphorescence of the protein in a glycerol-buffer (70:30 w/w) solvent at low temperature are consistent with fluorescence data obtained under a...

journal_title：Biochemistry

authors： Vadas EB,Melançon P,Braun PE,Galley WC

更新日期：1981-05-26 00:00:00

abstract：:Hepatic microsomal NADPH-cytochrome P-450 reductase was solubilized from rabbit liver microsomes in the presence of detergents and purified to homogeneity by column chromatography. The purified reductase had a molecular weight of 78 000 and contained 1 mol each of FAD and FMN per mol of enzyme. On reduction with NADPH...

journal_title：Biochemistry

authors： Iyanagi T,Anan FK,Imai Y,Mason HS

更新日期：1978-05-30 00:00:00

abstract：:Adenylosuccinate lyase of Bacillus subtilis is inactivated by 2-[(4-bromo-2,3-dioxobutyl)thio]adenosine 5'-monophosphate (2-BDB-TAMP) at pH 7.0. As the reagent concentration is increased, a maximum rate constant is approached, indicative of reversible enzyme-reagent complex formation (KR = 68 +/- 9 microM) prior to ir...

journal_title：Biochemistry

authors： Lee TT,Worby C,Bao ZQ,Dixon JE,Colman RF

更新日期：1998-06-09 00:00:00

abstract：:Hydrogen-bonding and ionic interactions play fundamental roles in macromolecular recognition and function. In contrast to lysines and arginines, how histidines mediate these interactions is less well-understood due to the unique properties of its side chain imidazole that include an aromatic ring with two titratable n...

journal_title：Biochemistry

authors： Sepuru KM,Rajarathnam K

更新日期：2018-10-16 00:00:00

abstract：:Preferential interaction of trans-membrane alpha-helices whose hydrophobic length matches the hydrophobic thickness of the lipid bilayer could be a mechanism of retention in the Golgi apparatus. We have used fluorescence methods to study the interaction of peptides Ac-K2-G-Lm-W-Ln-K2-A-amide (Pm+n) with bilayers of ph...

journal_title：Biochemistry

authors： Webb RJ,East JM,Sharma RP,Lee AG

更新日期：1998-01-13 00:00:00

abstract：:By use of two-dimensional NMR techniques most of the proton resonances (greater than 90%) are assigned for the tryptic digest fragment 96-133 of bovine somatotropin in 30% 2,2,2-trifluoroethanol-d3/70% H2O. Qualitative analysis of the nuclear Overhauser enhancement (NOE) data indicates that a region of alpha-helix spa...

journal_title：Biochemistry

authors： Gooley PR,MacKenzie NE

更新日期：1988-05-31 00:00:00

abstract：:The behavior of fluorescent and spin-label probes is examined in several fluid and gel phospholipid phases, with particular focus on the Ca2+-induced gel phase in phosphatidylserine (PS). These probes have behavior characteristic of the type of probe and of the type of lipid environment. Anthroyloxy- and doxyl-labeled...

journal_title：Biochemistry

authors： Florine KI,Feigenson GW

更新日期：1987-03-24 00:00:00