Abstract：
:During DNA replication, the presence of oligonucleotides with partial homology to the template strand was shown to induce a switch of the polymerase from the normal template to the oligonucleotide. The latter acted as a dead-end template and led to abortive replication. The only prerequisite was that the oligonucleotide could form 7-9 base pairs with the newly synthesized DNA strand in order to switch templates. The switch occurred when base pairing of the oligonucleotide could take place with the 3'-end of the newly synthesized strand. These results show that oligonucleotides used in antisense or antigene strategies could have unexpected effects on replication. In addition, oligonucleotide-directed abortive replication might play an inhibitory role during PCR experiments on long DNA templates and lead to the amplification of truncated fragments.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Guieysse AL,Praseuth D,Grigoriev M,Harel-Bellan A,Hélène Cdoi
10.1021/bi00028a032subject
Has Abstractpub_date
1995-07-18 00:00:00pages
9193-9issue
28eissn
0006-2960issn
1520-4995journal_volume
34pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Heparanase is a beta-D-endoglucuronidase that cleaves heparan sulfate (HS) and has been implicated in many important physiological and pathological processes, including tumor cell metastasis, angiogenesis, and leukocyte migration. We report herein the identification of active-site residues of human heparanase. Using P...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002080p
更新日期：2000-12-26 00:00:00
abstract：:Benzo[c]phenanthrene diol epoxide can covalently bind to the exocyclic amino group of deoxyadenosine to generate [BPh]dA adducts where the polycyclic aromatic hydrocarbon is attached to the major groove edge of DNA. This paper reports on NMR-energy minimization structural studies of the (+)-trans-anti-[BPh]dA adduct p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00097a029
更新日期：1993-11-23 00:00:00
abstract：:Shaker potassium (K+) channels normally lack intrasubunit and intersubunit disulfide bonds. However, disulfide bonds are formed between Shaker subunits in intact cells exposed to oxidizing conditions. Upon electrophoresis under nonreducing conditions, intersubunit disulfide bond formation was detected by the presence ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961083s
更新日期：1996-09-17 00:00:00
abstract：:This paper contributes to the characterization of partial steps of electron and proton transfer in mitochondrial cytochrome c oxidase with respect to their membrane arrangement and involvement in energy-linked protonmotive activity. It is shown that delta psi controls electron flow from cytochrome c to heme a is consi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00464a008
更新日期：1990-03-27 00:00:00
abstract：:Amyloid fibrils have been classically defined as linear, nonbranched polymeric proteins with a cross beta-sheet structure and the ability to alter the optical properties of the amyloid-specific dye Congo Red. Mounting evidence suggests that soluble oligomeric peptide assemblies approximately 2-20 nm in diameter are cr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700247y
更新日期：2007-06-19 00:00:00
abstract：:The 1.9 A X-ray crystal structure of human myeloperoxidase complexed with cyanide (R = 0.175, R(free) = 0.215) indicates that cyanide binds to the heme iron with a bent Fe-C-N angle of approximately 157 degrees, and binding is accompanied by movement of the iron atom by 0.2 A into the porphyrin plane. The bent orienta...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0111808
更新日期：2001-11-20 00:00:00
abstract：:Aspartate-beta-semialdehyde dehydrogenase (ASADH) lies at the first branch point in the biosynthetic pathway through which bacteria, fungi, and the higher plants synthesize amino acids, including lysine and methionine and the cell wall component diaminopimelate from aspartate. Blocks in this biosynthetic pathway, whic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015713o
更新日期：2001-12-04 00:00:00
abstract：:Integrin αIIbβ3, a transmembrane heterodimer, mediates platelet aggregation when it switches from an inactive to an active ligand-binding conformation following platelet stimulation. Central to regulating αIIbβ3 activity is the interaction between the αIIb and β3 extracellular stalks, which form a tight heterodimer in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00430
更新日期：2019-07-30 00:00:00
abstract：:The contributions to thermostability of interactions within the beta-sheet region of rubredoxins (Rds) were investigated by examining proteins in which beta-strand sequences of Rds from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) and the mesophilic bacterium Clostridium pasteurianum (Cp) were interchanged....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970110r
更新日期：1997-08-26 00:00:00
abstract：:Piscidin 1 (Pis-1) is a novel cytotoxic peptide with a cationic alpha-helical structure that was isolated from the mast cells of hybrid striped bass [Silphaduang, U., and Noga, E. J. (2001) Nature 414, 268-269]. Pis-1 is not selective for bacterial versus mammalian cells. In the present study, to develop novel antibio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi062233u
更新日期：2007-03-27 00:00:00
abstract：:Kinetic study of a series of compounds containing the thrombin-directed peptide D-Phe-ProboroArg-OH had indicated that the structure of the N-terminal blocking group may be correlated with binding [Kettner, C., Mersinger, L., & Knabb, R. (1990) J. Biol. Chem. 265, 18289-18297]. In order to further study this phenomeno...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952164b
更新日期：1996-07-30 00:00:00
abstract：:A slow conversion of adenine residues to hypoxanthine occurs in single-stranded DNA when heated in neutral aqueous buffers. The rate of this reaction at pH 7.6 and 110 degrees C is k = 4 x 10(-8) s-1, as determined by base analysis of heat-treated DNA that contains radioactively labeled adenine residues. It is propose...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00567a010
更新日期：1980-12-23 00:00:00
abstract：:Staphylococcus aureus transcription factor QacR regulates expression of the qacA multidrug efflux determinant. In response to binding cationic lipophilic compounds, including ethidium and rhodamine 6G, QacR dissociates from the qacA operator alleviating repression. Such ligand binding uniformly induces a coil-to-helix...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901102h
更新日期：2009-10-20 00:00:00
abstract：:The relative activities of 313 mutants of the gene V protein of bacteriophage f1, assayed in vivo, have been used to evaluate two approaches to predicting the effects of single amino acid substitutions on the function of a protein. First, we tested methods that only depend on the properties of the wild-type and substi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00239a022
更新日期：1991-06-25 00:00:00
abstract：:The C-terminal domain from the muscle protein troponin C (TnC) comprises two helix-loop-helix calcium-binding sites (residues 90-162). The assembly of these two sites is governed by calcium binding enabling a synthetic C-terminal domain to be preferentially and stoichiometrically assembled from two synthetic peptides ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9528006
更新日期：1996-06-11 00:00:00
abstract：:The mammalian plasma vitamin D binding protein (DBP), or Gc-globulin, is recognized to have at least two functional properties: sterol binding and G-actin sequestration. Affinity labeling of the sterol binding site with the radioactive electrophilic ligand, 3 beta-(bromoacetoxy)-25-hydroxycholecalciferol, followed by ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00146a021
更新日期：1992-08-11 00:00:00
abstract：:While calcium binding to troponin C (TnC) triggers the contraction of both skeletal and cardiac muscle, there is clear evidence that different mechanisms may be involved. For example, activation of heart myofilaments occurs with binding to a single regulatory site on TnC, whereas activation of fast skeletal myofilamen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971223d
更新日期：1997-10-07 00:00:00
abstract：:DnaB helicase is responsible for unwinding duplex DNA during chromosomal DNA replication and is an essential component of the DNA replication apparatus in Escherichia coli. We have analyzed the mechanism of binding of single-stranded DNA (ssDNA) by the DnaB x DnaC complex and DnaB helicase. Binding of ssDNA to DnaB he...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060118d
更新日期：2006-09-26 00:00:00
abstract：:The expression level of protein DR1199 is observed to increase considerably in the radio-resistant bacterium Deinococcus radiodurans following irradiation. This protein belongs to the DJ-1 superfamily, which includes proteins with diverse functions, such as the archaeal proteases PhpI and PfpI, the bacterial chaperone...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800882v
更新日期：2008-11-04 00:00:00
abstract：:The interactions of Alzheimer's amyloid beta-peptide with cyclodextrins were studied by (1)H NMR: the translational diffusion coefficient of the peptide and chemical shift changes were studied by the presence of variable concentrations of cyclodextrins. For the full-length peptide, Abeta(1-40), the combined results of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi036254p
更新日期：2004-05-25 00:00:00
abstract：:One of the arguments in favor of the protein-only hypothesis of transmissible spongiform encephalopathies is the link between inherited prion diseases and specific mutations in the PRNP gene. One such mutation (Asp178 --> Asn) is associated with two distinct disorders: fatal familial insomnia or familial Creutzfeldt-J...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051455+
更新日期：2005-12-06 00:00:00
abstract：:The chirality of sulfoxidation catalyzed by two cytochrome P-450 isozymes purified from phenobarbital-induced rat liver was studied by using 4-tolyl ethyl sulfide as a substrate. Both P-450 isoenzymes, termed PB-1 and PB-4, when reconstituted with purified rat liver NADPH-cytochrome P-450 reductase and cytochrome b5, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00539a032
更新日期：1982-05-11 00:00:00
abstract：:The mitogen-activated protein kinases (MAPKs) are key components of cellular signal transduction pathways, which are down-regulated by the MAPK phosphatases (MKPs). Catalytic activity of the MKPs is controlled both by their ability to recognize selective MAPKs and by allosteric activation upon binding to MAPK substrat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00827
更新日期：2017-11-21 00:00:00
abstract：:Scaffold/matrix-attached regions (S/MARs) are cis-acting elements with a function outside transcribed regions and in introns. Although they usually augment transcriptional rates, their action is highly context-dependent. We cloned an 800 bp S/MAR element from the upstream border of the human interferon-beta domain at ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960930o
更新日期：1996-08-27 00:00:00
abstract：:The structural domains of human apolipoprotein(a) [apo(a)] and its interaction with apolipoprotein B-100 (apo B-100) in the lipoprotein(a) [Lp(a)] particle were investigated by limited proteolysis with thermolysin and cathepsin D. We characterized the proteolytic products by sodium dodecyl sulfate-polyacrylamide gradi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00177a026
更新日期：1994-03-22 00:00:00
abstract：:The thermodynamic binding parameters and crystal structure for streptavidin-peptide complexes where the peptide sequences were obtained by random screening methods are reported. The affinities between streptavidin and two heptapeptides were determined by titrating calorimetric methods [Phe-Ser-His-Pro-Gln-Asn-Thr, Ka ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00154a004
更新日期：1992-10-06 00:00:00
abstract：:Deposition of amyloid beta-peptide (Abeta) into amyloid plaques is one of the invariant neuropathological features of Alzheimer's disease. Proteins that codeposit with Abeta are potentially important for the pathogenesis, and a recently discovered plaque-associated protein is the collagenous Alzheimer amyloid plaque c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051263e
更新日期：2005-11-29 00:00:00
abstract：:Hydrophobic interactions are responsible for stabilizing leucine zippers in peptides containing heptad repeats. The effects of substituting leucine by phenylalanine and alanine by glycine on the self-assembly of coiled-coils were examined in minimalist antimicrobial peptides designed to form amphipathic alpha-helices....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961644f
更新日期：1997-08-05 00:00:00
abstract：:Human alpha1 and alpha2 isoforms of Na,K-ATPase have been expressed with porcine 10*Histidine-tagged beta1 subunit in Pichia pastoris. Methanol-induced expression of alpha2 is optimal at 20 degrees C, whereas at 25 degrees C, which is optimal for expression of alpha1, alpha2 is not expressed. Detergent-soluble alpha2b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701812c
更新日期：2007-12-25 00:00:00
abstract：:Changes in the concentrations of high-energy phosphate metabolites were measured by 31P NMR spectroscopy of surviving rat uteri from 0-48 h following estrogen administration. Concentrations (millimoles per kilogram wet weight) of these metabolites in the untreated immature uterus, measured at 4 degrees C, were found t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00307a006
更新日期：1984-06-05 00:00:00
