Human myeloperoxidase: structure of a cyanide complex and its interaction with bromide and thiocyanate substrates at 1.9 A resolution.

Abstract:

:The 1.9 A X-ray crystal structure of human myeloperoxidase complexed with cyanide (R = 0.175, R(free) = 0.215) indicates that cyanide binds to the heme iron with a bent Fe-C-N angle of approximately 157 degrees, and binding is accompanied by movement of the iron atom by 0.2 A into the porphyrin plane. The bent orientation of the cyanide allows the formation of three hydrogen bonds between its nitrogen atom and the distal histidine as well as two water molecules in the distal cavity. The 1.85 A X-ray crystal structure of an inhibitory complex with thiocyanate (R = 0.178, R(free) = 0.210) indicates replacement of chloride at a proximal helix halide binding site in addition to binding in the distal cavity in an orientation parallel with the heme. The thiocyanate replaces two water molecules in the distal cavity and is hydrogen bonded to Gln 91. The 1.9 A structures of the complexes formed by bromide (R = 0.215, R(free) = 0.270) and thiocyanate (R = 0.198, R(free) = 0.224) with the cyanide complex of myeloperoxidase show how the presence of bound cyanide alters the binding site for bromide in the distal heme cavity, while having little effect on thiocyanate binding. These results support a model for a single common binding site for halides and thiocyanate as substrates or as inhibitors near the delta-meso carbon of the porphyrin ring in myeloperoxidase.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Blair-Johnson M,Fiedler T,Fenna R

doi

10.1021/bi0111808

subject

Has Abstract

pub_date

2001-11-20 00:00:00

pages

13990-7

issue

46

eissn

0006-2960

issn

1520-4995

pii

bi0111808

journal_volume

40

pub_type

杂志文章
  • Interaction of mant-adenosine nucleotides and magnesium with kinesin.

    abstract::Displacement of the fluorescent substrate analogue methylanthraniloyl ADP (mant-ADP) from kinesin by excess ATP results in a biphasic fluorescent transient. The pH and microtubule dependence of the rates and amplitudes indicates that the two phases are produced by release of bound mant-ADP, with an excess of the 3'-is...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi972742j

    authors: Cheng JQ,Jiang W,Hackney DD

    更新日期:1998-04-14 00:00:00

  • Activation of cGMP phosphodiesterase in retinal rods: mechanism of interaction with the GTP-binding protein (transducin).

    abstract::The mechanism of activation of cGMP phosphodiesterase by the GTP-binding protein in the disc membrane of retinal rods has been investigated by measuring the light-induced phosphodiesterase activity in reconstituted systems where the concentration of either the GTP-binding protein or the phosphodiesterase is varied. Th...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00444a040

    authors: Bennett N,Clerc A

    更新日期:1989-09-05 00:00:00

  • Modulation of MutS ATP hydrolysis by DNA cofactors.

    abstract::Escherichia coli MutS protein, which is required for mismatch repair, has a slow ATPase activity that obeys Michalelis-Menten kinetics. At 37 degrees C, the steady-state turnover rate for ATP hydrolysis is 1.0 +/- 0.3 min(-1) per monomer equivalent with a K(m) of 33 +/- 6 microM. Hydrolysis is competitively inhibited ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi992286u

    authors: Bjornson KP,Allen DJ,Modrich P

    更新日期:2000-03-21 00:00:00

  • Role of magnesium binding to myosin in controlling the state of cross-bridges in skeletal rabbit muscle.

    abstract::The effect of Mg2+ on the disposition of myosin cross-bridges was studied on myofibrils and synthetic myosin and rod filaments by employing chymotryptic digestion and chemical cross-linking methods. In the presence of low Mg2+ concentrations (0.1 mM), the proteolytic susceptibility at the heavy meromyosin/light meromy...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00290a012

    authors: Reisler E,Liu J,Cheung P

    更新日期:1983-10-11 00:00:00

  • Additive transfer free energies of the peptide backbone unit that are independent of the model compound and the choice of concentration scale.

    abstract::With knowledge of individual transfer free energies of chemical groups that become newly exposed on protein denaturation and assuming the group transfer free energy contributions are additive, it should be possible to predict the stability of a protein in the presence of denaturant. Unfortunately, several unresolved i...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi035908r

    authors: Auton M,Bolen DW

    更新日期:2004-02-10 00:00:00

  • Common chelatase design in the branched tetrapyrrole pathways of heme and anaerobic cobalamin synthesis.

    abstract::Prosthetic groups such as heme, chlorophyll, and cobalamin (vitamin B(12)) are characterized by their branched biosynthetic pathway and unique metal insertion steps. The metal ion chelatases can be broadly classed either as single-subunit ATP-independent enzymes, such as the anaerobic cobalt chelatase and the protopor...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9906773

    authors: Schubert HL,Raux E,Wilson KS,Warren MJ

    更新日期:1999-08-17 00:00:00

  • Cytosolic region of TM6 in P-glycoprotein: topographical analysis and functional perturbation by site directed labeling.

    abstract::Reduced intracellular drug accumulation due to the activity of the drug efflux pump ABC (B1) is a major mechanism in the resistance of cancer cells to chemotherapy. ABC (B1) is a poly specific transporter, and the molecular mechanism of its complex translocation process remains to be elucidated. To understand the proc...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi7023089

    authors: Storm J,Modok S,O'Mara ML,Tieleman DP,Kerr ID,Callaghan R

    更新日期:2008-03-25 00:00:00

  • Picosecond absorption studies on rhodopsin and isorhodopsin in detergent and native membrane.

    abstract::Picosecond transient absorption spectra of rhodopsin and isorhodopsin were measured at room temperature with a double-beam laser spectrophotometer after excitation at 355 nm. Photolysis studies were performed on rhodopsin solubilized in two different detergents (digitonin and 3-[(3-cholamidopropyl)dimethylammonio]-1-p...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00312a008

    authors: Rudzki JE,Peters KS

    更新日期:1984-08-14 00:00:00

  • The hydrogen-bonding structure in parallel-stranded duplex DNA is reverse Watson-Crick.

    abstract::Raman spectra of the parallel-stranded duplex formed from the deoxyoligonucleotides 5'-d-[(A)10TAATTTTAAATATTT]-3' (D1) and 5'-d[(T)10ATTAAAATTTATAAA]-3' (D2) in H2O and D2O have been acquired. The spectra of the parallel-stranded DNA are then compared to the spectra of the antiparallel double helix formed from the de...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00226a012

    authors: Otto C,Thomas GA,Rippe K,Jovin TM,Peticolas WL

    更新日期:1991-03-26 00:00:00

  • Kinetic study of DNA condensation by cationic peptides used in nonviral gene therapy: analogy of DNA condensation to protein folding.

    abstract::Synthetic nonviral vector systems are attractive because of their apparent simplicity of preparation and use. However, there are many barriers to success at the moment, including the formulation of uniform and reproducible particles of transfection competent, condensed nucleic acids such as plasmid DNA (pDNA). For thi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi034325e

    authors: Tecle M,Preuss M,Miller AD

    更新日期:2003-09-09 00:00:00

  • Glutamate 329 located in the fourth transmembrane segment of the alpha-subunit of the rat kidney Na+,K+-ATPase is not an essential residue for active transport of sodium and potassium ions.

    abstract::An allelic variant of the ouabain-insensitive rat kidney Na+,K(+)-ATPase alpha 1-isoform was identified by chance in a cDNA library. The variant differed from the wild-type rat kidney Na+,K(+)-ATPase by a single G to C base substitution in the cDNA, which on the amino acid level gave rise to a glutamine in place of th...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00211a048

    authors: Vilsen B

    更新日期:1993-12-07 00:00:00

  • Reduction of the [2Fe-2S] cluster accompanies formation of the intermediate 9-mercaptodethiobiotin in Escherichia coli biotin synthase.

    abstract::Biotin synthase catalyzes the conversion of dethiobiotin (DTB) to biotin through the oxidative addition of sulfur between two saturated carbon atoms, generating a thiophane ring fused to the existing ureido ring. Biotin synthase is a member of the radical SAM superfamily, composed of enzymes that reductively cleave S-...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi201042r

    authors: Taylor AM,Stoll S,Britt RD,Jarrett JT

    更新日期:2011-09-20 00:00:00

  • Membrane binding kinetics of protein kinase C betaII mediated by the C2 domain.

    abstract::Conventional isoforms of protein kinase C (PKC) are activated when their two membrane-targeting modules, the C1 and C2 domains, bind the second messengers diacylglycerol (DG) and Ca2+, respectively. This study investigates the mechanism of Ca2+-induced binding of PKC betaII to anionic membranes mediated by the C2 doma...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi010761u

    authors: Nalefski EA,Newton AC

    更新日期:2001-11-06 00:00:00

  • Two disaccharides and trimethylamine N-oxide affect Abeta aggregation differently, but all attenuate oligomer-induced membrane permeability.

    abstract::Interaction between aggregates of amyloid beta protein (Abeta) and membranes has been hypothesized by many to be a key event in the mechanism of neurotoxicity associated with Alzheimer's disease (AD). Proposed membrane-related mechanisms of neurotoxicity include ion channel formation, membrane disruption, changes in m...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9006397

    authors: Qi W,Zhang A,Good TA,Fernandez EJ

    更新日期:2009-09-22 00:00:00

  • A Ca2+-activated protease possibly involved in myofibrillar protein turnover. Purification from porcine muscle.

    abstract::Ca2+-activated Z-disk-removing activity in the P0-40 crude muscle extracts described by Busch et al. (Busch, W. A., Stromer, M. H., Goll, D. E., and Suzuki, A. (1972), J. Cell Biol. 52, 367) was purified from porcine skeletal muscle extracts by using five column chromatographic procedures in succession: (1) 6% agarose...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00655a019

    authors: Dayton WR,Goll DE,Zeece MG,Robson RM,Reville WJ

    更新日期:1976-05-18 00:00:00

  • Translocase-bound SecA is largely shielded from the phospholipid acyl chains.

    abstract::Protein translocation in Escherichia coli is mediated by the SecA ATPase bound to the SecYEG membrane protein complex. SecA translocation ATPase activity as well as protein translocation is dependent on the presence of negatively charged lipids. By using a phospholipid with an acyl chain linked photoactivatable group,...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9809021

    authors: van Voorst F,van der Does C,Brunner J,Driessen AJ,de Kruijff B

    更新日期:1998-09-01 00:00:00

  • Inhibition and binding kinetics of the hepatitis C virus NS3 protease inhibitor ITMN-191 reveals tight binding and slow dissociative behavior.

    abstract::The protease activity of hepatitis C virus nonstructural protein 3 (NS3) is essential for viral replication. ITMN-191, a macrocyclic inhibitor of the NS3 protease active site, promotes rapid, multilog viral load reductions in chronic HCV patients. Here, ITMN-191 is shown to be a potent inhibitor of NS3 with a two-step...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi900038p

    authors: Rajagopalan R,Misialek S,Stevens SK,Myszka DG,Brandhuber BJ,Ballard JA,Andrews SW,Seiwert SD,Kossen K

    更新日期:2009-03-24 00:00:00

  • Acyclic guanosine analogs inhibit DNA polymerases alpha, delta, and epsilon with very different potencies and have unique mechanisms of action.

    abstract::Acyclovir triphosphate, ganciclovir triphosphate and penciclovir triphosphate inhibited DNA polymerases alpha, delta, and epsilon. Each triphosphate preferentially inhibited pol delta, although ganciclovir triphosphate was the most impressive of the three; the Ki for inhibition of pol delta was 2 microM (competitive w...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00008a014

    authors: Ilsley DD,Lee SH,Miller WH,Kuchta RD

    更新日期:1995-02-28 00:00:00

  • Two-dimensional NMR studies of staphylococcal nuclease. 1. Sequence-specific assignments of hydrogen-1 signals and solution structure of the nuclease H124L-thymidine 3',5'-bisphosphate-Ca2+ ternary complex.

    abstract::Staphylococcal nuclease H124L is a recombinant protein produced in Escherichia coli whose sequence is identical with that of the nuclease produced by the V8 variant of Staphylococcus aureus. The enzyme-metal ion activator-nucleotide inhibitor ternary complex, nuclease H124L-thymidine 3',5'-bisphosphate-Ca2+, was inves...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00453a011

    authors: Wang JF,LeMaster DM,Markley JL

    更新日期:1990-01-09 00:00:00

  • Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments.

    abstract::Recombinant 15N-labeled human interleukin 2 (IL-2) has been studied by 2D and 3D NMR using uniformly 15N-labeled protein. Assignment of the backbone resonances has enabled the secondary structure of the protein to be defined. The secondary structure was found to consist of four alpha-helical regions and a short sectio...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00148a040

    authors: Mott HR,Driscoll PC,Boyd J,Cooke RM,Weir MP,Campbell ID

    更新日期:1992-08-25 00:00:00

  • Structural analysis of ADP-glucose pyrophosphorylase from the bacterium Agrobacterium tumefaciens.

    abstract::ADP-glucose pyrophosphorylase (ADPGlc PPase) catalyzes the conversion of glucose 1-phosphate and ATP to ADP-glucose and pyrophosphate. As a key step in glucan synthesis, the ADPGlc PPases are highly regulated by allosteric activators and inhibitors in accord with the carbon metabolism pathways of the organism. Crystal...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi701933q

    authors: Cupp-Vickery JR,Igarashi RY,Perez M,Poland M,Meyer CR

    更新日期:2008-04-15 00:00:00

  • Quantitative studies on the melittin-induced leakage mechanism of lipid vesicles.

    abstract::We have investigated, both experimentally and theoretically, the efflux of carboxyfluorescein (a self-quenching fluorescent dye) from vesicles of different sizes and lipid species (POPC, DOPC) after having added the bee venom peptide melittin. This comprises quantitative analyses regarding the extent of lipid-associat...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi971009p

    authors: Rex S,Schwarz G

    更新日期:1998-02-24 00:00:00

  • Unfolding rates of barstar determined in native and low denaturant conditions indicate the presence of intermediates.

    abstract::The folding and unfolding rates of the small protein, barstar, have been monitored using stopped-flow measurements of intrinsic tryptophan fluorescence at 25 degrees C, pH 8.5, and have been compared over a wide range of urea and guanidine hydrochloride (GdnHCl) concentrations. When the logarithms of the rates of fold...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi011494v

    authors: Sridevi K,Udgaonkar JB

    更新日期:2002-02-05 00:00:00

  • Structural basis for the quinone reduction in the bc1 complex: a comparative analysis of crystal structures of mitochondrial cytochrome bc1 with bound substrate and inhibitors at the Qi site.

    abstract::Cytochrome bc(1) is an integral membrane protein complex essential to cellular respiration and photosynthesis. The Q cycle reaction mechanism of bc(1) postulates a separated quinone reduction (Q(i)) and quinol oxidation (Q(o)) site. In a complete catalytic cycle, a quinone molecule at the Q(i) site receives two electr...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0341814

    authors: Gao X,Wen X,Esser L,Quinn B,Yu L,Yu CA,Xia D

    更新日期:2003-08-05 00:00:00

  • Investigation of the active site cysteine residue of rat liver mitochondrial aldehyde dehydrogenase by site-directed mutagenesis.

    abstract::To determine the active site cysteine residue in aldehyde dehydrogenase, we mutated amino acid residues 49, 162, and 302 of recombinantly expressed rat liver mitochondrial (class 2) aldehyde dehydrogenase. The C49A and C162A mutants were fully active tetrameric enzymes, although the C162A mutant was found to be highly...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00008a025

    authors: Farrés J,Wang TT,Cunningham SJ,Weiner H

    更新日期:1995-02-28 00:00:00

  • Flavin conformational changes in the catalytic cycle of p-hydroxybenzoate hydroxylase substituted with 6-azido- and 6-aminoflavin adenine dinucleotide.

    abstract::Crystallographic studies have demonstrated two flavin conformations for p-hydroxybenzoate hydroxylase (PHBH) [Gatti, D. L., Palfey, B. A. , Lah, M. S., Entsch, B., Massey, V., Ballou, D. P., & Ludwig, M. L. (1994) Science 266, 110-114. Schreuder, H. A., Mattevi, A., Obmolova, G., Kalk, K. H., Hol, W. G. J., van der Bo...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi971427u

    authors: Palfey BA,Ballou DP,Massey V

    更新日期:1997-12-16 00:00:00

  • Potent bifunctional anticoagulants: Kunitz domain-tissue factor fusion proteins.

    abstract::A strategy to design potent antagonists of human coagulation factor VIIa (FVIIa) by linking two proteins that independently inhibit activity and bind at separate, nonoverlapping sites is presented. A bifunctional inhibitor (KDTF5), comprising a Kunitz-type domain engineered to inhibit the FVIIa active site and a solub...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi970388j

    authors: Lee GF,Lazarus RA,Kelley RF

    更新日期:1997-05-13 00:00:00

  • Hydrogen bond interactions of the pheophytin electron acceptor and its radical anion in photosystem II as revealed by Fourier transform infrared difference spectroscopy.

    abstract::The primary electron acceptor pheophytin (Pheo(D1)) plays a crucial role in regulation of forward and backward electron transfer in photosystem II (PSII). It is known that some cyanobacteria control the Pheo(D1) potential in high-light acclimation by exchanging the D1 proteins from different copies of the psbA genes. ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9018829

    authors: Shibuya Y,Takahashi R,Okubo T,Suzuki H,Sugiura M,Noguchi T

    更新日期:2010-01-26 00:00:00

  • Interaction of 4-azido-2-nitrophenyl phosphate, a pseudosubstrate, with the sarcoplasmic reticulum Ca-ATPase.

    abstract::In the dark, 4-azido-2-nitrophenyl phosphate (ANPP) is a phosphate analog which behaves like a simple energy-rich phosphate donor for the sarcoplasmic reticulum (SR) Ca-ATPase. Like p-nitrophenyl phosphate (pNPP), ANPP is hydrolyzed by the enzyme only in the presence of calcium and magnesium (K0.5 and Vmax are 0.3 mM ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00175a030

    authors: Lacapère JJ,Garin J

    更新日期:1994-03-08 00:00:00

  • Probing inhibitor-induced conformational changes along the interface between tissue factor and factor VIIa.

    abstract::Upon injury of a blood vessel, activated factor VII (FVIIa) forms a high-affinity complex with its allosteric regulator, tissue factor (TF), and initiates blood clotting. Active site-inhibited factor VIIa (FVIIai) binds to TF with even higher affinity. We compared the interactions of FVIIai and FVIIa with soluble TF (...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi010283n

    authors: Osterlund M,Owenius R,Carlsson K,Carlsson U,Persson E,Lindgren M,Freskgård PO,Svensson M

    更新日期:2001-08-07 00:00:00