Evidence for an unprecedented histidine hydroxyl modification on D2-His336 in Photosystem II of Thermosynechoccocus vulcanus and Thermosynechoccocus elongatus.

abstract：:Previous studies of Escherichia coli dihydrofolate reductase (ecDHFR) have demonstrated that residue G121, which is 19 A from the catalytic center, is involved in catalysis, and long distance dynamical motions were implied. Specifically, the ecDHFR mutant G121V has been extensively studied by various experimental and ...

journal_title：Biochemistry

authors： Wang L,Tharp S,Selzer T,Benkovic SJ,Kohen A

更新日期：2006-02-07 00:00:00

abstract：:Cibacron Blue F3GA, the polycyclic blue chromophore of Blue Dextran 2000, inhibits the catalytic subunit of bovine brain protein kinase. The rate of inactivation exhibits a hyperbolic dependence on the dye concentration. This suggests that an enzyme-dye complex forms prior to inactivation. Protein and peptide substrat...

journal_title：Biochemistry

authors： Witt JJ,Roskoski R Jr

更新日期：1980-01-08 00:00:00

abstract：:New homo- and heterobifunctional cross-linking reagents have been synthesized. These reagents are based on ortho ester, acetal, and ketal functionalities that undergo acid-catalyzed dissociation but are base stable. The protein-reactive group in all the homobifunctional reagents is a maleimide group; the heterobifunct...

journal_title：Biochemistry

authors： Srinivasachar K,Neville DM Jr

更新日期：1989-03-21 00:00:00

abstract：:Locked nucleic acid (LNA) is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. LNA oligonucleotides display unprecedented hybridization affinity toward complementary single-stranded RNA and complementary single- or double...

journal_title：Biochemistry

authors： Vester B,Wengel J

更新日期：2004-10-26 00:00:00

abstract：:Translocator protein 18 kDa (TSPO) was previously known as the peripheral benzodiazepine receptor (PBR) in eukaryotes, where it is mainly localized to the mitochondrial outer membrane. Considerable evidence indicates that it plays regulatory roles in steroidogenesis and apoptosis and is involved in various human disea...

journal_title：Biochemistry

authors： Li F,Liu J,Liu N,Kuhn LA,Garavito RM,Ferguson-Miller S

更新日期：2016-05-24 00:00:00

abstract：:The [2Fe-2S] cluster of the Rieske iron-sulfur protein is held between two loops of the protein that are connected by a disulfide bridge. We have replaced the two cysteines that form the disulfide bridge in the Rieske protein of Saccharomyces cerevisiae with tyrosine and leucine, and tyrosine and valine, to evaluate t...

journal_title：Biochemistry

authors： Merbitz-Zahradnik T,Zwicker K,Nett JH,Link TA,Trumpower BL

更新日期：2003-11-25 00:00:00

abstract：:Methyl esterification of carboxylic acid residues in intact mouse S49 lymphoma cells was examined, and at least 24 proteins were found to be modified. Cell fractionation revealed that a distinct set of these proteins could be found in each of the four fractions. Nuclei contained 11 methyl-esterified proteins at 12, 15...

journal_title：Biochemistry

authors： Chelsky D,Ruskin B,Koshland DE Jr

更新日期：1985-11-05 00:00:00

abstract：:Halogen bonding is a weak chemical force that has so far mostly found applications in crystal engineering. Despite its potential for use in drug discovery, as a new molecular tool in the direction of molecular recognition events, it has rarely been assessed in biopolymers. Motivated by this fact, we have developed a p...

journal_title：Biochemistry

authors： Danelius E,Andersson H,Jarvoll P,Lood K,Gräfenstein J,Erdélyi M

更新日期：2017-06-27 00:00:00

abstract：:To identify how many rhodopsin intermediates interact with retinal G-protein transducin, the photobleaching process of chicken rhodopsin has been investigated in the presence or absence of transducin by means of time-resolved low-temperature spectroscopy. Singular value decomposition (SVD) analysis of the spectral dat...

journal_title：Biochemistry

authors： Tachibanaki S,Imai H,Mizukami T,Okada T,Imamoto Y,Matsuda T,Fukada Y,Terakita A,Shichida Y

更新日期：1997-11-18 00:00:00

abstract：:The intermediates in the oxidation of deoxyhemoglobin by ferricyanide in 0.1 M KCl, at 20 degrees C and three pH values, were studied by cryogenic techniques. Data analysis was carried out according to a simple four rate constant model, ignoring the functional heterogeneity of the subunits, to simulate the time course...

journal_title：Biochemistry

authors： Perrella M,Shrager RI,Ripamonti M,Manfredi G,Berger RL,Rossi-Bernardi L

更新日期：1993-05-18 00:00:00

abstract：:Despite much work, subcellular neurons of Caenorhabditis elegans have not been studied at nanometer resolution with millisecond time resolution. Nor has there been an effective way to immobilize C. elegans. Here we show that, without using anesthetic or paralyzing agents, fluorescence imaging with one-nanometer accura...

journal_title：Biochemistry

authors： Kural C,Nonet ML,Selvin PR

更新日期：2009-06-09 00:00:00

abstract：:Prosthetic groups such as heme, chlorophyll, and cobalamin (vitamin B(12)) are characterized by their branched biosynthetic pathway and unique metal insertion steps. The metal ion chelatases can be broadly classed either as single-subunit ATP-independent enzymes, such as the anaerobic cobalt chelatase and the protopor...

journal_title：Biochemistry

authors： Schubert HL,Raux E,Wilson KS,Warren MJ

更新日期：1999-08-17 00:00:00

abstract：:The second-order electron transfer reaction between cytochrome b5 and cytochrome c has been studied by cyclic voltammetry utilizing a gold electrode modified with beta-mercaptopropionate. When cyclic voltammetry is performed on a solution containing a mixture of cytochrome b5, cytochrome c and polylysine, cytochrome b...

journal_title：Biochemistry

authors： Seetharaman R,White SP,Rivera M

更新日期：1996-09-24 00:00:00

abstract：:We examine the relationship between binding affinity and interface size for reversible protein-protein interactions (PPIs), using cytokines from the tumor necrosis factor (TNF) superfamily and their receptors as a test case. Using surface plasmon resonance, we measured single-site binding affinities for binding of the...

journal_title：Biochemistry

authors： Day ES,Cote SM,Whitty A

更新日期：2012-11-13 00:00:00

abstract：:Because of the conflicting conclusions that have been reached regarding the location of the two putative membrane-spanning segments from cysteine 911 through isoleucine 929 and from isoleucine 946 through cysteine 964 in the alpha subunit of native ovine Na+/K(+)-transporting ATPase, the disposition of lysine 943 with...

journal_title：Biochemistry

authors： Anderberg SJ

更新日期：1995-07-25 00:00:00

abstract：:The kinetics of Zn(2+) binding by two point-mutated forms of the HIV-1 NCp7 C-terminal zinc finger, each containing tridentate binding motif HCC [Ser49(35-50)NCp7] or CCC [Ala44(35-50)NCp7], has been studied by stopped-flow spectrofluorimetry. Both the formation and dissociation rate constants of the complexes between...

journal_title：Biochemistry

authors： Bombarda E,Roques BP,Mély Y,Grell E

更新日期：2005-05-17 00:00:00

abstract：:The effect of concentrated glycerol on the thermal transitions of chymotrypsinogen and ribonuclease has been examined by differential spectrophotometry at 293 and 287 mm, respectively. It was found that for both proteins addition of glycerol raises the transition temperature, the increase in Tm being greater for ribon...

journal_title：Biochemistry

authors： Gekko K,Timasheff SN

更新日期：1981-08-04 00:00:00

abstract：:Staphylococcus aureus transcription factor QacR regulates expression of the qacA multidrug efflux determinant. In response to binding cationic lipophilic compounds, including ethidium and rhodamine 6G, QacR dissociates from the qacA operator alleviating repression. Such ligand binding uniformly induces a coil-to-helix...

journal_title：Biochemistry

authors： Peters KM,Sharbeen G,Theis T,Skurray RA,Brown MH

更新日期：2009-10-20 00:00:00

abstract：:The enzyme steroid 5 alpha-reductase utilizes NADPH to reduce the double bonds of a variety of steroid substrates with generalized 3-oxo, delta 4,5 structures. One substrate for this membrane-bound enzyme is testosterone, whose reduction to dihydrotestosterone is required for the embryonic differentiation of the exter...

journal_title：Biochemistry

authors： Wigley WC,Prihoda JS,Mowszowicz I,Mendonca BB,New MI,Wilson JD,Russell DW

更新日期：1994-02-08 00:00:00

abstract：:Inexpensive, straightforward, and rapid medical diagnostics are becoming increasingly important for disease identification in time- and resource-limited settings. Previous attempts to link oligonucleotide-based aptamers and hammerhead ribozymes to form ligand-induced ribozymes have been successful in identifying a var...

journal_title：Biochemistry

authors： Liao AM,Pan W,Benson JC,Wong AD,Rose BJ,Caltagirone GT

更新日期：2018-08-28 00:00:00

abstract：:The structure of [14-38]Abu, a variant of bovine pancreatic trypsin inhibitor (BPTI) with only the 14-38 disulfide bridge intact, has been analyzed by two-dimensional 1H and 1H-15N NMR. Except for the 18-24, 29-35 antiparallel beta-sheet, residues in all regions of the molecule give two exchange cross peaks for each 1...

journal_title：Biochemistry

authors： Barbar E,Barany G,Woodward C

更新日期：1995-09-12 00:00:00

abstract：:We assigned l-talarate dehydratase (TalrD) and galactarate dehydratase (GalrD) functions to a group of orthologous proteins in the mechanistically diverse enolase superfamily, focusing our characterization on the protein encoded by the Salmonella typhimurium LT2 genome (GI:16766982; STM3697). Like the homologous mande...

journal_title：Biochemistry

authors： Yew WS,Fedorov AA,Fedorov EV,Almo SC,Gerlt JA

更新日期：2007-08-21 00:00:00

abstract：:The orientation of eukaryotic cytochromes P450, with respect to the membrane of the endoplasmic reticulum, has been investigated. There is now good evidence that the tertiary structure of these proteins is essentially the same as that of the soluble bacterial isoenzyme cytochrome P450CI, with the exception of an exten...

journal_title：Biochemistry

authors： Edwards RJ,Murray BP,Singleton AM,Boobis AR

更新日期：1991-01-08 00:00:00

abstract：:31P NMR measurements were made (at 121.5 MHz and 5 degrees C) on enzyme-bound substrate complexes of 3-phosphoglycerate kinase in order to address three questions pertaining to (i) the integrity of the enzyme-substrate complexes with Mg(II) in the presence of sulfate concentrations typical of those used for crystalliz...

journal_title：Biochemistry

authors： Ray BD,Rao BD

更新日期：1988-07-26 00:00:00

abstract：:Vitamin D receptor (VDR) is a member of the nuclear hormone receptor superfamily. When bound to a variety of vitamin D analogues, VDR manifests a wide diversity of physiological actions. The molecular mechanism by which different vitamin D analogues cause specific responses is not understood. The published crystallogr...

journal_title：Biochemistry

authors： Singarapu KK,Zhu J,Tonelli M,Rao H,Assadi-Porter FM,Westler WM,DeLuca HF,Markley JL

更新日期：2011-12-27 00:00:00

abstract：:The self-splicing ai5g group II intron was transformed into a three-part ribozyme that site-specifically cleaves small oligonucleotide substrates with multiple turnover. The ribozyme is composed of intron domain 1 (D1, 425 nucleotides), with catalytically essential domain 5 (D5, 58 nucleotides) provided separately as ...

journal_title：Biochemistry

authors： Michels WJ Jr,Pyle AM

更新日期：1995-03-07 00:00:00

abstract：:Thermal transitions of type I collagen fibrils were investigated by differential scanning calorimetry and spectrophotometry of turbidity within a wide range of external conditions. The advanced microcalorimeter allowed us to carry out the measurements at low concentrations of collagen (0.15-0.3 mg/mL). At these concen...

journal_title：Biochemistry

authors： Tiktopulo EI,Kajava AV

更新日期：1998-06-02 00:00:00

abstract：:The association of membrane and cytoplasmic proteins with the cytoskeleton of resting and activated platelets was studied. Glycoproteins were identified by labeling with 125I-labeled lectins (concanavalin A, wheat germ agglutinin, and Lens culinaris). Polypeptides, which are embedded in the lipid bilayer, have been id...

journal_title：Biochemistry

authors： Rotman A,Heldman J,Linder S

更新日期：1982-04-13 00:00:00

abstract：:Nramp (natural resistance-associated macrophage protein) family members have been characterized in mammals, yeast, and bacteria as divalent metal ion/H(+) symporters. In previous work, a bioinformatic approach was used for the identification of residues that are conserved within the Nramp family [Haemig, H. A., and Br...

journal_title：Biochemistry

authors： Haemig HA,Moen PJ,Brooker RJ

更新日期：2010-06-08 00:00:00

abstract：:The aminoacylase-1/metallopeptidase 20 (Acy1/M20) family features several l-aminoacylases useful in biocatalysis. Mammalian Acy1, in particular, has been applied in racemic resolution and reverse hydrolysis. Despite recent advances in our understanding of the active site architecture and functioning, determinants of A...

journal_title：Biochemistry

authors： Lindner HA,Alary A,Wilke M,Sulea T

更新日期：2008-04-08 00:00:00