Abstract：
:We have studied a group of fusion peptides of influenza hemagglutinin in which the N-terminal amino acid, Gly (found in the wild-type peptide), has been systematically substituted with Ala, Ser, Val, or Glu. The activity of the intact hemagglutinin protein with these same substitutions has already been reported. As a measure of the extent of modulation of intrinsic membrane curvature by these peptides, we determined their effects on the polymorphic phase transition of dipalmitoleoylphosphatidylethanolamine. The wild-type peptide is the only one that, at pH 5, can substantially decrease the temperature of this transition. This is also the only form in which the intact protein promotes contents mixing in cells. The Ala and Ser mutant hemagglutinins exhibit a hemifusion phenotype, and their fusion peptides have little effect on lipid polymorphism at low pH. The two mutant proteins that are completely fusion inactive are the Val and Glu mutant hemagglutinins. The fusion peptides from these forms significantly increase the polymorphic phase transition temperature at low pH. We find that the effect of the fusion peptides on membrane curvature, as monitored by a shift in the temperature of this polymorphic phase transition, correlates better with the fusogenic activities of the corresponding protein than do measurements of the isotropic (31)P NMR signals or the ability to induce the fusion of liposomes. The inactivity of the hemagglutinin protein with the hydrophobic Val mutation can be explained by the change in the angle of membrane insertion of the helical fusion peptide as measured by polarized FTIR. Thus, the nature of the interactions of the fusion peptides with membranes can, in large part, explain the differences in the fusogenic activity of the intact protein.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Epand RM,Epand RF,Martin I,Ruysschaert JMdoi
10.1021/bi0107187subject
Has Abstractpub_date
2001-07-31 00:00:00pages
8800-7issue
30eissn
0006-2960issn
1520-4995pii
bi0107187journal_volume
40pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The formation of amyloid fibril is associated with major human diseases, including Alzheimer's disease, prion diseases, and type 2 diabetes. Methods for efficient inhibition of amyloid fibril formation are therefore highly clinically important. A principal approach for the inhibition of amyloid formation is based on t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048582a
更新日期：2004-11-16 00:00:00
abstract：:Chloride intracellular channel protein 1 (CLIC1) functions as an anion channel in plasma and nuclear membranes when its soluble monomeric form converts to an integral-membrane form. The transmembrane region of CLIC1 is located in its thioredoxin-like domain 1, but the mechanism whereby the protein converts to its memb...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9010607
更新日期：2009-09-08 00:00:00
abstract：:Dodecameric glutamine synthetase (GS) from bacteria is formed from two face-to-face hexameric rings of identical subunits. These highly symmetrical aggregates from some bacteria, including Escherichia coli, "stack" in the presence of Zn2+ and other divalent ions to generate protein tubes (phase I) and subsequently ass...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00254a001
更新日期：1994-12-20 00:00:00
abstract：:Carboxymethylation with 14 C-labeled iodoacetate of cysteine residues in wild-type beta-galactosidase from Escherichia coli and in a defective beta-galactosidase from deletion mutant strain M15 was investigated in order to determine accessible positions in the tetrameric wild-type form and the dimeric mutant M15 prote...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00617a015
更新日期：1978-11-28 00:00:00
abstract：:Calcium protects a limited number of embryonic chick neural retina cell surface proteins from tryptic cleavage. One glycoprotein of Mr approximately 1.3 X 10(5) and pI approximately 4.8 (gp130) is present in intact retinas and, in the presence of Ca2+, is both resistant to tryptic cleavage and poorly iodinated. When c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00300a016
更新日期：1984-02-28 00:00:00
abstract：:Escherichia coli DNA photolyase contains a stable flavin radical that is readily photoreduced in the presence of added electron donors. Picosecond, nanosecond, and conventional flash photolysis technique have been employed to investigate the events leading to photoreduction from 40 ps to tens of milliseconds following...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00476a008
更新日期：1990-06-19 00:00:00
abstract：:A conserved, crystallographically defined bile acid binding site was originally identified in the membrane domain of mammalian and bacterial cytochrome c oxidase (CcO). Current studies show other amphipathic molecules including detergents, fatty acids, steroids, and porphyrins bind to this site and affect the already ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi3014505
更新日期：2013-02-26 00:00:00
abstract：:The composition of the molybdate-stabilized glucocorticoid receptor (GR) complex has been investigated with a monoclonal antibody against the steroid-binding Mr 94 000 (94K) GR protein. It was concluded that one antibody molecule binds one 94K GR molecule. This finding constituted the basis for calculating the number ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00344a041
更新日期：1985-11-05 00:00:00
abstract：:Photolyzed rhodopsin is phosphorylated at multiple serine and threonine residues during the quenching of phototransduction. Sites of phosphorylation by rhodopsin kinase have been localized to the C-terminal region of rhodopsin, but no information was available on the kinetics and identity of phosphorylated residues. T...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00072a030
更新日期：1993-06-01 00:00:00
abstract：:Effects of human insulin on glucose metabolism in the yeast Saccharomyces cerevisiae were studied in this report. Under two conditions of growth limitation (glucose-grown cells during transition to stationary phase or spheroplasts during incubation in synthetic glucose medium), human insulin (10 and 1 microM, respecti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972071p
更新日期：1998-06-16 00:00:00
abstract：:An equimolar mixture of DL-[alpha-2H]- and DL-[alpha-13C]mandelate, when incubated with mandelate racemase (EC 5.1.2.2), shows conversion of singly labeled mandelate to unlabeled mandelate, due to solvent exchange of the alpha proton, while the level of doubly labeled mandelate remains at a constant low level. Similar...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00625a015
更新日期：1977-03-22 00:00:00
abstract：:The Pf1 gene 5 protein forms a large helical nucleoprotein complex (Mr = 3.1 x 10(7)) with single-stranded viral DNA, from which a 32 amino acid sequence rich in alanine, proline, and glutamine residues can be removed from the C-terminus by limited proteolysis. Sharp resonances in the 1H NMR spectrum of the Pf1 nucleo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00001a018
更新日期：1995-01-10 00:00:00
abstract：:The conformational properties of an active-site loop segment, defined by residues Ser(161)-Glu(162)-Asn(163)-Ser(164), have been shown to be important for modulating the intrinsic reactivity of Mn(II) in the active site of Bacillus subtilis oxalate decarboxylase. We now detail the functional and structural consequence...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300001q
更新日期：2012-04-03 00:00:00
abstract：:Cardiac troponin T (cTnT) is a myofibrillar protein essential for calcium-dependent contraction. This property has led to functional studies of developmentally expressed cTnT isoforms and mutants identified in patients with hypertrophic cardiomyopathy. The release of cTnT into the serum following myocardial infarction...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7012596
更新日期：2008-02-19 00:00:00
abstract：:The folding of the peptide chain of the bovine heart oxoglutarate carrier in the inner mitochondrial membrane and in the membrane of reconstituted proteoliposomes has been investigated by enzymatic and immunochemical approaches using proteinase K and polyclonal site-directed antibodies, respectively. Two peptides corr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00178a030
更新日期：1994-03-29 00:00:00
abstract：:Eukaryotic transfer RNA precursors (pre-tRNAs) contain a 5' leader preceding the aminoacyl acceptor stem and a 3' trailer extending beyond this stem. An early step in pre-tRNA maturation is removal of the 5' leader by the endoribonuclease, RNase P. Extensive pairing between leader and trailer sequences has previously ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000603n
更新日期：2000-08-15 00:00:00
abstract：:The role of the Na,K-ATPase beta-subunit in stabilization of ion-binding sites has been investigated. Treatment of the purified renal Na,K-ATPase with 0.25 M DTT at 40 degrees C for 1 h resulted in 50% loss of Rb occlusion, which correlates with partial reduction of S-S bridges in the extracellular portion of the beta...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00077a029
更新日期：1993-07-06 00:00:00
abstract：:A major biochemical goal is the ability to mimic nature in engineering highly specific protein-protein interactions (PPIs). We previously devised a computational interactome screen to identify eight peptides that form four heterospecific dimers despite 32 potential off-targets. To expand the speed and utility of our a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00047
更新日期：2017-03-21 00:00:00
abstract：:A steady-state kinetic analysis was conducted of the overall aminoacylation reaction catalyzed by isoleucyl-tRNA synthetase. The patterns of Lineweaver-Burk plots obtained indicated that tRNA adds to the enzyme only after isoleucyl adenylate formation and pyrophosphate release. These kinetic patterns were consistent w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00580a018
更新日期：1979-06-26 00:00:00
abstract：:Kinetic studies of the effect of addition of Co2+ or Mn2+ to a highly purified dipeptidase from Ehrlich-Lettré mouse ascites tumor cells show that these metals specifically activate the hydrolyses of certain classes of dipeptides. This enzyme was previously (S. Hayman and E. K. Patterson, 1971, J. Biol. Chem. 246, 660...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00690a018
更新日期：1975-09-23 00:00:00
abstract：:The preferential interactions of proteins with solvent components were studied in concentrated aqueous solutions of the sulfate, acetate, and chloride salts of Mg2+, Ba2+, Ca2+, Mn2+ and Ni2+ [except for CaSO4, BaSO4, Mn-(OAc)2, and Ni(OAc)2], and results were compared with those of the Na+ salts. It was found that, f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00320a004
更新日期：1984-12-04 00:00:00
abstract：:In type 2 diabetics, the hormone amylin misfolds into amyloid plaques implicated in the destruction of the pancreatic β-cells that make insulin and amylin. The aggregative misfolding of amylin is pH-dependent, and exposure of the hormone to acidic and basic environments could be physiologically important. Amylin has t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401164k
更新日期：2014-01-21 00:00:00
abstract：:Redox transitions in the Rhodobacter capsulatus cytochrome bc(1) complex were investigated by perfusion-induced attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy combined with synchronous visible spectroscopy, in both the wild type and a cytochrome c(1) point mutant, M183K, in which the mi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049211x
更新日期：2004-07-27 00:00:00
abstract：:The respiratory chain complexes can arrange into multienzyme assemblies, so-called supercomplexes. We present the first 3D map of a respiratory chain supercomplex. It was determined by random conical tilt electron microscopy analysis of a bovine supercomplex consisting of complex I, dimeric complex III, and complex IV...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700983h
更新日期：2007-11-06 00:00:00
abstract：:The multimeric cytochromes described to date in sulfate- and sulfur-reducing bacteria are associated with diverse respiratory modes involving the use of elemental sulfur or oxidized sulfur compounds as terminal acceptors. They exhibit no structural similarity with the other cytochrome c classes and are characterized b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9707741
更新日期：1997-09-02 00:00:00
abstract：:CRISPR-Cas9 technology has been at the forefront of the field of biology. The Streptococcus pyogenes (SpyCas9) protein forms a complex with guide RNA and can recognize and cleave double-stranded DNA through hybridization based on 20 base pairings. SpyCas9 has two nuclease domains, HNH and RuvC, each of which cuts each...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00766
更新日期：2021-01-26 00:00:00
abstract：:Ultraviolet absorption techniques were used to study the thermodynamics of duplex formation for a DNA decamer, d(GCGAAAAGCG).d(CGCTTTTCGC), and a series of related duplexes, each of which contains a bulged base centered in the A.T tract. Thermodynamic parameters were obtained from nonlinear least-squares fits of the m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00230a031
更新日期：1991-04-23 00:00:00
abstract：:The effect of the polyene antibiotic amphotericin B on the electron paramagnetic resonance spectra of lipid probes intercalated in model membranes was examined. When the antibiotic was added to the aqueous phase, no spectral effects occurred. However, when the antibiotic was incorporated during membrane preparation, c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00523a010
更新日期：1981-09-29 00:00:00
abstract：:Apical membrane antigen 1 (AMA1) of the human malaria parasite Plasmodium falciparum is synthesized by schizont stage parasites and has been implicated in merozoite invasion of host erythrocytes. Phage-display techniques have recently been used to identify two 15-residue peptides, F1 and F2, which bind specifically to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034376b
更新日期：2003-08-26 00:00:00
abstract：:Gastric parietal cell apical membranes must protect the cell from the extremely low pH and wide variations in osmolality of the gastric juice. To characterize the permeability properties of gastric apical membranes, we have measured passive permeabilities to water, protons, NH3, and small nonelectrolytes of membrane v...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00061a002
更新日期：1993-03-16 00:00:00
