Probe of beta-galactosidase structure with iodoacetate. Differential reactivity of thiol groups in wild-type and mutant forms of beta-galactosidase.

abstract：:As part of a larger effort to engineer the stability and hemin-binding properties of microsomal (Mc) cytochromes b(5) into rat liver outer mitochondrial membrane (OM) cytochrome (cyt) b(5), several mutants of rat OM cyt b(5) were prepared to study the effect of gradual and complete elimination of two extended hydropho...

journal_title：Biochemistry

authors： Cowley AB,Altuve A,Kuchment O,Terzyan S,Zhang X,Rivera M,Benson DR

更新日期：2002-10-01 00:00:00

abstract：:DNA photolyase from Escherichia coli contains FAD plus a partially characterized, second chromophore. In vivo, the flavin is fully reduced (FADH2), but oxidation to a stable, blue radical (FADH.) occurs during enzyme isolation. The second chromophore is irreversibly reduced by reaction of the enzyme with sodium borohy...

journal_title：Biochemistry

authors： Jorns MS,Wang B,Jordan SP

更新日期：1987-10-20 00:00:00

abstract：:Type II dihydrofolate reductases (DHFRs) encoded by the R67 and R388 plasmids are different both in sequence and in structure from known chromosomal DHFRs. These plasmid-derived DHFRs are responsible for conferring trimethoprim resistance to the host strain. A derivative of R388 DHFR, RBG200, has been cloned and overp...

journal_title：Biochemistry

authors： Brito RM,Reddick R,Bennett GN,Rudolph FB,Rosevear PR

更新日期：1990-10-23 00:00:00

abstract：:Retinoids function through conformational alterations of ligand-dependent nuclear transcription factors, the retinoic acid receptors, and retinoid X receptors. 9-cis-Retinoic acid is a known biological ligand for retinoid X receptors, but its synthesis pathway in vivo is largely unknown. Recently, we identified a cis-...

journal_title：Biochemistry

authors： Paik J,Vogel S,Piantedosi R,Sykes A,Blaner WS,Swisshelm K

更新日期：2000-07-11 00:00:00

abstract：:Pairing rules have been developed to predict the sequence specificity of minor groove binding polyamides containing pyrrole (Py) and imidazole (Im) amino acids. An Im/Py pair distinguishes G.C from C.G and both of these from A.T/T.A base pairs. A Py/Py pair appears not to distinguish A.T from T.A base pairs. To test t...

journal_title：Biochemistry

authors： White S,Baird EE,Dervan PB

更新日期：1996-09-24 00:00:00

abstract：:The mechanism of inactivation of human leukocyte elastase (HLE) by the chloromethyl ketone MeOSuc-Ala-Ala-Pro-Val-CH2Cl was investigated. The dependence of the first-order rate constant for inactivation on concentration of chloromethyl ketone is hyperbolic and suggests formation of a reversible "Michaelis complex" pri...

journal_title：Biochemistry

authors： Stein RL,Trainor DA

更新日期：1986-09-23 00:00:00

abstract：:Design of a partially cysteine-depleted C98S/C239S/C377S/C468A cytochrome P450 3A4 mutant designated CYP3A4(C58,C64) allowed site-directed incorporation of thiol-reactive fluorescent probes into alpha-helix A. The site of modification was identified as Cys-64 with the help of CYP3A4(C58) and CYP3A4(C64), each bearing ...

journal_title：Biochemistry

authors： Tsalkova TN,Davydova NY,Halpert JR,Davydov DR

更新日期：2007-01-09 00:00:00

abstract：:Derivatives of imidazole-2-thiones block reactions catalyzed by thyroid peroxidase (TPX) and the closely related lactoperoxidase (LPX), and this property is used therapeutically to treat hyperthyroidism. The reactions of a series of benzimidazoline-2-thiones with chemical and enzymatic oxidants were investigated to pr...

journal_title：Biochemistry

authors： Doerge DR,Decker CJ,Takazawa RS

更新日期：1993-01-12 00:00:00

abstract：:Kinetic and structural data are presented on the interaction with Torpedo californica acetylcholinesterase (TcAChE) of (+)-huperzine A, a synthetic enantiomer of the anti-Alzheimer drug, (-)-huperzine A, and of its natural homologue (-)-huperzine B. (+)-Huperzine A and (-)-huperzine B bind to the enzyme with dissociat...

journal_title：Biochemistry

authors： Dvir H,Jiang HL,Wong DM,Harel M,Chetrit M,He XC,Jin GY,Yu GL,Tang XC,Silman I,Bai DL,Sussman JL

更新日期：2002-09-03 00:00:00

abstract：:The cytoplasmic disassembly of Ran.GTP.importin and Ran.GTP.exportin. cargo complexes is an essential step in the corresponding nuclear import and export cycles. It has previously been shown that such disassembly can be mediated by RanBP1 in the presence of RanGAP. The nuclear pore complex protein RanBP2 (Nup358) cont...

journal_title：Biochemistry

authors： Villa Braslavsky CI,Nowak C,Görlich D,Wittinghofer A,Kuhlmann J

更新日期：2000-09-26 00:00:00

abstract：:We have studied the Cu(II), Co(II), and Fe(III) complexes of the antineoplastic drug bleomycin by using electron spin--echo envelope spectroscopy. For all three complexes, nitrogen coordination of the metal ions is demonstrated. For the Cu(II)-- and Co(II)--drug complexes, we have been able to identify imidazole as a ...

journal_title：Biochemistry

authors： Burger RM,Adler AD,Horwitz SB,Mims WB,Peisach J

更新日期：1981-03-17 00:00:00

abstract：:To identify regions of the Torpedo Na,K-ATPase alpha-subunit that interact with membrane lipid and to characterize conformationally dependent structural changes in the transmembrane domain, we have proteolytically mapped the sites of photoincorporation of the hydrophobic compounds 3-(trifluoromethyl)-3-(m-[(125)I]iodo...

journal_title：Biochemistry

authors： Blanton MP,McCardy EA

更新日期：2000-11-07 00:00:00

abstract：:An investigation into the kinetics and regulatory properties of the type-1 phosphofructokinase (PFK) from the extreme thermophile Thermus thermophilus (TtPFK) reveals an enzyme that is inhibited by PEP and activated by ADP by modifying the affinity exhibited for the substrate fructose 6-phosphate (Fru-6-P) in a manner...

journal_title：Biochemistry

authors： McGresham MS,Lovingshimer M,Reinhart GD

更新日期：2014-01-14 00:00:00

abstract：:Neuropeptide Y (NPY) and the pancreatic polypeptide (PP) are members of the neuropeptide Y family of hormones. They bind to the Y receptors with very different affinities: Whereas PP is highly selective for the Y(4) receptor, NPY displays highest affinites for Y(1), Y(2), and Y(5) receptor subtypes. Introducing the NP...

journal_title：Biochemistry

authors： Lerch M,Kamimori H,Folkers G,Aguilar MI,Beck-Sickinger AG,Zerbe O

更新日期：2005-06-28 00:00:00

abstract：:Vitamin K dependent carboxylation of an exogenous peptide substrate and endogenous protein substrates, vitamin K epoxidation, and reduction of vitamin K epoxide were measured in subcellular fractions from rat liver. The rough microsomal fraction was highly enriched in all four activities; lower levels were found in sm...

journal_title：Biochemistry

authors： Carlisle TL,Suttie JW

更新日期：1980-03-18 00:00:00

abstract：:The translocation (T) domain of diphtheria toxin plays a critical role in moving the catalytic domain across the endosomal membrane. Translocation/insertion is triggered by a decrease in pH in the endosome where conformational changes of T domain occur through several kinetic intermediates to yield a final trans-membr...

journal_title：Biochemistry

authors： Li J,Rodnin MV,Ladokhin AS,Gross ML

更新日期：2014-11-04 00:00:00

abstract：:Human ferrochelatase (EC 4.99.1.1) catalyzes the insertion ferrous iron into protoporphyrin IX as the last step in heme biosynthesis, an essential process to most organisms given the vast intracellular functions of heme. Even with multiple ferrochelatase structures available, the exact mechanism for iron insertion int...

journal_title：Biochemistry

authors： Asuru AP,An M,Busenlehner LS

更新日期：2012-09-11 00:00:00

abstract：:In the reaction center (RC) of the photosynthetic bacterium Rhodobacter sphaeroides, two ubiquinone molecules, QA and QB, play a pivotal role in the conversion of light energy into chemical free energy by coupling electron transfer to proton uptake. In native RCs, the transfer of an electron from QA to QB takes place ...

journal_title：Biochemistry

authors： Breton J

更新日期：2007-04-17 00:00:00

abstract：:Although insulin and insulin-like growth factor-1 (IGF-1) belong to one family, insulin folds into one thermodynamically stable structure, while IGF-1-folds into two thermodynamically stable structures (native and swap forms). We have demonstrated previously that the bifurcating folding behavior of IGF-1 is mainly con...

journal_title：Biochemistry

authors： Chen Y,You Y,Jin R,Guo ZY,Feng YM

更新日期：2004-07-20 00:00:00

abstract：:The pentraxin serum amyloid P component (SAP) is secreted by the liver and found in plasma at a concentration of approximately 30 mg/L. SAP is a 25 kDa homopentamer known to bind both protein and nonprotein ligands, all in a calcium-dependent manner. The function of SAP is unclear but likely involves the humoral innat...

journal_title：Biochemistry

authors： Poulsen ET,Pedersen KW,Marzeda AM,Enghild JJ

更新日期：2017-02-14 00:00:00

abstract：:There has been widespread interest in studying early intermediate states and their roles in protein folding. The interest in intermediate states has been further emphasized in the recent literature because of their implications for protein aggregation. Unfortunately, direct kinetic characterization of intermediates ha...

journal_title：Biochemistry

authors： Sarkar-Banerjee S,Chowdhury S,Paul SS,Dutta D,Ghosh A,Chattopadhyay K

更新日期：2016-08-16 00:00:00

abstract：:Piscidin 4, an antimicrobial peptide recently isolated from mast cells of hybrid striped bass (Morone chrysops female × Morone saxatilis male), is unusual in that it is twice as long (44 amino acids) as the typical members of the piscidin family. We previously showed that native piscidin 4 had a modified amino acid at...

journal_title：Biochemistry

authors： Park NG,Silphaduang U,Moon HS,Seo JK,Corrales J,Noga EJ

更新日期：2011-04-26 00:00:00

abstract：:Myosin belongs to the family of motor proteins. Its interaction with actin coupled with hydrolysis of ATP is the molecular basis of muscle contraction. The head segment of myosin, called subfragment 1 (S1), contains the distinct binding sites for ATP and actin and responsible for the ATPase activity. The rate-limiting...

journal_title：Biochemistry

authors： Peyser YM,Ben-Hur M,Werber MM,Muhlrad A

更新日期：1996-04-09 00:00:00

abstract：:NLRP4 is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family of cytosolic receptors and a member of an inflammation signaling cascade. Here, we present the crystal structure of the NLRP4 pyrin domain (PYD) at 2.3 Å resolution. The NLRP4 PYD is a member of the death domain (DD) superfamily ...

journal_title：Biochemistry

authors： Eibl C,Grigoriu S,Hessenberger M,Wenger J,Puehringer S,Pinheiro AS,Wagner RN,Proell M,Reed JC,Page R,Diederichs K,Peti W

更新日期：2012-09-18 00:00:00

abstract：:The backbone dynamics of Escherichia coli ribonuclease HI (RNase HI) in the picosecond to nanosecond time scale were characterized by a combination of measurements of 15N-NMR relaxation (T1, T2, and NOE), analyzed by a model-free approach, and molecular dynamics (MD) simulation in water. The MD simulations in water we...

journal_title：Biochemistry

authors： Yamasaki K,Saito M,Oobatake M,Kanaya S

更新日期：1995-05-23 00:00:00

abstract：:Proton NMR spectroscopy was applied to myoglobin in the ferric, water-liganded form (metMbH2O) and the apo form (apoMb) to probe the structure and stability of the latter. Proteins from sperm whale and horse skeletal muscles were studied to simplify the spectral assignment task. Nuclear Overhauser effects and the resp...

journal_title：Biochemistry

authors： Cocco MJ,Kao YH,Phillips AT,Lecomte JT

更新日期：1992-07-21 00:00:00

abstract：:Plasma membrane and vesicular transporters for the biogenic amines, dopamine, norepinephrine, and serotonin, represent a group of proteins that play a crucial role in the regulation of neurotransmission. Clinically, mono amine transporters are the primary targets for the actions of many therapeutic agents used to trea...

journal_title：Biochemistry

authors： Sager JJ,Torres GE

更新日期：2011-08-30 00:00:00

abstract：:Calcium dynamics and its linked molecular interactions cause a variety of biological responses; thus, exploiting techniques for detecting both concurrently is essential. Here we describe a method for measuring the cytosolic Ca(2+) concentration ([Ca(2+)](i)) and protein-protein interactions within the same cell, using...

journal_title：Biochemistry

authors： Mori MX,Imai Y,Itsuki K,Inoue R

更新日期：2011-05-31 00:00:00

abstract：:Polyphosphate glucokinase from Mycobacterium tuberculosis catalyzes the phosphorylation of glucose using inorganic polyphosphates [poly(P)] or ATP. The steady-state kinetic mechanisms of the poly(P)- and ATP-dependent glucokinase reactions were investigated using initial velocity, product inhibition, and dead-end inhi...

journal_title：Biochemistry

authors： Hsieh PC,Kowalczyk TH,Phillips NF

更新日期：1996-07-30 00:00:00

abstract：:The rate of 1-palmitoyl-2-[12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino] dodecanoyl] phosphatidylcholine (P-C12-NBD-PC) transfer between dioleoylphosphatidylcholine vesicles was measured by a technique based on resonance energy transfer between P-C12-NBD-PC and N-(lissamine rhodamine B sulfonyl)dioleoylphosphatidyletha...

journal_title：Biochemistry

authors： Nichols JW

更新日期：1986-08-12 00:00:00