Abstract：
:The rate of 1-palmitoyl-2-[12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino] dodecanoyl] phosphatidylcholine (P-C12-NBD-PC) transfer between dioleoylphosphatidylcholine vesicles was measured by a technique based on resonance energy transfer between P-C12-NBD-PC and N-(lissamine rhodamine B sulfonyl)dioleoylphosphatidylethanolamine [Nichols, J. W., & Pagano, R. E. (1982) Biochemistry 21, 1720-1726]. Addition of bile salts at concentrations below their critical micelle concentrations increased the rate of spontaneous P-C12-NBD-PC transfer without disrupting the vesicles. The effectiveness in increasing the transfer rate was dependent on the structure of the bile salt. In general, conjugated bile salts were more effective than unconjugated, and mono- and dihydroxy bile salts were more effective than trihydroxy. The kinetics of intervesicular P-C12-NBD-PC transfer in the presence of cholate were found to be consistent with a mass action kinetic model based on the premise that bile salts bind to the vesicles, alter the dissociation and/or association rate constants for phospholipid monomer-vesicle interaction, and increase the rate of phospholipid transfer via the diffusion of soluble monomers through the aqueous phase. Temperature dependence studies indicated that cholate binding to vesicles is an entropy-driven process and that cholate binding lowers the free energy of activation for phospholipid monomer-vesicle dissociation by producing compensatory decreases in both the enthalpy and entropy of activation.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Nichols JWdoi
10.1021/bi00364a021subject
Has Abstractpub_date
1986-08-12 00:00:00pages
4596-601issue
16eissn
0006-2960issn
1520-4995journal_volume
25pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Sso7d from the thermoacidophilic archaebacterium Sulfolobus solfataricus is a small globular protein with a known three-dimensional structure. Inspection of the structure reveals that Phe31 is a member of the aromatic cluster forming the protein hydrophobic core, whereas Trp23 is located on the protein surface and its...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972994k
更新日期：1998-07-21 00:00:00
abstract：:Introduction of specific structural probes into substrate binding sites of Escherichia coli glutamine synthetase is now possible. Various analogues of ATP substituted with an amino or sulfhydryl moiety at the 6- or 8-position of the purine ring have been found to substitute for ATP in the autoinactivation reaction of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00349a020
更新日期：1986-01-14 00:00:00
abstract：:Intrastrand cross-links represent the majority of modifications in DNA resulting from interaction with the cancer chemotherapeutic drug cis-diamminedichloroplatinum(II) (cis-DDP). These adducts were recently characterized although several discrepancies remained to be resolved. In these studies, [3H]-cis-dichloro(ethyl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00361a026
更新日期：1986-07-01 00:00:00
abstract：:We have combined three mutations previously shown to stabilize lambda repressor against thermal denaturation. Two of these mutations are in helix 3, where Gly-46 and Gly-48 have been replaced by alanines [Hecht, M. H., et al. (1986) Proteins: Struct., Funct., Genet. 1, 43-46]. The other mutation, which replaces Tyr-88...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00419a059
更新日期：1988-09-20 00:00:00
abstract：:In a large number of plant species hydroxynitrile lyases catalyze the decomposition of cyanohydrins in order to generate hydrogen cyanide upon tissue damage. Hydrogen cyanide serves as a deterrent against herbivores and fungi. In vitro hydroxynitrile lyases are proficient biocatalysts for the stereospecific synthesis ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi802162s
更新日期：2009-04-21 00:00:00
abstract：:The effect of the polyene antibiotic amphotericin B on the electron paramagnetic resonance spectra of lipid probes intercalated in model membranes was examined. When the antibiotic was added to the aqueous phase, no spectral effects occurred. However, when the antibiotic was incorporated during membrane preparation, c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00523a010
更新日期：1981-09-29 00:00:00
abstract：:Platelet receptor recognition domains are located on the gamma and alpha chains of human fibrinogen. The former encompasses residues 400-411 [Kloczewiak, M., Timmons, S., Lukas, T. J., & Hawiger, J. (1984) Biochemistry 23, 1767], and the latter is present in two loci on the alpha chain (alpha 95-97 and alpha 572-574) ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00433a026
更新日期：1989-04-04 00:00:00
abstract：:We have studied the tridecadeoxynucleotide CGCGAATTACGCG (I), which contains an additional A at position 9 compared to the dodecanucleotide of which the crystal structure has been determined. Sequence I exhibits no distinct melting curve and also has a concentration-dependent pattern of peaks on reverse-phase chromato...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00371a025
更新日期：1986-11-18 00:00:00
abstract：:Molecular imaging is an important new direction in medical diagnosis; however, its success is dependent upon molecular probes that demonstrate selective tissue targeting. We report the design and chemical synthesis of a derivative of human amyloid-beta (Abeta) peptide that is capable of selectively targeting individua...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0359574
更新日期：2004-05-25 00:00:00
abstract：:Currently, it is thought that inhalational anesthetics cause anesthesia by binding to ligand-gated ion channels. This is being investigated using four-alpha-helix bundles, small water-soluble analogues of the transmembrane domains of the "natural" receptor proteins. The study presented here specifically investigates h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034623b
更新日期：2003-09-30 00:00:00
abstract：:The reactions of Rhodopseudomonas viridis cytochrome c2 and horse cytochrome c with Rps. viridis photosynthetic reaction centers were studied by using both single- and double-flash excitation. Single-flash excitation of the reaction centers resulted in rapid photooxidation of cytochrome c-556 in the cytochrome subunit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00219a021
更新日期：1991-02-05 00:00:00
abstract：:Acyclovir triphosphate, ganciclovir triphosphate and penciclovir triphosphate inhibited DNA polymerases alpha, delta, and epsilon. Each triphosphate preferentially inhibited pol delta, although ganciclovir triphosphate was the most impressive of the three; the Ki for inhibition of pol delta was 2 microM (competitive w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00008a014
更新日期：1995-02-28 00:00:00
abstract：:By use of a mutational assay employing an octadecamer with a mismatch in the center, it is shown that the introduction of phosphorothioate groups near the 5'-end can protect the mismatch against degradation by the 5'-3'-exonuclease activity of Escherichia coli DNA polymerase I. An optimal level of protection is achiev...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00399a032
更新日期：1987-12-15 00:00:00
abstract：:The smooth muscle basic calponin interacts with F-actin and inhibits the actomyosin ATPase in a calmodulin or phosphorylation modulated manner. It also binds in vitro to microtubules and its acidic isoform, present in nonmuscle cells, and co-localizes with microfilaments and microtubules in cultured neurons. To assess...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi020336g
更新日期：2003-02-11 00:00:00
abstract：:An inhibitor of the insulin receptor tyrosine kinase (IRTK), (hydroxy-2-naphthalenyl-methyl) phosphonic acid, was designed and synthesized and was shown to be an inhibitor of the biological effects of insulin in vitro. With a wheat germ purified human placental insulin receptor preparation, this compound inhibited the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00439a053
更新日期：1989-06-27 00:00:00
abstract：:The effect of low molecular-weight compounds on the equilibrium constant K(A) can be used to explore the energetics and molecular mechanism of protein-DNA interactions. Here we use the complex composed of the integrase Tn916 DNA-binding domain and its target DNA duplex to investigate the effects of salt and the nonion...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048907n
更新日期：2005-01-11 00:00:00
abstract：:Although tryptophan synthase catalyzes a number of pyridoxal phosphate dependent beta-elimination and beta-replacement reactions that are also catalyzed by tryptophanase, a principal and puzzling difference between the two enzymes lies in the apparent inability of tryptophan synthase to catalyze beta-elimination of in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00363a010
更新日期：1986-07-29 00:00:00
abstract：:Zinc, the catalytically essential metal of angiotensin converting enzyme (ACE), has been replaced by cobalt(II) to give an active, chromophoric enzyme that is spectroscopically responsive to inhibitor binding. Visible absorption spectroscopy and magnetic circular dichroic spectropolarimetry have been used to character...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00397a014
更新日期：1987-11-17 00:00:00
abstract：:The DNA repair enzyme uracil DNA glycosylase has been crystallized with a cationic 1-aza-2'-deoxyribose-containing DNA that mimics the ultimate transition state of the reaction in which the water nucleophile attacks the anomeric center of the oxacarbenium ion-uracil anion reaction intermediate. Comparison with substra...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035372+
更新日期：2003-11-04 00:00:00
abstract：:Myelin basic protein (MBP) binds to negatively charged lipids on the cytosolic surface of oligodendrocyte membranes and is most likely responsible for adhesion of these surfaces in the multilayered myelin sheath. It can also polymerize actin, bundle F-actin filaments, and bind actin filaments to lipid bilayers through...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0519194
更新日期：2006-01-17 00:00:00
abstract：:The solution structure of a modified undecamer duplex containing (-)-(7R,8S,9R,10S)-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a] pyrene covalently bonded through trans ring opening at C10 of the epoxide by the N6-amino group of deoxyadenosine (dA) was studied. This diol epoxide 1 diastereomer has the benzylic ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00028a009
更新日期：1995-07-18 00:00:00
abstract：:While the iron-molybdenum cofactor (FeMoco) of nitrogenase, a constituent of the active site for nitrogen reduction, can be extracted into N-methylformamide (NMF) and pyrrollidinone, the inability to solubilize it in any other organic solvents has hampered further understanding of its structure and chemical properties...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00450a024
更新日期：1989-11-28 00:00:00
abstract：:A soluble Ca2+/calmodulin-dependent protein kinase has been purified from rat brain to near homogeneity by using casein as substrate. The enzyme was purified by using hydroxylapatite adsorption chromatography, phosphocellulose ion-exchange chromatography, Sepharose 6B gel filtration, affinity chromatography using calm...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00318a018
更新日期：1984-11-06 00:00:00
abstract：:Drug molecules are typically hydrophobic and small in order to traverse membranes to reach cytoplasmic targets, but we have discovered that more polar molecules can be delivered across membranes using water-soluble, moderately hydrophobic membrane peptides of the pHLIP (pH low insertion peptide) family. Delivery of po...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2009773
更新日期：2011-11-29 00:00:00
abstract：:Transcarboxylase (TC) from Propionibacterium shermanii, a biotin-dependent enzyme, catalyzes the transfer of a carboxyl group from methylmalonyl-CoA to pyruvate to form propionyl-CoA and oxalacetate. Within the multi-subunit enzyme complex, the 1.3S subunit functions as the carboxyl group carrier and also binds the ot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9925367
更新日期：2000-03-14 00:00:00
abstract：:Type II dihydrofolate reductases (DHFRs) encoded by the R67 and R388 plasmids are different both in sequence and in structure from known chromosomal DHFRs. These plasmid-derived DHFRs are responsible for conferring trimethoprim resistance to the host strain. A derivative of R388 DHFR, RBG200, has been cloned and overp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00494a011
更新日期：1990-10-23 00:00:00
abstract：:Living cells contain a range of densely phosphorylated surfaces, including phospholipid membranes, ribonucleoproteins, and nucleic acid polymers. Hyperphosphorylated surfaces also accumulate in neurodegenerative diseases as neurofibrillar tangles. We have synthesized and structurally characterized a precisely patterne...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500599a
更新日期：2014-07-08 00:00:00
abstract：:The proton nuclear magnetic resonance spectrum of human adult deoxyhemoglobin in D2O in the region from 6 to 20 ppm downfield from the proton resonance of residual water shows a number of hyperfine shifted proton resonances that are due to groups on or near the alpha and beta hemes. The sensitivity of these resonances...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00590a031
更新日期：1979-11-13 00:00:00
abstract：:The FAD-dependent alpha-glycerophosphate oxidase (GlpO) from Enterococcus casseliflavus and Streptococcus sp. was originally studied as a soluble flavoprotein oxidase; surprisingly, the GlpO sequence is 30-43% identical to those of the alpha-glycerophosphate dehydrogenases (GlpDs) from mitochondrial and bacterial sour...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701685u
更新日期：2008-01-22 00:00:00
abstract：:RNA repeat expansions cause a host of incurable, genetically defined diseases. The most common class of RNA repeats consists of trinucleotide repeats. These long, repeating transcripts fold into hairpins containing 1 × 1 internal loops that can mediate disease via a variety of mechanism(s) in which RNA is the central ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00252
更新日期：2017-07-11 00:00:00
