Abstract:
:Myelin basic protein (MBP) binds to negatively charged lipids on the cytosolic surface of oligodendrocyte membranes and is most likely responsible for adhesion of these surfaces in the multilayered myelin sheath. It can also polymerize actin, bundle F-actin filaments, and bind actin filaments to lipid bilayers through electrostatic interactions. MBP consists of a number of posttranslationally modified isomers of varying charge, some resulting from phosphorylation at several sites by different kinases, including mitogen-activated protein kinase (MAPK). Phosphorylation of MBP in oligodendrocytes occurs in response to various extracellular stimuli. Phosphorylation/dephosphorylation of MBP also occurs in the myelin sheath in response to electrical activity in the brain. Here we investigate the effect of phosphorylation of MBP on its interaction with actin in vitro by phosphorylating the most highly charged unmodified isomer, C1, at two sites with MAPK. Phosphorylation decreased the ability of MBP to polymerize actin and to bundle actin filaments but had no effect on the dissociation constant of the MBP-actin complex or on the ability of Ca2+-calmodulin to dissociate the complex. The most significant effect of phosphorylation on the MBP-actin complex was a dramatic reduction in its ability to bind to negatively charged lipid bilayers. The effect was much greater than that reported earlier for another charge isomer of MBP, C8, in which six arginines were deiminated to citrulline, resulting in a reduction of net positive charge of 6. These results indicate that although average electrostatic forces are the primary determinant of the interaction of MBP with actin, phosphorylation may have an additional effect due to a site-specific electrostatic effect or to a conformational change. Thus, phosphorylation of MBP, which occurs in response to various extracellular signals in both myelin and oligodendrocytes, attenuates the ability of MBP to polymerize and bundle actin and to bind it to a negatively charged membrane.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Boggs JM,Rangaraj G,Gao W,Heng YMdoi
10.1021/bi0519194subject
Has Abstractpub_date
2006-01-17 00:00:00pages
391-401issue
2eissn
0006-2960issn
1520-4995journal_volume
45pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Pig liver phosphomevalonate kinase (EC 2.7.4.2) has been purified to homogeneity as shown by polyacrylamide gel electrophoresis. The molecular weight estimates range from 21,000 to 22,500. Each molecule is composed of one polypeptide chain. The presence of SH-containing reagents is essential for the preservation of en...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00552a003
更新日期:1980-05-27 00:00:00
abstract::The metabolism of the anticonvulsant mephenytoin is subject to a genetic polymorphism. In 2-5% of Caucasians and 18-23% of Japanese subjects a specific cytochrome P-450 isozyme, P-450 meph, is functionally deficient or missing. We have accumulated evidence that autoimmune antibodies observed in sera of patients with t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00399a065
更新日期:1987-12-15 00:00:00
abstract::The interaction between the bacteriophage Q beta coat protein and its specific binding site on Q beta genomic RNA was characterized by using a nitrocellulose filter binding assay. Q beta coat protein bound to a synthetic 29-nucleotide RNA hairpin with an association constant of 400 microM-1 at 4 degrees C, 0.2 M ionic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00427a011
更新日期:1989-01-10 00:00:00
abstract::The tau microtubule-associated proteins are axonal proteins that have been implicated in axonal outgrowth, microtubule spacing, and microtubule bundling. Moreover, tau is the major structural component of the paired helical filaments present in the brains of Alzheimer's disease patients. The Caenorhabditis elegans Gen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952646n
更新日期:1996-07-23 00:00:00
abstract::Coiled coils (CCs) are among the best-understood protein folds. Nonetheless, there are gaps in our knowledge of CCs. Notably, CCs are likely to be structurally more dynamic than often considered. Here, we explore this in an abundant class of CCs, parallel dimers, focusing on polar asparagine (Asn) residues in the hydr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00848
更新日期:2017-12-19 00:00:00
abstract::The erythropoietin (EPO) molecule contains four carbohydrate chains. Three contain N-linkages to asparagines at positions 24, 38, and 83, and one contains an O-linkage to a serine at position 126. We constructed human EPO variants that eliminated the three N-glycosylation sites by replacing the asparagines with glutam...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00156a003
更新日期:1992-10-20 00:00:00
abstract::Methods were developed for isolation of bovine cardiac titin in the apparently full-sized T1 form. The pure protein could be obtained either in the denatured state after chromatography of myofibril extracts on Sephacryl S-1000 or in the native state using hydroxylapatite chromatography. Titin-specific chicken polyclon...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00193a012
更新日期:1994-07-12 00:00:00
abstract::The nucleotide binding capacity and affinity of the isolated beta subunit from Escherichia coli F1-ATPase have been studied with radiolabeled ADP and ATP by an equilibrium dialysis technique. Each mole of beta subunit in the presence of EDTA bound 1 mol of ADP or ATP with Kd values of 25 microM and 50-100 microM, resp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00321a048
更新日期:1984-12-18 00:00:00
abstract::Proteins have many distinct tertiary folds (Richardson, J. S. (1981) Adv. Prot. Chem. 34, 167-339). The term tertiary fold refers to the spatial organization of secondary structure elements (alpha-helices and beta-strands). It is not known when, in the process of protein folding, a native tertiary fold emerges. Here, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00174a021
更新日期:1994-03-01 00:00:00
abstract::Virulent strains of Clostridium sordellii cause gangrenous myonecrosis in humans. The released lethal toxin (TcsL) and hemorrhagic toxin (TcsH) are regarded as the major virulence factors. TcsL inactivates low molecular weight GTP-binding proteins of the Rho/Ras subfamilies by monoglucosylation. In cultured cell lines...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900964c
更新日期:2009-09-29 00:00:00
abstract::New analogues of 7-methylguanosine 5'-monophosphate (m7GMP) were synthesized with modified 5'-phosphate moieties by replacement of -O with -H, -CH3, or -NH2. Additional analogues were synthesized with 8-methyl- or 8-aminoguanine base substitutions or ring-opened ribose (2',3'-diol). These compounds were analyzed by 1H...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00388a028
更新日期:1987-07-14 00:00:00
abstract::Soluble inorganic pyrophosphatase (PPase) is one of the better understood phosphoryl-transfer enzymes and is distinctive in having four divalent metal ions at the active site. Here we determine pH profiles for wild-type Saccharomyces cerevisiae PPase (Y-PPase) and for 14 of its active site variants and consider the ef...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971771r
更新日期:1998-02-17 00:00:00
abstract::Fibroblast growth factors (FGFs) are mitogenic and chemotactic agents for a wide variety of cell types and play a primary role in the regulation of angiogenesis. Angiogenesis is involved in a variety of critical physiological events including organogenesis, wound healing, ischemic collateral circulation, and solid tum...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9521755
更新日期:1996-02-20 00:00:00
abstract::Previously, we have shown that 4-hydroxynonenal (4-HNE) induces Fas-mediated apoptosis in HLE B-3 cells through a pathway which is independent of FasL, FADD, procaspase 8, and DISC (Li, J., et al. (2006) Biochemistry 45, 12253-12264). The involvement of Daxx has also been suggested in this pathway, but its role is not...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701559f
更新日期:2008-01-08 00:00:00
abstract::The binding properties of substrates and competitive inhibitors of Escherichia coli cytidine deaminase are compared with those of the fragments obtained by cutting these ligands at several positions including the glycosidic bond. In contrast with the normal substrate cytidine (kcat/Km = 2.6 x 10(6) M-1 s-1), cytosine ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980959n
更新日期:1998-08-25 00:00:00
abstract::We have recently described a procedure for the purification of microtubule associated protein 1B (MAP1B) from calf brain [Pedrotti, B., & Islam K. (1995) Cell Motil. Cytoskeleton 30, 301-309], and this study further characterizes the purified protein and its interaction with microtubules. We show that purified MAP1B (...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951314f
更新日期:1996-03-05 00:00:00
abstract::The interaction of solvent of the substrate binding domain of the bacterial heat shock 70 chaperone protein DnaK was studied in its apo form and with bound hydrophobic substrate peptide, using refined nuclear magnetic resonance experiments. Distinct differences between the two states of the protein were observed. Acco...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi030097c
更新日期:2003-09-30 00:00:00
abstract::Site-directed mutagenesis of the aspartate receptor of Salmonella typhimurium (Tars) at serine 68, a residue located within the aspartate binding pocket and at the subunit interface, identified this residue as an allosteric switch in this receptor. Substitutions at this position can affect both the type and degree of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961481v
更新日期:1996-11-26 00:00:00
abstract::Calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. The amount of calcium liberated is proportional to the amount of bleaching in the range of 20 to 100% bleaching and is relatively insen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00626a025
更新日期:1977-04-05 00:00:00
abstract::The seven serotypes (A-G) of botulinum neurotoxins (BoNTs) function through their proteolytic cleavage of one of three proteins (SNAP-25, Syntaxin, and VAMP) that form the SNARE complex required for synaptic vesicle fusion. The different BoNTs have very specific protease recognition requirements, between 15 and 50 ami...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052518r
更新日期:2006-03-14 00:00:00
abstract::Bicyclomycin is a novel, commercially important antibiotic. Information concerning the site of bicyclomycin inhibition in Escherichia coli has been obtained by the production of bicyclomycin resistant mutants by UV irradiation. Selection by growth in the presence of bicyclomycin of a plasmid clone library generated fr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00065a007
更新日期:1993-04-13 00:00:00
abstract::Numerous zinc ectoenzymes are folded and activated in the compartments of the early secretory pathway, such as the ER and the Golgi apparatus, before reaching their final destination. During this process, zinc must be incorporated into the active site; therefore, metalation of the nascent protein is indispensable for ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00924
更新日期:2020-01-14 00:00:00
abstract::The structures of the modified folates present in Pyrococcus furiosus have been determined. This was accomplished largely by the characterization of the arylamines resulting from the air oxidative cleavage of the reduced modified folates present in these cells, using both chemical and enzymatic methods. Cell extracts ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00054a003
更新日期:1993-01-26 00:00:00
abstract::Inhibition of gamma-aminobutyric acid aminotransferase (GABA-AT) increases the concentration of GABA, an inhibitory neurotransmitter in human brain, which could have therapeutic applications for a variety of neurological diseases, including epilepsy. On the basis of studies of several previously synthesized conformati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061592m
更新日期:2006-12-05 00:00:00
abstract::The melibiose permease of Salmonella typhimurium (MelB(St)) catalyzes symport of melibiose with Na(+), Li(+), or H(+), and bioinformatics analysis indicates that a conserved Gly117 (helix IV) is part of the Na(+)-binding site. We mutated Gly117 to Ala, Pro, Trp, or Arg; the effects on melibiose transport and binding o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300230h
更新日期:2012-04-03 00:00:00
abstract::The Wiskott-Aldrich syndrome protein (WASP) and neural WASP (N-WASP) are key players in regulating actin cytoskeleton via the Arp2/3 complex. It has been widely reported that the WASP proteins are activated by Rho family small GTPase Cdc42 and that Rac1 acts through SCAR/WAVE proteins. However, a systematic study of t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi062152y
更新日期:2007-03-20 00:00:00
abstract::TPPP/p25 is a brain-specific protein, which induces tubulin polymerization and microtubule (MT) bundling and is enriched in Lewy bodies characteristic of Parkinson's disease [Tirián et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 13976-13981]. We identified two human gene sequences, CG1-38 and p25beta, which encoded ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061305e
更新日期:2006-11-21 00:00:00
abstract::Azide binds to fast cytochrome bo with a stoichiometry of 1:1, the dissociation constant for this reaction being approximately 2 x 10(-5) M. The changes induced in the electronic absorption are very slight and are consistent with heme o remaining hexacoordinate high-spin, an observation confirmed by room temperature M...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961221d
更新日期:1996-10-29 00:00:00
abstract::Clostridium perfringens epsilon toxin belongs to the aerolysin-like family of pore-forming toxins and is one of the most potent bacterial toxins known. The epsilon toxin causes fatal enterotoxemia in sheep, goats, and possibly humans. Evidence indicates that the toxin binds to protein receptors including hepatitis A v...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300690a
更新日期:2012-09-25 00:00:00
abstract::Xylose isomerase (E.C. 5.3.1.5) catalyzes the interconversion of aldose and ketose sugars and has an absolute requirement for two divalent cations at its active site to drive the hydride transfer rates of sugar isomerization. Evidence suggests some degree of metal movement at the second metal site, although how this m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049812o
更新日期:2004-06-01 00:00:00