High resolution solution structure of the 1.3S subunit of transcarboxylase from Propionibacterium shermanii.

abstract：:The influence of DNA base sequence context on the removal of a bulky benzo[a]pyrene diol epoxide-guanine adduct, (+)-trans-B[a]P-N2-dG (G*), by UvrABC nuclease from the thermophilic organism Bacillus caldotenax was investigated. The lesion was flanked by either T or C in otherwise identical complementary 43-mer duplex...

journal_title：Biochemistry

authors： Ruan Q,Liu T,Kolbanovskiy A,Liu Y,Ren J,Skorvaga M,Zou Y,Lader J,Malkani B,Amin S,Van Houten B,Geacintov NE

更新日期：2007-06-12 00:00:00

abstract：:Polyamides composed of N-methylpyrrole (Py) and N-methylimidazole (Im) subunits can bind in the minor groove of DNA at predetermined sequences with subnanomolar affinity and high specificity. Covalent linkage of polymer subunits using a gamma-aminobutyric acid linker has been shown to increase both the affinity and sp...

journal_title：Biochemistry

authors： Baliga R,Baird EE,Herman DM,Melander C,Dervan PB,Crothers DM

更新日期：2001-01-09 00:00:00

abstract：:The structures at 2.0 and 2.25 A resolution of native and recombinant nitrite reductase from Alcaligenes faecalis show that they are identical to each other and very similar to nitrite reductase from Achromobacter cycloclastes. The crystallographic structure of a mutant, M150E, which unlike the wild-type protein canno...

journal_title：Biochemistry

authors： Murphy ME,Turley S,Kukimoto M,Nishiyama M,Horinouchi S,Sasaki H,Tanokura M,Adman ET

更新日期：1995-09-26 00:00:00

abstract：:Cell free protein synthesis (CFPS) has emerged as a promising methodology for protein expression. While polypeptide production is very reliable and efficient using CFPS, the correct cotranslational folding of membrane proteins during CFPS is still a challenge. In this contribution, we describe a two-step protocol in w...

journal_title：Biochemistry

authors： Focke PJ,Hein C,Hoffmann B,Matulef K,Bernhard F,Dötsch V,Valiyaveetil FI

更新日期：2016-08-02 00:00:00

abstract：:β-Barrel membrane proteins are found in the outer membranes of mitochondria, chloroplasts, and Gram-negative bacteria; however, exactly how they are folded and inserted remains unknown. Over the past decade, both functional and structural studies have greatly contributed to addressing this elusive mechanism. It is kno...

journal_title：Biochemistry

authors： O'Neil PK,Rollauer SE,Noinaj N,Buchanan SK

更新日期：2015-10-20 00:00:00

abstract：:Neutral solutes were used to investigate the effects of osmotic stress both on the ability of rhodopsin to undergo its activating conformation change and on acyl chain packing in the rod outer segment (ROS) disk membrane. The equilibrium concentration of metarhodopsin II (MII), the conformation of photoactivated rhodo...

journal_title：Biochemistry

authors： Mitchell DC,Litman BJ

更新日期：1999-06-15 00:00:00

abstract：:Thrombin-activatable fibrinolysis inhibitor (TAFI) circulates as an inactive proenzyme of a carboxypeptidase B-like enzyme (TAFIa). It functions by removing C-terminal lysine residues from partially degraded fibrin that are important in tissue-type plasminogen activator mediated plasmin formation. TAFI was classified ...

journal_title：Biochemistry

authors： Marx PF,Bouma BN,Meijers JC

更新日期：2002-01-29 00:00:00

abstract：:The ferlin family proteins have emerged as multi-C2 domain regulators of calcium-triggered membrane fusion and fission events. While initially determined to share many of the features of members of the synaptotagmin family of calcium sensors, ferlins in more recent studies have been found to interact directly with non...

journal_title：Biochemistry

authors： Johnson CP

更新日期：2017-12-12 00:00:00

abstract：:The local environment of the transmembrane and C-terminal domain of M13 major coat protein was probed by site-directed ESR spin labeling when the protein was introduced into three membrane-mimicking systems, DOPC vesicles, sodium cholate micelles, and SDS micelles. For this purpose, we have inserted unique cysteine re...

journal_title：Biochemistry

authors： Stopar D,Spruijt RB,Wolfs CJ,Hemminga MA

更新日期：1996-12-03 00:00:00

abstract：:FtmPT1 is a fungal indole prenyltransferase that catalyzes the reaction of tryptophan derivatives with dimethylallyl pyrophosphate to form various biologically active compounds. Herein, we describe detailed studies of FtmPT1 catalysis involving dimethylallyl pyrophosphate and Brevianamide F following the native pathwa...

journal_title：Biochemistry

authors： Pan LL,Yang Y,Merz KM Jr

更新日期：2014-09-30 00:00:00

abstract：:A model cosolvent, ethanol, has profound and diversified effects on the amyloidogenic self-assembly of insulin, yielding spectroscopically and morphologically distinguishable forms of beta-aggregates. The alcohol reduces hydrodynamic radii of insulin molecules, decreases enthalpic costs associated with aggregation-pro...

journal_title：Biochemistry

authors： Dzwolak W,Grudzielanek S,Smirnovas V,Ravindra R,Nicolini C,Jansen R,Loksztejn A,Porowski S,Winter R

更新日期：2005-06-28 00:00:00

abstract：:Database searches indicated that the genome of Bacillus subtilis contains three different genes encoding RNase H homologues. The ypdQ gene encodes an RNase HI homologue with 132 amino acid residues, whereas the rnh and ysgB genes encode RNase HII homologues with 255 and 313 amino acid residues, respectively. RNases HI...

journal_title：Biochemistry

authors： Ohtani N,Haruki M,Morikawa M,Crouch RJ,Itaya M,Kanaya S

更新日期：1999-01-12 00:00:00

abstract：:An RNA that replicates with core RNA polymerase from E. coli and the substrates ATP, CTP, ITP, and UTP, was selected from a random poly(A,U,I,C) library and named EcorpI. Another replicating RNA, EcorpG, was obtained by template-free incubation of holo RNA polymerase and the substrates ATP, CTP, GTP, and UTP. Both RNA...

journal_title：Biochemistry

authors： Wettich A,Biebricher CK

更新日期：2001-03-20 00:00:00

abstract：:A high degree of selectivity toward the target site of the pest organism is a desirable attribute for new safer agrochemicals. To assist in the design of novel herbicides, we determined the crystal structures of the herbicidal target enzyme 4-hydroxyphenylpyruvate dioxygenase (HPPD; EC 1.13.11.27) from the plant Arabi...

journal_title：Biochemistry

authors： Yang C,Pflugrath JW,Camper DL,Foster ML,Pernich DJ,Walsh TA

更新日期：2004-08-17 00:00:00

abstract：:To probe the mechanism of chaperone substrate selection, we have investigated the kinetics of complex formation and dissociation between the molecular chaperone DnaK and a short peptide (Cro, representing amino acids 1-12 of the cro repressor protein). The Cro protein was N-terminally labeled with the environmentally ...

journal_title：Biochemistry

authors： Farr CD,Galiano FJ,Witt SN

更新日期：1995-11-28 00:00:00

abstract：:Pterin-free inducible nitric oxide synthase (iNOS) was reconstituted with tetrahydrobiopterin (H(4)B) or tetrahydrobiopterin analogues (5-methyl-H(4)B and 4-amino-H(4)B), and the ability of bound 5-methyl-H(4)B and 4-amino-H(4)B to support catalysis by either full-length iNOS (FLiNOS) or the isolated heme domain (HDiN...

journal_title：Biochemistry

authors： Hurshman AR,Krebs C,Edmondson DE,Marletta MA

更新日期：2003-11-18 00:00:00

abstract：:To better understand the factors controlling the binding of apolipoprotein molecules at the surfaces of serum lipoprotein particles, the adsorption of human apolipoprotein A-I to phospholipid monolayers has been studied. The influence of lipid packing was investigated by spreading the monolayers at various initial sur...

journal_title：Biochemistry

authors： Ibdah JA,Phillips MC

更新日期：1988-09-06 00:00:00

abstract：:Aromatic residues in c-type cytochromes might have an important function in the folding and/or electron transferring properties of the molecule. In the tetraheme cytochrome c3 (Mr 13 000) from Desulfovibrio vulgaris Hildenborough, Phe20, is located between heme 1 and heme 3 with its aromatic ring close and almost para...

journal_title：Biochemistry

authors： Dolla A,Arnoux P,Protasevich I,Lobachov V,Brugna M,Giudici-Orticoni MT,Haser R,Czjzek M,Makarov A,Bruschi M

更新日期：1999-01-05 00:00:00

abstract：:A detailed interpretation of the grossly distorted ultraviolet absorption and circular dichroism spectra of the intact red blood cell is given, including an evaluation of the effects of protein conformation, detector geometry, cell hemoglobin content, and refractive index on the calculated cell spectra. The origins of...

journal_title：Biochemistry

authors： Gitter-Amir A,Schneider AS,Rosenheck K

更新日期：1976-07-13 00:00:00

abstract：:HpaI and BphI are two pyruvate class II aldolases found in aromatic meta-cleavage degradation pathways that catalyze similar reactions but are not related in sequence. Steady-state kinetic analysis of the aldol addition reactions and product inhibition assays showed that HpaI exhibits a rapid equilibrium random order ...

journal_title：Biochemistry

authors： Wang W,Baker P,Seah SY

更新日期：2010-05-04 00:00:00

abstract：:Solvent reorganization can contribute significantly to the energetics of protein-protein interactions. However, our knowledge of the magnitude of the energetic contribution is limited, in part, by a dearth of quantitative experimental measurements. The biotin repressor forms a homodimer as a prerequisite to DNA bindin...

journal_title：Biochemistry

authors： Eginton C,Beckett D

更新日期：2013-09-24 00:00:00

abstract：:Gelatinase B is a matrix metalloproteinase (MMP-9) involved in tissue remodeling, development, cancer, and inflammation. Neutrophils produce three major forms of (pro)gelatinase B: 92 kDa monomers, homodimers, and complexes of gelatinase B covalently bound to neutrophil gelatinase B-associated lipocalin (NGAL). In con...

journal_title：Biochemistry

authors： Rudd PM,Mattu TS,Masure S,Bratt T,Van den Steen PE,Wormald MR,Küster B,Harvey DJ,Borregaard N,Van Damme J,Dwek RA,Opdenakker G

更新日期：1999-10-19 00:00:00

abstract：:Human erythrocyte spectrin prepared from fresh blood is a mixture of different association states. Depending on the manner of preparation, the two-chain dimer or the tetramer predominates. These forms are not in rapid thermodynamic equilibrium. The molecular weight of the dimer by sedimentation and diffusion and by li...

journal_title：Biochemistry

authors： Kam Z,Josephs R,Eisenberg H,Gratzer WB

更新日期：1977-12-13 00:00:00

abstract：:The heme binding sites of rabbit histidine-rich glycoprotein (HRG), 94 kDa, were studied with rose bengal (RB), a fluorescein derivative that associates with histidine residues. Difference absorbance spectra indicate that HRG binds RB at two thermodynamically preferred sites (Kd approximately 2 microM) that are spectr...

journal_title：Biochemistry

authors： Burch MK,Morgan WT

更新日期：1985-10-08 00:00:00

abstract：:Two forms of cytochrome P-450, designated P-450NMa and P-450NMb, were purified to electrophoretic homogeneity from rabbit nasal microsomes. The purified cytochromes, which contained 14-16 nmol of P-450/mg of protein, exhibited apparent monomeric molecular weights of 49,500 and 51,000, respectively. As indicated by sev...

journal_title：Biochemistry

authors： Ding XX,Coon MJ

更新日期：1988-11-01 00:00:00

abstract：:The environmentally sensitive fluorescent probes 6-propionyl-2-(N,N-dimethylamino)naphthalene (PRODAN) and 2'-(N,N-dimethylamino)-6-naphthoyl-4-trans-cyclohexanioc acid (DANCA) form complexes with the heme binding site of apohorseradish peroxidase. The dissociation constants of the PRODAN and DANCA complexes were dete...

journal_title：Biochemistry

authors： Lasagna M,Vargas V,Jameson DM,Brunet JE

更新日期：1996-01-23 00:00:00

abstract：:The Haemophilus influenzae diaminopimelate epimerase was cloned, expressed, purified, and crystallized in the C2221 space group (a = 102.1 A, b = 115.4 A, c = 66.3 A, alpha = beta = gamma = 90 degrees). The three-dimensional structure was solved to 2.7 A using a single Pt derivative and the Se-Met-substituted enzyme t...

journal_title：Biochemistry

authors： Cirilli M,Zheng R,Scapin G,Blanchard JS

更新日期：1998-11-24 00:00:00

abstract：:The mitogen-activated protein kinases (MAPKs) are key components of cellular signal transduction pathways, which are down-regulated by the MAPK phosphatases (MKPs). Catalytic activity of the MKPs is controlled both by their ability to recognize selective MAPKs and by allosteric activation upon binding to MAPK substrat...

journal_title：Biochemistry

authors： Lu C,Liu X,Zhang CS,Gong H,Wu JW,Wang ZX

更新日期：2017-11-21 00:00:00

abstract：:Phage display technique is a powerful tool with which to identify novel binding sequences for antibody and receptor targets. Few studies, however, have used this technology to select affinity peptides for ligand molecules. Here, we screened a peptide phage library for binding to toxic shock syndrome toxin 1 (TSST-1) t...

journal_title：Biochemistry

authors： Sato A,Ida N,Fukuyama M,Miwa K,Kazami J,Nakamura H

更新日期：1996-08-13 00:00:00

abstract：:Peptidylglycine monooxygenase (PHM) catalyzes the final step in the biosynthesis of amidated peptides that serve as important signaling molecules in numerous endocrine pathways. The catalytic mechanism has attracted much attention because of a number of unique attributes, including the presence of a pair of uncoupled ...

journal_title：Biochemistry

authors： Chauhan S,Kline CD,Mayfield M,Blackburn NJ

更新日期：2014-02-18 00:00:00