Abstract:
:Plasma membrane and vesicular transporters for the biogenic amines, dopamine, norepinephrine, and serotonin, represent a group of proteins that play a crucial role in the regulation of neurotransmission. Clinically, mono amine transporters are the primary targets for the actions of many therapeutic agents used to treat mood disorders, as well as the site of action for highly addictive psychostimulants such as cocaine, amphetamine, methamphetamine, and 3,4-methylenedioxymethamphetamine. Over the past decade, the use of approaches such as yeast two-hybrid and proteomics has identified a multitude of transporter interacting proteins, suggesting that the function and regulation of these transporters are more complex than previously anticipated. With the increasing number of interacting proteins, the rules dictating transporter synthesis, assembly, targeting, trafficking, and function are beginning to be deciphered. Although many of these protein interactions have yet to be fully characterized, current knowledge is beginning to shed light on novel transporter mechanisms involved in monoamine homeostasis, the molecular actions of psychostimulants, and potential disease mechanisms. While future studies resolving the spatial and temporal resolution of these, and yet unknown, interactions will be needed, the realization that monoamine transporters do not work alone opens the path to a plethora of possible pharmacological interventions.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Sager JJ,Torres GEdoi
10.1021/bi200405csubject
Has Abstractpub_date
2011-08-30 00:00:00pages
7295-310issue
34eissn
0006-2960issn
1520-4995journal_volume
50pub_type
杂志文章,评审相关文献
BIOCHEMISTRY文献大全abstract::Guanidine hydrochloride (GdnHCl) and thermally induced unfolding measurements on the oxidized form of Escherichia coli thioredoxin at pH 7 were combined for the purpose of assessing the functional dependence of unfolding free energy changes on denaturant concentration over an extended GdnHCl concentration range. Conve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00135a022
更新日期:1992-05-26 00:00:00
abstract::Here we explore the use of ethidium to determine relative affinities of different gene delivery vectors for DNA and describe an improved method for studying the interaction. Specifically, we investigate the binding of poly(amidoamine) dendrimers and show that the DNA-dendrimer-ethidium system is far from thermodynamic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1015887
更新日期:2011-02-22 00:00:00
abstract::Sulfonylureas and glinides are commonly used oral insulin secretagogues (ISs) that act on the pancreatic ATP-sensitive potassium (KATP) channel to promote insulin secretion in order to lower the blood glucose level. Physiologically, KATP channels are inhibited by intracellular ATP and activated by Mg-ADP. Therefore, t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00692
更新日期:2020-01-14 00:00:00
abstract::The binding of heparin to high molecular weight kininogen (H-kininogen) was analyzed by the effect of kininogen in decreasing the heparin-induced enhancement of the rate of inactivation of thrombin by antithrombin. The conditions were arranged so that the heparin-catalyzed antithrombin-thrombin reaction, monitored in ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00429a039
更新日期:1989-02-07 00:00:00
abstract::The Co and Fe K-edge extended X-ray absorption fine structure (EXAFS) spectra of the methionyl aminopeptidase from Escherichia coli (EcMetAP) have been recorded in the presence of 1 and 2 equiv of either Co(II) or Fe(II) (i.e., [Co(II)_(EcMetAP)], [Co(II)Co(II)(EcMetAP)], [Fe(II)_(EcMetAP)], and [Fe(II)Fe(II)(EcMetAP)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010837m
更新日期:2001-11-06 00:00:00
abstract::The thermodynamic and activation parameters for the reduction of delta 1-pyrroline-2-carboxylic acid (an alpha-imino acid) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) are compared with those for the reduction of the same imino acid by the glutamate dehydrogenase-NADPH complex. The enthalpies of acti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00341a013
更新日期:1985-09-24 00:00:00
abstract::The membrane-active, cationic, β-hairpin peptide, arenicin, isolated from marine polychaeta Arenicola marina exhibits a broad spectrum of antimicrobial activity. The peptide in aqueous solution adopts the significantly twisted β-hairpin conformation without pronounced amphipathicity. To assess the mechanism of arenici...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200746t
更新日期:2011-07-19 00:00:00
abstract::The heat-stable, protein inhibitor of the cyclic adenosine monophosphate (cAMP) dependent protein kinase [Walsh, D. A., Ashby, C. D., Gonzalez, C., Calkins, D., Fischer, E., & Krebs, E (1971a) J. Biol. Chem. 246, 1977-1985] has been purified to homogeneity from rabbit skeletal muscle by preparative electrophoresis. Em...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00589a011
更新日期:1979-10-30 00:00:00
abstract::Ks values for binding of selected substrates, competitive inhibitors, and a noncompetitive inhibitor were found to be similar for alpha-chymotrypsin and N-methyl-alpha-chymotrypsin. The rates and steps of binding of a competitive inhibitor and a noncompetitive inhibitor were also found to be similar for alpha-chymotry...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00262a017
更新日期:1982-09-14 00:00:00
abstract::Escherichia coli (A)BC excinuclease is the major enzyme responsible for removing bulky adducts, such as pyrimidine dimers and 6-4 photoproducts, from DNA. Mutants deficient in this enzyme are extremely sensitive to UV and UV-mimetic agents, but not to oxidizing agents, or ionizing radiation which damages DNA in part b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00446a002
更新日期:1989-10-03 00:00:00
abstract::Inosine has the ability to act as a "wild-card" binding nonspecifically to both A.T and G.C base pairs. This has obvious implications for the design of oligonucleotide site-directed probes. In this paper we present a series of oligonucleotides with a 5'pur9-pyr9-pyr9 motif which are designed to fold up sequentially in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960193w
更新日期:1996-10-15 00:00:00
abstract::Intracellular retinoid-binding proteins are small, tightly folded, compact proteins, which appear to be involved in the delivery of retinoids to microsomal metabolic enzymes, among other potential roles. Recently, it has been demonstrated that two of these binding proteins, cellular retinol-binding protein (CRBP) and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00176a017
更新日期:1994-03-15 00:00:00
abstract::Different sites of the tRNA molecule influence the activity of the elongation factor Tu (EF-Tu) center for GTP hydrolysis [Parlato, G., Pizzano, R., Picone, D., Guesnet, J., Fasano, O., & Parmeggiani, A. (1983) J. Biol. Chem. 258, 995-1000]. Continuing these studies, we have investigated some aspects of (a) the effect...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00427a044
更新日期:1989-01-10 00:00:00
abstract::In many crustaceans, changing concentrations of several low molecular weight compounds modulates hemocyanin oxygen binding, resulting in lower or higher oxygen affinities of the pigment. The nonphysiological effector caffeine and the physiological modulator urate, the latter accumulating in the hemolymph of the lobste...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050507s
更新日期:2005-08-02 00:00:00
abstract::ZFY, a male-associated Zn-finger protein encoded by the human Y chromosome, exhibits a distinctive two-finger repeat: whereas odd-numbered domains fit a general consensus, even-numbered domains exhibit systematic differences. Do these odd and even sequences encode structurally distinct surfaces for DNA recognition? As...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00243a005
更新日期:1991-07-23 00:00:00
abstract::Pyruvate dehydrogenase kinase 2 (PDK2) activity is enhanced by the dihydrolipoyl acetyltransferase core (E2 60mer) that binds PDK2 and a large number of its pyruvate dehydrogenase (E1) substrate. With E2-activated PDK2, K(+) at approximately 90 mM and Cl(-) at approximately 60 mM decreased the K(m) of PDK2 for ATP and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049488x
更新日期:2004-10-26 00:00:00
abstract::Indolethylamine-N-methyltransferase (INMT) is a Class 1 transmethylation enzyme known for its production of N,N-dimethyltryptamine (DMT), a hallucinogen with affinity for various serotonergic, adrenergic, histaminergic, dopaminergic, and sigma-1 receptors. DMT is produced via the action of INMT on the endogenous subst...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500175p
更新日期:2014-05-13 00:00:00
abstract::Five gangliosides, accounting for over 95% of the total ganglioside fraction, were isolated from bovine adrenal medulla by preparative thin-layer chromatography and the carbohydrate structures determined by a combination of periodate oxidation and permethylation techniques. Partially methylated alditol acetates were g...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00678a026
更新日期:1975-04-08 00:00:00
abstract::The WSXWS motif is a highly conserved structural feature of the type I cytokine receptor family. It has previously been demonstrated that mutations in the (232)WSAWS(236) motif in the erythropoietin receptor (EPOR) can result in strongly inhibited surface expression, due to defective intracellular transport [Hilton, D...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034112p
更新日期:2003-07-22 00:00:00
abstract::The two disparate functions of DCoH1 (dimerization cofactor of HNF-1)/PCD (pterin-4a-carbinolamine dehydratase) are associated with a change in oligomeric state. DCoH dimers enhance the activity of the diabetes-associated transcription factor HNF-1α (hepatocyte nuclear factor-1α), while the PCD activity of DCoH1 homot...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1015056
更新日期:2010-11-30 00:00:00
abstract::The solution structure of the 63-residue heregulin-alpha (HRG-alpha) epidermal growth factor (EGF)-like domain, corresponding to residues 177-239 of HRG-alpha, has been determined to high resolution using data from two-dimensional and three-dimensional homo- and heteronuclear NMR spectroscopy. The structure is based o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952626l
更新日期:1996-03-19 00:00:00
abstract::The importance of a specific hydrogen bond between thermolysin and a phosphonamidate inhibitor, Z-NHCH2-PO(O-)-Leu-Leu (1) [Bartlett, P. A., & Marlowe, C. K. (1987) Science (Washington D.C.) 235, 569-571], has been reevaluated. We have determined the inhibition constants (binding free energies) for thermolysin of phos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00438a006
更新日期:1989-06-13 00:00:00
abstract::Z-Ala-Ala-Phe-glyoxal (where Z is benzyloxycarbonyl) has been shown to be a competitive inhibitor of pepsin with a Ki = 89 +/- 24 nM at pH 2.0 and 25 degrees C. Both the ketone carbon (R13COCHO) and the aldehyde carbon (RCO13CHO) of the glyoxal group of Z-Ala-Ala-Phe-glyoxal have been 13C-enriched. Using 13C NMR, it h...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701000k
更新日期:2007-10-02 00:00:00
abstract::Thioltransferase activity was identified and the enzyme purified to apparent homogeneity from human red blood cells. Activity was measured as glutathione-dependent reduction of the prototype substrate hydroxyethyl disulfide; formation of oxidized glutathione (GSSG) was coupled to NADPH oxidation by GSSG reductase (1 u...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00239a002
更新日期:1991-06-25 00:00:00
abstract::Members of the ATP-binding cassette (ABC) transporters share the same basic architecture, with a four-core domain made of two transmembrane plus two nucleotide-binding domains. However, a supramolecular organization has been detected in some ABC transporters, which might be relevant to physiological regulation of subs...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0482809
更新日期:2005-03-22 00:00:00
abstract::The channel of the purified acetylcholine receptor from Torpedo californica electric organ reconstituted in lipid vesicles was assayed by direct electrical recording using patch-clamp pipets. High-resistance seals were obtained by gentle suction of vesicles into the pipet or after the formation of lipid bilayers from ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00279a003
更新日期:1983-05-10 00:00:00
abstract::Interleukin-8 (IL-8), a member of the chemokine superfamily, exists as both monomers and dimers, and mediates its function by binding to neutrophil CXCR1 and CXCR2 receptors that belong to the G protein-coupled receptor class. It is now well established that the monomer functions as a high-affinity ligand, but the bin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0605944
更新日期:2006-06-27 00:00:00
abstract::The primary structure of a multifunctional protein, the large alpha-subunit of the Escherichia coli fatty acid oxidation complex, was determined by sequencing the fadB region of the fadBA operon. The amino-terminal sequence of this protein had been established by Edman degradation. The transcription start site of the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00241a023
更新日期:1991-07-09 00:00:00
abstract::To investigate the effect of a reduced level of selenocysteine (Sec) tRNA[Ser]Sec in selenoprotein biosynthesis, two mouse embryonic stem (ES) cell lines heterozygous for the corresponding gene were generated by homologous recombination of the host genome with targeting vectors encoding a deleted or a disrupted tRNA[S...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970608t
更新日期:1997-07-15 00:00:00
abstract::The cyclic decapeptide antibiotic tyrocidine A was studied by two relatively new methods, viz., correlation proton magnetic resonance (pmr) spectroscopy and double-resonance difference pmr spectroscopy. The correlation method of spectral accumulation provided pmr spectra of good resolution, and in addition the signal-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00673a032
更新日期:1975-01-28 00:00:00